Restriction map for
pF1KE0452
(Circular) (Six-base) MAPSORT of: pF1KE0452.seq Check: 6150 from: 1 to: 4499
pF1KE0452 4499 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 671 720 1515 671
Size: 49 795 3655
Fragments arranged by size:
3655 795 49
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2324 2756 3503 3771 1261
Size: 1063 432 747 268 1989
Fragments arranged by size:
1989 1063 747 432 268
AflIII A'CryG_T
Cuts at: 417 806 1851 2955 3932 417
Size: 389 1045 1104 977 984
Fragments arranged by size:
1104 1045 984 977 389
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaI G_GGCC'C
Cuts at: 1180 1180
Size: 4499
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 279 330 1064 1503 3852 4108 279
Size: 51 734 439 2349 256 670
Fragments arranged by size:
2349 734 670 439 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 596 1519 596
Size: 923 3576
BamHI G'GATC_C
Cuts at: 600 1524 600
Size: 924 3575
BanI G'GyrC_C
Cuts at: 671 720 1515 1929 2177 2212 671
Size: 49 795 414 248 35 2958
Fragments arranged by size:
2958 795 414 248 49 35
BanII G_rGCy'C
Cuts at: 222 1180 1513 2543 222
Size: 958 333 1030 2178
Fragments arranged by size:
2178 1030 958 333
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 883 883
Size: 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1207 1241 1207
Size: 34 4465
BciVI GTATCCnnnnn_n'
Cuts at: 1177 2397 3158 4499 1177
Size: 1220 761 1341 1177
Fragments arranged by size:
1341 1220 1177 761
BclI T'GATC_A
Cuts at: 798 2019 798
Size: 1221 3278
BfrBI ATG'CAT
Cuts at: 1468 1468
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 639 1611 639
Size: 972 3527
Bme1580I G_kGCm'C
Cuts at: 1180 2124 2217 3273 1180
Size: 944 93 1056 2406
Fragments arranged by size:
2406 1056 944 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BmtI G_CTAG'C
Cuts at: 1127 1127
Size: 4499
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 546 530
Size: 16 4483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1675 2554 3046 3344 1675
Size: 879 492 298 2830
Fragments arranged by size:
2830 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 189 971 4203 189
Size: 782 3232 485
Fragments arranged by size:
3232 782 485
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 544
Size: 30 4469
BseYI C'CCAG_C
Cuts at: 1288 1924 2682 3259 3755 1288
Size: 636 758 577 496 2032
Fragments arranged by size:
2032 758 636 577 496
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 222 1513 2291 2481 3273 222
Size: 1291 778 190 792 1448
Fragments arranged by size:
1448 1291 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 222 1180 1513 2124 2217 2291 2481 2543 3273
Size: 958 333 611 93 74 190 62 730
Cuts at: 3273 222
Size: 1448
Fragments arranged by size:
1448 958 730 611 333 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 891 2411 891
Size: 1520 2979
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BsrGI T'GTAC_A
Cuts at: 809 809
Size: 4499
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1876 1465
Size: 411 4088
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstXI CCAn_nnnn'nTGG
Cuts at: 732 732
Size: 4499
BstYI r'GATC_y
Cuts at: 1 600 1524 1991 2014 2349 2595 3607 3693
Size: 599 924 467 23 335 246 1012 86
Cuts at: 3693 3705 4051 1
Size: 12 346 449
Fragments arranged by size:
1012 924 599 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 960 960
Size: 4499
BtgI C'CryG_G
Cuts at: 107 1207 107
Size: 1100 3399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
DraI TTT'AAA
Cuts at: 185 335 1498 185
Size: 150 1163 3186
Fragments arranged by size:
3186 1163 150
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 2084 2258 2649 2676 4021 2084
Size: 174 391 27 1345 2562
Fragments arranged by size:
2562 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 896 2522 2732 896
Size: 1626 210 2663
Fragments arranged by size:
2663 1626 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 220 1511 220
Size: 1291 3208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 546 1261 2324 2756 3503 3771 530
Size: 16 715 1063 432 747 268 1258
Fragments arranged by size:
1258 1063 747 715 432 268 16
EcoNI CCTnn'n_nnAGG
Cuts at: 861 861
Size: 4499
EcoO109I rG'GnC_Cy
Cuts at: 501 1638 501
Size: 1137 3362
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1478 2518 1478
Size: 1040 3459
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 2341 2371 2373 2403 544
Size: 32 1765 30 2 30 2640
Fragments arranged by size:
2640 1765 32 30 30 2
HincII GTy'rAC
Cuts at: 953 1538 953
Size: 585 3914
HpaI GTT'AAC
Cuts at: 953 953
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 675 724 1519 675
Size: 49 795 3655
Fragments arranged by size:
3655 795 49
MfeI C'AATT_G
Cuts at: 647 647
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3354 3170
Size: 184 4315
MscI TGG'CCA
Cuts at: 2260 2260
Size: 4499
MslI CAynn'nnrTG
Cuts at: 1034 2488 2615 1034
Size: 1454 127 2918
Fragments arranged by size:
2918 1454 127
MspA1I CmG'CkG
Cuts at: 1482 1610 1924 2284 3297 3542 1482
Size: 128 314 360 1013 245 2439
Fragments arranged by size:
2439 1013 360 314 245 128
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 107 1207 107
Size: 1100 3399
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NheI G'CTAG_C
Cuts at: 1123 1123
Size: 4499
NsiI A_TGCA'T
Cuts at: 1470 1470
Size: 4499
NspI r_CATG'y
Cuts at: 421 810 1552 2052 2959 421
Size: 389 742 500 907 1961
Fragments arranged by size:
1961 907 742 500 389
PciI A'CATG_T
Cuts at: 417 806 2955 417
Size: 389 2149 1961
Fragments arranged by size:
2149 1961 389
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 501 501
Size: 4499
PspOMI G'GGCC_C
Cuts at: 1176 1176
Size: 4499
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1353 1385 1353
Size: 32 4467
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1482 1924 2284 1482
Size: 442 360 3697
Fragments arranged by size:
3697 442 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 222 1513 222
Size: 1291 3208
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SanDI GG'GwC_CC
Cuts at: 501 501
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 896 2522 2732 896
Size: 1626 210 2663
Fragments arranged by size:
2663 1626 210
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SexAI A'CCwGG_T
Cuts at: 1401 1401
Size: 4499
SfcI C'TryA_G
Cuts at: 33 1542 2227 3220 3411 33
Size: 1509 685 993 191 1121
Fragments arranged by size:
1509 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 1654 2569 3061 3323 1654
Size: 915 492 262 2830
Fragments arranged by size:
2830 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
SspI AAT'ATT
Cuts at: 1160 1160
Size: 4499
StyI C'CwwG_G
Cuts at: 107 1207 1633 1987 107
Size: 1100 426 354 2619
Fragments arranged by size:
2619 1100 426 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2082 2215 2674 2883 2082
Size: 133 459 209 3698
Fragments arranged by size:
3698 459 209 133
TatI w'GTAC_w
Cuts at: 654 809 4014 654
Size: 155 3205 1139
Fragments arranged by size:
3205 1139 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BglI BmgBI Bpu10I BseRI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII NdeI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI SacII SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI