Restriction map for
pF1KE0561
(Circular) (Six-base) MAPSORT of: pF1KE0561.seq Check: 9335 from: 1 to: 4112
pF1KE0561 4112 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 574 1150 574
Size: 576 3536
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 400 730 1937 2369 3116 3384 159
Size: 241 330 1207 432 747 268 887
Fragments arranged by size:
1207 887 747 432 330 268 241
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 719 2500 719
Size: 1781 2331
AlwNI CAG_nnn'CTG
Cuts at: 1095 1417 2984 1095
Size: 322 1567 2223
Fragments arranged by size:
2223 1567 322
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 335 756 1128 1542 1790 1825 335
Size: 421 372 414 248 35 2622
Fragments arranged by size:
2622 421 414 372 248 35
BanII G_rGCy'C
Cuts at: 440 1126 2156 440
Size: 686 1030 2396
Fragments arranged by size:
2396 1030 686
BbeI G_GCGC'C
Cuts at: 339 1546 1794 339
Size: 1207 248 2657
Fragments arranged by size:
2657 1207 248
BbsI GAAGACnn'nnnn_
Cuts at: 295 388 295
Size: 93 4019
BbvCI CC'TCA_GC
Cuts at: 296 296
Size: 4112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4078
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 801 1632 801
Size: 831 3281
BglI GCCn_nnn'nGGC
Cuts at: 475 475
Size: 4112
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 1737 1830 2886 1737
Size: 93 1056 2963
Fragments arranged by size:
2963 1056 93
BmgBI CAC'GTC
Cuts at: 895 895
Size: 4112
BmrI ACTGGGnnnn_n'
Cuts at: 538 1324 1405 1740 3884 538
Size: 786 81 335 2144 766
Fragments arranged by size:
2144 786 766 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 4112
Bpu10I CC'TnA_GC
Cuts at: 296 296
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 355 1288 2167 2659 2957 118
Size: 237 933 879 492 298 1273
Fragments arranged by size:
1273 933 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 1078 3816 1078
Size: 2738 1374
BsaAI yAC'GTr
Cuts at: 1042 1465 1042
Size: 423 3689
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 336 1543 1791 2493 3989 336
Size: 1207 248 702 1496 459
Fragments arranged by size:
1496 1207 702 459 248
BsaWI w'CCGG_w
Cuts at: 719 1301 1822 2500 2774 2921 3332 719
Size: 582 521 678 274 147 411 1499
Fragments arranged by size:
1499 678 582 521 411 274 147
BseYI C'CCAG_C
Cuts at: 400 1537 2295 2872 3368 400
Size: 1137 758 577 496 1144
Fragments arranged by size:
1144 1137 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 696
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 309 1654 1700 2908 104
Size: 205 1345 46 1208 1308
Fragments arranged by size:
1345 1308 1208 205 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 781 781
Size: 4112
Bsp1286I G_dGCh'C
Cuts at: 440 1126 1737 1830 1904 2094 2156 2886 440
Size: 686 611 93 74 190 62 730 1666
Fragments arranged by size:
1666 730 686 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 387 3288 387
Size: 2901 1211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 777 1162 1376 1678 2059 777
Size: 385 214 302 381 2830
Fragments arranged by size:
2830 385 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 311 719 2110 2291 2500 311
Size: 408 1391 181 209 1923
Fragments arranged by size:
1923 1391 408 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 756 1489 756
Size: 733 3379
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 433 433
Size: 4112
BstYI r'GATC_y
Cuts at: 1 1137 1604 1627 1962 2208 3220 3306 3318
Size: 1136 467 23 335 246 1012 86 12
Cuts at: 3318 3664 1
Size: 346 449
Fragments arranged by size:
1136 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 480 480
Size: 4112
BtgI C'CryG_G
Cuts at: 447 447
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 186 221 186
Size: 35 4077
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 306 459 1697 1871 2262 2289 3634 306
Size: 153 1238 174 391 27 1345 784
Fragments arranged by size:
1345 1238 784 391 174 153 27
EagI C'GGCC_G
Cuts at: 306 1697 306
Size: 1391 2721
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 400 730 1117 1937 2369 3116 3384 159
Size: 241 330 387 820 432 747 268 887
Fragments arranged by size:
887 820 747 432 387 330 268 241
EcoNI CCTnn'n_nnAGG
Cuts at: 299 324 299
Size: 25 4087
EcoO109I rG'GnC_Cy
Cuts at: 129 581 918 1251 129
Size: 452 337 333 2990
Fragments arranged by size:
2990 452 337 333
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 736 1893 736
Size: 1157 2955
FspAI rTGC'GCAy
Cuts at: 736 736
Size: 4112
HaeII r_GCGC'y
Cuts at: 339 1546 1794 2816 3795 339
Size: 1207 248 1022 979 656
Fragments arranged by size:
1207 1022 979 656 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 972 1004 1954 1984 1986 2016 972
Size: 32 950 30 2 30 3068
Fragments arranged by size:
3068 950 32 30 30 2
HincII GTy'rAC
Cuts at: 213 834 1151 213
Size: 621 317 3174
Fragments arranged by size:
3174 621 317
HindIII A'AGCT_T
Cuts at: 373 373
Size: 4112
KasI G'GCGC_C
Cuts at: 335 1542 1790 335
Size: 1207 248 2657
Fragments arranged by size:
2657 1207 248
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 461 1873 461
Size: 1412 2700
MslI CAynn'nnrTG
Cuts at: 431 652 2101 2228 431
Size: 221 1449 127 2315
Fragments arranged by size:
2315 1449 221 127
MspA1I CmG'CkG
Cuts at: 1223 1537 1897 2910 3155 1223
Size: 314 360 1013 245 2180
Fragments arranged by size:
2180 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 336 1543 1791 336
Size: 1207 248 2657
Fragments arranged by size:
2657 1207 248
NcoI C'CATG_G
Cuts at: 447 447
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NotI GC'GGCC_GC
Cuts at: 306 306
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 861 1095 861
Size: 234 3878
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 129 129
Size: 4112
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SexAI A'CCwGG_T
Cuts at: 221 221
Size: 4112
SfcI C'TryA_G
Cuts at: 33 351 575 930 1155 1840 2833 3024 33
Size: 318 224 355 225 685 993 191 1121
Fragments arranged by size:
1121 993 685 355 318 225 224 191
SfoI GGC'GCC
Cuts at: 337 1544 1792 337
Size: 1207 248 2657
Fragments arranged by size:
2657 1207 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 133 370 1267 2182 2674 2936 133
Size: 237 897 915 492 262 1309
Fragments arranged by size:
1309 915 897 492 262 237
SnaBI TAC'GTA
Cuts at: 1042 1042
Size: 4112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 478 478
Size: 4112
StyI C'CwwG_G
Cuts at: 447 1246 1600 447
Size: 799 354 2959
Fragments arranged by size:
2959 799 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 454 1607 454
Size: 1153 2959
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 850 850
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BseRI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI