Restriction map for
pF1KE0529
(Circular) (Six-base) MAPSORT of: pF1KE0529.seq Check: 9454 from: 1 to: 3755
pF1KE0529 3755 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 248 614 771 248
Size: 366 157 3232
Fragments arranged by size:
3232 366 157
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1580 2012 2759 3027 1580
Size: 432 747 268 2308
Fragments arranged by size:
2308 747 432 268
AflII C'TTAA_G
Cuts at: 715 715
Size: 3755
AflIII A'CryG_T
Cuts at: 113 1107 2211 3188 113
Size: 994 1104 977 680
Fragments arranged by size:
1104 994 977 680
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 222 1060 2627 222
Size: 838 1567 1350
Fragments arranged by size:
1567 1350 838
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 349 627 759 3108 3364 349
Size: 278 132 2349 256 740
Fragments arranged by size:
2349 740 278 256 132
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 775 775
Size: 3755
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 248 614 771 1185 1433 1468 248
Size: 366 157 414 248 35 2535
Fragments arranged by size:
2535 414 366 248 157 35
BanII G_rGCy'C
Cuts at: 769 1799 769
Size: 1030 2725
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BbsI GAAGACnn'nnnn_
Cuts at: 228 727 228
Size: 499 3256
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 518 1275 518
Size: 757 2998
BglII A'GATC_T
Cuts at: 173 1270 3307 173
Size: 1097 2037 621
Fragments arranged by size:
2037 1097 621
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 588 1380 1473 2529 588
Size: 792 93 1056 1814
Fragments arranged by size:
1814 1056 792 93
BmrI ACTGGGnnnn_n'
Cuts at: 403 967 1048 1383 3527 403
Size: 564 81 335 2144 631
Fragments arranged by size:
2144 631 564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 412 475 551 677 198
Size: 214 63 76 126 3276
Fragments arranged by size:
3276 214 126 76 63
Bpu10I CC'TnA_GC
Cuts at: 136 136
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1810 2302 2600 931
Size: 879 492 298 2086
Fragments arranged by size:
2086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 535 3459 535
Size: 2924 831
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 1186 1434 2136 3632 1186
Size: 248 702 1496 1309
Fragments arranged by size:
1496 1309 702 248
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 259
Size: 30 3725
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 301
Size: 3755
BseYI C'CCAG_C
Cuts at: 165 506 1180 1938 2515 3011 165
Size: 341 674 758 577 496 909
Fragments arranged by size:
909 758 674 577 496 341
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 769 1547 1737 2529 769
Size: 778 190 792 1995
Fragments arranged by size:
1995 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 588 769 1380 1473 1547 1737 1799 2529 588
Size: 181 611 93 74 190 62 730 1814
Fragments arranged by size:
1814 730 611 190 181 93 74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 515 2931 515
Size: 2416 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 1753 1934 2143 1753
Size: 181 209 3365
Fragments arranged by size:
3365 209 181
BsrGI T'GTAC_A
Cuts at: 332 332
Size: 3755
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstXI CCAn_nnnn'nTGG
Cuts at: 252 252
Size: 3755
BstYI r'GATC_y
Cuts at: 1 173 218 458 780 1247 1270 1605 1851
Size: 172 45 240 322 467 23 335 246
Cuts at: 1851 2863 2949 2961 3307 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 322 246 240
172 86 45 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 149 1340 1514 1905 1932 3277 149
Size: 1191 174 391 27 1345 627
Fragments arranged by size:
1345 1191 627 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 205 517 1778 1988 205
Size: 312 1261 210 1972
Fragments arranged by size:
1972 1261 312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 376 1963 2273 2419 3565 376
Size: 1587 310 146 1146 566
Fragments arranged by size:
1587 1146 566 310 146
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 412 475 551 677 1580 2012 2759 3027
Size: 214 63 76 126 903 432 747 268
Cuts at: 3027 198
Size: 926
Fragments arranged by size:
926 903 747 432 268 214 126 76
63
EcoO109I rG'GnC_Cy
Cuts at: 894 894
Size: 3755
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3755
FspI TGC'GCA
Cuts at: 1536 1536
Size: 3755
HaeII r_GCGC'y
Cuts at: 1189 1437 2459 3438 1189
Size: 248 1022 979 1506
Fragments arranged by size:
1506 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1597 1627 1629 1659 1597
Size: 30 2 30 3693
Fragments arranged by size:
3693 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 252 618 775 252
Size: 366 157 3232
Fragments arranged by size:
3232 366 157
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 151 1516 151
Size: 1365 2390
MslI CAynn'nnrTG
Cuts at: 112 292 1744 1871 112
Size: 180 1452 127 1996
Fragments arranged by size:
1996 1452 180 127
MspA1I CmG'CkG
Cuts at: 866 1180 1540 2553 2798 866
Size: 314 360 1013 245 1823
Fragments arranged by size:
1823 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 808 1308 2215 808
Size: 500 907 2348
Fragments arranged by size:
2348 907 500
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PflMI CCAn_nnn'nTGG
Cuts at: 147 415 147
Size: 268 3487
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PshAI GACnn'nnGTC
Cuts at: 709 709
Size: 3755
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 1180 1540 1180
Size: 360 3395
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 205 517 1778 1988 205
Size: 312 1261 210 1972
Fragments arranged by size:
1972 1261 312 210
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SexAI A'CCwGG_T
Cuts at: 244 244
Size: 3755
SfcI C'TryA_G
Cuts at: 33 438 798 1483 2476 2667 33
Size: 405 360 685 993 191 1121
Fragments arranged by size:
1121 993 685 405 360 191
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 715 910 1825 2317 2579 715
Size: 195 915 492 262 1891
Fragments arranged by size:
1891 915 492 262 195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StyI C'CwwG_G
Cuts at: 889 1243 889
Size: 354 3401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 332 663 3270 332
Size: 331 2607 817
Fragments arranged by size:
2607 817 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1250 249
Size: 1001 2754
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI