Restriction map for
pF1KE0504
(Circular) (Six-base) MAPSORT of: pF1KE0504.seq Check: 9892 from: 1 to: 3752
pF1KE0504 3752 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2009 2756 3024 1577
Size: 432 747 268 2305
Fragments arranged by size:
2305 747 432 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 365 2140 365
Size: 1775 1977
AlwNI CAG_nnn'CTG
Cuts at: 264 1057 2624 264
Size: 793 1567 1392
Fragments arranged by size:
1567 1392 793
ApaI G_GGCC'C
Cuts at: 462 660 687 462
Size: 198 27 3527
Fragments arranged by size:
3527 198 27
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 453 469 772 453
Size: 16 303 3433
Fragments arranged by size:
3433 303 16
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 768 1182 1430 1465 768
Size: 414 248 35 3055
Fragments arranged by size:
3055 414 248 35
BanII G_rGCy'C
Cuts at: 462 660 687 766 1796 462
Size: 198 27 79 1030 2418
Fragments arranged by size:
2418 1030 198 79 27
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 462 660 687 1377 1470 2526 462
Size: 198 27 690 93 1056 1688
Fragments arranged by size:
1688 1056 690 198 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 518 364
Size: 154 3598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 560 3456 560
Size: 2896 856
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 520 1183 1431 2133 3629 520
Size: 663 248 702 1496 643
Fragments arranged by size:
1496 702 663 643 248
BsaWI w'CCGG_w
Cuts at: 365 941 1462 2140 2414 2561 2972 365
Size: 576 521 678 274 147 411 1145
Fragments arranged by size:
1145 678 576 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 505
Size: 30 3722
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 262 286 307 361 526 529 582 193
Size: 69 24 21 54 165 3 53 3363
Fragments arranged by size:
3363 165 69 54 53 24 21 3
BseYI C'CCAG_C
Cuts at: 704 1177 1935 2512 3008 704
Size: 473 758 577 496 1448
Fragments arranged by size:
1448 758 577 496 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 415
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 147 154 1294 1340 2548 104
Size: 43 7 1140 46 1208 1308
Fragments arranged by size:
1308 1208 1140 46 43 7
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 721 721
Size: 3752
Bsp1286I G_dGCh'C
Cuts at: 462 660 687 766 1377 1470 1544 1734 1796
Size: 198 27 79 611 93 74 190 62
Cuts at: 1796 2526 462
Size: 730 1688
Fragments arranged by size:
1688 730 611 198 190 93 79 74
62 27
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 146 365 1750 1931 2140 146
Size: 219 1385 181 209 1758
Fragments arranged by size:
1758 1385 219 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 587 777 1244 1267 1602 1848 2860 2946
Size: 586 190 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 586 467 449 346 335 246 190
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 485 485
Size: 3752
BtgI C'CryG_G
Cuts at: 107 527 107
Size: 420 3332
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 151 1337 1511 1902 1929 3274 151
Size: 1186 174 391 27 1345 629
Fragments arranged by size:
1345 1186 629 391 174 27
EagI C'GGCC_G
Cuts at: 151 1337 151
Size: 1186 2566
EarI CTCTTCn'nnn_
Cuts at: 178 329 1775 1985 178
Size: 151 1446 210 1945
Fragments arranged by size:
1945 1446 210 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 518 1577 2009 2756 3024 364
Size: 154 1059 432 747 268 1092
Fragments arranged by size:
1092 1059 747 432 268 154
EcoNI CCTnn'n_nnAGG
Cuts at: 488 552 488
Size: 64 3688
EcoO109I rG'GnC_Cy
Cuts at: 458 683 684 891 458
Size: 225 1 207 3319
Fragments arranged by size:
3319 225 207 1
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 300 711 1186 1434 2456 3435 300
Size: 411 475 248 1022 979 617
Fragments arranged by size:
1022 979 617 475 411 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 508 537 540 630 662 1594 1624 1626
Size: 3 29 3 90 32 932 30 2
Cuts at: 1626 1656 505
Size: 30 2601
Fragments arranged by size:
2601 932 90 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
HindIII A'AGCT_T
Cuts at: 283 283
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 1741 1868 1741
Size: 127 3625
MspA1I CmG'CkG
Cuts at: 261 585 740 863 1177 1537 2550 2795 261
Size: 324 155 123 314 360 1013 245 1218
Fragments arranged by size:
1218 1013 360 324 314 245 155 123
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 616 805 1305 2212 616
Size: 189 500 907 2156
Fragments arranged by size:
2156 907 500 189
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PspOMI G'GGCC_C
Cuts at: 458 656 683 458
Size: 198 27 3527
Fragments arranged by size:
3527 198 27
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 907 1822 2314 2576 907
Size: 915 492 262 2083
Fragments arranged by size:
2083 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 107 679 886 1240 107
Size: 572 207 354 2619
Fragments arranged by size:
2619 572 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 387 1549 387
Size: 1162 2590
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 1247 441
Size: 806 2946
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI