Restriction map for
pF1KE0470
(Circular) (Six-base) MAPSORT of: pF1KE0470.seq Check: 2220 from: 1 to: 4016
pF1KE0470 4016 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 188 1032 188
Size: 844 3172
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1841 2273 3020 3288 1841
Size: 432 747 268 2569
Fragments arranged by size:
2569 747 432 268
AflIII A'CryG_T
Cuts at: 872 1368 2472 3449 872
Size: 496 1104 977 1439
Fragments arranged by size:
1439 1104 977 496
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AleI CACnn'nnGTG
Cuts at: 637 757 637
Size: 120 3896
AlwNI CAG_nnn'CTG
Cuts at: 910 1321 2888 910
Size: 411 1567 2038
Fragments arranged by size:
2038 1567 411
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 1006 1020 3369 3625 1006
Size: 14 2349 256 1397
Fragments arranged by size:
2349 1397 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 188 397 681 913 1032 1446 1694 1729 188
Size: 209 284 232 119 414 248 35 2475
Fragments arranged by size:
2475 414 284 248 232 209 119 35
BanII G_rGCy'C
Cuts at: 1030 2060 1030
Size: 1030 2986
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 990 1024 990
Size: 34 3982
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BfrBI ATG'CAT
Cuts at: 608 608
Size: 4016
BglII A'GATC_T
Cuts at: 767 1531 3568 767
Size: 764 2037 1215
Fragments arranged by size:
2037 1215 764
BlpI GC'TnA_GC
Cuts at: 454 1128 454
Size: 674 3342
Bme1580I G_kGCm'C
Cuts at: 686 1641 1734 2790 686
Size: 955 93 1056 1912
Fragments arranged by size:
1912 1056 955 93
BmrI ACTGGGnnnn_n'
Cuts at: 1228 1309 1644 3788 1228
Size: 81 335 2144 1456
Fragments arranged by size:
2144 1456 335 81
BmtI G_CTAG'C
Cuts at: 260 260
Size: 4016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 862
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 433 1192 2071 2563 2861 285
Size: 148 759 879 492 298 1440
Fragments arranged by size:
1440 879 759 492 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 1369 1369
Size: 4016
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 323 323
Size: 4016
BseYI C'CCAG_C
Cuts at: 657 1441 2199 2776 3272 657
Size: 784 758 577 496 1401
Fragments arranged by size:
1401 784 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 578 923 984 556
Size: 22 345 61 3588
Fragments arranged by size:
3588 345 61 22
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1030 1808 1998 2790 1030
Size: 778 190 792 2256
Fragments arranged by size:
2256 792 778 190
BsmI GAATG_Cn'
Cuts at: 292 813 292
Size: 521 3495
Bsp1286I G_dGCh'C
Cuts at: 686 1030 1641 1734 1808 1998 2060 2790 686
Size: 344 611 93 74 190 62 730 1912
Fragments arranged by size:
1912 730 611 344 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 1066 1280 1582 1963 176
Size: 890 214 302 381 2229
Fragments arranged by size:
2229 890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 923 1928 923
Size: 1005 3011
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4016
BstYI r'GATC_y
Cuts at: 1 767 1041 1508 1531 1866 2112 3124 3210
Size: 766 274 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 766 467 449 346 335 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 401 752 107
Size: 294 351 3371
Fragments arranged by size:
3371 351 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1259 566
Size: 693 3323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 906 941 906
Size: 35 3981
DraI TTT'AAA
Cuts at: 585 1015 585
Size: 430 3586
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 332 600 1601 1775 2166 2193 3538 332
Size: 268 1001 174 391 27 1345 810
Fragments arranged by size:
1345 1001 810 391 268 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1841 2273 3020 3288 862
Size: 979 432 747 268 1590
Fragments arranged by size:
1590 979 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1155 1155
Size: 4016
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
EcoRV GAT'ATC
Cuts at: 780 780
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 1450 1698 2720 3699 1450
Size: 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1858 1888 1890 1920 1858
Size: 30 2 30 3954
Fragments arranged by size:
3954 30 30 2
HincII GTy'rAC
Cuts at: 713 1055 713
Size: 342 3674
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 192 1036 192
Size: 844 3172
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 807 2687 2871 807
Size: 1880 184 1952
Fragments arranged by size:
1952 1880 184
MscI TGG'CCA
Cuts at: 334 1777 334
Size: 1443 2573
MslI CAynn'nnrTG
Cuts at: 637 757 2005 2132 637
Size: 120 1248 127 2521
Fragments arranged by size:
2521 1248 127 120
MspA1I CmG'CkG
Cuts at: 679 1127 1441 1801 2814 3059 679
Size: 448 314 360 1013 245 1636
Fragments arranged by size:
1636 1013 448 360 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 107
Size: 4016
NdeI CA'TA_TG
Cuts at: 706 706
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NheI G'CTAG_C
Cuts at: 256 256
Size: 4016
NsiI A_TGCA'T
Cuts at: 610 610
Size: 4016
NspI r_CATG'y
Cuts at: 385 876 1069 1569 2476 385
Size: 491 193 500 907 1925
Fragments arranged by size:
1925 907 500 491 193
PasI CC'CwG_GG
Cuts at: 243 243
Size: 4016
PciI A'CATG_T
Cuts at: 872 2472 872
Size: 1600 2416
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 208 240 208
Size: 32 3984
PstI C_TGCA'G
Cuts at: 120 1063 1748 120
Size: 943 685 2388
Fragments arranged by size:
2388 943 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 116 1059 1744 2737 2928 33
Size: 83 943 685 993 191 1121
Fragments arranged by size:
1121 993 943 685 191 83
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 264 412 1171 2086 2578 2840 264
Size: 148 759 915 492 262 1440
Fragments arranged by size:
1440 915 759 492 262 148
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 107 802 1150 1504 107
Size: 695 348 354 2619
Fragments arranged by size:
2619 695 354 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 649 1599 1732 2191 2400 649
Size: 950 133 459 209 2265
Fragments arranged by size:
2265 950 459 209 133
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 741 773 3533 3565 741
Size: 32 2760 32 1192
Fragments arranged by size:
2760 1192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 811 811
Size: 4016
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI