Restriction map for
pF1KE0681
(Circular) (Six-base) MAPSORT of: pF1KE0681.seq Check: 7290 from: 1 to: 3923
pF1KE0681 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 384 457 1748 2180 2927 3195 349
Size: 35 73 1291 432 747 268 1077
Fragments arranged by size:
1291 1077 747 432 268 73 35
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AleI CACnn'nnGTG
Cuts at: 298 559 298
Size: 261 3662
AlwNI CAG_nnn'CTG
Cuts at: 435 1228 2795 435
Size: 793 1567 1563
Fragments arranged by size:
1567 1563 793
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 943 943
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 937 1967 937
Size: 1030 2893
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbsI GAAGACnn'nnnn_
Cuts at: 791 791
Size: 3923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 754 788 754
Size: 34 3889
BciVI GTATCCnnnnn_n'
Cuts at: 155 1821 2582 3923 155
Size: 1666 761 1341 155
Fragments arranged by size:
1666 1341 761 155
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 777 1548 1641 2697 777
Size: 771 93 1056 2003
Fragments arranged by size:
2003 1056 771 93
BmrI ACTGGGnnnn_n'
Cuts at: 111 1135 1216 1551 3695 111
Size: 1024 81 335 2144 339
Fragments arranged by size:
2144 1024 339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
Bpu10I CC'TnA_GC
Cuts at: 422 422
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 612 798 3627 612
Size: 186 2829 908
Fragments arranged by size:
2829 908 186
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 402 432 778 808 402
Size: 30 346 30 3517
Fragments arranged by size:
3517 346 30 30
BseYI C'CCAG_C
Cuts at: 891 1348 2106 2683 3179 891
Size: 457 758 577 496 1635
Fragments arranged by size:
1635 758 577 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 883 847
Size: 36 3887
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 937 1715 1905 2697 937
Size: 778 190 792 2163
Fragments arranged by size:
2163 792 778 190
BsmI GAATG_Cn'
Cuts at: 729 890 729
Size: 161 3762
BsmBI CGTCTCn'nnnn_
Cuts at: 797 797
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 777 937 1548 1641 1715 1905 1967 2697 777
Size: 160 611 93 74 190 62 730 2003
Fragments arranged by size:
2003 730 611 190 160 93 74 62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstXI CCAn_nnnn'nTGG
Cuts at: 682 682
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 296 296
Size: 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 584 1873 2054 2217 584
Size: 1289 181 163 2290
Fragments arranged by size:
2290 1289 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DraIII CAC_nnn'GTG
Cuts at: 603 603
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 705 817 1629 2487 705
Size: 112 812 858 2141
Fragments arranged by size:
2141 858 812 112
EaeI y'GGCC_r
Cuts at: 1508 1682 2073 2100 3445 1508
Size: 174 391 27 1345 1986
Fragments arranged by size:
1986 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 433 1946 2156 433
Size: 1513 210 2200
Fragments arranged by size:
2200 1513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 384 457 1748 2180 2927 3195 349
Size: 35 73 1291 432 747 268 1077
Fragments arranged by size:
1291 1077 747 432 268 73 35
EcoO109I rG'GnC_Cy
Cuts at: 746 1062 746
Size: 316 3607
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 1942 306
Size: 1636 2287
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 611 643 778 810 1765 1795 1797 1827 611
Size: 32 135 32 955 30 2 30 2707
Fragments arranged by size:
2707 955 135 32 32 30 30 2
HincII GTy'rAC
Cuts at: 162 822 962 162
Size: 660 140 3123
Fragments arranged by size:
3123 660 140
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 1684 1684
Size: 3923
MslI CAynn'nnrTG
Cuts at: 298 559 1912 2039 298
Size: 261 1353 127 2182
Fragments arranged by size:
2182 1353 261 127
MspA1I CmG'CkG
Cuts at: 1034 1348 1708 2721 2966 1034
Size: 314 360 1013 245 1991
Fragments arranged by size:
1991 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 693 976 1476 2383 693
Size: 283 500 907 2233
Fragments arranged by size:
2233 907 500 283
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PfoI T'CCnGG_A
Cuts at: 648 648
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PpuMI rG'GwC_Cy
Cuts at: 746 746
Size: 3923
PstI C_TGCA'G
Cuts at: 970 1655 970
Size: 685 3238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 1348 1708 1348
Size: 360 3563
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 427 966 1651 2644 2835 33
Size: 394 539 685 993 191 1121
Fragments arranged by size:
1121 993 685 539 394 191
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StyI C'CwwG_G
Cuts at: 675 1057 1411 675
Size: 382 354 3187
Fragments arranged by size:
3187 382 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 1506 1639 2098 2307 571
Size: 935 133 459 209 2187
Fragments arranged by size:
2187 935 459 209 133
TatI w'GTAC_w
Cuts at: 362 3438 362
Size: 3076 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 402 434 3440 3472 125
Size: 32 245 32 3006 32 576
Fragments arranged by size:
3006 576 245 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 790 1720 790
Size: 930 2993
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
XmnI GAAnn'nnTTC
Cuts at: 320 320
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI BseRI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI