Restriction map for
pF1KE0562
(Circular) (Six-base) MAPSORT of: pF1KE0562.seq Check: 2190 from: 1 to: 4097
pF1KE0562 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 209 1135 209
Size: 926 3171
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1922 2354 3101 3369 388
Size: 1534 432 747 268 1116
Fragments arranged by size:
1534 1116 747 432 268
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 144 795 1402 2969 144
Size: 651 607 1567 1272
Fragments arranged by size:
1567 1272 651 607
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 1117 1117
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 439 1113 1527 1775 1810 439
Size: 674 414 248 35 2726
Fragments arranged by size:
2726 674 414 248 35
BanII G_rGCy'C
Cuts at: 744 908 1111 2141 744
Size: 164 203 1030 2700
Fragments arranged by size:
2700 1030 203 164
BbeI G_GCGC'C
Cuts at: 443 1531 1779 443
Size: 1088 248 2761
Fragments arranged by size:
2761 1088 248
BbsI GAAGACnn'nnnn_
Cuts at: 283 376 487 861 283
Size: 93 111 374 3519
Fragments arranged by size:
3519 374 111 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 696 730 696
Size: 34 4063
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 786 1617 786
Size: 831 3266
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 607 1209 607
Size: 602 3495
Bme1580I G_kGCm'C
Cuts at: 677 1722 1815 2871 677
Size: 1045 93 1056 1903
Fragments arranged by size:
1903 1056 1045 93
BmrI ACTGGGnnnn_n'
Cuts at: 911 1309 1390 1725 3869 911
Size: 398 81 335 2144 1139
Fragments arranged by size:
2144 1139 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 715 903 682
Size: 33 188 3876
Fragments arranged by size:
3876 188 33
Bpu10I CC'TnA_GC
Cuts at: 389 389
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1063 3801 1063
Size: 2738 1359
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 440 1528 1776 2478 3974 440
Size: 1088 248 702 1496 563
Fragments arranged by size:
1496 1088 702 563 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 491 491
Size: 4097
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 1009 441
Size: 568 3529
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 395 577 1111 1889 2079 2871 395
Size: 182 534 778 190 792 1621
Fragments arranged by size:
1621 792 778 534 190 182
Bsp1286I G_dGCh'C
Cuts at: 395 577 677 744 908 1111 1722 1815 1889
Size: 182 100 67 164 203 611 93 74
Cuts at: 1889 2079 2141 2871 395
Size: 190 62 730 1621
Fragments arranged by size:
1621 730 611 203 190 182 164 100
93 74 67 62
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 1147 1361 1663 2044 221
Size: 926 214 302 381 2274
Fragments arranged by size:
2274 926 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BsrGI T'GTAC_A
Cuts at: 818 818
Size: 4097
BssSI C'ACGA_G
Cuts at: 628 2368 2726 628
Size: 1740 358 1999
Fragments arranged by size:
1999 1740 358
BstAPI GCAn_nnn'nTGC
Cuts at: 455 672 1474 455
Size: 217 802 3078
Fragments arranged by size:
3078 802 217
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 619 619
Size: 4097
BstYI r'GATC_y
Cuts at: 1 188 533 1122 1589 1612 1947 2193 3205
Size: 187 345 589 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 589 467 449 346 345 335 246
187 86 23 12
BtgI C'CryG_G
Cuts at: 107 420 612 107
Size: 313 192 3592
Fragments arranged by size:
3592 313 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 163 955 1340 163
Size: 792 385 2920
Fragments arranged by size:
2920 792 385
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 232 509 560 1682 1856 2247 2274 3619 232
Size: 277 51 1122 174 391 27 1345 710
Fragments arranged by size:
1345 1122 710 391 277 174 51 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 829 2305 2615 2761 3907 829
Size: 1476 310 146 1146 1019
Fragments arranged by size:
1476 1146 1019 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 682 715 903 1922 2354 3101 3369 388
Size: 294 33 188 1019 432 747 268 1116
Fragments arranged by size:
1116 1019 747 432 294 268 188 33
EcoNI CCTnn'n_nnAGG
Cuts at: 312 543 312
Size: 231 3866
EcoO109I rG'GnC_Cy
Cuts at: 378 660 1236 378
Size: 282 576 3239
Fragments arranged by size:
3239 576 282
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 555 2116 555
Size: 1561 2536
FspI TGC'GCA
Cuts at: 721 1878 721
Size: 1157 2940
FspAI rTGC'GCAy
Cuts at: 721 721
Size: 4097
HaeII r_GCGC'y
Cuts at: 443 1531 1779 2801 3780 443
Size: 1088 248 1022 979 760
Fragments arranged by size:
1088 1022 979 760 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1939 1969 1971 2001 1939
Size: 30 2 30 4035
Fragments arranged by size:
4035 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
HindIII A'AGCT_T
Cuts at: 882 882
Size: 4097
KasI G'GCGC_C
Cuts at: 439 1527 1775 439
Size: 1088 248 2761
Fragments arranged by size:
2761 1088 248
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 511 562 1858 511
Size: 51 1296 2750
Fragments arranged by size:
2750 1296 51
MslI CAynn'nnrTG
Cuts at: 228 419 640 2086 2213 228
Size: 191 221 1446 127 2112
Fragments arranged by size:
2112 1446 221 191 127
MspA1I CmG'CkG
Cuts at: 1208 1522 1882 2895 3140 1208
Size: 314 360 1013 245 2165
Fragments arranged by size:
2165 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 440 1528 1776 440
Size: 1088 248 2761
Fragments arranged by size:
2761 1088 248
NcoI C'CATG_G
Cuts at: 107 420 612 107
Size: 313 192 3592
Fragments arranged by size:
3592 313 192
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 185 987 185
Size: 802 3295
PfoI T'CCnGG_A
Cuts at: 266 266
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 4097
PstI C_TGCA'G
Cuts at: 158 1144 1829 158
Size: 986 685 2426
Fragments arranged by size:
2426 986 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 154 1140 1825 2818 3009 33
Size: 121 986 685 993 191 1121
Fragments arranged by size:
1121 993 986 685 191 121
SfoI GGC'GCC
Cuts at: 441 1529 1777 441
Size: 1088 248 2761
Fragments arranged by size:
2761 1088 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StuI AGG'CCT
Cuts at: 924 924
Size: 4097
StyI C'CwwG_G
Cuts at: 107 420 612 1231 1585 107
Size: 313 192 619 354 2619
Fragments arranged by size:
2619 619 354 313 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 1680 1813 2272 2481 643
Size: 1037 133 459 209 2259
Fragments arranged by size:
2259 1037 459 209 133
TatI w'GTAC_w
Cuts at: 818 3612 818
Size: 2794 1303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 309 341 3614 3646 309
Size: 32 3273 32 760
Fragments arranged by size:
3273 760 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
XmnI GAAnn'nnTTC
Cuts at: 484 484
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI