Restriction map for
pF1KE0420
(Circular) (Six-base) MAPSORT of: pF1KE0420.seq Check: 2725 from: 1 to: 3731
pF1KE0420 3731 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 582 582
Size: 3731
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 185 747 185
Size: 562 3169
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1988 2735 3003 1556
Size: 432 747 268 2284
Fragments arranged by size:
2284 747 432 268
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 275 302 738 275
Size: 27 436 3268
Fragments arranged by size:
3268 436 27
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 735 3084 3340 735
Size: 2349 256 1126
Fragments arranged by size:
2349 1126 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 751 751
Size: 3731
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 282 315 282
Size: 33 3698
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 185 343 747 1161 1409 1444 185
Size: 158 404 414 248 35 2472
Fragments arranged by size:
2472 414 404 248 158 35
BanII G_rGCy'C
Cuts at: 443 722 745 1775 443
Size: 279 23 1030 2399
Fragments arranged by size:
2399 1030 279 23
BbeI G_GCGC'C
Cuts at: 347 1165 1413 347
Size: 818 248 2665
Fragments arranged by size:
2665 818 248
BbsI GAAGACnn'nnnn_
Cuts at: 407 407
Size: 3731
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglII A'GATC_T
Cuts at: 393 1246 3283 393
Size: 853 2037 841
Fragments arranged by size:
2037 853 841
BlpI GC'TnA_GC
Cuts at: 843 843
Size: 3731
Bme1580I G_kGCm'C
Cuts at: 182 1356 1449 2505 182
Size: 1174 93 1056 1408
Fragments arranged by size:
1408 1174 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 688 268
Size: 420 3311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1786 2278 2576 907
Size: 879 492 298 2062
Fragments arranged by size:
2062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 684 1084 684
Size: 400 3331
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 344 1162 1410 2112 3608 344
Size: 818 248 702 1496 467
Fragments arranged by size:
1496 818 702 467 248
BsaWI w'CCGG_w
Cuts at: 563 920 1441 2119 2393 2540 2951 563
Size: 357 521 678 274 147 411 1343
Fragments arranged by size:
1343 678 521 411 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 241
Size: 30 3701
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 223
Size: 3731
BseYI C'CCAG_C
Cuts at: 135 335 442 1156 1914 2491 2987 135
Size: 200 107 714 758 577 496 879
Fragments arranged by size:
879 758 714 577 496 200 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 291
Size: 3731
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 745 1523 1713 2505 745
Size: 778 190 792 1971
Fragments arranged by size:
1971 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 182 443 722 745 1356 1449 1523 1713 1775
Size: 261 279 23 611 93 74 190 62
Cuts at: 1775 2505 182
Size: 730 1408
Fragments arranged by size:
1408 730 611 279 261 190 93 74
62 23
BspEI T'CCGG_A
Cuts at: 563 2951 563
Size: 2388 1343
BspHI T'CATG_A
Cuts at: 428 2907 428
Size: 2479 1252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 781 995 1297 1678 582
Size: 199 214 302 381 2635
Fragments arranged by size:
2635 381 302 214 199
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BssSI C'ACGA_G
Cuts at: 190 2002 2360 190
Size: 1812 358 1561
Fragments arranged by size:
1812 1561 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstEII G'GTnAC_C
Cuts at: 621 621
Size: 3731
BstXI CCAn_nnnn'nTGG
Cuts at: 684 684
Size: 3731
BstYI r'GATC_y
Cuts at: 1 393 756 1223 1246 1581 1827 2839 2925
Size: 392 363 467 23 335 246 1012 86
Cuts at: 2925 2937 3283 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 392 363 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 499 1681 1862 2025 499
Size: 1182 181 163 2205
Fragments arranged by size:
2205 1182 181 163
BtsI GCAGTG_nn'
Cuts at: 274 612 974 274
Size: 338 362 3031
Fragments arranged by size:
3031 362 338
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 1316 1490 1881 1908 3253 1316
Size: 174 391 27 1345 1794
Fragments arranged by size:
1794 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 347 388 683 1754 1964 347
Size: 41 295 1071 210 2114
Fragments arranged by size:
2114 1071 295 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 688 1556 1988 2735 3003 268
Size: 420 868 432 747 268 996
Fragments arranged by size:
996 868 747 432 420 268
EcoNI CCTnn'n_nnAGG
Cuts at: 250 250
Size: 3731
EcoO109I rG'GnC_Cy
Cuts at: 459 870 459
Size: 411 3320
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 318 1750 318
Size: 1432 2299
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 347 1165 1413 2435 3414 347
Size: 818 248 1022 979 664
Fragments arranged by size:
1022 979 818 664 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 545 577 1573 1603 1605 1635 102
Size: 32 411 32 996 30 2 30 2198
Fragments arranged by size:
2198 996 411 32 32 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 343 1161 1409 343
Size: 818 248 2665
Fragments arranged by size:
2665 818 248
KpnI G_GTAC'C
Cuts at: 189 751 189
Size: 562 3169
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 565 1720 1847 565
Size: 1155 127 2449
Fragments arranged by size:
2449 1155 127
MspA1I CmG'CkG
Cuts at: 240 275 446 842 1156 1516 2529 2774 240
Size: 35 171 396 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 396 360 314 245 171 35
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 344 1162 1410 344
Size: 818 248 2665
Fragments arranged by size:
2665 818 248
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 178 784 1284 2191 178
Size: 606 500 907 1718
Fragments arranged by size:
1718 907 606 500
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PmlI CAC'GTG
Cuts at: 684 684
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PstI C_TGCA'G
Cuts at: 218 451 778 1463 218
Size: 233 327 685 2486
Fragments arranged by size:
2486 685 327 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 275 446 1156 1516 275
Size: 171 710 360 2490
Fragments arranged by size:
2490 710 360 171
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 214 447 774 1459 2452 2643 33
Size: 181 233 327 685 993 191 1121
Fragments arranged by size:
1121 993 685 327 233 191 181
SfoI GGC'GCC
Cuts at: 345 1163 1411 345
Size: 818 248 2665
Fragments arranged by size:
2665 818 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 886 1801 2293 2555 886
Size: 915 492 262 2062
Fragments arranged by size:
2062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 410 462 865 1219 410
Size: 52 403 354 2922
Fragments arranged by size:
2922 403 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 3246 3246
Size: 3731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 396 762 396
Size: 366 3365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
XmnI GAAnn'nnTTC
Cuts at: 382 382
Size: 3731
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI