Restriction map for
pF1KE0487
(Circular) (Six-base) MAPSORT of: pF1KE0487.seq Check: 1227 from: 1 to: 4184
pF1KE0487 4184 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 329 737 329
Size: 408 3776
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 2009 2441 3188 3456 919
Size: 1090 432 747 268 1647
Fragments arranged by size:
1647 1090 747 432 268
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1028 1028
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 195 1048 1159 1489 3056 195
Size: 853 111 330 1567 1323
Fragments arranged by size:
1567 1323 853 330 111
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 1188 3537 3793 1188
Size: 2349 256 1579
Fragments arranged by size:
2349 1579 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1204 1204
Size: 4184
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 529 1200 1614 1862 1897 529
Size: 671 414 248 35 2816
Fragments arranged by size:
2816 671 414 248 35
BanII G_rGCy'C
Cuts at: 1198 2228 1198
Size: 1030 3154
BbeI G_GCGC'C
Cuts at: 533 1618 1866 533
Size: 1085 248 2851
Fragments arranged by size:
2851 1085 248
BbsI GAAGACnn'nnnn_
Cuts at: 917 917
Size: 4184
BbvCI CC'TCA_GC
Cuts at: 511 511
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 975 2082 2843 4184 975
Size: 1107 761 1341 975
Fragments arranged by size:
1341 1107 975 761
BclI T'GATC_A
Cuts at: 825 1704 825
Size: 879 3305
BfrBI ATG'CAT
Cuts at: 1084 1084
Size: 4184
BglII A'GATC_T
Cuts at: 1022 1699 3736 1022
Size: 677 2037 1470
Fragments arranged by size:
2037 1470 677
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 653 1809 1902 2958 653
Size: 1156 93 1056 1879
Fragments arranged by size:
1879 1156 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 490 586 751 1190 325
Size: 165 96 165 439 3319
Fragments arranged by size:
3319 439 165 165 96
Bpu10I CC'TnA_GC
Cuts at: 511 511
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1360 2239 2731 3029 725
Size: 635 879 492 298 1880
Fragments arranged by size:
1880 879 635 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 808 3888 808
Size: 3080 1104
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1049 1698 3735 6
Size: 1043 649 2037 455
Fragments arranged by size:
2037 1043 649 455
BsaHI Gr'CG_yC
Cuts at: 218 326 530 665 734 1615 1863 2565 4061
Size: 108 204 135 69 881 248 702 1496
Cuts at: 4061 218
Size: 341
Fragments arranged by size:
1496 881 702 341 248 204 135 108
69
BsaWI w'CCGG_w
Cuts at: 176 338 392 482 1373 1894 2572 2846 2993
Size: 162 54 90 891 521 678 274 147
Cuts at: 2993 3404 176
Size: 411 956
Fragments arranged by size:
956 891 678 521 411 274 162 147
90 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1163 1193 1163
Size: 30 4154
BseYI C'CCAG_C
Cuts at: 427 1609 2367 2944 3440 427
Size: 1182 758 577 496 1171
Fragments arranged by size:
1182 1171 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 931 298
Size: 633 3551
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 884 1198 1976 2166 2958 884
Size: 314 778 190 792 2110
Fragments arranged by size:
2110 792 778 314 190
BsmBI CGTCTCn'nnnn_
Cuts at: 317 317
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 653 884 1198 1809 1902 1976 2166 2228 2958
Size: 231 314 611 93 74 190 62 730
Cuts at: 2958 653
Size: 1879
Fragments arranged by size:
1879 730 611 314 231 190 93 74
62
BspEI T'CCGG_A
Cuts at: 176 338 392 482 3404 176
Size: 162 54 90 2922 956
Fragments arranged by size:
2922 956 162 90 54
BspHI T'CATG_A
Cuts at: 822 3360 822
Size: 2538 1646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 569 1234 1448 1750 2131 569
Size: 665 214 302 381 2622
Fragments arranged by size:
2622 665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 578 2182 2363 2572 578
Size: 1604 181 209 2190
Fragments arranged by size:
2190 1604 209 181
BssSI C'ACGA_G
Cuts at: 258 2455 2813 258
Size: 2197 358 1629
Fragments arranged by size:
2197 1629 358
BstAPI GCAn_nnn'nTGC
Cuts at: 888 1561 888
Size: 673 3511
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 1074 1074
Size: 4184
BstYI r'GATC_y
Cuts at: 1 1022 1209 1676 1699 2034 2280 3292 3378
Size: 1021 187 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1021 1012 467 449 346 335 246 187
86 23 12
BtgI C'CryG_G
Cuts at: 107 533 596 107
Size: 426 63 3695
Fragments arranged by size:
3695 426 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 431 2134 2315 2478 431
Size: 1703 181 163 2137
Fragments arranged by size:
2137 1703 181 163
BtsI GCAGTG_nn'
Cuts at: 236 1427 236
Size: 1191 2993
DraI TTT'AAA
Cuts at: 164 1183 164
Size: 1019 3165
DrdI GACnn_nn'nnGTC
Cuts at: 751 1890 2748 751
Size: 1139 858 2187
Fragments arranged by size:
2187 1139 858
EaeI y'GGCC_r
Cuts at: 576 1769 1943 2334 2361 3706 576
Size: 1193 174 391 27 1345 1054
Fragments arranged by size:
1345 1193 1054 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 1109 2207 2417 1109
Size: 1098 210 2876
Fragments arranged by size:
2876 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2392 2702 2848 3994 2392
Size: 310 146 1146 2582
Fragments arranged by size:
2582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 490 586 751 919 1190 2009 2441 3188
Size: 165 96 165 168 271 819 432 747
Cuts at: 3188 3456 325
Size: 268 1053
Fragments arranged by size:
1053 819 747 432 271 268 168 165
165 96
EcoNI CCTnn'n_nnAGG
Cuts at: 410 451 565 410
Size: 41 114 4029
Fragments arranged by size:
4029 114 41
EcoO109I rG'GnC_Cy
Cuts at: 363 630 840 1323 363
Size: 267 210 483 3224
Fragments arranged by size:
3224 483 267 210
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 447 2203 447
Size: 1756 2428
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 533 1618 1866 2888 3867 533
Size: 1085 248 1022 979 850
Fragments arranged by size:
1085 1022 979 850 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 390 422 2026 2056 2058 2088 390
Size: 32 1604 30 2 30 2486
Fragments arranged by size:
2486 1604 32 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
KasI G'GCGC_C
Cuts at: 529 1614 1862 529
Size: 1085 248 2851
Fragments arranged by size:
2851 1085 248
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3039 2855
Size: 184 4000
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 892 2173 2300 892
Size: 1281 127 2776
Fragments arranged by size:
2776 1281 127
MspA1I CmG'CkG
Cuts at: 303 1295 1609 1969 2982 3227 303
Size: 992 314 360 1013 245 1260
Fragments arranged by size:
1260 1013 992 360 314 245
NaeI GCC'GGC
Cuts at: 580 2365 580
Size: 1785 2399
NarI GG'CG_CC
Cuts at: 530 1615 1863 530
Size: 1085 248 2851
Fragments arranged by size:
2851 1085 248
NcoI C'CATG_G
Cuts at: 107 533 596 107
Size: 426 63 3695
Fragments arranged by size:
3695 426 63
NgoMIV G'CCGG_C
Cuts at: 578 2363 578
Size: 1785 2399
NsiI A_TGCA'T
Cuts at: 1086 1086
Size: 4184
NspI r_CATG'y
Cuts at: 891 1088 1237 1737 2644 891
Size: 197 149 500 907 2431
Fragments arranged by size:
2431 907 500 197 149
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PfoI T'CCnGG_A
Cuts at: 437 993 437
Size: 556 3628
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 363 630 363
Size: 267 3917
PshAI GACnn'nnGTC
Cuts at: 217 628 217
Size: 411 3773
PstI C_TGCA'G
Cuts at: 297 503 1231 1916 297
Size: 206 728 685 2565
Fragments arranged by size:
2565 728 685 206
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 303 1609 1969 303
Size: 1306 360 2518
Fragments arranged by size:
2518 1306 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 293 499 1227 1912 2905 3096 33
Size: 260 206 728 685 993 191 1121
Fragments arranged by size:
1121 993 728 685 260 206 191
SfoI GGC'GCC
Cuts at: 531 1616 1864 531
Size: 1085 248 2851
Fragments arranged by size:
2851 1085 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 740 1339 2254 2746 3008 740
Size: 599 915 492 262 1916
Fragments arranged by size:
1916 915 599 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1088 1237 1737 1088
Size: 149 500 3535
Fragments arranged by size:
3535 500 149
StuI AGG'CCT
Cuts at: 571 571
Size: 4184
StyI C'CwwG_G
Cuts at: 107 533 596 626 1318 1672 107
Size: 426 63 30 692 354 2619
Fragments arranged by size:
2619 692 426 354 63 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 603 1679 114
Size: 489 1076 2619
Fragments arranged by size:
2619 1076 489
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
ZraI GAC'GTC
Cuts at: 327 735 327
Size: 408 3776
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BseRI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI