Restriction map for
pF1KE0512
(Circular) (Six-base) MAPSORT of: pF1KE0512.seq Check: 3161 from: 1 to: 3968
pF1KE0512 3968 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 143 1006 143
Size: 863 3105
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 2225 2972 3240 1793
Size: 432 747 268 2521
Fragments arranged by size:
2521 747 432 268
AflIII A'CryG_T
Cuts at: 200 698 1320 2424 3401 200
Size: 498 622 1104 977 767
Fragments arranged by size:
1104 977 767 622 498
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 807 825 1273 2840 807
Size: 18 448 1567 1935
Fragments arranged by size:
1935 1567 448 18
ApaI G_GGCC'C
Cuts at: 127 127
Size: 3968
ApaLI G'TGCA_C
Cuts at: 2738 2738
Size: 3968
ApoI r'AATT_y
Cuts at: 972 3321 3577 972
Size: 2349 256 1363
Fragments arranged by size:
2349 1363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 634 988 634
Size: 354 3614
BamHI G'GATC_C
Cuts at: 612 993 612
Size: 381 3587
BanI G'GyrC_C
Cuts at: 984 1398 1646 1681 984
Size: 414 248 35 3271
Fragments arranged by size:
3271 414 248 35
BanII G_rGCy'C
Cuts at: 127 470 512 635 662 929 982 2012 127
Size: 343 42 123 27 267 53 1030 2083
Fragments arranged by size:
2083 1030 343 267 123 53 42 27
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BbsI GAAGACnn'nnnn_
Cuts at: 219 219
Size: 3968
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 615 649 615
Size: 34 3934
BciVI GTATCCnnnnn_n'
Cuts at: 1866 2627 3968 1866
Size: 761 1341 1866
Fragments arranged by size:
1866 1341 761
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 127 883 1593 1686 2742 127
Size: 756 710 93 1056 1353
Fragments arranged by size:
1353 1056 756 710 93
BmgBI CAC'GTC
Cuts at: 203 897 203
Size: 694 3274
BmrI ACTGGGnnnn_n'
Cuts at: 285 507 1180 1261 1596 3740 285
Size: 222 673 81 335 2144 513
Fragments arranged by size:
2144 673 513 335 222 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 592
Size: 3968
Bpu10I CC'TnA_GC
Cuts at: 955 955
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 191 425 758 1157 1678 2356 2630 2777 3188
Size: 234 333 399 521 678 274 147 411
Cuts at: 3188 191
Size: 971
Fragments arranged by size:
971 678 521 411 399 333 274 234
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 3938
BseYI C'CCAG_C
Cuts at: 959 1393 2151 2728 3224 959
Size: 434 758 577 496 1703
Fragments arranged by size:
1703 758 577 496 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 831 457
Size: 374 3594
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 635 982 1760 1950 2742 635
Size: 347 778 190 792 1861
Fragments arranged by size:
1861 792 778 347 190
Bsp1286I G_dGCh'C
Cuts at: 127 470 512 635 662 883 929 982 1593
Size: 343 42 123 27 221 46 53 611
Cuts at: 1593 1686 1760 1950 2012 2742 127
Size: 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 343 221 190 123 93
74 62 53 46 42 27
BspEI T'CCGG_A
Cuts at: 191 425 3188 191
Size: 234 2763 971
Fragments arranged by size:
2763 971 234
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 350 896 1018 1232 1534 1915 350
Size: 546 122 214 302 381 2403
Fragments arranged by size:
2403 546 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 2260 2314 3798 3963 50
Size: 2210 54 1484 165 55
Fragments arranged by size:
2210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 1966 2147 2356 1966
Size: 181 209 3578
Fragments arranged by size:
3578 209 181
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstYI r'GATC_y
Cuts at: 1 612 993 1460 1483 1818 2064 3076 3162
Size: 611 381 467 23 335 246 1012 86
Cuts at: 3162 3174 3520 1
Size: 12 346 449
Fragments arranged by size:
1012 611 467 449 381 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 740 740
Size: 3968
BtgI C'CryG_G
Cuts at: 335 566 335
Size: 231 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 730 1211 730
Size: 481 3487
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DraIII CAC_nnn'GTG
Cuts at: 878 878
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 170 827 1553 1727 2118 2145 3490 170
Size: 657 726 174 391 27 1345 648
Fragments arranged by size:
1345 726 657 648 391 174 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 633 980 633
Size: 347 3621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1793 2225 2972 3240 592
Size: 1201 432 747 268 1320
Fragments arranged by size:
1320 1201 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 484 484
Size: 3968
EcoO109I rG'GnC_Cy
Cuts at: 208 489 669 822 1107 208
Size: 281 180 153 285 3069
Fragments arranged by size:
3069 285 281 180 153
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 204 1987 204
Size: 1783 2185
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 1402 1650 2672 3651 1402
Size: 248 1022 979 1719
Fragments arranged by size:
1719 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1810 1840 1842 1872 1810
Size: 30 2 30 3906
Fragments arranged by size:
3906 30 30 2
HincII GTy'rAC
Cuts at: 1007 1007
Size: 3968
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 525 2639 2823 347
Size: 178 2114 184 1492
Fragments arranged by size:
2114 1492 184 178
MscI TGG'CCA
Cuts at: 172 829 1729 172
Size: 657 900 2411
Fragments arranged by size:
2411 900 657
MslI CAynn'nnrTG
Cuts at: 112 1957 2084 112
Size: 1845 127 1996
Fragments arranged by size:
1996 1845 127
MspA1I CmG'CkG
Cuts at: 415 807 1079 1393 1753 2766 3011 415
Size: 392 272 314 360 1013 245 1372
Fragments arranged by size:
1372 1013 392 360 314 272 245
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 3968
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 3968
NspI r_CATG'y
Cuts at: 289 702 1021 1521 2428 289
Size: 413 319 500 907 1829
Fragments arranged by size:
1829 907 500 413 319
PasI CC'CwG_GG
Cuts at: 839 839
Size: 3968
PciI A'CATG_T
Cuts at: 698 2424 698
Size: 1726 2242
PflMI CCAn_nnn'nTGG
Cuts at: 264 264
Size: 3968
PfoI T'CCnGG_A
Cuts at: 215 590 608 215
Size: 375 18 3575
Fragments arranged by size:
3575 375 18
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PpuMI rG'GwC_Cy
Cuts at: 208 489 669 822 208
Size: 281 180 153 3354
Fragments arranged by size:
3354 281 180 153
PspOMI G'GGCC_C
Cuts at: 123 123
Size: 3968
PstI C_TGCA'G
Cuts at: 1015 1700 1015
Size: 685 3283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 807 1393 1753 807
Size: 586 360 3022
Fragments arranged by size:
3022 586 360
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 635 982 635
Size: 347 3621
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 940 3485 940
Size: 2545 1423
SexAI A'CCwGG_T
Cuts at: 704 704
Size: 3968
SfcI C'TryA_G
Cuts at: 33 1011 1696 2689 2880 33
Size: 978 685 993 191 1121
Fragments arranged by size:
1121 993 978 685 191
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 289 1021 1521 289
Size: 732 500 2736
Fragments arranged by size:
2736 732 500
StuI AGG'CCT
Cuts at: 562 562
Size: 3968
StyI C'CwwG_G
Cuts at: 1102 1456 1102
Size: 354 3614
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 452 831 1551 1684 2143 2352 452
Size: 379 720 133 459 209 2068
Fragments arranged by size:
2068 720 459 379 209 133
TatI w'GTAC_w
Cuts at: 695 938 3483 695
Size: 243 2545 1180
Fragments arranged by size:
2545 1180 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 192 224 499 531 3485 3517 192
Size: 32 275 32 2954 32 643
Fragments arranged by size:
2954 643 275 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 223 271 667 1765 223
Size: 48 396 1098 2426
Fragments arranged by size:
2426 1098 396 48
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 446 1463 446
Size: 1017 2951
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI