Restriction map for
pF1KE0518
(Circular) (Six-base) MAPSORT of: pF1KE0518.seq Check: 3224 from: 1 to: 3611
pF1KE0518 3611 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 3451 3451
Size: 3611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1436 1868 2615 2883 335
Size: 1101 432 747 268 1063
Fragments arranged by size:
1101 1063 747 432 268
AflIII A'CryG_T
Cuts at: 155 963 2067 3044 155
Size: 808 1104 977 722
Fragments arranged by size:
1104 977 808 722
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AleI CACnn'nnGTG
Cuts at: 160 160
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 615 2964 3220 615
Size: 2349 256 1006
Fragments arranged by size:
2349 1006 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 631 631
Size: 3611
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 303 627 1041 1289 1324 303
Size: 324 414 248 35 2590
Fragments arranged by size:
2590 414 324 248 35
BanII G_rGCy'C
Cuts at: 625 1655 625
Size: 1030 2581
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglI GCCn_nnn'nGGC
Cuts at: 385 385
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 235 723 235
Size: 488 3123
Bme1580I G_kGCm'C
Cuts at: 308 445 1236 1329 2385 308
Size: 137 791 93 1056 1534
Fragments arranged by size:
1534 1056 791 137 93
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 543 317
Size: 226 3385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3315 3315
Size: 3611
BsaAI yAC'GTr
Cuts at: 158 261 964 158
Size: 103 703 2805
Fragments arranged by size:
2805 703 103
BsaBI GATnn'nnATC
Cuts at: 6 1125 3162 6
Size: 1119 2037 455
Fragments arranged by size:
2037 1119 455
BsaHI Gr'CG_yC
Cuts at: 1042 1290 1992 3488 1042
Size: 248 702 1496 1165
Fragments arranged by size:
1496 1165 702 248
BsaWI w'CCGG_w
Cuts at: 800 1321 1999 2273 2420 2831 800
Size: 521 678 274 147 411 1580
Fragments arranged by size:
1580 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 591 591
Size: 3611
BseYI C'CCAG_C
Cuts at: 307 595 1036 1794 2371 2867 307
Size: 288 441 758 577 496 1051
Fragments arranged by size:
1051 758 577 496 441 288
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 625 1403 1593 2385 625
Size: 778 190 792 1851
Fragments arranged by size:
1851 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 308 445 625 1236 1329 1403 1593 1655 2385
Size: 137 180 611 93 74 190 62 730
Cuts at: 2385 308
Size: 1534
Fragments arranged by size:
1534 730 611 190 180 137 93 74
62
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 2787 2787
Size: 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 875 1177 1558 661
Size: 214 302 381 2714
Fragments arranged by size:
2714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 206 1903 1957 3441 3606 50
Size: 156 1697 54 1484 165 55
Fragments arranged by size:
1697 1484 165 156 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 1609 1790 1999 1609
Size: 181 209 3221
Fragments arranged by size:
3221 209 181
BssSI C'ACGA_G
Cuts at: 1882 2240 1882
Size: 358 3253
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstEII G'GTnAC_C
Cuts at: 336 336
Size: 3611
BstXI CCAn_nnnn'nTGG
Cuts at: 189 189
Size: 3611
BstYI r'GATC_y
Cuts at: 1 636 1103 1126 1461 1707 2719 2805 2817
Size: 635 467 23 335 246 1012 86 12
Cuts at: 2817 3163 1
Size: 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 124 341 124
Size: 217 3394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 1742 1905 1561
Size: 181 163 3267
Fragments arranged by size:
3267 181 163
BtsI GCAGTG_nn'
Cuts at: 443 854 443
Size: 411 3200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 474 509 474
Size: 35 3576
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 1317 2175 1317
Size: 858 2753
EaeI y'GGCC_r
Cuts at: 248 386 1196 1370 1761 1788 3133 248
Size: 138 810 174 391 27 1345 726
Fragments arranged by size:
1345 810 726 391 174 138 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 283 1819 2129 2275 3421 283
Size: 1536 310 146 1146 473
Fragments arranged by size:
1536 1146 473 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 335 543 1436 1868 2615 2883 317
Size: 18 208 893 432 747 268 1045
Fragments arranged by size:
1045 893 747 432 268 208 18
EcoNI CCTnn'n_nnAGG
Cuts at: 317 317
Size: 3611
EcoO109I rG'GnC_Cy
Cuts at: 750 750
Size: 3611
EcoRI G'AATT_C
Cuts at: 615 615
Size: 3611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1630 1630
Size: 3611
FspI TGC'GCA
Cuts at: 568 1392 568
Size: 824 2787
HaeII r_GCGC'y
Cuts at: 332 1045 1293 2315 3294 332
Size: 713 248 1022 979 649
Fragments arranged by size:
1022 979 713 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1453 1483 1485 1515 1453
Size: 30 2 30 3549
Fragments arranged by size:
3549 30 30 2
HincII GTy'rAC
Cuts at: 503 650 503
Size: 147 3464
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2466 2282
Size: 184 3427
MscI TGG'CCA
Cuts at: 250 388 1372 250
Size: 138 984 2489
Fragments arranged by size:
2489 984 138
MslI CAynn'nnrTG
Cuts at: 160 1600 1727 160
Size: 1440 127 2044
Fragments arranged by size:
2044 1440 127
MspA1I CmG'CkG
Cuts at: 126 220 455 722 1036 1396 2409 2654 126
Size: 94 235 267 314 360 1013 245 1083
Fragments arranged by size:
1083 1013 360 314 267 245 235 94
NaeI GCC'GGC
Cuts at: 1792 1792
Size: 3611
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NgoMIV G'CCGG_C
Cuts at: 1790 1790
Size: 3611
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PflMI CCAn_nnn'nTGG
Cuts at: 455 455
Size: 3611
PfoI T'CCnGG_A
Cuts at: 551 551
Size: 3611
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PmlI CAC'GTG
Cuts at: 158 158
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PstI C_TGCA'G
Cuts at: 658 1343 658
Size: 685 2926
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 1036 1396 1036
Size: 360 3251
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SacII CC_GC'GG
Cuts at: 127 127
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 658 658
Size: 3611
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SfcI C'TryA_G
Cuts at: 33 654 1339 2332 2523 33
Size: 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 191
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 633 633
Size: 3611
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StyI C'CwwG_G
Cuts at: 745 1099 745
Size: 354 3257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 150 1194 1327 1786 1995 150
Size: 1044 133 459 209 1766
Fragments arranged by size:
1766 1044 459 209 133
TatI w'GTAC_w
Cuts at: 3126 3126
Size: 3611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3579
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 1106 339
Size: 767 2844
XmaI C'CCGG_G
Cuts at: 631 631
Size: 3611
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI