Restriction map for
pF1KE0523
(Circular) (Six-base) MAPSORT of: pF1KE0523.seq Check: 3736 from: 1 to: 3797
pF1KE0523 3797 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1622 2054 2801 3069 674
Size: 948 432 747 268 1402
Fragments arranged by size:
1402 948 747 432 268
AflIII A'CryG_T
Cuts at: 501 1149 2253 3230 501
Size: 648 1104 977 1068
Fragments arranged by size:
1104 1068 977 648
AgeI A'CCGG_T
Cuts at: 521 2185 521
Size: 1664 2133
AlwNI CAG_nnn'CTG
Cuts at: 1102 2669 1102
Size: 1567 2230
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 343 801 3150 3406 343
Size: 458 2349 256 734
Fragments arranged by size:
2349 734 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 813 1227 1475 1510 813
Size: 414 248 35 3100
Fragments arranged by size:
3100 414 248 35
BanII G_rGCy'C
Cuts at: 811 1841 811
Size: 1030 2767
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BbsI GAAGACnn'nnnn_
Cuts at: 371 371
Size: 3797
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 1422 1515 2571 1422
Size: 93 1056 2648
Fragments arranged by size:
2648 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 778
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 502 1150 502
Size: 648 3149
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 1228 1476 2178 3674 1228
Size: 248 702 1496 1351
Fragments arranged by size:
1496 1351 702 248
BsaWI w'CCGG_w
Cuts at: 521 986 1507 2185 2459 2606 3017 521
Size: 465 521 678 274 147 411 1301
Fragments arranged by size:
1301 678 521 465 411 274 147
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 811 1589 1779 2571 811
Size: 778 190 792 2037
Fragments arranged by size:
2037 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 811 1422 1515 1589 1779 1841 2571 811
Size: 611 93 74 190 62 730 2037
Fragments arranged by size:
2037 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 847 1061 1363 1744 290
Size: 557 214 302 381 2343
Fragments arranged by size:
2343 557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 521 1795 1976 2185 521
Size: 1274 181 209 2133
Fragments arranged by size:
2133 1274 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstYI r'GATC_y
Cuts at: 1 822 1289 1312 1647 1893 2905 2991 3003
Size: 821 467 23 335 246 1012 86 12
Cuts at: 3003 3349 1
Size: 346 449
Fragments arranged by size:
1012 821 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 323 323
Size: 3797
BtgI C'CryG_G
Cuts at: 107 266 107
Size: 159 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1040
Size: 3797
DraI TTT'AAA
Cuts at: 341 796 341
Size: 455 3342
DraIII CAC_nnn'GTG
Cuts at: 448 583 448
Size: 135 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 1382 1556 1947 1974 3319 1382
Size: 174 391 27 1345 1860
Fragments arranged by size:
1860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 770 1820 2030 770
Size: 1050 210 2537
Fragments arranged by size:
2537 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 778 1622 2054 2801 3069 674
Size: 104 844 432 747 268 1402
Fragments arranged by size:
1402 844 747 432 268 104
EcoO109I rG'GnC_Cy
Cuts at: 316 936 316
Size: 620 3177
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1816 1816
Size: 3797
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 1231 1479 2501 3480 1231
Size: 248 1022 979 1548
Fragments arranged by size:
1548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1669 1671 1701 1639
Size: 30 2 30 3735
Fragments arranged by size:
3735 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 692 1786 1913 692
Size: 1094 127 2576
Fragments arranged by size:
2576 1094 127
MspA1I CmG'CkG
Cuts at: 908 1222 1582 2595 2840 908
Size: 314 360 1013 245 1865
Fragments arranged by size:
1865 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 107 107
Size: 3797
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NspI r_CATG'y
Cuts at: 850 1350 2257 850
Size: 500 907 2390
Fragments arranged by size:
2390 907 500
PasI CC'CwG_GG
Cuts at: 155 155
Size: 3797
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PmlI CAC'GTG
Cuts at: 502 502
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PpuMI rG'GwC_Cy
Cuts at: 316 316
Size: 3797
PstI C_TGCA'G
Cuts at: 287 844 1529 287
Size: 557 685 2555
Fragments arranged by size:
2555 685 557
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 1222 1582 1222
Size: 360 3437
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 236 283 840 1525 2518 2709 33
Size: 203 47 557 685 993 191 1121
Fragments arranged by size:
1121 993 685 557 203 191 47
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 952 1867 2359 2621 952
Size: 915 492 262 2128
Fragments arranged by size:
2128 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StyI C'CwwG_G
Cuts at: 107 931 1285 107
Size: 824 354 2619
Fragments arranged by size:
2619 824 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 3314 3346 729
Size: 32 2553 32 1180
Fragments arranged by size:
2553 1180 32 32
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 779 779
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI