Restriction map for
pF1KE0632
(Circular) (Six-base) MAPSORT of: pF1KE0632.seq Check: 4019 from: 1 to: 4241
pF1KE0632 4241 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 1010 1010
Size: 4241
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 702 1257 702
Size: 555 3686
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 3245 3513 2066
Size: 432 747 268 2794
Fragments arranged by size:
2794 747 432 268
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1110 1149 1546 3113 1110
Size: 39 397 1567 2238
Fragments arranged by size:
2238 1567 397 39
ApaI G_GGCC'C
Cuts at: 1223 1223
Size: 4241
ApaLI G'TGCA_C
Cuts at: 256 307 494 3011 256
Size: 51 187 2517 1486
Fragments arranged by size:
2517 1486 187 51
ApoI r'AATT_y
Cuts at: 190 1245 3594 3850 190
Size: 1055 2349 256 581
Fragments arranged by size:
2349 1055 581 256
AscI GG'CGCG_CC
Cuts at: 1058 1058
Size: 4241
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 565 706 903 1053 1079 1261 565
Size: 141 197 150 26 182 3545
Fragments arranged by size:
3545 197 182 150 141 26
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 637 675 702 922 1257 1671 1919 1954 637
Size: 38 27 220 335 414 248 35 2924
Fragments arranged by size:
2924 414 335 248 220 38 35 27
BanII G_rGCy'C
Cuts at: 436 1223 1255 2285 436
Size: 787 32 1030 2392
Fragments arranged by size:
2392 1030 787 32
BbeI G_GCGC'C
Cuts at: 641 1675 1923 641
Size: 1034 248 2959
Fragments arranged by size:
2959 1034 248
BbsI GAAGACnn'nnnn_
Cuts at: 378 793 378
Size: 415 3826
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1153 1353 1153
Size: 200 4041
Bme1580I G_kGCm'C
Cuts at: 260 311 498 680 1024 1223 1866 1959 3015
Size: 51 187 182 344 199 643 93 1056
Cuts at: 3015 260
Size: 1486
Fragments arranged by size:
1486 1056 643 344 199 187 182 93
51
BmgBI CAC'GTC
Cuts at: 1039 1039
Size: 4241
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 778 1048 1126 688
Size: 90 270 78 3803
Fragments arranged by size:
3803 270 90 78
Bpu10I CC'TnA_GC
Cuts at: 379 379
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 308 3945 308
Size: 3637 604
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 638 1007 1672 1920 2622 4118 638
Size: 369 665 248 702 1496 761
Fragments arranged by size:
1496 761 702 665 369 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 256
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 1225 418
Size: 807 3434
BseYI C'CCAG_C
Cuts at: 459 615 1666 2424 3001 3497 459
Size: 156 1051 758 577 496 1203
Fragments arranged by size:
1203 1051 758 577 496 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 574 1019 462
Size: 112 445 3684
Fragments arranged by size:
3684 445 112
BsiEI CG_ry'CG
Cuts at: 104 888 1783 1829 3037 104
Size: 784 895 46 1208 1308
Fragments arranged by size:
1308 1208 895 784 46
BsiHKAI G_wGCw'C
Cuts at: 260 311 498 727 1217 1255 2033 2223 3015
Size: 51 187 229 490 38 778 190 792
Cuts at: 3015 260
Size: 1486
Fragments arranged by size:
1486 792 778 490 229 190 187 51
38
BsmBI CGTCTCn'nnnn_
Cuts at: 562 562
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 260 311 436 498 680 727 1024 1217 1223
Size: 51 125 62 182 47 297 193 6
Cuts at: 1223 1255 1866 1959 2033 2223 2285 3015 260
Size: 32 611 93 74 190 62 730 1486
Fragments arranged by size:
1486 730 611 297 193 190 182 125
93 74 62 62 51 47 32 6
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 1291 1505 1807 2188 326
Size: 965 214 302 381 2379
Fragments arranged by size:
2379 965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 575 593 2239 2420 2629 575
Size: 18 1646 181 209 2187
Fragments arranged by size:
2187 1646 209 181 18
BssHII G'CGCG_C
Cuts at: 464 1058 464
Size: 594 3647
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1110 1618 1110
Size: 508 3733
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 926 1223 926
Size: 297 3944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 647 1007 2191 2372 2535 647
Size: 360 1184 181 163 2353
Fragments arranged by size:
2353 1184 360 181 163
BtsI GCAGTG_nn'
Cuts at: 167 1484 167
Size: 1317 2924
ClaI AT'CG_AT
Cuts at: 548 548
Size: 4241
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1006 1947 2805 1006
Size: 941 858 2442
Fragments arranged by size:
2442 941 858
EaeI y'GGCC_r
Cuts at: 779 885 1826 2000 2391 2418 3763 779
Size: 106 941 174 391 27 1345 1257
Fragments arranged by size:
1345 1257 941 391 174 106 27
EagI C'GGCC_G
Cuts at: 885 1826 885
Size: 941 3300
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 202 462 517 733 1000 2449 2759 2905 4051
Size: 260 55 216 267 1449 310 146 1146
Cuts at: 4051 202
Size: 392
Fragments arranged by size:
1449 1146 392 310 267 260 216 146
55
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 778 1048 1126 2066 2498 3245 3513 688
Size: 90 270 78 940 432 747 268 1416
Fragments arranged by size:
1416 940 747 432 270 268 90 78
EcoO109I rG'GnC_Cy
Cuts at: 481 711 753 940 1119 1380 481
Size: 230 42 187 179 261 3342
Fragments arranged by size:
3342 261 230 187 179 42
EcoRI G'AATT_C
Cuts at: 190 1245 190
Size: 1055 3186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 460 641 1192 1675 1923 2945 3924 460
Size: 181 551 483 248 1022 979 777
Fragments arranged by size:
1022 979 777 551 483 248 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2083 2113 2115 2145 2083
Size: 30 2 30 4179
Fragments arranged by size:
4179 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 637 1671 1919 637
Size: 1034 248 2959
Fragments arranged by size:
2959 1034 248
KpnI G_GTAC'C
Cuts at: 706 1261 706
Size: 555 3686
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 2912 3096 299
Size: 2613 184 1444
Fragments arranged by size:
2613 1444 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 550 775 808 2230 2357 550
Size: 225 33 1422 127 2434
Fragments arranged by size:
2434 1422 225 127 33
MspA1I CmG'CkG
Cuts at: 615 928 1107 1116 1146 1225 1352 1666 2026
Size: 313 179 9 30 79 127 314 360
Cuts at: 2026 3039 3284 615
Size: 1013 245 1572
Fragments arranged by size:
1572 1013 360 314 313 245 179 127
79 30 9
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 638 1672 1920 638
Size: 1034 248 2959
Fragments arranged by size:
2959 1034 248
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NotI GC'GGCC_GC
Cuts at: 885 885
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PfoI T'CCnGG_A
Cuts at: 755 755
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 481 753 940 1119 481
Size: 272 187 179 3603
Fragments arranged by size:
3603 272 187 179
PshAI GACnn'nnGTC
Cuts at: 1175 1175
Size: 4241
PspOMI G'GGCC_C
Cuts at: 1219 1219
Size: 4241
PstI C_TGCA'G
Cuts at: 965 1115 1288 1973 965
Size: 150 173 685 3233
Fragments arranged by size:
3233 685 173 150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1666 2026 1666
Size: 360 3881
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SacII CC_GC'GG
Cuts at: 929 1226 929
Size: 297 3944
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SanDI GG'GwC_CC
Cuts at: 940 940
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SexAI A'CCwGG_T
Cuts at: 557 797 557
Size: 240 4001
SfcI C'TryA_G
Cuts at: 33 961 1111 1284 1969 2962 3153 33
Size: 928 150 173 685 993 191 1121
Fragments arranged by size:
1121 993 928 685 191 173 150
SfoI GGC'GCC
Cuts at: 639 1673 1921 639
Size: 1034 248 2959
Fragments arranged by size:
2959 1034 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SgrAI Cr'CCGG_yG
Cuts at: 575 575
Size: 4241
SmaI CCC'GGG
Cuts at: 708 1055 1081 1263 708
Size: 347 26 182 3686
Fragments arranged by size:
3686 347 182 26
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 1375 1729 1375
Size: 354 3887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1824 1957 2416 2625 277
Size: 1547 133 459 209 1893
Fragments arranged by size:
1893 1547 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 3758 3790 256
Size: 32 3470 32 707
Fragments arranged by size:
3470 707 32 32
Tth111I GACn'n_nGTC
Cuts at: 559 751 799 1009 2038 559
Size: 192 48 210 1029 2762
Fragments arranged by size:
2762 1029 210 192 48
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1736 549
Size: 1187 3054
XmaI C'CCGG_G
Cuts at: 706 1053 1079 1261 706
Size: 347 26 182 3686
Fragments arranged by size:
3686 347 182 26
ZraI GAC'GTC
Cuts at: 1008 1008
Size: 4241
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI BsrGI
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PflMI PmlI PsiI PspXI
PsrI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI