Restriction map for
pF1KE0678
(Circular) (Six-base) MAPSORT of: pF1KE0678.seq Check: 9787 from: 1 to: 4301
pF1KE0678 4301 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 449 449
Size: 4301
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2558 3305 3573 2126
Size: 432 747 268 2854
Fragments arranged by size:
2854 747 432 268
AflIII A'CryG_T
Cuts at: 392 815 1141 1653 2757 3734 392
Size: 423 326 512 1104 977 959
Fragments arranged by size:
1104 977 959 512 423 326
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AhdI GACnn_n'nnGTC
Cuts at: 726 726
Size: 4301
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 379
Size: 32 4269
AlwNI CAG_nnn'CTG
Cuts at: 339 1606 3173 339
Size: 1267 1567 1467
Fragments arranged by size:
1567 1467 1267
ApaLI G'TGCA_C
Cuts at: 586 3071 586
Size: 2485 1816
ApoI r'AATT_y
Cuts at: 179 1305 3654 3910 179
Size: 1126 2349 256 570
Fragments arranged by size:
2349 1126 570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1321 1321
Size: 4301
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 652 669 1317 1731 1979 2014 652
Size: 17 648 414 248 35 2939
Fragments arranged by size:
2939 648 414 248 35 17
BanII G_rGCy'C
Cuts at: 1315 2345 1315
Size: 1030 3271
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 303 395 1138 303
Size: 92 743 3466
Fragments arranged by size:
3466 743 92
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 584 590 1926 2019 3075 584
Size: 6 1336 93 1056 1810
Fragments arranged by size:
1810 1336 1056 93 6
BmrI ACTGGGnnnn_n'
Cuts at: 972 1513 1594 1929 4073 972
Size: 541 81 335 2144 1200
Fragments arranged by size:
2144 1200 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 361 672 771 307
Size: 54 311 99 3837
Fragments arranged by size:
3837 311 99 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1477 2356 2848 3146 1066
Size: 411 879 492 298 2221
Fragments arranged by size:
2221 879 492 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 419 762 4005 419
Size: 343 3243 715
Fragments arranged by size:
3243 715 343
BsaAI yAC'GTr
Cuts at: 585 1654 585
Size: 1069 3232
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1490 2011 2689 2963 3110 3521 1490
Size: 521 678 274 147 411 2270
Fragments arranged by size:
2270 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 417 1726 2484 3061 3557 417
Size: 1309 758 577 496 1161
Fragments arranged by size:
1309 1161 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 590 1160 1315 2093 2283 3075 347
Size: 243 570 155 778 190 792 1573
Fragments arranged by size:
1573 792 778 570 243 190 155
BsmBI CGTCTCn'nnnn_
Cuts at: 817 817
Size: 4301
Bsp1286I G_dGCh'C
Cuts at: 347 584 590 1160 1315 1926 2019 2093 2283
Size: 237 6 570 155 611 93 74 190
Cuts at: 2283 2345 3075 347
Size: 62 730 1573
Fragments arranged by size:
1573 730 611 570 237 190 155 93
74 62 6
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 449 524 1351 1565 1867 2248 437
Size: 12 75 827 214 302 381 2490
Fragments arranged by size:
2490 827 381 302 214 75 12
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 263 300 605 2213 263
Size: 37 305 1608 2351
Fragments arranged by size:
2351 1608 305 37
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BsrGI T'GTAC_A
Cuts at: 225 810 225
Size: 585 3716
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstYI r'GATC_y
Cuts at: 1 1326 1793 1816 2151 2397 3409 3495 3507
Size: 1325 467 23 335 246 1012 86 12
Cuts at: 3507 3853 1
Size: 346 449
Fragments arranged by size:
1325 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 644 107
Size: 537 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 628 2251 2432 2595 628
Size: 1623 181 163 2334
Fragments arranged by size:
2334 1623 181 163
BtsI GCAGTG_nn'
Cuts at: 1544 1544
Size: 4301
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DraIII CAC_nnn'GTG
Cuts at: 1270 1270
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1886 2060 2451 2478 3823 1886
Size: 174 391 27 1345 2364
Fragments arranged by size:
2364 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 487 976 2324 2534 487
Size: 489 1348 210 2254
Fragments arranged by size:
2254 1348 489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 361 672 771 2126 2558 3305 3573 307
Size: 54 311 99 1355 432 747 268 1035
Fragments arranged by size:
1355 1035 747 432 311 268 99 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1010 1010
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 1440 1440
Size: 4301
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1177 2320 1177
Size: 1143 3158
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 759 791 2143 2173 2175 2205 712
Size: 32 15 32 1352 30 2 30 2808
Fragments arranged by size:
2808 1352 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 162 720 1340 162
Size: 558 620 3123
Fragments arranged by size:
3123 620 558
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2972 3156 2972
Size: 184 4117
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 397 403 2290 2417 397
Size: 6 1887 127 2281
Fragments arranged by size:
2281 1887 127 6
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 3099 3344 1412
Size: 314 360 1013 245 2369
Fragments arranged by size:
2369 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NcoI C'CATG_G
Cuts at: 107 644 107
Size: 537 3764
NdeI CA'TA_TG
Cuts at: 747 747
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 396 1145 1354 1854 2761 396
Size: 749 209 500 907 1936
Fragments arranged by size:
1936 907 749 500 209
PasI CC'CwG_GG
Cuts at: 623 732 623
Size: 109 4192
PciI A'CATG_T
Cuts at: 392 1141 2757 392
Size: 749 1616 1936
Fragments arranged by size:
1936 1616 749
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PmlI CAC'GTG
Cuts at: 585 585
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PstI C_TGCA'G
Cuts at: 521 1348 2033 521
Size: 827 685 2789
Fragments arranged by size:
2789 827 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 487 2324 2534 487
Size: 1837 210 2254
Fragments arranged by size:
2254 1837 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 792 3818 792
Size: 3026 1275
SfcI C'TryA_G
Cuts at: 33 517 1344 2029 3022 3213 33
Size: 484 827 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 484 191
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 1045 1456 2371 2863 3125 1045
Size: 411 915 492 262 2221
Fragments arranged by size:
2221 915 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
SspI AAT'ATT
Cuts at: 561 561
Size: 4301
StyI C'CwwG_G
Cuts at: 107 644 1244 1435 1789 107
Size: 537 600 191 354 2619
Fragments arranged by size:
2619 600 537 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 1884 2017 2476 2685 409
Size: 1475 133 459 209 2025
Fragments arranged by size:
2025 1475 459 209 133
TatI w'GTAC_w
Cuts at: 225 263 790 810 3816 225
Size: 38 527 20 3006 710
Fragments arranged by size:
3006 710 527 38 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 1168 2098 1168
Size: 930 3371
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1796 663
Size: 1133 3168
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 481 1180 481
Size: 699 3602
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI