Restriction map for
pF1KE0526
(Circular) (Six-base) MAPSORT of: pF1KE0526.seq Check: 1626 from: 1 to: 3644
pF1KE0526 3644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 535 535
Size: 3644
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1469 1901 2648 2916 616
Size: 853 432 747 268 1344
Fragments arranged by size:
1344 853 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AlwNI CAG_nnn'CTG
Cuts at: 209 228 587 949 2516 209
Size: 19 359 362 1567 1337
Fragments arranged by size:
1567 1337 362 359 19
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 648 2997 3253 648
Size: 2349 256 1039
Fragments arranged by size:
2349 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 332 463 664 332
Size: 131 201 3312
Fragments arranged by size:
3312 201 131
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 660 1074 1322 1357 660
Size: 414 248 35 2947
Fragments arranged by size:
2947 414 248 35
BanII G_rGCy'C
Cuts at: 265 658 1688 265
Size: 393 1030 2221
Fragments arranged by size:
2221 1030 393
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BbvCI CC'TCA_GC
Cuts at: 527 527
Size: 3644
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglII A'GATC_T
Cuts at: 287 617 1159 3196 287
Size: 330 542 2037 735
Fragments arranged by size:
2037 735 542 330
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 1269 1362 2418 1269
Size: 93 1056 2495
Fragments arranged by size:
2495 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 369 856 937 1272 3416 369
Size: 487 81 335 2144 597
Fragments arranged by size:
2144 597 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 284 2011 284
Size: 1727 1917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 336 140
Size: 196 3448
Bpu10I CC'TnA_GC
Cuts at: 527 551 527
Size: 24 3620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 1158 3195 6
Size: 1152 2037 455
Fragments arranged by size:
2037 1152 455
BsaHI Gr'CG_yC
Cuts at: 1075 1323 2025 3521 1075
Size: 248 702 1496 1198
Fragments arranged by size:
1496 1198 702 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 264 1069 1827 2404 2900 264
Size: 805 758 577 496 1008
Fragments arranged by size:
1008 805 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 470
Size: 3644
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 552 658 1436 1626 2418 552
Size: 106 778 190 792 1778
Fragments arranged by size:
1778 792 778 190 106
Bsp1286I G_dGCh'C
Cuts at: 265 552 658 1269 1362 1436 1626 1688 2418
Size: 287 106 611 93 74 190 62 730
Cuts at: 2418 265
Size: 1491
Fragments arranged by size:
1491 730 611 287 190 106 93 74
62
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 694 908 1210 1591 535
Size: 159 214 302 381 2588
Fragments arranged by size:
2588 381 302 214 159
BsrBI CCG'CTC
Cuts at: 50 504 1936 1990 3474 3639 50
Size: 454 1432 54 1484 165 55
Fragments arranged by size:
1484 1432 454 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 1642 1823 2032 1642
Size: 181 209 3254
Fragments arranged by size:
3254 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 1021
Size: 3644
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 206 214 287 617 669 1136 1159 1494
Size: 205 8 73 330 52 467 23 335
Cuts at: 1494 1740 2752 2838 2850 3196 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 330 246 205
86 73 52 23 12 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1775 1938 1594
Size: 181 163 3300
Fragments arranged by size:
3300 181 163
BtsI GCAGTG_nn'
Cuts at: 887 887
Size: 3644
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 1229 1403 1794 1821 3166 1229
Size: 174 391 27 1345 1707
Fragments arranged by size:
1707 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 117 1667 1877 117
Size: 1550 210 1884
Fragments arranged by size:
1884 1550 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 336 616 1469 1901 2648 2916 140
Size: 196 280 853 432 747 268 868
Fragments arranged by size:
868 853 747 432 280 268 196
EcoO109I rG'GnC_Cy
Cuts at: 783 783
Size: 3644
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 1078 1326 2348 3327 1078
Size: 248 1022 979 1395
Fragments arranged by size:
1395 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1486 1516 1518 1548 1486
Size: 30 2 30 3582
Fragments arranged by size:
3582 30 30 2
HincII GTy'rAC
Cuts at: 683 683
Size: 3644
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 1405 1405
Size: 3644
MslI CAynn'nnrTG
Cuts at: 1633 1760 1633
Size: 127 3517
MspA1I CmG'CkG
Cuts at: 437 484 755 1069 1429 2442 2687 437
Size: 47 271 314 360 1013 245 1394
Fragments arranged by size:
1394 1013 360 314 271 245 47
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NdeI CA'TA_TG
Cuts at: 193 193
Size: 3644
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 3644
PfoI T'CCnGG_A
Cuts at: 156 156
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PstI C_TGCA'G
Cuts at: 442 691 1376 442
Size: 249 685 2710
Fragments arranged by size:
2710 685 249
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 437 484 1069 1429 437
Size: 47 585 360 2652
Fragments arranged by size:
2652 585 360 47
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 117 1667 1877 117
Size: 1550 210 1884
Fragments arranged by size:
1884 1550 210
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 438 687 1372 2365 2556 33
Size: 405 249 685 993 191 1121
Fragments arranged by size:
1121 993 685 405 249 191
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 334 465 666 334
Size: 131 201 3312
Fragments arranged by size:
3312 201 131
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StyI C'CwwG_G
Cuts at: 534 778 1132 534
Size: 244 354 3046
Fragments arranged by size:
3046 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1227 1360 1819 2028 1227
Size: 133 459 209 2843
Fragments arranged by size:
2843 459 209 133
TatI w'GTAC_w
Cuts at: 374 3159 374
Size: 2785 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1139 1139
Size: 3644
XmaI C'CCGG_G
Cuts at: 332 463 664 332
Size: 131 201 3312
Fragments arranged by size:
3312 201 131
XmnI GAAnn'nnTTC
Cuts at: 619 619
Size: 3644
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI