Restriction map for
pF1KE0429
(Circular) (Six-base) MAPSORT of: pF1KE0429.seq Check: 1276 from: 1 to: 4397
pF1KE0429 4397 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1323 1323
Size: 4397
AccI GT'mk_AC
Cuts at: 1435 1435
Size: 4397
Acc65I G'GTAC_C
Cuts at: 773 1413 773
Size: 640 3757
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 2222 2654 3401 3669 587
Size: 1635 432 747 268 1315
Fragments arranged by size:
1635 1315 747 432 268
AflIII A'CryG_T
Cuts at: 1749 2853 3830 1749
Size: 1104 977 2316
Fragments arranged by size:
2316 1104 977
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1342 1374 1342
Size: 32 4365
AlwNI CAG_nnn'CTG
Cuts at: 1702 3269 1702
Size: 1567 2830
ApaI G_GGCC'C
Cuts at: 127 210 371 127
Size: 83 161 4153
Fragments arranged by size:
4153 161 83
ApaLI G'TGCA_C
Cuts at: 836 3167 836
Size: 2331 2066
ApoI r'AATT_y
Cuts at: 1401 3750 4006 1401
Size: 2349 256 1792
Fragments arranged by size:
2349 1792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 292 364 370 1417 292
Size: 72 6 1047 3272
Fragments arranged by size:
3272 1047 72 6
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 773 1335 1413 1827 2075 2110 773
Size: 562 78 414 248 35 3060
Fragments arranged by size:
3060 562 414 248 78 35
BanII G_rGCy'C
Cuts at: 127 210 371 451 1411 2441 127
Size: 83 161 80 960 1030 2083
Fragments arranged by size:
2083 1030 960 161 83 80
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BbsI GAAGACnn'nnnn_
Cuts at: 797 797
Size: 4397
BbvCI CC'TCA_GC
Cuts at: 1316 1316
Size: 4397
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 276 1917 276
Size: 1641 2756
BglI GCCn_nnn'nGGC
Cuts at: 729 729
Size: 4397
BglII A'GATC_T
Cuts at: 1912 3949 1912
Size: 2037 2360
BlpI GC'TnA_GC
Cuts at: 1509 1509
Size: 4397
Bme1580I G_kGCm'C
Cuts at: 127 210 365 371 840 1340 1387 2022 2115
Size: 83 155 6 469 500 47 635 93
Cuts at: 2115 3171 127
Size: 1056 1353
Fragments arranged by size:
1353 1056 635 500 469 155 93 83
47 6
BmrI ACTGGGnnnn_n'
Cuts at: 609 692 944 1609 1690 2025 4169 609
Size: 83 252 665 81 335 2144 837
Fragments arranged by size:
2144 837 665 335 252 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 4397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1303 459
Size: 844 3553
Bpu10I CC'TnA_GC
Cuts at: 236 1011 1316 236
Size: 775 305 3317
Fragments arranged by size:
3317 775 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1573 2452 2944 3242 196
Size: 1377 879 492 298 1351
Fragments arranged by size:
1377 1351 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 1750 1750
Size: 4397
BsaBI GATnn'nnATC
Cuts at: 6 1911 3948 6
Size: 1905 2037 455
Fragments arranged by size:
2037 1905 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1586 2107 2785 3059 3206 3617 1586
Size: 521 678 274 147 411 2366
Fragments arranged by size:
2366 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1203 1233 1203
Size: 30 4367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 534 663 1077 1080 534
Size: 129 414 3 3851
Fragments arranged by size:
3851 414 129 3
BseYI C'CCAG_C
Cuts at: 768 1167 1822 2580 3157 3653 768
Size: 399 655 758 577 496 1512
Fragments arranged by size:
1512 758 655 577 496 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 438
Size: 4397
BsiEI CG_ry'CG
Cuts at: 104 1939 1985 3193 104
Size: 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 451 840 1411 2189 2379 3171 451
Size: 389 571 778 190 792 1677
Fragments arranged by size:
1677 792 778 571 389 190
Bsp1286I G_dGCh'C
Cuts at: 127 210 365 371 451 840 1340 1387 1411
Size: 83 155 6 80 389 500 47 24
Cuts at: 1411 2022 2115 2189 2379 2441 3171 127
Size: 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 500 389 190 155 93
83 80 74 62 47 24 6
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 3573 3573
Size: 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 834 1323 1447 1661 1963 2344 834
Size: 489 124 214 302 381 2887
Fragments arranged by size:
2887 489 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 221 1250 2689 2743 4227 4392 50
Size: 171 1029 1439 54 1484 165 55
Fragments arranged by size:
1484 1439 1029 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2309 2309
Size: 4397
BsrFI r'CCGG_y
Cuts at: 2395 2576 2785 2395
Size: 181 209 4007
Fragments arranged by size:
4007 209 181
BssSI C'ACGA_G
Cuts at: 1380 2668 3026 1380
Size: 1288 358 2751
Fragments arranged by size:
2751 1288 358
BstAPI GCAn_nnn'nTGC
Cuts at: 316 1021 1335 1774 316
Size: 705 314 439 2939
Fragments arranged by size:
2939 705 439 314
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstEII G'GTnAC_C
Cuts at: 297 1140 297
Size: 843 3554
BstYI r'GATC_y
Cuts at: 1 1095 1357 1422 1889 1912 2247 2493 3505
Size: 1094 262 65 467 23 335 246 1012
Cuts at: 3505 3591 3603 3949 1
Size: 86 12 346 449
Fragments arranged by size:
1094 1012 467 449 346 335 262 246
86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 590 661 590
Size: 71 4326
BtgI C'CryG_G
Cuts at: 107 491 107
Size: 384 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2347 2528 2691 2347
Size: 181 163 4053
Fragments arranged by size:
4053 181 163
BtsI GCAGTG_nn'
Cuts at: 540 838 1640 540
Size: 298 802 3297
Fragments arranged by size:
3297 802 298
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DraIII CAC_nnn'GTG
Cuts at: 617 617
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 256 978 2103 2961 256
Size: 722 1125 858 1692
Fragments arranged by size:
1692 1125 858 722
EaeI y'GGCC_r
Cuts at: 1982 2156 2547 2574 3919 1982
Size: 174 391 27 1345 2460
Fragments arranged by size:
2460 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 606 1119 2420 2630 606
Size: 513 1301 210 2373
Fragments arranged by size:
2373 1301 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2605 2915 3061 4207 2605
Size: 310 146 1146 2795
Fragments arranged by size:
2795 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 1409 449
Size: 960 3437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 587 1303 2222 2654 3401 3669 459
Size: 128 716 919 432 747 268 1187
Fragments arranged by size:
1187 919 747 716 432 268 128
EcoO109I rG'GnC_Cy
Cuts at: 123 206 207 249 288 1029 1075 1296 1536
Size: 83 1 42 39 741 46 221 240
Cuts at: 1536 123
Size: 2984
Fragments arranged by size:
2984 741 240 221 83 46 42 39
1
EcoRI G'AATT_C
Cuts at: 1401 1401
Size: 4397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4397
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 1831 2079 3101 4080 1831
Size: 248 1022 979 2148
Fragments arranged by size:
2148 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2239 2269 2271 2301 2239
Size: 30 2 30 4335
Fragments arranged by size:
4335 30 30 2
HincII GTy'rAC
Cuts at: 1436 1436
Size: 4397
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 777 1417 777
Size: 640 3757
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3252 3068
Size: 184 4213
MscI TGG'CCA
Cuts at: 2158 2158
Size: 4397
MslI CAynn'nnrTG
Cuts at: 2386 2513 2386
Size: 127 4270
MspA1I CmG'CkG
Cuts at: 549 1171 1228 1326 1508 1822 2182 3195 3440
Size: 622 57 98 182 314 360 1013 245
Cuts at: 3440 549
Size: 1506
Fragments arranged by size:
1506 1013 622 360 314 245 182 98
57
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 4397
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 107
Size: 4397
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 4397
NspI r_CATG'y
Cuts at: 1450 1950 2857 1450
Size: 500 907 2990
Fragments arranged by size:
2990 907 500
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PfoI T'CCnGG_A
Cuts at: 439 1369 439
Size: 930 3467
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PpuMI rG'GwC_Cy
Cuts at: 249 288 1029 249
Size: 39 741 3617
Fragments arranged by size:
3617 741 39
PshAI GACnn'nnGTC
Cuts at: 182 182
Size: 4397
PspOMI G'GGCC_C
Cuts at: 123 206 367 123
Size: 83 161 4153
Fragments arranged by size:
4153 161 83
PstI C_TGCA'G
Cuts at: 383 845 874 1026 1227 1331 1444 2129 383
Size: 462 29 152 201 104 113 685 2651
Fragments arranged by size:
2651 685 462 201 152 113 104 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 549 1171 1228 1326 1822 2182 549
Size: 622 57 98 496 360 2764
Fragments arranged by size:
2764 622 496 360 98 57
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 451 1411 451
Size: 960 3437
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SanDI GG'GwC_CC
Cuts at: 288 1029 288
Size: 741 3656
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 3914 3914
Size: 4397
SfcI C'TryA_G
Cuts at: 33 379 483 841 870 1022 1037 1223 1327
Size: 346 104 358 29 152 15 186 104
Cuts at: 1327 1440 2125 3118 3309 33
Size: 113 685 993 191 1121
Fragments arranged by size:
1121 993 685 358 346 191 186 152
113 104 104 29 15
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 366 372 1419 366
Size: 6 1047 3344
Fragments arranged by size:
3344 1047 6
SmlI C'TyrA_G
Cuts at: 211 1552 2467 2959 3221 211
Size: 1341 915 492 262 1387
Fragments arranged by size:
1387 1341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 1450 1950 1450
Size: 500 3897
SrfI GCCC'GGGC
Cuts at: 366 372 366
Size: 6 4391
StuI AGG'CCT
Cuts at: 347 588 688 347
Size: 241 100 4056
Fragments arranged by size:
4056 241 100
StyI C'CwwG_G
Cuts at: 107 1531 1885 107
Size: 1424 354 2619
Fragments arranged by size:
2619 1424 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 628 963 1980 2113 2572 2781 628
Size: 335 1017 133 459 209 2244
Fragments arranged by size:
2244 1017 459 335 209 133
TatI w'GTAC_w
Cuts at: 700 3912 700
Size: 3212 1185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 883 915 1203 1235 3914 3946 103
Size: 32 748 32 288 32 2679 32 554
Fragments arranged by size:
2679 748 554 288 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2194 2194
Size: 4397
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 268 1892 268
Size: 1624 2773
XmaI C'CCGG_G
Cuts at: 364 370 1417 364
Size: 6 1047 3344
Fragments arranged by size:
3344 1047 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI