Restriction map for
pF1KE0435
(Circular) (Six-base) MAPSORT of: pF1KE0435.seq Check: 9883 from: 1 to: 3884
pF1KE0435 3884 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 704 704
Size: 3884
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
AfeI AGC'GCT
Cuts at: 871 871
Size: 3884
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 1189 2756 1189
Size: 1567 2317
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 904 904
Size: 3884
BamHI G'GATC_C
Cuts at: 909 909
Size: 3884
BanI G'GyrC_C
Cuts at: 778 900 1314 1562 1597 778
Size: 122 414 248 35 3065
Fragments arranged by size:
3065 414 248 122 35
BanII G_rGCy'C
Cuts at: 898 1928 898
Size: 1030 2854
BbeI G_GCGC'C
Cuts at: 1318 1566 1318
Size: 248 3636
BbsI GAAGACnn'nnnn_
Cuts at: 165 165
Size: 3884
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 781 1509 1602 2658 781
Size: 728 93 1056 2007
Fragments arranged by size:
2007 1056 728 93
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 858
Size: 3884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 748 1315 1563 2265 3761 748
Size: 567 248 702 1496 871
Fragments arranged by size:
1496 871 702 567 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 856 886 323
Size: 30 503 30 3321
Fragments arranged by size:
3321 503 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 554 554
Size: 3884
BseYI C'CCAG_C
Cuts at: 1309 2067 2644 3140 1309
Size: 758 577 496 2053
Fragments arranged by size:
2053 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 119 1426 1472 2680 104
Size: 15 1307 46 1208 1308
Fragments arranged by size:
1308 1307 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 773 898 1676 1866 2658 773
Size: 125 778 190 792 1999
Fragments arranged by size:
1999 792 778 190 125
BsmI GAATG_Cn'
Cuts at: 548 787 548
Size: 239 3645
Bsp1286I G_dGCh'C
Cuts at: 773 781 898 1509 1602 1676 1866 1928 2658
Size: 8 117 611 93 74 190 62 730
Cuts at: 2658 773
Size: 1999
Fragments arranged by size:
1999 730 611 190 117 93 74 62
8
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 485 590 3060 485
Size: 105 2470 1309
Fragments arranged by size:
2470 1309 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 704 934 1148 1450 1831 704
Size: 230 214 302 381 2757
Fragments arranged by size:
2757 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 2176 2230 3714 3879 50
Size: 2126 54 1484 165 55
Fragments arranged by size:
2126 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 140 1796 140
Size: 1656 2228
BsrFI r'CCGG_y
Cuts at: 1882 2063 2272 1882
Size: 181 209 3494
Fragments arranged by size:
3494 209 181
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstYI r'GATC_y
Cuts at: 1 909 1376 1399 1734 1980 2992 3078 3090
Size: 908 467 23 335 246 1012 86 12
Cuts at: 3090 3436 1
Size: 346 449
Fragments arranged by size:
1012 908 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 165 198 1834 2015 2178 165
Size: 33 1636 181 163 1871
Fragments arranged by size:
1871 1636 181 163 33
BtsI GCAGTG_nn'
Cuts at: 1127 1127
Size: 3884
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2448 1590
Size: 858 3026
EaeI y'GGCC_r
Cuts at: 116 1469 1643 2034 2061 3406 116
Size: 1353 174 391 27 1345 594
Fragments arranged by size:
1353 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 116 1469 116
Size: 1353 2531
EarI CTCTTCn'nnn_
Cuts at: 406 1907 2117 406
Size: 1501 210 2173
Fragments arranged by size:
2173 1501 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2402 2548 3694 2092
Size: 310 146 1146 2282
Fragments arranged by size:
2282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 1709 2141 2888 3156 858
Size: 851 432 747 268 1586
Fragments arranged by size:
1586 851 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1023 1023
Size: 3884
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 873 1318 1566 2588 3567 873
Size: 445 248 1022 979 1190
Fragments arranged by size:
1190 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 323 355 1726 1756 1758 1788 323
Size: 32 1371 30 2 30 2419
Fragments arranged by size:
2419 1371 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 1314 1562 1314
Size: 248 3636
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 1873 2000 1873
Size: 127 3757
MspA1I CmG'CkG
Cuts at: 809 995 1309 1669 2682 2927 809
Size: 186 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245 186
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 1315 1563 1315
Size: 248 3636
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 619 937 1437 2344 619
Size: 318 500 907 2159
Fragments arranged by size:
2159 907 500 318
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PflMI CCAn_nnn'nTGG
Cuts at: 699 699
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PshAI GACnn'nnGTC
Cuts at: 598 598
Size: 3884
PsiI TTA'TAA
Cuts at: 380 760 380
Size: 380 3504
PstI C_TGCA'G
Cuts at: 931 1616 931
Size: 685 3199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 809 1309 1669 809
Size: 500 360 3024
Fragments arranged by size:
3024 500 360
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 927 1612 2605 2796 33
Size: 894 685 993 191 1121
Fragments arranged by size:
1121 993 894 685 191
SfoI GGC'GCC
Cuts at: 1316 1564 1316
Size: 248 3636
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 527 1018 1372 527
Size: 491 354 3039
Fragments arranged by size:
3039 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1600 2059 2268 1467
Size: 133 459 209 3083
Fragments arranged by size:
3083 459 209 133
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 3852
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1379 1379
Size: 3884
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI