Restriction map for
pF1KE0517
(Circular) (Six-base) MAPSORT of: pF1KE0517.seq Check: 3192 from: 1 to: 4040
pF1KE0517 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 540 553 582 540
Size: 13 29 3998
Fragments arranged by size:
3998 29 13
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 1006 1865 2297 3044 3312 473
Size: 533 859 432 747 268 1201
Fragments arranged by size:
1201 859 747 533 432 268
AfeI AGC'GCT
Cuts at: 484 484
Size: 4040
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1022 1022
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 243 264 574 909 984 1345 2912 243
Size: 21 310 335 75 361 1567 1371
Fragments arranged by size:
1567 1371 361 335 310 75 21
ApaI G_GGCC'C
Cuts at: 506 649 506
Size: 143 3897
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 207 1060 207
Size: 853 3187
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 655 1056 1470 1718 1753 655
Size: 401 414 248 35 2942
Fragments arranged by size:
2942 414 401 248 35
BanII G_rGCy'C
Cuts at: 506 649 1054 2084 506
Size: 143 405 1030 2462
Fragments arranged by size:
2462 1030 405 143
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbvCI CC'TCA_GC
Cuts at: 567 741 567
Size: 174 3866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1018 1152 1018
Size: 134 3906
Bme1580I G_kGCm'C
Cuts at: 506 649 660 948 963 1665 1758 2814 506
Size: 143 11 288 15 702 93 1056 1732
Fragments arranged by size:
1732 1056 702 288 143 93 15 11
BmrI ACTGGGnnnn_n'
Cuts at: 652 1252 1333 1668 3812 652
Size: 600 81 335 2144 880
Fragments arranged by size:
2144 880 600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 411 2407 411
Size: 1996 2044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 567 619 741 567
Size: 52 122 3866
Fragments arranged by size:
3866 122 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 283
Size: 30 4010
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 328
Size: 4040
BseYI C'CCAG_C
Cuts at: 250 594 598 686 869 1465 2223 2800 3296
Size: 344 4 88 183 596 758 577 496
Cuts at: 3296 250
Size: 994
Fragments arranged by size:
994 758 596 577 496 344 183 88
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1018 123
Size: 895 3145
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1832 2022 2814 1054
Size: 778 190 792 2280
Fragments arranged by size:
2280 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 151 151
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 506 649 660 948 963 1054 1665 1758 1832
Size: 143 11 288 15 91 611 93 74
Cuts at: 1832 2022 2084 2814 506
Size: 190 62 730 1732
Fragments arranged by size:
1732 730 611 288 190 143 93 91
74 62 15 11
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 540 553 582 1090 1304 1606 1987 540
Size: 13 29 508 214 302 381 2593
Fragments arranged by size:
2593 508 381 302 214 29 13
BsrBI CCG'CTC
Cuts at: 50 702 942 2332 2386 3870 4035 50
Size: 652 240 1390 54 1484 165 55
Fragments arranged by size:
1484 1390 652 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 130 461 585 2038 2219 2428 130
Size: 331 124 1453 181 209 1742
Fragments arranged by size:
1742 1453 331 209 181 124
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 909 1417 909
Size: 508 3532
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 168 724 168
Size: 556 3484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 117 1283 117
Size: 1166 2874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 366 401 366
Size: 35 4005
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 227 255 727 1031 1625 1799 2190 2217 3562
Size: 28 472 304 594 174 391 27 1345
Cuts at: 3562 227
Size: 705
Fragments arranged by size:
1345 705 594 472 391 304 174 28
27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 554 2063 2273 554
Size: 1509 210 2321
Fragments arranged by size:
2321 1509 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 2248 2558 2704 3850 114
Size: 2134 310 146 1146 304
Fragments arranged by size:
2134 1146 310 304 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 628 1006 1865 2297 3044 3312 473
Size: 155 378 859 432 747 268 1201
Fragments arranged by size:
1201 859 747 432 378 268 155
EcoNI CCTnn'n_nnAGG
Cuts at: 862 862
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 261 270 502 646 1179 261
Size: 9 232 144 533 3122
Fragments arranged by size:
3122 533 232 144 9
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
EcoRV GAT'ATC
Cuts at: 349 349
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 486 686 818 876 1474 1722 2744 3723 486
Size: 200 132 58 598 248 1022 979 803
Fragments arranged by size:
1022 979 803 598 248 200 132 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 767 799 1882 1912 1914 1944 262
Size: 32 473 32 1083 30 2 30 2358
Fragments arranged by size:
2358 1083 473 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 229 1801 229
Size: 1572 2468
MslI CAynn'nnrTG
Cuts at: 2029 2156 2029
Size: 127 3913
MspA1I CmG'CkG
Cuts at: 170 240 254 598 879 906 1017 1151 1465
Size: 70 14 344 281 27 111 134 314
Cuts at: 1465 1825 2838 3083 170
Size: 360 1013 245 1127
Fragments arranged by size:
1127 1013 360 344 314 281 245 134
111 70 27 14
NaeI GCC'GGC
Cuts at: 132 463 587 2221 132
Size: 331 124 1634 1951
Fragments arranged by size:
1951 1634 331 124
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 130 461 585 2219 130
Size: 331 124 1634 1951
Fragments arranged by size:
1951 1634 331 124
NspI r_CATG'y
Cuts at: 997 1093 1593 2500 997
Size: 96 500 907 2537
Fragments arranged by size:
2537 907 500 96
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 243 243
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 270 270
Size: 4040
PspOMI G'GGCC_C
Cuts at: 502 645 502
Size: 143 3897
PstI C_TGCA'G
Cuts at: 682 884 935 1087 1772 682
Size: 202 51 152 685 2950
Fragments arranged by size:
2950 685 202 152 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 598 879 1017 1465 1825 598
Size: 281 138 448 360 2813
Fragments arranged by size:
2813 448 360 281 138
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SacII CC_GC'GG
Cuts at: 171 171
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SanDI GG'GwC_CC
Cuts at: 270 270
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 4040
SfcI C'TryA_G
Cuts at: 33 678 880 931 1083 1768 2761 2952 33
Size: 645 202 51 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 202 191 152 51
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StuI AGG'CCT
Cuts at: 565 886 565
Size: 321 3719
StyI C'CwwG_G
Cuts at: 650 1174 1528 650
Size: 524 354 3162
Fragments arranged by size:
3162 524 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 1623 1756 2215 2424 643
Size: 980 133 459 209 2259
Fragments arranged by size:
2259 980 459 209 133
TatI w'GTAC_w
Cuts at: 284 3555 284
Size: 3271 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 534 566 3557 3589 534
Size: 32 2991 32 985
Fragments arranged by size:
2991 985 32 32
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BbsI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI