Restriction map for
pF1KE0448
(Circular) (Six-base) MAPSORT of: pF1KE0448.seq Check: 4144 from: 1 to: 3584
pF1KE0448 3584 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 622 622
Size: 3584
Acc65I G'GTAC_C
Cuts at: 141 170 600 141
Size: 29 430 3125
Fragments arranged by size:
3125 430 29
AclI AA'CG_TT
Cuts at: 3424 3424
Size: 3584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1409 1841 2588 2856 498
Size: 911 432 747 268 1226
Fragments arranged by size:
1226 911 747 432 268
AflIII A'CryG_T
Cuts at: 936 2040 3017 936
Size: 1104 977 1503
Fragments arranged by size:
1503 1104 977
AgeI A'CCGG_T
Cuts at: 1972 1972
Size: 3584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 175
Size: 32 3552
AlwNI CAG_nnn'CTG
Cuts at: 889 2456 889
Size: 1567 2017
ApaLI G'TGCA_C
Cuts at: 2354 2354
Size: 3584
ApoI r'AATT_y
Cuts at: 588 2937 3193 588
Size: 2349 256 979
Fragments arranged by size:
2349 979 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3584
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3584
AvaI C'yCGr_G
Cuts at: 604 604
Size: 3584
BamHI G'GATC_C
Cuts at: 438 609 438
Size: 171 3413
BanI G'GyrC_C
Cuts at: 141 170 207 600 1014 1262 1297 141
Size: 29 37 393 414 248 35 2428
Fragments arranged by size:
2428 414 393 248 37 35 29
BanII G_rGCy'C
Cuts at: 598 1628 598
Size: 1030 2554
BbeI G_GCGC'C
Cuts at: 1018 1266 1018
Size: 248 3336
BciVI GTATCCnnnnn_n'
Cuts at: 1482 2243 3584 1482
Size: 761 1341 1482
Fragments arranged by size:
1482 1341 761
BclI T'GATC_A
Cuts at: 1104 1104
Size: 3584
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3584
BglII A'GATC_T
Cuts at: 1099 3136 1099
Size: 2037 1547
BlpI GC'TnA_GC
Cuts at: 696 696
Size: 3584
Bme1580I G_kGCm'C
Cuts at: 1209 1302 2358 1209
Size: 93 1056 2435
Fragments arranged by size:
2435 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 796 877 1212 3356 796
Size: 81 335 2144 1024
Fragments arranged by size:
2144 1024 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1951 1951
Size: 3584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 232
Size: 3584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 592 760 1639 2131 2429 277
Size: 315 168 879 492 298 1432
Fragments arranged by size:
1432 879 492 315 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 3288 3288
Size: 3584
BsaAI yAC'GTr
Cuts at: 937 937
Size: 3584
BsaBI GATnn'nnATC
Cuts at: 6 1098 3135 6
Size: 1092 2037 455
Fragments arranged by size:
2037 1092 455
BsaHI Gr'CG_yC
Cuts at: 1015 1263 1965 3461 1015
Size: 248 702 1496 1138
Fragments arranged by size:
1496 1138 702 248
BsaWI w'CCGG_w
Cuts at: 773 1294 1972 2246 2393 2804 773
Size: 521 678 274 147 411 1553
Fragments arranged by size:
1553 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 175
Size: 30 3554
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 199 139
Size: 60 3524
BseYI C'CCAG_C
Cuts at: 1009 1767 2344 2840 1009
Size: 758 577 496 1753
Fragments arranged by size:
1753 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 282 1126 1172 2380 104
Size: 178 844 46 1208 1308
Fragments arranged by size:
1308 1208 844 178 46
BsiHKAI G_wGCw'C
Cuts at: 598 1376 1566 2358 598
Size: 778 190 792 1824
Fragments arranged by size:
1824 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 598 1209 1302 1376 1566 1628 2358 598
Size: 611 93 74 190 62 730 1824
Fragments arranged by size:
1824 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2804 2804
Size: 3584
BspHI T'CATG_A
Cuts at: 2760 2760
Size: 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 634 848 1150 1531 634
Size: 214 302 381 2687
Fragments arranged by size:
2687 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 1876 1930 3414 3579 50
Size: 79 1747 54 1484 165 55
Fragments arranged by size:
1747 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 1496 1496
Size: 3584
BsrFI r'CCGG_y
Cuts at: 254 1582 1763 1972 254
Size: 1328 181 209 1866
Fragments arranged by size:
1866 1328 209 181
BssHII G'CGCG_C
Cuts at: 222 222
Size: 3584
BssSI C'ACGA_G
Cuts at: 1855 2213 1855
Size: 358 3226
BstAPI GCAn_nnn'nTGC
Cuts at: 961 961
Size: 3584
BstBI TT'CG_AA
Cuts at: 1945 1945
Size: 3584
BstYI r'GATC_y
Cuts at: 1 287 438 609 1076 1099 1434 1680 2692
Size: 286 151 171 467 23 335 246 1012
Cuts at: 2692 2778 2790 3136 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 286 246 171
151 86 23 12
BtgI C'CryG_G
Cuts at: 157 157
Size: 3584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1534 1715 1878 1534
Size: 181 163 3240
Fragments arranged by size:
3240 181 163
BtsI GCAGTG_nn'
Cuts at: 827 827
Size: 3584
DraI TTT'AAA
Cuts at: 583 583
Size: 3584
DrdI GACnn_nn'nnGTC
Cuts at: 1290 2148 1290
Size: 858 2726
EaeI y'GGCC_r
Cuts at: 279 1169 1343 1734 1761 3106 279
Size: 890 174 391 27 1345 757
Fragments arranged by size:
1345 890 757 391 174 27
EagI C'GGCC_G
Cuts at: 279 1169 279
Size: 890 2694
EarI CTCTTCn'nnn_
Cuts at: 1607 1817 1607
Size: 210 3374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1792 2102 2248 3394 1792
Size: 310 146 1146 1982
Fragments arranged by size:
1982 1146 310 146
EcoICRI GAG'CTC
Cuts at: 596 596
Size: 3584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 498 1409 1841 2588 2856 232
Size: 266 911 432 747 268 960
Fragments arranged by size:
960 911 747 432 268 266
EcoO109I rG'GnC_Cy
Cuts at: 152 723 152
Size: 571 3013
EcoRI G'AATT_C
Cuts at: 588 588
Size: 3584
EcoRV GAT'ATC
Cuts at: 234 234
Size: 3584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1603 1603
Size: 3584
FspI TGC'GCA
Cuts at: 1365 1365
Size: 3584
HaeII r_GCGC'y
Cuts at: 268 1018 1266 2288 3267 268
Size: 750 248 1022 979 585
Fragments arranged by size:
1022 979 750 585 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 512 544 1426 1456 1458 1488 512
Size: 32 882 30 2 30 2608
Fragments arranged by size:
2608 882 32 30 30 2
HincII GTy'rAC
Cuts at: 623 623
Size: 3584
HindIII A'AGCT_T
Cuts at: 469 469
Size: 3584
KasI G'GCGC_C
Cuts at: 1014 1262 1014
Size: 248 3336
KpnI G_GTAC'C
Cuts at: 145 174 604 145
Size: 29 430 3125
Fragments arranged by size:
3125 430 29
MluI A'CGCG_T
Cuts at: 3017 3017
Size: 3584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 385 2255 2439 385
Size: 1870 184 1530
Fragments arranged by size:
1870 1530 184
MscI TGG'CCA
Cuts at: 1345 1345
Size: 3584
MslI CAynn'nnrTG
Cuts at: 1573 1700 1573
Size: 127 3457
MspA1I CmG'CkG
Cuts at: 436 695 1009 1369 2382 2627 436
Size: 259 314 360 1013 245 1393
Fragments arranged by size:
1393 1013 360 314 259 245
NaeI GCC'GGC
Cuts at: 256 1765 256
Size: 1509 2075
NarI GG'CG_CC
Cuts at: 1015 1263 1015
Size: 248 3336
NgoMIV G'CCGG_C
Cuts at: 254 1763 254
Size: 1509 2075
NotI GC'GGCC_GC
Cuts at: 279 279
Size: 3584
NspI r_CATG'y
Cuts at: 262 637 1137 2044 262
Size: 375 500 907 1802
Fragments arranged by size:
1802 907 500 375
PciI A'CATG_T
Cuts at: 2040 2040
Size: 3584
PflMI CCAn_nnn'nTGG
Cuts at: 180 180
Size: 3584
PmeI GTTT'AAAC
Cuts at: 583 583
Size: 3584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 175 207 2723 2755 175
Size: 32 2516 32 1004
Fragments arranged by size:
2516 1004 32 32
PpuMI rG'GwC_Cy
Cuts at: 152 152
Size: 3584
PstI C_TGCA'G
Cuts at: 631 1316 631
Size: 685 2899
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3584
PvuII CAG'CTG
Cuts at: 1009 1369 1009
Size: 360 3224
RsrII CG'GwC_CG
Cuts at: 1779 1779
Size: 3584
SacI G_AGCT'C
Cuts at: 598 598
Size: 3584
SalI G'TCGA_C
Cuts at: 621 621
Size: 3584
SanDI GG'GwC_CC
Cuts at: 152 152
Size: 3584
SapI GCTCTTCn'nnn_
Cuts at: 1607 1817 1607
Size: 210 3374
SbfI CC_TGCA'GG
Cuts at: 631 631
Size: 3584
ScaI AGT'ACT
Cuts at: 3101 3101
Size: 3584
SfcI C'TryA_G
Cuts at: 33 627 1312 2305 2496 33
Size: 594 685 993 191 1121
Fragments arranged by size:
1121 993 685 594 191
SfoI GGC'GCC
Cuts at: 1016 1264 1016
Size: 248 3336
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3584
SmaI CCC'GGG
Cuts at: 606 606
Size: 3584
SmlI C'TyrA_G
Cuts at: 292 571 739 1654 2146 2408 292
Size: 279 168 915 492 262 1468
Fragments arranged by size:
1468 915 492 279 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3584
SphI G_CATG'C
Cuts at: 637 1137 637
Size: 500 3084
StyI C'CwwG_G
Cuts at: 179 442 718 1072 179
Size: 263 276 354 2691
Fragments arranged by size:
2691 354 276 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 435 1167 1300 1759 1968 435
Size: 732 133 459 209 2051
Fragments arranged by size:
2051 732 459 209 133
TatI w'GTAC_w
Cuts at: 3099 3099
Size: 3584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 223 255 3101 3133 223
Size: 32 2846 32 674
Fragments arranged by size:
2846 674 32 32
Tth111I GACn'n_nGTC
Cuts at: 1381 1381
Size: 3584
XbaI T'CTAG_A
Cuts at: 615 615
Size: 3584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1079 1079
Size: 3584
XmaI C'CCGG_G
Cuts at: 604 604
Size: 3584
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NheI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI