Restriction map for
pF1KE0502
(Circular) (Six-base) MAPSORT of: pF1KE0502.seq Check: 4605 from: 1 to: 3746
pF1KE0502 3746 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 620 1098 2202 3179 620
Size: 478 1104 977 1187
Fragments arranged by size:
1187 1104 977 478
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 611 762 1176 1424 1459 611
Size: 151 414 248 35 2898
Fragments arranged by size:
2898 414 248 151 35
BanII G_rGCy'C
Cuts at: 230 280 531 760 1790 230
Size: 50 251 229 1030 2186
Fragments arranged by size:
2186 1030 251 229 50
BbeI G_GCGC'C
Cuts at: 615 1180 1428 615
Size: 565 248 2933
Fragments arranged by size:
2933 565 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 121 155 121
Size: 34 3712
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 244 518 1371 1464 2520 244
Size: 274 853 93 1056 1470
Fragments arranged by size:
1470 1056 853 274 93
BmgBI CAC'GTC
Cuts at: 513 578 513
Size: 65 3681
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 370 286
Size: 84 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 155 3450 155
Size: 3295 451
BsaAI yAC'GTr
Cuts at: 621 1099 621
Size: 478 3268
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 612 662 1177 1425 2127 3623 612
Size: 50 515 248 702 1496 735
Fragments arranged by size:
1496 735 702 515 248 50
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 244 371 471 517 733 1171 1929 2506 3002
Size: 127 100 46 216 438 758 577 496
Cuts at: 3002 244
Size: 988
Fragments arranged by size:
988 758 577 496 438 216 127 100
46
BsiEI CG_ry'CG
Cuts at: 104 236 1288 1334 2542 104
Size: 132 1052 46 1208 1308
Fragments arranged by size:
1308 1208 1052 132 46
BsiHKAI G_wGCw'C
Cuts at: 129 230 760 1538 1728 2520 129
Size: 101 530 778 190 792 1355
Fragments arranged by size:
1355 792 778 530 190 101
Bsp1286I G_dGCh'C
Cuts at: 129 230 244 280 518 531 760 1371 1464
Size: 101 14 36 238 13 229 611 93
Cuts at: 1464 1538 1728 1790 2520 129
Size: 74 190 62 730 1355
Fragments arranged by size:
1355 730 611 238 229 190 101 93
74 62 36 14 13
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 630 2038 2092 3576 3741 50
Size: 580 1408 54 1484 165 55
Fragments arranged by size:
1484 1408 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 221 687 1123 221
Size: 466 436 2844
Fragments arranged by size:
2844 466 436
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstXI CCAn_nnnn'nTGG
Cuts at: 288 288
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 156 301 330 606 107
Size: 49 145 29 276 3247
Fragments arranged by size:
3247 276 145 49 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 233 483 1331 1505 1896 1923 3268 233
Size: 250 848 174 391 27 1345 711
Fragments arranged by size:
1345 848 711 391 250 174 27
EagI C'GGCC_G
Cuts at: 233 1331 233
Size: 1098 2648
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1954 2264 2410 3556 127
Size: 1827 310 146 1146 317
Fragments arranged by size:
1827 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 228 758 228
Size: 530 3216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 370 1571 2003 2750 3018 286
Size: 84 1201 432 747 268 1014
Fragments arranged by size:
1201 1014 747 432 268 84
EcoO109I rG'GnC_Cy
Cuts at: 323 885 323
Size: 562 3184
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 204 1765 204
Size: 1561 2185
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 615 1180 1428 2450 3429 615
Size: 565 248 1022 979 932
Fragments arranged by size:
1022 979 932 565 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 1588 1618 1620 1650 367
Size: 32 1189 30 2 30 2463
Fragments arranged by size:
2463 1189 32 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 611 1176 1424 611
Size: 565 248 2933
Fragments arranged by size:
2933 565 248
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 2417 2601 410
Size: 2007 184 1555
Fragments arranged by size:
2007 1555 184
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 286 650 1735 1862 286
Size: 364 1085 127 2170
Fragments arranged by size:
2170 1085 364 127
MspA1I CmG'CkG
Cuts at: 145 332 678 857 1171 1531 2544 2789 145
Size: 187 346 179 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 360 346 314 245 187 179
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 612 1177 1425 612
Size: 565 248 2933
Fragments arranged by size:
2933 565 248
NcoI C'CATG_G
Cuts at: 107 156 107
Size: 49 3697
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PflMI CCAn_nnn'nTGG
Cuts at: 524 524
Size: 3746
PfoI T'CCnGG_A
Cuts at: 539 539
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PstI C_TGCA'G
Cuts at: 144 683 793 1478 144
Size: 539 110 685 2412
Fragments arranged by size:
2412 685 539 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 145 678 1171 1531 145
Size: 533 493 360 2360
Fragments arranged by size:
2360 533 493 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 230 760 230
Size: 530 3216
SacII CC_GC'GG
Cuts at: 333 333
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 140 679 789 1474 2467 2658 33
Size: 107 539 110 685 993 191 1121
Fragments arranged by size:
1121 993 685 539 191 110 107
SfoI GGC'GCC
Cuts at: 613 1178 1426 613
Size: 565 248 2933
Fragments arranged by size:
2933 565 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
SspI AAT'ATT
Cuts at: 432 432
Size: 3746
StyI C'CwwG_G
Cuts at: 107 156 165 210 880 1234 107
Size: 49 9 45 670 354 2619
Fragments arranged by size:
2619 670 354 49 45 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 1329 1462 1921 2130 268
Size: 1061 133 459 209 1884
Fragments arranged by size:
1884 1061 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 163 1241 163
Size: 1078 2668
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI