Restriction map for
pF1KE0446
(Circular) (Six-base) MAPSORT of: pF1KE0446.seq Check: 2884 from: 1 to: 3824
pF1KE0446 3824 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 461 653 1649 2081 2828 3096 137
Size: 324 192 996 432 747 268 865
Fragments arranged by size:
996 865 747 432 324 268 192
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AlwNI CAG_nnn'CTG
Cuts at: 526 1129 2696 526
Size: 603 1567 1654
Fragments arranged by size:
1654 1567 603
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 256 828 3177 3433 256
Size: 572 2349 256 647
Fragments arranged by size:
2349 647 572 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 844 844
Size: 3824
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 840 1254 1502 1537 840
Size: 414 248 35 3127
Fragments arranged by size:
3127 414 248 35
BanII G_rGCy'C
Cuts at: 337 838 1868 337
Size: 501 1030 2293
Fragments arranged by size:
2293 1030 501
BbeI G_GCGC'C
Cuts at: 1258 1506 1258
Size: 248 3576
BbsI GAAGACnn'nnnn_
Cuts at: 143 143
Size: 3824
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 936 936
Size: 3824
Bme1580I G_kGCm'C
Cuts at: 1449 1542 2598 1449
Size: 93 1056 2675
Fragments arranged by size:
2675 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1036 1117 1452 3596 1036
Size: 81 335 2144 1264
Fragments arranged by size:
2144 1264 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2371 2669 1000
Size: 879 492 298 2155
Fragments arranged by size:
2155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 3824
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 1255 1503 2205 3701 1255
Size: 248 702 1496 1378
Fragments arranged by size:
1496 1378 702 248
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2486 2633 3044 1013
Size: 521 678 274 147 411 1793
Fragments arranged by size:
1793 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 301
Size: 30 3794
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 697
Size: 3824
BseYI C'CCAG_C
Cuts at: 1249 2007 2584 3080 1249
Size: 758 577 496 1993
Fragments arranged by size:
1993 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1366 1412 2620 104
Size: 1262 46 1208 1308
Fragments arranged by size:
1308 1262 1208 46
BsiHKAI G_wGCw'C
Cuts at: 337 838 1616 1806 2598 337
Size: 501 778 190 792 1563
Fragments arranged by size:
1563 792 778 501 190
Bsp1286I G_dGCh'C
Cuts at: 337 838 1449 1542 1616 1806 1868 2598 337
Size: 501 611 93 74 190 62 730 1563
Fragments arranged by size:
1563 730 611 501 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 874 1088 1390 1771 874
Size: 214 302 381 2927
Fragments arranged by size:
2927 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2116 2170 3654 3819 50
Size: 2066 54 1484 165 55
Fragments arranged by size:
2066 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 1736 128
Size: 1608 2216
BsrFI r'CCGG_y
Cuts at: 1822 2003 2212 1822
Size: 181 209 3434
Fragments arranged by size:
3434 209 181
BssSI C'ACGA_G
Cuts at: 2095 2453 2095
Size: 358 3466
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1201
Size: 3824
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstXI CCAn_nnnn'nTGG
Cuts at: 249 249
Size: 3824
BstYI r'GATC_y
Cuts at: 1 849 1316 1339 1674 1920 2932 3018 3030
Size: 848 467 23 335 246 1012 86 12
Cuts at: 3030 3376 1
Size: 346 449
Fragments arranged by size:
1012 848 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 717 1067 717
Size: 350 3474
DraI TTT'AAA
Cuts at: 551 823 551
Size: 272 3552
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2388 1530
Size: 858 2966
EaeI y'GGCC_r
Cuts at: 1409 1583 1974 2001 3346 1409
Size: 174 391 27 1345 1887
Fragments arranged by size:
1887 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1409 1409
Size: 3824
EarI CTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2342 2488 3634 2032
Size: 310 146 1146 2222
Fragments arranged by size:
2222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 335 836 335
Size: 501 3323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 461 653 1649 2081 2828 3096 137
Size: 324 192 996 432 747 268 865
Fragments arranged by size:
996 865 747 432 324 268 192
EcoO109I rG'GnC_Cy
Cuts at: 963 963
Size: 3824
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 1258 1506 2528 3507 1258
Size: 248 1022 979 1575
Fragments arranged by size:
1575 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 646 678 1666 1696 1698 1728 322
Size: 32 292 32 988 30 2 30 2418
Fragments arranged by size:
2418 988 292 32 32 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 1254 1502 1254
Size: 248 3576
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2679 2495
Size: 184 3640
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 1813 1940 1813
Size: 127 3697
MspA1I CmG'CkG
Cuts at: 935 1249 1609 2622 2867 935
Size: 314 360 1013 245 1892
Fragments arranged by size:
1892 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2005 2005
Size: 3824
NarI GG'CG_CC
Cuts at: 1255 1503 1255
Size: 248 3576
NgoMIV G'CCGG_C
Cuts at: 2003 2003
Size: 3824
NspI r_CATG'y
Cuts at: 877 1377 2284 877
Size: 500 907 2417
Fragments arranged by size:
2417 907 500
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PstI C_TGCA'G
Cuts at: 871 1556 871
Size: 685 3139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 1249 1609 1249
Size: 360 3464
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 337 838 337
Size: 501 3323
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 867 1552 2545 2736 33
Size: 834 685 993 191 1121
Fragments arranged by size:
1121 993 834 685 191
SfoI GGC'GCC
Cuts at: 1256 1504 1256
Size: 248 3576
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 846 846
Size: 3824
SmlI C'TyrA_G
Cuts at: 979 1894 2386 2648 979
Size: 915 492 262 2155
Fragments arranged by size:
2155 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StuI AGG'CCT
Cuts at: 192 192
Size: 3824
StyI C'CwwG_G
Cuts at: 187 242 958 1312 187
Size: 55 716 354 2699
Fragments arranged by size:
2699 716 354 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 1540 1999 2208 1407
Size: 133 459 209 3023
Fragments arranged by size:
3023 459 209 133
TatI w'GTAC_w
Cuts at: 3339 3339
Size: 3824
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 194 1319 194
Size: 1125 2699
XmaI C'CCGG_G
Cuts at: 844 844
Size: 3824
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI