Restriction map for
pF1KE0495
(Circular) (Six-base) MAPSORT of: pF1KE0495.seq Check: 8741 from: 1 to: 3734
pF1KE0495 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1991 2738 3006 1559
Size: 432 747 268 2287
Fragments arranged by size:
2287 747 432 268
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 1039 2606 1039
Size: 1567 2167
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 738 3087 3343 738
Size: 2349 256 1129
Fragments arranged by size:
2349 1129 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 453 754 453
Size: 301 3433
BamHI G'GATC_C
Cuts at: 719 759 719
Size: 40 3694
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 682 748 1778 682
Size: 66 1030 2638
Fragments arranged by size:
2638 1030 66
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 505 846 505
Size: 341 3393
Bme1580I G_kGCm'C
Cuts at: 241 1359 1452 2508 241
Size: 1118 93 1056 1467
Fragments arranged by size:
1467 1118 1056 93
BmgBI CAC'GTC
Cuts at: 570 570
Size: 3734
BmrI ACTGGGnnnn_n'
Cuts at: 591 946 1027 1362 3506 591
Size: 355 81 335 2144 819
Fragments arranged by size:
2144 819 355 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 696 2101 696
Size: 1405 2329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 511 271
Size: 240 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 515 1248 3285 6
Size: 509 733 2037 455
Fragments arranged by size:
2037 733 509 455
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 649 923 1444 2122 2396 2543 2954 649
Size: 274 521 678 274 147 411 1429
Fragments arranged by size:
1429 678 521 411 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 287
Size: 30 3704
BseYI C'CCAG_C
Cuts at: 241 368 1159 1917 2494 2990 241
Size: 127 791 758 577 496 985
Fragments arranged by size:
985 791 758 577 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 233 1276 1322 2530 104
Size: 129 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 129 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2508 748
Size: 778 190 792 1974
Fragments arranged by size:
1974 792 778 190
BsmI GAATG_Cn'
Cuts at: 640 640
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 241 682 748 1359 1452 1526 1716 1778 2508
Size: 441 66 611 93 74 190 62 730
Cuts at: 2508 241
Size: 1467
Fragments arranged by size:
1467 730 611 441 190 93 74 66
62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 717 784 998 1300 1681 717
Size: 67 214 302 381 2770
Fragments arranged by size:
2770 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 157 555 1646 157
Size: 398 1091 2245
Fragments arranged by size:
2245 1091 398
BsrFI r'CCGG_y
Cuts at: 212 1732 1913 2122 212
Size: 1520 181 209 1824
Fragments arranged by size:
1824 1520 209 181
BssSI C'ACGA_G
Cuts at: 220 2005 2363 220
Size: 1785 358 1591
Fragments arranged by size:
1785 1591 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 249 249
Size: 3734
BstYI r'GATC_y
Cuts at: 1 719 759 1226 1249 1584 1830 2842 2928
Size: 718 40 467 23 335 246 1012 86
Cuts at: 2928 2940 3286 1
Size: 12 346 449
Fragments arranged by size:
1012 718 467 449 346 335 246 86
40 23 12
BtgI C'CryG_G
Cuts at: 533 533
Size: 3734
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 524 1684 1865 2028 524
Size: 1160 181 163 2230
Fragments arranged by size:
2230 1160 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 230 1319 1493 1884 1911 3256 230
Size: 1089 174 391 27 1345 708
Fragments arranged by size:
1345 1089 708 391 174 27
EagI C'GGCC_G
Cuts at: 230 1319 230
Size: 1089 2645
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 511 1559 1991 2738 3006 271
Size: 240 1048 432 747 268 999
Fragments arranged by size:
1048 999 747 432 268 240
EcoO109I rG'GnC_Cy
Cuts at: 169 560 581 873 169
Size: 391 21 292 3030
Fragments arranged by size:
3030 391 292 21
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 650 1723 1850 650
Size: 1073 127 2534
Fragments arranged by size:
2534 1073 127
MspA1I CmG'CkG
Cuts at: 166 845 1159 1519 2532 2777 166
Size: 679 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 679 360 314 245
NaeI GCC'GGC
Cuts at: 214 1915 214
Size: 1701 2033
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NcoI C'CATG_G
Cuts at: 533 533
Size: 3734
NgoMIV G'CCGG_C
Cuts at: 212 1913 212
Size: 1701 2033
NspI r_CATG'y
Cuts at: 319 787 1287 2194 319
Size: 468 500 907 1859
Fragments arranged by size:
1859 907 500 468
PasI CC'CwG_GG
Cuts at: 115 115
Size: 3734
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 534 539 534
Size: 5 3729
PfoI T'CCnGG_A
Cuts at: 668 668
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PpuMI rG'GwC_Cy
Cuts at: 169 560 169
Size: 391 3343
PspXI vC'TCGA_Gb
Cuts at: 453 453
Size: 3734
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3049
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SanDI GG'GwC_CC
Cuts at: 169 169
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 777 1462 2455 2646 33
Size: 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 453 889 1804 2296 2558 453
Size: 436 915 492 262 1629
Fragments arranged by size:
1629 915 492 436 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 319 787 1287 319
Size: 468 500 2766
Fragments arranged by size:
2766 500 468
StyI C'CwwG_G
Cuts at: 327 533 868 1222 327
Size: 206 335 354 2839
Fragments arranged by size:
2839 354 335 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 1317 1450 1909 2118 291
Size: 1026 133 459 209 1907
Fragments arranged by size:
1907 1026 459 209 133
TatI w'GTAC_w
Cuts at: 338 3249 338
Size: 2911 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 3251 3283 287
Size: 32 2932 32 738
Fragments arranged by size:
2932 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 574 1229 574
Size: 655 3079
XhoI C'TCGA_G
Cuts at: 453 453
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI