Restriction map for
pF1KE0485
(Circular) (Six-base) MAPSORT of: pF1KE0485.seq Check: 2687 from: 1 to: 3710
pF1KE0485 3710 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 748 748
Size: 3710
Acc65I G'GTAC_C
Cuts at: 726 726
Size: 3710
AclI AA'CG_TT
Cuts at: 704 3550 704
Size: 2846 864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1535 1967 2714 2982 433
Size: 1102 432 747 268 1161
Fragments arranged by size:
1161 1102 747 432 268
AflIII A'CryG_T
Cuts at: 462 1062 2166 3143 462
Size: 600 1104 977 1029
Fragments arranged by size:
1104 1029 977 600
AgeI A'CCGG_T
Cuts at: 2098 2098
Size: 3710
AleI CACnn'nnGTG
Cuts at: 463 601 463
Size: 138 3572
AlwNI CAG_nnn'CTG
Cuts at: 1015 2582 1015
Size: 1567 2143
ApaI G_GGCC'C
Cuts at: 134 552 134
Size: 418 3292
ApaLI G'TGCA_C
Cuts at: 2480 2480
Size: 3710
ApoI r'AATT_y
Cuts at: 577 714 3063 3319 577
Size: 137 2349 256 968
Fragments arranged by size:
2349 968 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
AvaI C'yCGr_G
Cuts at: 730 730
Size: 3710
BamHI G'GATC_C
Cuts at: 147 735 147
Size: 588 3122
BanI G'GyrC_C
Cuts at: 235 726 1140 1388 1423 235
Size: 491 414 248 35 2522
Fragments arranged by size:
2522 491 414 248 35
BanII G_rGCy'C
Cuts at: 134 406 552 724 1754 134
Size: 272 146 172 1030 2090
Fragments arranged by size:
2090 1030 272 172 146
BbeI G_GCGC'C
Cuts at: 1144 1392 1144
Size: 248 3462
BciVI GTATCCnnnnn_n'
Cuts at: 1608 2369 3710 1608
Size: 761 1341 1608
Fragments arranged by size:
1608 1341 761
BclI T'GATC_A
Cuts at: 1230 1230
Size: 3710
BglII A'GATC_T
Cuts at: 371 1225 3262 371
Size: 854 2037 819
Fragments arranged by size:
2037 854 819
BlpI GC'TnA_GC
Cuts at: 822 822
Size: 3710
Bme1580I G_kGCm'C
Cuts at: 134 552 1335 1428 2484 134
Size: 418 783 93 1056 1360
Fragments arranged by size:
1360 1056 783 418 93
BmrI ACTGGGnnnn_n'
Cuts at: 922 1003 1338 3482 922
Size: 81 335 2144 1150
Fragments arranged by size:
2144 1150 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 3710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 673 420
Size: 253 3457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1765 2257 2555 886
Size: 879 492 298 2041
Fragments arranged by size:
2041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3414 3414
Size: 3710
BsaAI yAC'GTr
Cuts at: 465 1063 465
Size: 598 3112
BsaBI GATnn'nnATC
Cuts at: 6 1224 3261 6
Size: 1218 2037 455
Fragments arranged by size:
2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 1141 1389 2091 3587 1141
Size: 248 702 1496 1264
Fragments arranged by size:
1496 1264 702 248
BsaWI w'CCGG_w
Cuts at: 899 1420 2098 2372 2519 2930 899
Size: 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 120 544 590 1135 1893 2470 2966 120
Size: 424 46 545 758 577 496 864
Fragments arranged by size:
864 758 577 545 496 424 46
BsiEI CG_ry'CG
Cuts at: 104 312 1252 1298 2506 104
Size: 208 940 46 1208 1308
Fragments arranged by size:
1308 1208 940 208 46
BsiHKAI G_wGCw'C
Cuts at: 653 724 1502 1692 2484 653
Size: 71 778 190 792 1879
Fragments arranged by size:
1879 792 778 190 71
Bsp1286I G_dGCh'C
Cuts at: 134 406 552 653 724 1335 1428 1502 1692
Size: 272 146 101 71 611 93 74 190
Cuts at: 1692 1754 2484 134
Size: 62 730 1360
Fragments arranged by size:
1360 730 611 272 190 146 101 93
74 71 62
BspEI T'CCGG_A
Cuts at: 2930 2930
Size: 3710
BspHI T'CATG_A
Cuts at: 563 2886 563
Size: 2323 1387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 974 1276 1657 760
Size: 214 302 381 2813
Fragments arranged by size:
2813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2002 2056 3540 3705 50
Size: 1952 54 1484 165 55
Fragments arranged by size:
1952 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1622 1622
Size: 3710
BsrFI r'CCGG_y
Cuts at: 1708 1889 2098 1708
Size: 181 209 3320
Fragments arranged by size:
3320 209 181
BssSI C'ACGA_G
Cuts at: 1981 2339 1981
Size: 358 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 534 1087 534
Size: 553 3157
BstBI TT'CG_AA
Cuts at: 2071 2071
Size: 3710
BstYI r'GATC_y
Cuts at: 1 147 371 735 1202 1225 1560 1806 2818
Size: 146 224 364 467 23 335 246 1012
Cuts at: 2818 2904 2916 3262 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 364 346 335 246 224
146 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1660 1841 2004 1660
Size: 181 163 3366
Fragments arranged by size:
3366 181 163
BtsI GCAGTG_nn'
Cuts at: 523 585 953 523
Size: 62 368 3280
Fragments arranged by size:
3280 368 62
DraI TTT'AAA
Cuts at: 709 709
Size: 3710
DrdI GACnn_nn'nnGTC
Cuts at: 1416 2274 1416
Size: 858 2852
EaeI y'GGCC_r
Cuts at: 309 1295 1469 1860 1887 3232 309
Size: 986 174 391 27 1345 787
Fragments arranged by size:
1345 986 787 391 174 27
EagI C'GGCC_G
Cuts at: 309 1295 309
Size: 986 2724
EarI CTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1918 2228 2374 3520 1918
Size: 310 146 1146 2108
Fragments arranged by size:
2108 1146 310 146
EcoICRI GAG'CTC
Cuts at: 722 722
Size: 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 433 673 1535 1967 2714 2982 420
Size: 13 240 862 432 747 268 1148
Fragments arranged by size:
1148 862 747 432 268 240 13
EcoNI CCTnn'n_nnAGG
Cuts at: 514 514
Size: 3710
EcoO109I rG'GnC_Cy
Cuts at: 548 849 548
Size: 301 3409
EcoRI G'AATT_C
Cuts at: 714 714
Size: 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1729 1729
Size: 3710
FspI TGC'GCA
Cuts at: 1491 1491
Size: 3710
HaeII r_GCGC'y
Cuts at: 225 1144 1392 2414 3393 225
Size: 919 248 1022 979 542
Fragments arranged by size:
1022 979 919 542 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 1552 1582 1584 1614 461
Size: 32 1059 30 2 30 2557
Fragments arranged by size:
2557 1059 32 30 30 2
HincII GTy'rAC
Cuts at: 749 749
Size: 3710
KasI G'GCGC_C
Cuts at: 1140 1388 1140
Size: 248 3462
KpnI G_GTAC'C
Cuts at: 730 730
Size: 3710
MluI A'CGCG_T
Cuts at: 3143 3143
Size: 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 338 2381 2565 191
Size: 147 2043 184 1336
Fragments arranged by size:
2043 1336 184 147
MscI TGG'CCA
Cuts at: 1471 1471
Size: 3710
MslI CAynn'nnrTG
Cuts at: 463 601 1699 1826 463
Size: 138 1098 127 2347
Fragments arranged by size:
2347 1098 138 127
MspA1I CmG'CkG
Cuts at: 821 1135 1495 2508 2753 821
Size: 314 360 1013 245 1778
Fragments arranged by size:
1778 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1891 1891
Size: 3710
NarI GG'CG_CC
Cuts at: 1141 1389 1141
Size: 248 3462
NcoI C'CATG_G
Cuts at: 107 107
Size: 3710
NgoMIV G'CCGG_C
Cuts at: 1889 1889
Size: 3710
NspI r_CATG'y
Cuts at: 763 1263 2170 763
Size: 500 907 2303
Fragments arranged by size:
2303 907 500
PasI CC'CwG_GG
Cuts at: 632 632
Size: 3710
PciI A'CATG_T
Cuts at: 2166 2166
Size: 3710
PfoI T'CCnGG_A
Cuts at: 397 397
Size: 3710
PmeI GTTT'AAAC
Cuts at: 709 709
Size: 3710
PmlI CAC'GTG
Cuts at: 465 465
Size: 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 3678
PspOMI G'GGCC_C
Cuts at: 130 548 130
Size: 418 3292
PstI C_TGCA'G
Cuts at: 757 1442 757
Size: 685 3025
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3710
PvuII CAG'CTG
Cuts at: 1135 1495 1135
Size: 360 3350
RsrII CG'GwC_CG
Cuts at: 1905 1905
Size: 3710
SacI G_AGCT'C
Cuts at: 724 724
Size: 3710
SalI G'TCGA_C
Cuts at: 747 747
Size: 3710
SapI GCTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
SbfI CC_TGCA'GG
Cuts at: 757 757
Size: 3710
ScaI AGT'ACT
Cuts at: 3227 3227
Size: 3710
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 3710
SfcI C'TryA_G
Cuts at: 33 375 753 1438 2431 2622 33
Size: 342 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 378 342 191
SfoI GGC'GCC
Cuts at: 1142 1390 1142
Size: 248 3462
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
SmaI CCC'GGG
Cuts at: 732 732
Size: 3710
SmlI C'TyrA_G
Cuts at: 865 1780 2272 2534 865
Size: 915 492 262 2041
Fragments arranged by size:
2041 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3710
SphI G_CATG'C
Cuts at: 763 1263 763
Size: 500 3210
StyI C'CwwG_G
Cuts at: 107 844 1198 107
Size: 737 354 2619
Fragments arranged by size:
2619 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 1293 1426 1885 2094 231
Size: 1062 133 459 209 1847
Fragments arranged by size:
1847 1062 459 209 133
TatI w'GTAC_w
Cuts at: 3225 3225
Size: 3710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 3678
Tth111I GACn'n_nGTC
Cuts at: 1507 1507
Size: 3710
XbaI T'CTAG_A
Cuts at: 741 741
Size: 3710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 634 664 1205 141
Size: 493 30 541 2646
Fragments arranged by size:
2646 541 493 30
XmaI C'CCGG_G
Cuts at: 730 730
Size: 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI