Restriction map for
pF1KE0417
(Circular) (Six-base) MAPSORT of: pF1KE0417.seq Check: 6415 from: 1 to: 3875
pF1KE0417 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 463 1700 2132 2879 3147 242
Size: 221 1237 432 747 268 970
Fragments arranged by size:
1237 970 747 432 268 221
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AhdI GACnn_n'nnGTC
Cuts at: 780 780
Size: 3875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 532 564 532
Size: 32 3843
AlwNI CAG_nnn'CTG
Cuts at: 574 1180 2747 574
Size: 606 1567 1702
Fragments arranged by size:
1702 1567 606
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 328 476 879 3228 3484 328
Size: 148 403 2349 256 719
Fragments arranged by size:
2349 719 403 256 148
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 859 895 859
Size: 36 3839
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 754 802 891 1305 1553 1588 754
Size: 48 89 414 248 35 3041
Fragments arranged by size:
3041 414 248 89 48 35
BanII G_rGCy'C
Cuts at: 592 659 889 1919 592
Size: 67 230 1030 2548
Fragments arranged by size:
2548 1030 230 67
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 657 691 657
Size: 34 3841
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 804 2242 804
Size: 1438 2437
Bpu10I CC'TnA_GC
Cuts at: 632 632
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 467 1051 1930 2422 2720 448
Size: 19 584 879 492 298 1603
Fragments arranged by size:
1603 879 584 492 298 19
BsaI GGTCTCn'nnnn_
Cuts at: 358 3579 358
Size: 3221 654
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 624 817 1064 1585 2263 2537 2684 3095 624
Size: 193 247 521 678 274 147 411 1404
Fragments arranged by size:
1404 678 521 411 274 247 193 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 532 562 532
Size: 30 3845
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 822 259
Size: 563 3312
BseYI C'CCAG_C
Cuts at: 1300 2058 2635 3131 1300
Size: 758 577 496 2044
Fragments arranged by size:
2044 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 592 889 1667 1857 2649 592
Size: 297 778 190 792 1818
Fragments arranged by size:
1818 792 778 297 190
Bsp1286I G_dGCh'C
Cuts at: 592 659 889 1500 1593 1667 1857 1919 2649
Size: 67 230 611 93 74 190 62 730
Cuts at: 2649 592
Size: 1818
Fragments arranged by size:
1818 730 611 230 190 93 74 67
62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 1787
Size: 3875
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 213 2236 213
Size: 2023 1852
BstEII G'GTnAC_C
Cuts at: 127 127
Size: 3875
BstYI r'GATC_y
Cuts at: 1 225 900 1367 1390 1725 1971 2983 3069
Size: 224 675 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 675 467 449 346 335 246 224
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 708 1118 708
Size: 410 3465
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 1460 1634 2025 2052 3397 1460
Size: 174 391 27 1345 1938
Fragments arranged by size:
1938 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 414 1898 2108 414
Size: 1484 210 2181
Fragments arranged by size:
2181 1484 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 590 887 590
Size: 297 3578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 463 1700 2132 2879 3147 242
Size: 221 1237 432 747 268 970
Fragments arranged by size:
1237 970 747 432 268 221
EcoNI CCTnn'n_nnAGG
Cuts at: 379 466 379
Size: 87 3788
EcoO109I rG'GnC_Cy
Cuts at: 864 1014 864
Size: 150 3725
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 646 678 766 798 1717 1747 1749
Size: 32 367 32 88 32 919 30 2
Cuts at: 1749 1779 247
Size: 30 2343
Fragments arranged by size:
2343 919 367 88 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 453 2546 2730 453
Size: 2093 184 1598
Fragments arranged by size:
2093 1598 184
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 986 1300 1660 2673 2918 986
Size: 314 360 1013 245 1943
Fragments arranged by size:
1943 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 107
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 928 1428 2335 928
Size: 500 907 2468
Fragments arranged by size:
2468 907 500
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 307 339 532 564 3014 3046 307
Size: 32 193 32 2450 32 1136
Fragments arranged by size:
2450 1136 193 32 32 32
PspXI vC'TCGA_Gb
Cuts at: 859 859
Size: 3875
PstI C_TGCA'G
Cuts at: 726 922 1607 726
Size: 196 685 2994
Fragments arranged by size:
2994 685 196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 1300 1660 1300
Size: 360 3515
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 592 889 592
Size: 297 3578
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 280 527 722 918 1603 2596 2787 33
Size: 247 247 195 196 685 993 191 1121
Fragments arranged by size:
1121 993 685 247 247 196 195 191
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 463 482 859 1030 1945 2437 2699 463
Size: 19 377 171 915 492 262 1639
Fragments arranged by size:
1639 915 492 377 262 171 19
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StuI AGG'CCT
Cuts at: 749 749
Size: 3875
StyI C'CwwG_G
Cuts at: 107 1009 1363 107
Size: 902 354 2619
Fragments arranged by size:
2619 902 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 742 1458 1591 2050 2259 742
Size: 716 133 459 209 2358
Fragments arranged by size:
2358 716 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XhoI C'TCGA_G
Cuts at: 859 859
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
XmnI GAAnn'nnTTC
Cuts at: 544 544
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI