Restriction map for
pF1KE0511
(Circular) (Six-base) MAPSORT of: pF1KE0511.seq Check: 6502 from: 1 to: 4040
pF1KE0511 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1018 1078 1018
Size: 60 3980
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 767 1865 2297 3044 3312 659
Size: 108 1098 432 747 268 1387
Fragments arranged by size:
1387 1098 747 432 268 108
AflIII A'CryG_T
Cuts at: 873 1392 2496 3473 873
Size: 519 1104 977 1440
Fragments arranged by size:
1440 1104 977 519
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 161 831 161
Size: 670 3370
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 241 404 553 855 1044 3393 3649 241
Size: 163 149 302 189 2349 256 632
Fragments arranged by size:
2349 632 302 256 189 163 149
AseI AT'TA_AT
Cuts at: 20 704 20
Size: 684 3356
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 420 1065 420
Size: 645 3395
BanI G'GyrC_C
Cuts at: 197 1056 1470 1718 1753 197
Size: 859 414 248 35 2484
Fragments arranged by size:
2484 859 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 452 452
Size: 4040
BbvCI CC'TCA_GC
Cuts at: 257 257
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 505 1938 2699 4040 505
Size: 1433 761 1341 505
Fragments arranged by size:
1433 1341 761 505
BclI T'GATC_A
Cuts at: 729 1560 729
Size: 831 3209
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 843
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 257 257
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1216 2095 2587 2885 475
Size: 741 879 492 298 1630
Fragments arranged by size:
1630 879 741 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 209 579 1554 3591 6
Size: 203 370 975 2037 455
Fragments arranged by size:
2037 975 455 370 203
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 505
Size: 30 4010
BseYI C'CCAG_C
Cuts at: 1465 2223 2800 3296 1465
Size: 758 577 496 2209
Fragments arranged by size:
2209 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1832 2022 2814 1054
Size: 778 190 792 2280
Fragments arranged by size:
2280 792 778 190
BsmI GAATG_Cn'
Cuts at: 820 820
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 1054 1665 1758 1832 2022 2084 2814 1054
Size: 611 93 74 190 62 730 2280
Fragments arranged by size:
2280 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 411 3216 411
Size: 2805 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 924 924
Size: 4040
BstYI r'GATC_y
Cuts at: 1 420 939 1065 1532 1555 1890 2136 3148
Size: 419 519 126 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 519 467 449 419 346 335 246
126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 155 155
Size: 4040
BtgI C'CryG_G
Cuts at: 107 107
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 524 1283 524
Size: 759 3281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 954 989 954
Size: 35 4005
DraI TTT'AAA
Cuts at: 399 1039 399
Size: 640 3400
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 788 1625 1799 2190 2217 3562 788
Size: 837 174 391 27 1345 1266
Fragments arranged by size:
1345 1266 837 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 627 791 2063 2273 627
Size: 164 1272 210 2394
Fragments arranged by size:
2394 1272 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 767 843 1865 2297 3044 3312 659
Size: 108 76 1022 432 747 268 1387
Fragments arranged by size:
1387 1022 747 432 268 108 76
EcoO109I rG'GnC_Cy
Cuts at: 459 1179 459
Size: 720 3320
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 164 205 469 2059 164
Size: 41 264 1590 2145
Fragments arranged by size:
2145 1590 264 41
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 1882 1912 1914 1944 259
Size: 32 1591 30 2 30 2355
Fragments arranged by size:
2355 1591 32 30 30 2
HincII GTy'rAC
Cuts at: 764 1079 764
Size: 315 3725
HindIII A'AGCT_T
Cuts at: 170 170
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 790 1801 790
Size: 1011 3029
MslI CAynn'nnrTG
Cuts at: 192 347 2029 2156 192
Size: 155 1682 127 2076
Fragments arranged by size:
2076 1682 155 127
MspA1I CmG'CkG
Cuts at: 821 1151 1465 1825 2838 3083 821
Size: 330 314 360 1013 245 1778
Fragments arranged by size:
1778 1013 360 330 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NdeI CA'TA_TG
Cuts at: 564 564
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 877 1093 1593 2500 877
Size: 216 500 907 2417
Fragments arranged by size:
2417 907 500 216
PciI A'CATG_T
Cuts at: 873 2496 873
Size: 1623 2417
PfoI T'CCnGG_A
Cuts at: 474 474
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 459 459
Size: 4040
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 821 1465 1825 821
Size: 644 360 3036
Fragments arranged by size:
3036 644 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 1083 1768 2761 2952 33
Size: 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 454 1195 2110 2602 2864 454
Size: 741 915 492 262 1630
Fragments arranged by size:
1630 915 741 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
SspI AAT'ATT
Cuts at: 702 702
Size: 4040
StuI AGG'CCT
Cuts at: 608 608
Size: 4040
StyI C'CwwG_G
Cuts at: 107 1174 1528 107
Size: 1067 354 2619
Fragments arranged by size:
2619 1067 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 407 407
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI