Restriction map for
pF1KE0545
(Circular) (Six-base) MAPSORT of: pF1KE0545.seq Check: 1040 from: 1 to: 4244
pF1KE0545 4244 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 177 177
Size: 4244
AccI GT'mk_AC
Cuts at: 608 1282 608
Size: 674 3570
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 3248 3516 2069
Size: 432 747 268 2797
Fragments arranged by size:
2797 747 432 268
AfeI AGC'GCT
Cuts at: 998 998
Size: 4244
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 325 162
Size: 163 4081
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1201 1233 1201
Size: 32 4212
AlwNI CAG_nnn'CTG
Cuts at: 1549 3116 1549
Size: 1567 2677
ApaLI G'TGCA_C
Cuts at: 673 3014 673
Size: 2341 1903
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 230 382 1264 230
Size: 152 882 3210
Fragments arranged by size:
3210 882 152
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 274 346 1260 1674 1922 1957 274
Size: 72 914 414 248 35 2561
Fragments arranged by size:
2561 914 414 248 72 35
BanII G_rGCy'C
Cuts at: 229 1258 2288 229
Size: 1029 1030 2185
Fragments arranged by size:
2185 1030 1029
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 917 917
Size: 4244
BbvCI CC'TCA_GC
Cuts at: 718 718
Size: 4244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 683 1764 683
Size: 1081 3163
BglI GCCn_nnn'nGGC
Cuts at: 520 520
Size: 4244
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1070 1356 1070
Size: 286 3958
Bme1580I G_kGCm'C
Cuts at: 277 677 1869 1962 3018 277
Size: 400 1192 93 1056 1503
Fragments arranged by size:
1503 1192 1056 400 93
BmrI ACTGGGnnnn_n'
Cuts at: 771 1456 1537 1872 4016 771
Size: 685 81 335 2144 999
Fragments arranged by size:
2144 999 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 906 553
Size: 353 3891
Bpu10I CC'TnA_GC
Cuts at: 248 718 248
Size: 470 3774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 838 1420 2299 2791 3089 580
Size: 258 582 879 492 298 1735
Fragments arranged by size:
1735 879 582 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 397 3948 397
Size: 3551 693
BsaAI yAC'GTr
Cuts at: 285 1597 285
Size: 1312 2932
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 374 509 1675 1923 2625 4121 374
Size: 135 1166 248 702 1496 497
Fragments arranged by size:
1496 1166 702 497 248 135
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1201 1231 1201
Size: 30 4214
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 415 1209 271
Size: 144 794 3306
Fragments arranged by size:
3306 794 144
BseYI C'CCAG_C
Cuts at: 177 1669 2427 3004 3500 177
Size: 1492 758 577 496 921
Fragments arranged by size:
1492 921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 820
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 663 1786 1832 3040 104
Size: 559 1123 46 1208 1308
Fragments arranged by size:
1308 1208 1123 559 46
BsiHKAI G_wGCw'C
Cuts at: 572 677 1258 2036 2226 3018 572
Size: 105 581 778 190 792 1798
Fragments arranged by size:
1798 792 778 581 190 105
Bsp1286I G_dGCh'C
Cuts at: 229 277 572 677 1258 1869 1962 2036 2226
Size: 48 295 105 581 611 93 74 190
Cuts at: 2226 2288 3018 229
Size: 62 730 1455
Fragments arranged by size:
1455 730 611 581 295 190 105 93
74 62 48
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 1294 1508 1810 2191 177
Size: 1117 214 302 381 2230
Fragments arranged by size:
2230 1117 381 302 214
BsrBI CCG'CTC
Cuts at: 50 315 957 2536 2590 4074 4239 50
Size: 265 642 1579 54 1484 165 55
Fragments arranged by size:
1579 1484 642 265 165 55 54
BsrDI GCAATG_nn'
Cuts at: 701 2156 701
Size: 1455 2789
BsrFI r'CCGG_y
Cuts at: 142 558 2242 2423 2632 142
Size: 416 1684 181 209 1754
Fragments arranged by size:
1754 1684 416 209 181
BssHII G'CGCG_C
Cuts at: 360 360
Size: 4244
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 831 1621 831
Size: 790 3454
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 285 1024 285
Size: 739 3505
BstYI r'GATC_y
Cuts at: 1 1269 1736 1759 2094 2340 3352 3438 3450
Size: 1268 467 23 335 246 1012 86 12
Cuts at: 3450 3796 1
Size: 346 449
Fragments arranged by size:
1268 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 962 962
Size: 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 864 2194 2375 2538 864
Size: 1330 181 163 2570
Fragments arranged by size:
2570 1330 181 163
BtsI GCAGTG_nn'
Cuts at: 348 1487 348
Size: 1139 3105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 912 947 912
Size: 35 4209
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DraIII CAC_nnn'GTG
Cuts at: 746 746
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1126 1950 2808 1126
Size: 824 858 2562
Fragments arranged by size:
2562 858 824
EaeI y'GGCC_r
Cuts at: 428 456 660 1014 1829 2003 2394 2421 3766
Size: 28 204 354 815 174 391 27 1345
Cuts at: 3766 428
Size: 906
Fragments arranged by size:
1345 906 815 391 354 204 174 28
27
EagI C'GGCC_G
Cuts at: 660 1829 660
Size: 1169 3075
EarI CTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 2762 2908 4054 2452
Size: 310 146 1146 2642
Fragments arranged by size:
2642 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 906 2069 2501 3248 3516 553
Size: 353 1163 432 747 268 1281
Fragments arranged by size:
1281 1163 747 432 353 268
EcoO109I rG'GnC_Cy
Cuts at: 582 1383 582
Size: 801 3443
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 849 1000 1678 1926 2948 3927 849
Size: 151 678 248 1022 979 1166
Fragments arranged by size:
1166 1022 979 678 248 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2116 2118 2148 2086
Size: 30 2 30 4182
Fragments arranged by size:
4182 30 30 2
HincII GTy'rAC
Cuts at: 987 1283 987
Size: 296 3948
HpaI GTT'AAC
Cuts at: 987 987
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 430 1016 2005 430
Size: 586 989 2669
Fragments arranged by size:
2669 989 586
MslI CAynn'nnrTG
Cuts at: 322 355 763 2233 2360 322
Size: 33 408 1470 127 2206
Fragments arranged by size:
2206 1470 408 127 33
MspA1I CmG'CkG
Cuts at: 177 828 1146 1355 1669 2029 3042 3287 177
Size: 651 318 209 314 360 1013 245 1134
Fragments arranged by size:
1134 1013 651 360 318 314 245 209
NaeI GCC'GGC
Cuts at: 144 560 2425 144
Size: 416 1865 1963
Fragments arranged by size:
1963 1865 416
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NgoMIV G'CCGG_C
Cuts at: 142 558 2423 142
Size: 416 1865 1963
Fragments arranged by size:
1963 1865 416
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PasI CC'CwG_GG
Cuts at: 1024 1024
Size: 4244
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 783 783
Size: 4244
PfoI T'CCnGG_A
Cuts at: 530 530
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PmlI CAC'GTG
Cuts at: 285 285
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1201 1233 3383 3415 1201
Size: 32 2150 32 2030
Fragments arranged by size:
2150 2030 32 32
PpuMI rG'GwC_Cy
Cuts at: 582 582
Size: 4244
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 177 828 1669 2029 177
Size: 651 841 360 2392
Fragments arranged by size:
2392 841 651 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SexAI A'CCwGG_T
Cuts at: 968 968
Size: 4244
SfcI C'TryA_G
Cuts at: 33 1287 1972 2965 3156 33
Size: 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 232 1266 232
Size: 1034 3210
SmlI C'TyrA_G
Cuts at: 595 853 1399 2314 2806 3068 595
Size: 258 546 915 492 262 1771
Fragments arranged by size:
1771 915 546 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StuI AGG'CCT
Cuts at: 442 523 442
Size: 81 4163
StyI C'CwwG_G
Cuts at: 1378 1732 1378
Size: 354 3890
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 594 1827 1960 2419 2628 316
Size: 278 1233 133 459 209 1932
Fragments arranged by size:
1932 1233 459 278 209 133
TatI w'GTAC_w
Cuts at: 815 3759 815
Size: 2944 1300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 173 205 3761 3793 173
Size: 32 3556 32 624
Fragments arranged by size:
3556 624 32 32
Tth111I GACn'n_nGTC
Cuts at: 895 970 2041 895
Size: 75 1071 3098
Fragments arranged by size:
3098 1071 75
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1739 1739
Size: 4244
XmaI C'CCGG_G
Cuts at: 230 1264 230
Size: 1034 3210
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI ApaI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI