Restriction map for
pF1KE0427
(Circular) (Six-base) MAPSORT of: pF1KE0427.seq Check: 8036 from: 1 to: 3647
pF1KE0427 3647 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 685 685
Size: 3647
Acc65I G'GTAC_C
Cuts at: 663 663
Size: 3647
AclI AA'CG_TT
Cuts at: 3487 3487
Size: 3647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1472 1904 2651 2919 650
Size: 822 432 747 268 1378
Fragments arranged by size:
1378 822 747 432 268
AflIII A'CryG_T
Cuts at: 470 999 2103 3080 470
Size: 529 1104 977 1037
Fragments arranged by size:
1104 1037 977 529
AgeI A'CCGG_T
Cuts at: 2035 2035
Size: 3647
AlwNI CAG_nnn'CTG
Cuts at: 952 2519 952
Size: 1567 2080
ApaLI G'TGCA_C
Cuts at: 2417 2417
Size: 3647
ApoI r'AATT_y
Cuts at: 352 651 3000 3256 352
Size: 299 2349 256 743
Fragments arranged by size:
2349 743 299 256
AseI AT'TA_AT
Cuts at: 20 265 20
Size: 245 3402
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
AvaI C'yCGr_G
Cuts at: 667 667
Size: 3647
BamHI G'GATC_C
Cuts at: 168 672 168
Size: 504 3143
BanI G'GyrC_C
Cuts at: 249 663 1077 1325 1360 249
Size: 414 414 248 35 2536
Fragments arranged by size:
2536 414 414 248 35
BanII G_rGCy'C
Cuts at: 661 1691 661
Size: 1030 2617
BbeI G_GCGC'C
Cuts at: 1081 1329 1081
Size: 248 3399
BbsI GAAGACnn'nnnn_
Cuts at: 638 638
Size: 3647
BciVI GTATCCnnnnn_n'
Cuts at: 576 1545 2306 3647 576
Size: 969 761 1341 576
Fragments arranged by size:
1341 969 761 576
BclI T'GATC_A
Cuts at: 1167 1167
Size: 3647
BglII A'GATC_T
Cuts at: 1162 3199 1162
Size: 2037 1610
BlpI GC'TnA_GC
Cuts at: 759 759
Size: 3647
Bme1580I G_kGCm'C
Cuts at: 326 1272 1365 2421 326
Size: 946 93 1056 1552
Fragments arranged by size:
1552 1056 946 93
BmgBI CAC'GTC
Cuts at: 327 327
Size: 3647
BmrI ACTGGGnnnn_n'
Cuts at: 859 940 1275 3419 859
Size: 81 335 2144 1087
Fragments arranged by size:
2144 1087 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1702 2194 2492 823
Size: 879 492 298 1978
Fragments arranged by size:
1978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3351 3351
Size: 3647
BsaAI yAC'GTr
Cuts at: 1000 1000
Size: 3647
BsaBI GATnn'nnATC
Cuts at: 6 1161 3198 6
Size: 1155 2037 455
Fragments arranged by size:
2037 1155 455
BsaHI Gr'CG_yC
Cuts at: 360 1078 1326 2028 3524 360
Size: 718 248 702 1496 483
Fragments arranged by size:
1496 718 702 483 248
BsaWI w'CCGG_w
Cuts at: 836 1357 2035 2309 2456 2867 836
Size: 521 678 274 147 411 1616
Fragments arranged by size:
1616 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 412
Size: 3647
BseYI C'CCAG_C
Cuts at: 1072 1830 2407 2903 1072
Size: 758 577 496 1816
Fragments arranged by size:
1816 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1189 1235 2443 104
Size: 1085 46 1208 1308
Fragments arranged by size:
1308 1208 1085 46
BsiHKAI G_wGCw'C
Cuts at: 661 1439 1629 2421 661
Size: 778 190 792 1887
Fragments arranged by size:
1887 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 326 661 1272 1365 1439 1629 1691 2421 326
Size: 335 611 93 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 335 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2867 2867
Size: 3647
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 139 697 911 1213 1594 139
Size: 558 214 302 381 2192
Fragments arranged by size:
2192 558 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1939 1993 3477 3642 50
Size: 1889 54 1484 165 55
Fragments arranged by size:
1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1559 1559
Size: 3647
BsrFI r'CCGG_y
Cuts at: 1645 1826 2035 1645
Size: 181 209 3257
Fragments arranged by size:
3257 209 181
BsrGI T'GTAC_A
Cuts at: 473 473
Size: 3647
BssSI C'ACGA_G
Cuts at: 1918 2276 1918
Size: 358 3289
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1024
Size: 3647
BstBI TT'CG_AA
Cuts at: 2008 2008
Size: 3647
BstYI r'GATC_y
Cuts at: 1 168 432 672 1139 1162 1497 1743 2755
Size: 167 264 240 467 23 335 246 1012
Cuts at: 2755 2841 2853 3199 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 264 246 240
167 86 23 12
BtgI C'CryG_G
Cuts at: 118 118
Size: 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1597 1778 1941 1597
Size: 181 163 3303
Fragments arranged by size:
3303 181 163
BtsI GCAGTG_nn'
Cuts at: 251 890 251
Size: 639 3008
DraI TTT'AAA
Cuts at: 646 646
Size: 3647
DraIII CAC_nnn'GTG
Cuts at: 256 256
Size: 3647
DrdI GACnn_nn'nnGTC
Cuts at: 1353 2211 1353
Size: 858 2789
EaeI y'GGCC_r
Cuts at: 223 524 1232 1406 1797 1824 3169 223
Size: 301 708 174 391 27 1345 701
Fragments arranged by size:
1345 708 701 391 301 174 27
EagI C'GGCC_G
Cuts at: 1232 1232
Size: 3647
EarI CTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1855 2165 2311 3457 1855
Size: 310 146 1146 2045
Fragments arranged by size:
2045 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 659
Size: 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1472 1904 2651 2919 650
Size: 822 432 747 268 1378
Fragments arranged by size:
1378 822 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 786 786
Size: 3647
EcoRI G'AATT_C
Cuts at: 651 651
Size: 3647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1666 1666
Size: 3647
FspI TGC'GCA
Cuts at: 1428 1428
Size: 3647
HaeII r_GCGC'y
Cuts at: 1081 1329 2351 3330 1081
Size: 248 1022 979 1398
Fragments arranged by size:
1398 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 1489 1519 1521 1551 580
Size: 32 877 30 2 30 2676
Fragments arranged by size:
2676 877 32 30 30 2
HincII GTy'rAC
Cuts at: 468 686 468
Size: 218 3429
HpaI GTT'AAC
Cuts at: 468 468
Size: 3647
KasI G'GCGC_C
Cuts at: 1077 1325 1077
Size: 248 3399
KpnI G_GTAC'C
Cuts at: 667 667
Size: 3647
MluI A'CGCG_T
Cuts at: 3080 3080
Size: 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2502 2318
Size: 184 3463
MscI TGG'CCA
Cuts at: 225 1408 225
Size: 1183 2464
MslI CAynn'nnrTG
Cuts at: 1636 1763 1636
Size: 127 3520
MspA1I CmG'CkG
Cuts at: 758 1072 1432 2445 2690 758
Size: 314 360 1013 245 1715
Fragments arranged by size:
1715 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1828 1828
Size: 3647
NarI GG'CG_CC
Cuts at: 1078 1326 1078
Size: 248 3399
NgoMIV G'CCGG_C
Cuts at: 1826 1826
Size: 3647
NspI r_CATG'y
Cuts at: 474 700 1200 2107 474
Size: 226 500 907 2014
Fragments arranged by size:
2014 907 500 226
PciI A'CATG_T
Cuts at: 470 2103 470
Size: 1633 2014
PmeI GTTT'AAAC
Cuts at: 646 646
Size: 3647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3615
PstI C_TGCA'G
Cuts at: 694 1379 694
Size: 685 2962
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3647
PvuII CAG'CTG
Cuts at: 1072 1432 1072
Size: 360 3287
RsrII CG'GwC_CG
Cuts at: 1842 1842
Size: 3647
SacI G_AGCT'C
Cuts at: 661 661
Size: 3647
SalI G'TCGA_C
Cuts at: 684 684
Size: 3647
SapI GCTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
SbfI CC_TGCA'GG
Cuts at: 694 694
Size: 3647
ScaI AGT'ACT
Cuts at: 517 3164 517
Size: 2647 1000
SexAI A'CCwGG_T
Cuts at: 252 252
Size: 3647
SfcI C'TryA_G
Cuts at: 33 489 690 1375 2368 2559 33
Size: 456 201 685 993 191 1121
Fragments arranged by size:
1121 993 685 456 201 191
SfoI GGC'GCC
Cuts at: 1079 1327 1079
Size: 248 3399
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
SmaI CCC'GGG
Cuts at: 669 669
Size: 3647
SmlI C'TyrA_G
Cuts at: 802 1717 2209 2471 802
Size: 915 492 262 1978
Fragments arranged by size:
1978 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3647
SphI G_CATG'C
Cuts at: 700 1200 700
Size: 500 3147
StuI AGG'CCT
Cuts at: 627 627
Size: 3647
StyI C'CwwG_G
Cuts at: 363 781 1135 363
Size: 418 354 2875
Fragments arranged by size:
2875 418 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1230 1363 1822 2031 1230
Size: 133 459 209 2846
Fragments arranged by size:
2846 459 209 133
TatI w'GTAC_w
Cuts at: 473 515 3162 473
Size: 42 2647 958
Fragments arranged by size:
2647 958 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3615
Tth111I GACn'n_nGTC
Cuts at: 1444 1444
Size: 3647
XbaI T'CTAG_A
Cuts at: 678 678
Size: 3647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1142 1142
Size: 3647
XmaI C'CCGG_G
Cuts at: 667 667
Size: 3647
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI