Restriction map for
pF1KE0442
(Circular) (Six-base) MAPSORT of: pF1KE0442.seq Check: 3927 from: 1 to: 4082
pF1KE0442 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 400 555 559 1057 1907 2339 3086 3354
Size: 183 155 4 498 850 432 747 268
Cuts at: 3354 217
Size: 945
Fragments arranged by size:
945 850 747 498 432 268 183 155
4
AflIII A'CryG_T
Cuts at: 1434 2538 3515 1434
Size: 1104 977 2001
Fragments arranged by size:
2001 1104 977
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 486 1387 2954 486
Size: 901 1567 1614
Fragments arranged by size:
1614 1567 901
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 506 1086 3435 3691 506
Size: 580 2349 256 897
Fragments arranged by size:
2349 897 580 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 1102 1102
Size: 4082
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 1096 2126 1096
Size: 1030 3052
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BbvCI CC'TCA_GC
Cuts at: 775 775
Size: 4082
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 182 1194 182
Size: 1012 3070
Bme1580I G_kGCm'C
Cuts at: 358 1707 1800 2856 358
Size: 1349 93 1056 1584
Fragments arranged by size:
1584 1349 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
Bpu10I CC'TnA_GC
Cuts at: 706 775 706
Size: 69 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2137 2629 2927 1258
Size: 879 492 298 2413
Fragments arranged by size:
2413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 3786 841
Size: 2945 1137
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 1513 1761 2463 3959 1513
Size: 248 702 1496 1636
Fragments arranged by size:
1636 1496 702 248
BsaWI w'CCGG_w
Cuts at: 816 1271 1792 2470 2744 2891 3302 816
Size: 455 521 678 274 147 411 1596
Fragments arranged by size:
1596 678 521 455 411 274 147
BseYI C'CCAG_C
Cuts at: 1507 2265 2842 3338 1507
Size: 758 577 496 2251
Fragments arranged by size:
2251 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
BsmI GAATG_Cn'
Cuts at: 351 351
Size: 4082
Bsp1286I G_dGCh'C
Cuts at: 358 1096 1707 1800 1874 2064 2126 2856 358
Size: 738 611 93 74 190 62 730 1584
Fragments arranged by size:
1584 738 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 816 3302 816
Size: 2486 1596
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 125 269 1132 1346 1648 2029 125
Size: 144 863 214 302 381 2178
Fragments arranged by size:
2178 863 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 1073 1994 717
Size: 356 921 2805
Fragments arranged by size:
2805 921 356
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstYI r'GATC_y
Cuts at: 1 290 1107 1574 1597 1932 2178 3190 3276
Size: 289 817 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 817 467 449 346 335 289 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1012 1012
Size: 4082
BtgI C'CryG_G
Cuts at: 107 107
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
ClaI AT'CG_AT
Cuts at: 941 941
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DraIII CAC_nnn'GTG
Cuts at: 912 912
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 653 1667 1841 2232 2259 3604 653
Size: 1014 174 391 27 1345 1131
Fragments arranged by size:
1345 1131 1014 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 419 676 839 2105 2315 419
Size: 257 163 1266 210 2186
Fragments arranged by size:
2186 1266 257 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 400 555 559 1057 1907 2339 3086 3354
Size: 183 155 4 498 850 432 747 268
Cuts at: 3354 217
Size: 945
Fragments arranged by size:
945 850 747 498 432 268 183 155
4
EcoNI CCTnn'n_nnAGG
Cuts at: 191 490 191
Size: 299 3783
EcoO109I rG'GnC_Cy
Cuts at: 483 978 1221 483
Size: 495 243 3344
Fragments arranged by size:
3344 495 243
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 227 1516 1764 2786 3765 227
Size: 1289 248 1022 979 544
Fragments arranged by size:
1289 1022 979 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 872 904 1924 1954 1956 1986 642
Size: 32 198 32 1020 30 2 30 2738
Fragments arranged by size:
2738 1020 198 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
HindIII A'AGCT_T
Cuts at: 338 338
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2937 2753
Size: 184 3898
MscI TGG'CCA
Cuts at: 655 1843 655
Size: 1188 2894
MslI CAynn'nnrTG
Cuts at: 691 868 2071 2198 691
Size: 177 1203 127 2575
Fragments arranged by size:
2575 1203 177 127
MspA1I CmG'CkG
Cuts at: 1193 1507 1867 2880 3125 1193
Size: 314 360 1013 245 2150
Fragments arranged by size:
2150 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 618 1135 1635 2542 618
Size: 517 500 907 2158
Fragments arranged by size:
2158 907 517 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PflMI CCAn_nnn'nTGG
Cuts at: 468 912 468
Size: 444 3638
PfoI T'CCnGG_A
Cuts at: 164 164
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PshAI GACnn'nnGTC
Cuts at: 790 790
Size: 4082
PstI C_TGCA'G
Cuts at: 266 1129 1814 266
Size: 863 685 2534
Fragments arranged by size:
2534 863 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 1507 1867 1507
Size: 360 3722
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 419 2105 2315 419
Size: 1686 210 2186
Fragments arranged by size:
2186 1686 210
SbfI CC_TGCA'GG
Cuts at: 266 1129 266
Size: 863 3219
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 188 262 579 1125 1810 2803 2994 33
Size: 155 74 317 546 685 993 191 1121
Fragments arranged by size:
1121 993 685 546 317 191 155 74
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 1237 2152 2644 2906 1237
Size: 915 492 262 2413
Fragments arranged by size:
2413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StyI C'CwwG_G
Cuts at: 107 1216 1570 107
Size: 1109 354 2619
Fragments arranged by size:
2619 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 1798 2257 2466 1665
Size: 133 459 209 3281
Fragments arranged by size:
3281 459 209 133
TatI w'GTAC_w
Cuts at: 821 3597 821
Size: 2776 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 3599 3631 146
Size: 32 3421 32 597
Fragments arranged by size:
3421 597 32 32
Tth111I GACn'n_nGTC
Cuts at: 901 1879 901
Size: 978 3104
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
XmnI GAAnn'nnTTC
Cuts at: 568 568
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI