Restriction map for
pF1KE0496
(Circular) (Six-base) MAPSORT of: pF1KE0496.seq Check: 8277 from: 1 to: 3869
pF1KE0496 3869 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 198 198
Size: 3869
AccI GT'mk_AC
Cuts at: 635 907 635
Size: 272 3597
Acc65I G'GTAC_C
Cuts at: 716 885 716
Size: 169 3700
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 1694 2126 2873 3141 627
Size: 1067 432 747 268 1355
Fragments arranged by size:
1355 1067 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AhdI GACnn_n'nnGTC
Cuts at: 409 409
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 163 629 792 1174 2741 163
Size: 466 163 382 1567 1291
Fragments arranged by size:
1567 1291 466 382 163
ApaI G_GGCC'C
Cuts at: 186 186
Size: 3869
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 331 873 3222 3478 331
Size: 542 2349 256 722
Fragments arranged by size:
2349 722 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 336 823 889 336
Size: 487 66 3316
Fragments arranged by size:
3316 487 66
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 716 818 885 1299 1547 1582 716
Size: 102 67 414 248 35 3003
Fragments arranged by size:
3003 414 248 102 67 35
BanII G_rGCy'C
Cuts at: 186 857 883 1913 186
Size: 671 26 1030 2142
Fragments arranged by size:
2142 1030 671 26
BbeI G_GCGC'C
Cuts at: 1303 1551 1303
Size: 248 3621
BbsI GAAGACnn'nnnn_
Cuts at: 288 288
Size: 3869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 110 144 317 351 110
Size: 34 173 34 3628
Fragments arranged by size:
3628 173 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 710 1767 2528 3869 710
Size: 1057 761 1341 710
Fragments arranged by size:
1341 1057 761 710
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglI GCCn_nnn'nGGC
Cuts at: 145 259 145
Size: 114 3755
BglII A'GATC_T
Cuts at: 1384 3421 1384
Size: 2037 1832
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 186 1494 1587 2643 186
Size: 1308 93 1056 1412
Fragments arranged by size:
1412 1308 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 617 1081 1162 1497 3641 617
Size: 464 81 335 2144 845
Fragments arranged by size:
2144 845 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 657 475
Size: 182 3687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 675 3573 675
Size: 2898 971
BsaAI yAC'GTr
Cuts at: 1222 1222
Size: 3869
BsaBI GATnn'nnATC
Cuts at: 6 448 1383 3420 6
Size: 442 935 2037 455
Fragments arranged by size:
2037 935 455 442
BsaHI Gr'CG_yC
Cuts at: 1300 1548 2250 3746 1300
Size: 248 702 1496 1423
Fragments arranged by size:
1496 1423 702 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 544
Size: 30 3839
BseYI C'CCAG_C
Cuts at: 1294 2052 2629 3125 1294
Size: 758 577 496 2038
Fragments arranged by size:
2038 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 375 1411 1457 2665 104
Size: 271 1036 46 1208 1308
Fragments arranged by size:
1308 1208 1036 271 46
BsiHKAI G_wGCw'C
Cuts at: 349 554 883 1661 1851 2643 349
Size: 205 329 778 190 792 1575
Fragments arranged by size:
1575 792 778 329 205 190
Bsp1286I G_dGCh'C
Cuts at: 186 349 554 857 883 1494 1587 1661 1851
Size: 163 205 303 26 611 93 74 190
Cuts at: 1851 1913 2643 186
Size: 62 730 1412
Fragments arranged by size:
1412 730 611 303 205 190 163 93
74 62 26
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 919 1133 1435 1816 198
Size: 721 214 302 381 2251
Fragments arranged by size:
2251 721 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 1867 2048 2257 1867
Size: 181 209 3479
Fragments arranged by size:
3479 209 181
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 792 1246 792
Size: 454 3415
BstBI TT'CG_AA
Cuts at: 299 2230 299
Size: 1931 1938
BstXI CCAn_nnnn'nTGG
Cuts at: 537 537
Size: 3869
BstYI r'GATC_y
Cuts at: 1 602 894 1361 1384 1719 1965 2977 3063
Size: 601 292 467 23 335 246 1012 86
Cuts at: 3063 3075 3421 1
Size: 12 346 449
Fragments arranged by size:
1012 601 467 449 346 335 292 246
86 23 12
BtgI C'CryG_G
Cuts at: 363 398 435 711 363
Size: 35 37 276 3521
Fragments arranged by size:
3521 276 37 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3525
Fragments arranged by size:
3525 181 163
BtsI GCAGTG_nn'
Cuts at: 510 820 1112 510
Size: 310 292 3267
Fragments arranged by size:
3267 310 292
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 137 845 1454 1628 2019 2046 3391 137
Size: 708 609 174 391 27 1345 615
Fragments arranged by size:
1345 708 615 609 391 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 2077 2387 2533 3679 277
Size: 1800 310 146 1146 467
Fragments arranged by size:
1800 1146 467 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 627 657 1694 2126 2873 3141 475
Size: 152 30 1037 432 747 268 1203
Fragments arranged by size:
1203 1037 747 432 268 152 30
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 3869
EcoO109I rG'GnC_Cy
Cuts at: 160 182 183 403 830 1008 160
Size: 22 1 220 427 178 3021
Fragments arranged by size:
3021 427 220 178 22 1
EcoRI G'AATT_C
Cuts at: 331 873 331
Size: 542 3327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 382 1650 382
Size: 1268 2601
HaeII r_GCGC'y
Cuts at: 254 1303 1551 2573 3552 254
Size: 1049 248 1022 979 571
Fragments arranged by size:
1049 1022 979 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 395 427 1711 1741 1743 1773 395
Size: 32 1284 30 2 30 2491
Fragments arranged by size:
2491 1284 32 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 1299 1547 1299
Size: 248 3621
KpnI G_GTAC'C
Cuts at: 720 889 720
Size: 169 3700
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 2540 2724 452
Size: 2088 184 1597
Fragments arranged by size:
2088 1597 184
MscI TGG'CCA
Cuts at: 847 1630 847
Size: 783 3086
MslI CAynn'nnrTG
Cuts at: 535 755 1858 1985 535
Size: 220 1103 127 2419
Fragments arranged by size:
2419 1103 220 127
MspA1I CmG'CkG
Cuts at: 400 437 713 980 1294 1654 2667 2912 400
Size: 37 276 267 314 360 1013 245 1357
Fragments arranged by size:
1357 1013 360 314 276 267 245 37
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 1300 1548 1300
Size: 248 3621
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PasI CC'CwG_GG
Cuts at: 157 407 157
Size: 250 3619
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PflMI CCAn_nnn'nTGG
Cuts at: 629 629
Size: 3869
PfoI T'CCnGG_A
Cuts at: 452 452
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PpuMI rG'GwC_Cy
Cuts at: 403 830 403
Size: 427 3442
PspOMI G'GGCC_C
Cuts at: 182 182
Size: 3869
PspXI vC'TCGA_Gb
Cuts at: 823 823
Size: 3869
PstI C_TGCA'G
Cuts at: 195 916 1601 195
Size: 721 685 2463
Fragments arranged by size:
2463 721 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SacII CC_GC'GG
Cuts at: 401 438 714 401
Size: 37 276 3556
Fragments arranged by size:
3556 276 37
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SanDI GG'GwC_CC
Cuts at: 403 403
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 195 916 195
Size: 721 3148
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 191 282 912 1597 2590 2781 33
Size: 158 91 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191 158 91
SfoI GGC'GCC
Cuts at: 1301 1549 1301
Size: 248 3621
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 891 891
Size: 3869
SmlI C'TyrA_G
Cuts at: 823 1024 1939 2431 2693 823
Size: 201 915 492 262 1999
Fragments arranged by size:
1999 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StuI AGG'CCT
Cuts at: 127 127
Size: 3869
StyI C'CwwG_G
Cuts at: 170 254 860 1003 1357 170
Size: 84 606 143 354 2682
Fragments arranged by size:
2682 606 354 143 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 636 1452 1585 2044 2253 636
Size: 816 133 459 209 2252
Fragments arranged by size:
2252 816 459 209 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 544 576 3386 3418 544
Size: 32 2810 32 995
Fragments arranged by size:
2810 995 32 32
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XhoI C'TCGA_G
Cuts at: 823 823
Size: 3869
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3869
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI