Restriction map for
pF1KE0546
(Circular) (Six-base) MAPSORT of: pF1KE0546.seq Check: 4680 from: 1 to: 4004
pF1KE0546 4004 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 597 597
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1829 2261 3008 3276 532
Size: 1297 432 747 268 1260
Fragments arranged by size:
1297 1260 747 432 268
AflIII A'CryG_T
Cuts at: 206 1356 2460 3437 206
Size: 1150 1104 977 773
Fragments arranged by size:
1150 1104 977 773
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AhdI GACnn_n'nnGTC
Cuts at: 309 309
Size: 4004
AleI CACnn'nnGTG
Cuts at: 391 391
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 612 1309 2876 612
Size: 697 1567 1740
Fragments arranged by size:
1740 1567 697
ApaI G_GGCC'C
Cuts at: 188 784 957 188
Size: 596 173 3235
Fragments arranged by size:
3235 596 173
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 230 253 437 711 1024 230
Size: 23 184 274 313 3210
Fragments arranged by size:
3210 313 274 184 23
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 226 271 676 727 1020 1434 1682 1717 226
Size: 45 405 51 293 414 248 35 2513
Fragments arranged by size:
2513 414 405 293 248 51 45 35
BanII G_rGCy'C
Cuts at: 188 542 784 794 957 1018 2048 188
Size: 354 242 10 163 61 1030 2144
Fragments arranged by size:
2144 1030 354 242 163 61 10
BbeI G_GCGC'C
Cuts at: 275 1438 1686 275
Size: 1163 248 2593
Fragments arranged by size:
2593 1163 248
BbsI GAAGACnn'nnnn_
Cuts at: 555 555
Size: 4004
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglI GCCn_nnn'nGGC
Cuts at: 371 371
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 188 679 784 957 1629 1722 2778 188
Size: 491 105 173 672 93 1056 1414
Fragments arranged by size:
1414 1056 672 491 173 105 93
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1297 1632 3776 1216
Size: 81 335 2144 1444
Fragments arranged by size:
2144 1444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 644 1180 2059 2551 2849 577
Size: 67 536 879 492 298 1732
Fragments arranged by size:
1732 879 536 492 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 144 662 3708 144
Size: 518 3046 440
Fragments arranged by size:
3046 518 440
BsaAI yAC'GTr
Cuts at: 264 393 848 876 1357 264
Size: 129 455 28 481 2911
Fragments arranged by size:
2911 481 455 129 28
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 272 310 1435 1683 2385 3881 272
Size: 38 1125 248 702 1496 395
Fragments arranged by size:
1496 1125 702 395 248 38
BsaWI w'CCGG_w
Cuts at: 500 1193 1714 2392 2666 2813 3224 500
Size: 693 521 678 274 147 411 1280
Fragments arranged by size:
1280 693 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 913
Size: 30 3974
BseYI C'CCAG_C
Cuts at: 180 334 1429 2187 2764 3260 180
Size: 154 1095 758 577 496 924
Fragments arranged by size:
1095 924 758 577 496 154
BsiEI CG_ry'CG
Cuts at: 104 560 1546 1592 2800 104
Size: 456 986 46 1208 1308
Fragments arranged by size:
1308 1208 986 456 46
BsiHKAI G_wGCw'C
Cuts at: 125 488 794 801 1018 1796 1986 2778 125
Size: 363 306 7 217 778 190 792 1351
Fragments arranged by size:
1351 792 778 363 306 217 190 7
Bsp1286I G_dGCh'C
Cuts at: 125 188 488 542 679 784 794 801 957
Size: 63 300 54 137 105 10 7 156
Cuts at: 957 1018 1629 1722 1796 1986 2048 2778 125
Size: 61 611 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 300 190 156 137 105
93 74 63 62 61 54 10 7
BspEI T'CCGG_A
Cuts at: 500 3224 500
Size: 2724 1280
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 597 1054 1268 1570 1951 597
Size: 457 214 302 381 2650
Fragments arranged by size:
2650 457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 584 2296 2350 3834 3999 50
Size: 534 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 534 165 55 54
BsrDI GCAATG_nn'
Cuts at: 839 1916 839
Size: 1077 2927
BsrFI r'CCGG_y
Cuts at: 555 2002 2183 2392 555
Size: 1447 181 209 2167
Fragments arranged by size:
2167 1447 209 181
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 153 724 819 153
Size: 571 95 3338
Fragments arranged by size:
3338 571 95
BstYI r'GATC_y
Cuts at: 1 822 1029 1496 1519 1854 2100 3112 3198
Size: 821 207 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 821 467 449 346 335 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 107 723 107
Size: 616 3388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DraIII CAC_nnn'GTG
Cuts at: 149 396 528 149
Size: 247 132 3625
Fragments arranged by size:
3625 247 132
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 465 557 914 1589 1763 2154 2181 3526 465
Size: 92 357 675 174 391 27 1345 943
Fragments arranged by size:
1345 943 675 391 357 174 92 27
EagI C'GGCC_G
Cuts at: 557 1589 557
Size: 1032 2972
EarI CTCTTCn'nnn_
Cuts at: 287 782 2027 2237 287
Size: 495 1245 210 2054
Fragments arranged by size:
2054 1245 495 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 2212 2522 2668 3814 172
Size: 2040 310 146 1146 362
Fragments arranged by size:
2040 1146 362 310 146
EcoICRI GAG'CTC
Cuts at: 792 1016 792
Size: 224 3780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 634 1829 2261 3008 3276 532
Size: 102 1195 432 747 268 1260
Fragments arranged by size:
1260 1195 747 432 268 102
EcoNI CCTnn'n_nnAGG
Cuts at: 760 760
Size: 4004
EcoO109I rG'GnC_Cy
Cuts at: 184 780 954 1143 184
Size: 596 174 189 3045
Fragments arranged by size:
3045 596 189 174
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 136 261 275 1438 1686 2708 3687 136
Size: 125 14 1163 248 1022 979 453
Fragments arranged by size:
1163 1022 979 453 248 125 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 640 672 1846 1876 1878 1908 295
Size: 32 313 32 1174 30 2 30 2391
Fragments arranged by size:
2391 1174 313 32 32 30 30 2
HincII GTy'rAC
Cuts at: 735 1043 735
Size: 308 3696
KasI G'GCGC_C
Cuts at: 271 1434 1682 271
Size: 1163 248 2593
Fragments arranged by size:
2593 1163 248
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 206 3437 206
Size: 3231 773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 467 1765 467
Size: 1298 2706
MslI CAynn'nnrTG
Cuts at: 391 722 1993 2120 391
Size: 331 1271 127 2275
Fragments arranged by size:
2275 1271 331 127
MspA1I CmG'CkG
Cuts at: 642 1115 1429 1789 2802 3047 642
Size: 473 314 360 1013 245 1599
Fragments arranged by size:
1599 1013 473 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 272 1435 1683 272
Size: 1163 248 2593
Fragments arranged by size:
2593 1163 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 741 1057 1557 2464 741
Size: 316 500 907 2281
Fragments arranged by size:
2281 907 500 316
PasI CC'CwG_GG
Cuts at: 978 978
Size: 4004
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 149 645 149
Size: 496 3508
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PmlI CAC'GTG
Cuts at: 393 848 876 393
Size: 455 28 3521
Fragments arranged by size:
3521 455 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PspOMI G'GGCC_C
Cuts at: 184 780 953 184
Size: 596 173 3235
Fragments arranged by size:
3235 596 173
PspXI vC'TCGA_Gb
Cuts at: 253 253
Size: 4004
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 642 1429 1789 642
Size: 787 360 2857
Fragments arranged by size:
2857 787 360
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 794 1018 794
Size: 224 3780
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 782 2027 2237 782
Size: 1245 210 2549
Fragments arranged by size:
2549 1245 210
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 1047 1732 2725 2916 33
Size: 1014 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 191
SfoI GGC'GCC
Cuts at: 273 1436 1684 273
Size: 1163 248 2593
Fragments arranged by size:
2593 1163 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 439 1026 439
Size: 587 3417
SmlI C'TyrA_G
Cuts at: 253 592 659 1159 2074 2566 2828 253
Size: 339 67 500 915 492 262 1429
Fragments arranged by size:
1429 915 500 492 339 262 67
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 107 881 1138 1492 107
Size: 774 257 354 2619
Fragments arranged by size:
2619 774 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 441 473 3521 3553 441
Size: 32 3048 32 892
Fragments arranged by size:
3048 892 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1499 150
Size: 1349 2655
XhoI C'TCGA_G
Cuts at: 253 253
Size: 4004
XmaI C'CCGG_G
Cuts at: 437 1024 437
Size: 587 3417
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PpuMI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI