Restriction map for
pF1KE0464
(Circular) (Six-base) MAPSORT of: pF1KE0464.seq Check: 6303 from: 1 to: 3779
pF1KE0464 3779 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 612 817 612
Size: 205 3574
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 639 3619 639
Size: 2980 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1604 2036 2783 3051 1604
Size: 432 747 268 2332
Fragments arranged by size:
2332 747 432 268
AflII C'TTAA_G
Cuts at: 559 559
Size: 3779
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 2167 2167
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 1084 2651 1084
Size: 1567 2212
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 156 499 783 3132 3388 156
Size: 343 284 2349 256 547
Fragments arranged by size:
2349 547 343 284 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 799 799
Size: 3779
BamHI G'GATC_C
Cuts at: 804 804
Size: 3779
BanI G'GyrC_C
Cuts at: 795 1209 1457 1492 795
Size: 414 248 35 3082
Fragments arranged by size:
3082 414 248 35
BanII G_rGCy'C
Cuts at: 793 1823 793
Size: 1030 2749
BbeI G_GCGC'C
Cuts at: 1213 1461 1213
Size: 248 3531
BbsI GAAGACnn'nnnn_
Cuts at: 316 667 316
Size: 351 3428
BciVI GTATCCnnnnn_n'
Cuts at: 591 1677 2438 3779 591
Size: 1086 761 1341 591
Fragments arranged by size:
1341 1086 761 591
BclI T'GATC_A
Cuts at: 692 1299 692
Size: 607 3172
BglII A'GATC_T
Cuts at: 1294 3331 1294
Size: 2037 1742
BlpI GC'TnA_GC
Cuts at: 891 891
Size: 3779
Bme1580I G_kGCm'C
Cuts at: 1404 1497 2553 1404
Size: 93 1056 2630
Fragments arranged by size:
2630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 504 991 1072 1407 3551 504
Size: 487 81 335 2144 732
Fragments arranged by size:
2144 732 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 3779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 374
Size: 3779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 186 1132 186
Size: 946 2833
BsaBI GATnn'nnATC
Cuts at: 6 1293 3330 6
Size: 1287 2037 455
Fragments arranged by size:
2037 1287 455
BsaHI Gr'CG_yC
Cuts at: 1210 1458 2160 3656 1210
Size: 248 702 1496 1333
Fragments arranged by size:
1496 1333 702 248
BsaWI w'CCGG_w
Cuts at: 968 1489 2167 2441 2588 2999 968
Size: 521 678 274 147 411 1748
Fragments arranged by size:
1748 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1204 1962 2539 3035 1204
Size: 758 577 496 1948
Fragments arranged by size:
1948 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1321 1367 2575 104
Size: 1217 46 1208 1308
Fragments arranged by size:
1308 1217 1208 46
BsiHKAI G_wGCw'C
Cuts at: 333 793 1571 1761 2553 333
Size: 460 778 190 792 1559
Fragments arranged by size:
1559 792 778 460 190
BsmI GAATG_Cn'
Cuts at: 255 255
Size: 3779
Bsp1286I G_dGCh'C
Cuts at: 333 793 1404 1497 1571 1761 1823 2553 333
Size: 460 611 93 74 190 62 730 1559
Fragments arranged by size:
1559 730 611 460 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1043 1345 1726 829
Size: 214 302 381 2882
Fragments arranged by size:
2882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2071 2125 3609 3774 50
Size: 2021 54 1484 165 55
Fragments arranged by size:
2021 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 236 1691 236
Size: 1455 2324
BsrFI r'CCGG_y
Cuts at: 256 1777 1958 2167 256
Size: 1521 181 209 1868
Fragments arranged by size:
1868 1521 209 181
BssSI C'ACGA_G
Cuts at: 2050 2408 2050
Size: 358 3421
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1156
Size: 3779
BstBI TT'CG_AA
Cuts at: 160 2140 160
Size: 1980 1799
BstXI CCAn_nnnn'nTGG
Cuts at: 475 475
Size: 3779
BstYI r'GATC_y
Cuts at: 1 804 1271 1294 1629 1875 2887 2973 2985
Size: 803 467 23 335 246 1012 86 12
Cuts at: 2985 3331 1
Size: 346 449
Fragments arranged by size:
1012 803 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1729 1910 2073 1729
Size: 181 163 3435
Fragments arranged by size:
3435 181 163
BtsI GCAGTG_nn'
Cuts at: 1022 1022
Size: 3779
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 1364 1538 1929 1956 3301 1364
Size: 174 391 27 1345 1842
Fragments arranged by size:
1842 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1364 1364
Size: 3779
EarI CTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 1987 2297 2443 3589 114
Size: 1873 310 146 1146 304
Fragments arranged by size:
1873 1146 310 304 146
EcoICRI GAG'CTC
Cuts at: 791 791
Size: 3779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1604 2036 2783 3051 374
Size: 1230 432 747 268 1102
Fragments arranged by size:
1230 1102 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 275 275
Size: 3779
EcoO109I rG'GnC_Cy
Cuts at: 918 918
Size: 3779
EcoRI G'AATT_C
Cuts at: 499 783 499
Size: 284 3495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3779
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 1213 1461 2483 3462 1213
Size: 248 1022 979 1530
Fragments arranged by size:
1530 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1621 1651 1653 1683 1621
Size: 30 2 30 3717
Fragments arranged by size:
3717 30 30 2
HincII GTy'rAC
Cuts at: 818 818
Size: 3779
KasI G'GCGC_C
Cuts at: 1209 1457 1209
Size: 248 3531
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2634 2450
Size: 184 3595
MscI TGG'CCA
Cuts at: 1540 1540
Size: 3779
MslI CAynn'nnrTG
Cuts at: 1768 1895 1768
Size: 127 3652
MspA1I CmG'CkG
Cuts at: 890 1204 1564 2577 2822 890
Size: 314 360 1013 245 1847
Fragments arranged by size:
1847 1013 360 314 245
NaeI GCC'GGC
Cuts at: 258 1960 258
Size: 1702 2077
NarI GG'CG_CC
Cuts at: 1210 1458 1210
Size: 248 3531
NcoI C'CATG_G
Cuts at: 107 107
Size: 3779
NgoMIV G'CCGG_C
Cuts at: 256 1958 256
Size: 1702 2077
NspI r_CATG'y
Cuts at: 832 1332 2239 832
Size: 500 907 2372
Fragments arranged by size:
2372 907 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PshAI GACnn'nnGTC
Cuts at: 740 740
Size: 3779
PstI C_TGCA'G
Cuts at: 826 1511 826
Size: 685 3094
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 1204 1564 1204
Size: 360 3419
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 793 793
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SfcI C'TryA_G
Cuts at: 33 391 531 822 1507 2500 2691 33
Size: 358 140 291 685 993 191 1121
Fragments arranged by size:
1121 993 685 358 291 191 140
SfoI GGC'GCC
Cuts at: 1211 1459 1211
Size: 248 3531
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 801 801
Size: 3779
SmlI C'TyrA_G
Cuts at: 559 934 1849 2341 2603 559
Size: 375 915 492 262 1735
Fragments arranged by size:
1735 915 492 375 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
SspI AAT'ATT
Cuts at: 339 339
Size: 3779
StyI C'CwwG_G
Cuts at: 107 913 1267 107
Size: 806 354 2619
Fragments arranged by size:
2619 806 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 644 1362 1495 1954 2163 644
Size: 718 133 459 209 2260
Fragments arranged by size:
2260 718 459 209 133
TatI w'GTAC_w
Cuts at: 3294 3294
Size: 3779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 810 810
Size: 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1274 1274
Size: 3779
XmaI C'CCGG_G
Cuts at: 799 799
Size: 3779
XmnI GAAnn'nnTTC
Cuts at: 410 410
Size: 3779
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI