Restriction map for
pF1KE0508
(Circular) (Six-base) MAPSORT of: pF1KE0508.seq Check: 1246 from: 1 to: 3617
pF1KE0508 3617 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 527 1442 1874 2621 2889 423
Size: 104 915 432 747 268 1151
Fragments arranged by size:
1151 915 747 432 268 104
AflIII A'CryG_T
Cuts at: 143 969 2073 3050 143
Size: 826 1104 977 710
Fragments arranged by size:
1104 977 826 710
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 376
Size: 32 3585
AlwNI CAG_nnn'CTG
Cuts at: 316 462 922 2489 316
Size: 146 460 1567 1444
Fragments arranged by size:
1567 1444 460 146
ApaLI G'TGCA_C
Cuts at: 235 2387 235
Size: 2152 1465
ApoI r'AATT_y
Cuts at: 621 2970 3226 621
Size: 2349 256 1012
Fragments arranged by size:
2349 1012 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 285 637 285
Size: 352 3265
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 633 1047 1295 1330 633
Size: 414 248 35 2920
Fragments arranged by size:
2920 414 248 35
BanII G_rGCy'C
Cuts at: 586 631 1661 586
Size: 45 1030 2542
Fragments arranged by size:
2542 1030 45
BbeI G_GCGC'C
Cuts at: 1051 1299 1051
Size: 248 3369
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2276 3617 1515
Size: 761 1341 1515
Fragments arranged by size:
1515 1341 761
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 178 729 178
Size: 551 3066
Bme1580I G_kGCm'C
Cuts at: 239 1242 1335 2391 239
Size: 1003 93 1056 1465
Fragments arranged by size:
1465 1056 1003 93
BmrI ACTGGGnnnn_n'
Cuts at: 476 829 910 1245 3389 476
Size: 353 81 335 2144 704
Fragments arranged by size:
2144 704 353 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 387 1984 387
Size: 1597 2020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 293 382 557 258
Size: 35 89 175 3318
Fragments arranged by size:
3318 175 89 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1672 2164 2462 793
Size: 879 492 298 1948
Fragments arranged by size:
1948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 144 970 144
Size: 826 2791
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 1048 1296 1998 3494 1048
Size: 248 702 1496 1171
Fragments arranged by size:
1496 1171 702 248
BsaWI w'CCGG_w
Cuts at: 129 806 1327 2005 2279 2426 2837 129
Size: 677 521 678 274 147 411 909
Fragments arranged by size:
909 678 677 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 376 388 406 358
Size: 18 12 18 3569
Fragments arranged by size:
3569 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 363
Size: 3617
BseYI C'CCAG_C
Cuts at: 192 1042 1800 2377 2873 192
Size: 850 758 577 496 936
Fragments arranged by size:
936 850 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 239 586 631 1409 1599 2391 239
Size: 347 45 778 190 792 1465
Fragments arranged by size:
1465 792 778 347 190 45
Bsp1286I G_dGCh'C
Cuts at: 239 586 631 1242 1335 1409 1599 1661 2391
Size: 347 45 611 93 74 190 62 730
Cuts at: 2391 239
Size: 1465
Fragments arranged by size:
1465 730 611 347 190 93 74 62
45
BspEI T'CCGG_A
Cuts at: 2837 2837
Size: 3617
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 667 881 1183 1564 506
Size: 161 214 302 381 2559
Fragments arranged by size:
2559 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 295 1529 295
Size: 1234 2383
BsrFI r'CCGG_y
Cuts at: 1615 1796 2005 1615
Size: 181 209 3227
Fragments arranged by size:
3227 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstXI CCAn_nnnn'nTGG
Cuts at: 165 192 165
Size: 27 3590
BstYI r'GATC_y
Cuts at: 1 563 642 1109 1132 1467 1713 2725 2811
Size: 562 79 467 23 335 246 1012 86
Cuts at: 2811 2823 3169 1
Size: 12 346 449
Fragments arranged by size:
1012 562 467 449 346 335 246 86
79 23 12
BtgI C'CryG_G
Cuts at: 107 587 107
Size: 480 3137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 267 860 267
Size: 593 3024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 507 542 507
Size: 35 3582
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 603 1202 1376 1767 1794 3139 603
Size: 599 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 599 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 486 1825 2135 2281 3427 486
Size: 1339 310 146 1146 676
Fragments arranged by size:
1339 1146 676 310 146
EcoICRI GAG'CTC
Cuts at: 584 629 584
Size: 45 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 293 382 423 527 557 1442 1874 2621
Size: 35 89 41 104 30 885 432 747
Cuts at: 2621 2889 258
Size: 268 986
Fragments arranged by size:
986 885 747 432 268 104 89 41
35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 575 575
Size: 3617
EcoO109I rG'GnC_Cy
Cuts at: 756 756
Size: 3617
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3617
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 1051 1299 2321 3300 1051
Size: 248 1022 979 1368
Fragments arranged by size:
1368 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 555 587 1459 1489 1491 1521 487
Size: 32 36 32 872 30 2 30 2583
Fragments arranged by size:
2583 872 36 32 32 30 30 2
HincII GTy'rAC
Cuts at: 656 656
Size: 3617
KasI G'GCGC_C
Cuts at: 1047 1295 1047
Size: 248 3369
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MfeI C'AATT_G
Cuts at: 426 426
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2472 2288
Size: 184 3433
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 232 242 1606 1733 232
Size: 10 1364 127 2116
Fragments arranged by size:
2116 1364 127 10
MspA1I CmG'CkG
Cuts at: 278 728 1042 1402 2415 2660 278
Size: 450 314 360 1013 245 1235
Fragments arranged by size:
1235 1013 450 360 314 245
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 1048 1296 1048
Size: 248 3369
NcoI C'CATG_G
Cuts at: 107 107
Size: 3617
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PasI CC'CwG_GG
Cuts at: 378 378
Size: 3617
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PfoI T'CCnGG_A
Cuts at: 548 573 548
Size: 25 3592
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 408 2756 2788 376
Size: 32 2348 32 1205
Fragments arranged by size:
2348 1205 32 32
PstI C_TGCA'G
Cuts at: 664 1349 664
Size: 685 2932
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 278 1042 1402 278
Size: 764 360 2493
Fragments arranged by size:
2493 764 360
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 586 631 586
Size: 45 3572
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 3134 3134
Size: 3617
SfcI C'TryA_G
Cuts at: 33 660 1345 2338 2529 33
Size: 627 685 993 191 1121
Fragments arranged by size:
1121 993 685 627 191
SfoI GGC'GCC
Cuts at: 1049 1297 1049
Size: 248 3369
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 287 639 287
Size: 352 3265
SmlI C'TyrA_G
Cuts at: 772 1687 2179 2441 772
Size: 915 492 262 1948
Fragments arranged by size:
1948 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StuI AGG'CCT
Cuts at: 164 406 164
Size: 242 3375
StyI C'CwwG_G
Cuts at: 107 751 1105 107
Size: 644 354 2619
Fragments arranged by size:
2619 644 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1200 1333 1792 2001 1200
Size: 133 459 209 2816
Fragments arranged by size:
2816 459 209 133
TatI w'GTAC_w
Cuts at: 334 3132 334
Size: 2798 819
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3585
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1112 1112
Size: 3617
XmaI C'CCGG_G
Cuts at: 285 637 285
Size: 352 3265
XmnI GAAnn'nnTTC
Cuts at: 356 356
Size: 3617
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI