Restriction map for
pF1KE0473
(Circular) (Six-base) MAPSORT of: pF1KE0473.seq Check: 6783 from: 1 to: 3704
pF1KE0473 3704 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 635 1529 1961 2708 2976 437
Size: 198 894 432 747 268 1165
Fragments arranged by size:
1165 894 747 432 268 198
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 599 662 599
Size: 63 3641
AlwNI CAG_nnn'CTG
Cuts at: 175 187 199 1009 2576 175
Size: 12 12 810 1567 1303
Fragments arranged by size:
1567 1303 810 12 12
ApaI G_GGCC'C
Cuts at: 360 360
Size: 3704
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 724 724
Size: 3704
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 496 720 1134 1382 1417 496
Size: 224 414 248 35 2783
Fragments arranged by size:
2783 414 248 224 35
BanII G_rGCy'C
Cuts at: 360 718 1748 360
Size: 358 1030 2316
Fragments arranged by size:
2316 1030 358
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 566 816 566
Size: 250 3454
Bme1580I G_kGCm'C
Cuts at: 360 499 1329 1422 2478 360
Size: 139 830 93 1056 1586
Fragments arranged by size:
1586 1056 830 139 93
BmgBI CAC'GTC
Cuts at: 579 579
Size: 3704
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 146 152 332 140
Size: 6 6 180 3512
Fragments arranged by size:
3512 180 6 6
Bpu10I CC'TnA_GC
Cuts at: 125 125
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3408 3408
Size: 3704
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 322 1218 3255 6
Size: 316 896 2037 455
Fragments arranged by size:
2037 896 455 316
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 299 893 1414 2092 2366 2513 2924 299
Size: 594 521 678 274 147 411 1079
Fragments arranged by size:
1079 678 594 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 613 643 613
Size: 30 3674
BseYI C'CCAG_C
Cuts at: 192 399 634 1129 1887 2464 2960 192
Size: 207 235 495 758 577 496 936
Fragments arranged by size:
936 758 577 496 495 235 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 3704
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 718 1496 1686 2478 718
Size: 778 190 792 1944
Fragments arranged by size:
1944 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 442 442
Size: 3704
Bsp1286I G_dGCh'C
Cuts at: 360 499 718 1329 1422 1496 1686 1748 2478
Size: 139 219 611 93 74 190 62 730
Cuts at: 2478 360
Size: 1586
Fragments arranged by size:
1586 730 611 219 190 139 93 74
62
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 225 754 968 1270 1651 225
Size: 529 214 302 381 2278
Fragments arranged by size:
2278 529 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 552 1616 552
Size: 1064 2640
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910
Size: 728 467 23 335 246 1012 86 12
Cuts at: 2910 3256 1
Size: 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 200 200
Size: 3704
BtgI C'CryG_G
Cuts at: 107 107
Size: 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 251 947 251
Size: 696 3008
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 333 1289 1463 1854 1881 3226 333
Size: 956 174 391 27 1345 811
Fragments arranged by size:
1345 956 811 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 263 413 1727 1937 263
Size: 150 1314 210 2030
Fragments arranged by size:
2030 1314 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 146 152 332 437 635 1529 1961 2708
Size: 6 6 180 105 198 894 432 747
Cuts at: 2708 2976 140
Size: 268 868
Fragments arranged by size:
894 868 747 432 268 198 180 105
6 6
EcoO109I rG'GnC_Cy
Cuts at: 357 519 843 357
Size: 162 324 3218
Fragments arranged by size:
3218 324 162
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 1723 264
Size: 1459 2245
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 1138 1386 2408 3387 1138
Size: 248 1022 979 1455
Fragments arranged by size:
1455 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 556 1693 1820 556
Size: 1137 127 2440
Fragments arranged by size:
2440 1137 127
MspA1I CmG'CkG
Cuts at: 627 815 1129 1489 2502 2747 627
Size: 188 314 360 1013 245 1584
Fragments arranged by size:
1584 1013 360 314 245 188
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 107
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 757 1257 2164 757
Size: 500 907 2297
Fragments arranged by size:
2297 907 500
PasI CC'CwG_GG
Cuts at: 360 591 360
Size: 231 3473
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PspOMI G'GGCC_C
Cuts at: 356 356
Size: 3704
PstI C_TGCA'G
Cuts at: 470 751 1436 470
Size: 281 685 2738
Fragments arranged by size:
2738 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 627 1129 1489 627
Size: 502 360 2842
Fragments arranged by size:
2842 502 360
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 466 747 1432 2425 2616 33
Size: 433 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 433 281 191
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StuI AGG'CCT
Cuts at: 283 283
Size: 3704
StyI C'CwwG_G
Cuts at: 107 209 838 1192 107
Size: 102 629 354 2619
Fragments arranged by size:
2619 629 354 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 550 1287 1420 1879 2088 550
Size: 737 133 459 209 2166
Fragments arranged by size:
2166 737 459 209 133
TatI w'GTAC_w
Cuts at: 348 3219 348
Size: 2871 833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BseRI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI