Restriction map for
pF1KE0540
(Circular) (Six-base) MAPSORT of: pF1KE0540.seq Check: 8302 from: 1 to: 3911
pF1KE0540 3911 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 431 534 431
Size: 103 3808
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2168 2915 3183 1736
Size: 432 747 268 2464
Fragments arranged by size:
2464 747 432 268
AflIII A'CryG_T
Cuts at: 1263 2367 3344 1263
Size: 1104 977 1830
Fragments arranged by size:
1830 1104 977
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 251 251
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 198 571 1216 2783 198
Size: 373 645 1567 1326
Fragments arranged by size:
1567 1326 645 373
ApaI G_GGCC'C
Cuts at: 235 271 235
Size: 36 3875
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 915 3264 3520 915
Size: 2349 256 1306
Fragments arranged by size:
2349 1306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 613 931 613
Size: 318 3593
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 927 1341 1589 1624 927
Size: 414 248 35 3214
Fragments arranged by size:
3214 414 248 35
BanII G_rGCy'C
Cuts at: 130 235 271 447 925 1955 130
Size: 105 36 176 478 1030 2086
Fragments arranged by size:
2086 1030 478 176 105 36
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BbsI GAAGACnn'nnnn_
Cuts at: 588 588
Size: 3911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3877
BciVI GTATCCnnnnn_n'
Cuts at: 1809 2570 3911 1809
Size: 761 1341 1809
Fragments arranged by size:
1809 1341 761
BclI T'GATC_A
Cuts at: 293 1431 293
Size: 1138 2773
BglI GCCn_nnn'nGGC
Cuts at: 334 343 334
Size: 9 3902
BglII A'GATC_T
Cuts at: 854 1426 3463 854
Size: 572 2037 1302
Fragments arranged by size:
2037 1302 572
BlpI GC'TnA_GC
Cuts at: 848 1023 848
Size: 175 3736
Bme1580I G_kGCm'C
Cuts at: 235 271 1536 1629 2685 235
Size: 36 1265 93 1056 1461
Fragments arranged by size:
1461 1265 1056 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1204 1539 3683 1123
Size: 81 335 2144 1351
Fragments arranged by size:
2144 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 2278 873
Size: 1405 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 566 550
Size: 16 3895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1087 1966 2458 2756 142
Size: 945 879 492 298 1297
Fragments arranged by size:
1297 945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3615 3615
Size: 3911
BsaAI yAC'GTr
Cuts at: 1264 1264
Size: 3911
BsaBI GATnn'nnATC
Cuts at: 6 1425 3462 6
Size: 1419 2037 455
Fragments arranged by size:
2037 1419 455
BsaHI Gr'CG_yC
Cuts at: 1342 1590 2292 3788 1342
Size: 248 702 1496 1465
Fragments arranged by size:
1496 1465 702 248
BsaWI w'CCGG_w
Cuts at: 165 1100 1621 2299 2573 2720 3131 165
Size: 935 521 678 274 147 411 945
Fragments arranged by size:
945 935 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1336 2094 2671 3167 1336
Size: 758 577 496 2080
Fragments arranged by size:
2080 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 607 562
Size: 45 3866
BsiEI CG_ry'CG
Cuts at: 104 834 1453 1499 2707 104
Size: 730 619 46 1208 1308
Fragments arranged by size:
1308 1208 730 619 46
BsiHKAI G_wGCw'C
Cuts at: 925 1703 1893 2685 925
Size: 778 190 792 2151
Fragments arranged by size:
2151 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 130 235 271 447 925 1536 1629 1703 1893
Size: 105 36 176 478 611 93 74 190
Cuts at: 1893 1955 2685 130
Size: 62 730 1356
Fragments arranged by size:
1356 730 611 478 190 176 105 93
74 62 36
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 3087 3087
Size: 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 534 803 961 1175 1477 1858 431
Size: 103 269 158 214 302 381 2484
Fragments arranged by size:
2484 381 302 269 214 158 103
BsrBI CCG'CTC
Cuts at: 50 618 2203 2257 3741 3906 50
Size: 568 1585 54 1484 165 55
Fragments arranged by size:
1585 1484 568 165 55 54
BsrDI GCAATG_nn'
Cuts at: 782 1823 782
Size: 1041 2870
BsrFI r'CCGG_y
Cuts at: 682 739 1909 2090 2299 682
Size: 57 1170 181 209 2294
Fragments arranged by size:
2294 1170 209 181 57
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 549 1288 549
Size: 739 3172
BstBI TT'CG_AA
Cuts at: 279 2272 279
Size: 1993 1918
BstXI CCAn_nnnn'nTGG
Cuts at: 271 271
Size: 3911
BstYI r'GATC_y
Cuts at: 1 854 936 1403 1426 1761 2007 3019 3105
Size: 853 82 467 23 335 246 1012 86
Cuts at: 3105 3117 3463 1
Size: 12 346 449
Fragments arranged by size:
1012 853 467 449 346 335 246 86
82 23 12
Bsu36I CC'TnA_GG
Cuts at: 340 340
Size: 3911
BtgI C'CryG_G
Cuts at: 635 635
Size: 3911
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 2042 2205 1861
Size: 181 163 3567
Fragments arranged by size:
3567 181 163
BtsI GCAGTG_nn'
Cuts at: 501 900 1154 501
Size: 399 254 3258
Fragments arranged by size:
3258 399 254
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 674 1617 2475 674
Size: 943 858 2110
Fragments arranged by size:
2110 943 858
EaeI y'GGCC_r
Cuts at: 831 1496 1670 2061 2088 3433 831
Size: 665 174 391 27 1345 1309
Fragments arranged by size:
1345 1309 665 391 174 27
EagI C'GGCC_G
Cuts at: 831 1496 831
Size: 665 3246
EarI CTCTTCn'nnn_
Cuts at: 820 1934 2144 820
Size: 1114 210 2587
Fragments arranged by size:
2587 1114 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2119 2429 2575 3721 2119
Size: 310 146 1146 2309
Fragments arranged by size:
2309 1146 310 146
EcoICRI GAG'CTC
Cuts at: 923 923
Size: 3911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 566 1736 2168 2915 3183 550
Size: 16 1170 432 747 268 1278
Fragments arranged by size:
1278 1170 747 432 268 16
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 3911
EcoO109I rG'GnC_Cy
Cuts at: 179 195 209 231 232 267 268 326 1050
Size: 16 14 22 1 35 1 58 724
Cuts at: 1050 179
Size: 3040
Fragments arranged by size:
3040 724 58 35 22 16 14 1
1
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 1930 477
Size: 1453 2458
FspI TGC'GCA
Cuts at: 1692 1692
Size: 3911
HaeII r_GCGC'y
Cuts at: 1345 1593 2615 3594 1345
Size: 248 1022 979 1662
Fragments arranged by size:
1662 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1753 1783 1785 1815 1753
Size: 30 2 30 3849
Fragments arranged by size:
3849 30 30 2
HincII GTy'rAC
Cuts at: 950 950
Size: 3911
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2582 2766 2582
Size: 184 3727
MscI TGG'CCA
Cuts at: 1672 1672
Size: 3911
MslI CAynn'nnrTG
Cuts at: 604 1900 2027 604
Size: 1296 127 2488
Fragments arranged by size:
2488 1296 127
MspA1I CmG'CkG
Cuts at: 400 558 1022 1336 1696 2709 2954 400
Size: 158 464 314 360 1013 245 1357
Fragments arranged by size:
1357 1013 464 360 314 245 158
NaeI GCC'GGC
Cuts at: 741 2092 741
Size: 1351 2560
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NcoI C'CATG_G
Cuts at: 635 635
Size: 3911
NgoMIV G'CCGG_C
Cuts at: 739 2090 739
Size: 1351 2560
NspI r_CATG'y
Cuts at: 964 1464 2371 964
Size: 500 907 2504
Fragments arranged by size:
2504 907 500
PasI CC'CwG_GG
Cuts at: 175 183 264 271 175
Size: 8 81 7 3815
Fragments arranged by size:
3815 81 8 7
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PflMI CCAn_nnn'nTGG
Cuts at: 137 426 137
Size: 289 3622
PfoI T'CCnGG_A
Cuts at: 650 650
Size: 3911
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PpuMI rG'GwC_Cy
Cuts at: 179 209 179
Size: 30 3881
PspOMI G'GGCC_C
Cuts at: 231 267 231
Size: 36 3875
PstI C_TGCA'G
Cuts at: 240 365 958 1643 240
Size: 125 593 685 2508
Fragments arranged by size:
2508 685 593 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 400 558 1336 1696 400
Size: 158 778 360 2615
Fragments arranged by size:
2615 778 360 158
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 925 925
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SanDI GG'GwC_CC
Cuts at: 179 179
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 3428 3428
Size: 3911
SfcI C'TryA_G
Cuts at: 33 236 361 954 1639 2632 2823 33
Size: 203 125 593 685 993 191 1121
Fragments arranged by size:
1121 993 685 593 203 191 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 334 334
Size: 3911
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 157 1066 1981 2473 2735 157
Size: 909 915 492 262 1333
Fragments arranged by size:
1333 915 909 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 964 1464 964
Size: 500 3411
StuI AGG'CCT
Cuts at: 302 302
Size: 3911
StyI C'CwwG_G
Cuts at: 635 746 1045 1399 635
Size: 111 299 354 3147
Fragments arranged by size:
3147 354 299 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 465 677 1494 1627 2086 2295 465
Size: 212 817 133 459 209 2081
Fragments arranged by size:
2081 817 459 212 209 133
TatI w'GTAC_w
Cuts at: 485 728 3426 485
Size: 243 2698 970
Fragments arranged by size:
2698 970 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 1708 1708
Size: 3911
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1406 1406
Size: 3911
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI