Restriction map for
pF1KE0415
(Circular) (Six-base) MAPSORT of: pF1KE0415.seq Check: 3083 from: 1 to: 3584
pF1KE0415 3584 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 488 622 488
Size: 134 3450
Acc65I G'GTAC_C
Cuts at: 600 600
Size: 3584
AclI AA'CG_TT
Cuts at: 3424 3424
Size: 3584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1409 1841 2588 2856 584
Size: 825 432 747 268 1312
Fragments arranged by size:
1312 825 747 432 268
AflIII A'CryG_T
Cuts at: 936 2040 3017 936
Size: 1104 977 1503
Fragments arranged by size:
1503 1104 977
AgeI A'CCGG_T
Cuts at: 1972 1972
Size: 3584
AlwNI CAG_nnn'CTG
Cuts at: 889 2456 889
Size: 1567 2017
ApaLI G'TGCA_C
Cuts at: 2354 2354
Size: 3584
ApoI r'AATT_y
Cuts at: 588 2937 3193 588
Size: 2349 256 979
Fragments arranged by size:
2349 979 256
AseI AT'TA_AT
Cuts at: 20 317 20
Size: 297 3287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3584
AvaI C'yCGr_G
Cuts at: 604 604
Size: 3584
BamHI G'GATC_C
Cuts at: 609 609
Size: 3584
BanI G'GyrC_C
Cuts at: 600 1014 1262 1297 600
Size: 414 248 35 2887
Fragments arranged by size:
2887 414 248 35
BanII G_rGCy'C
Cuts at: 598 1628 598
Size: 1030 2554
BbeI G_GCGC'C
Cuts at: 1018 1266 1018
Size: 248 3336
BbsI GAAGACnn'nnnn_
Cuts at: 572 572
Size: 3584
BciVI GTATCCnnnnn_n'
Cuts at: 1482 2243 3584 1482
Size: 761 1341 1482
Fragments arranged by size:
1482 1341 761
BclI T'GATC_A
Cuts at: 1104 1104
Size: 3584
BfrBI ATG'CAT
Cuts at: 146 146
Size: 3584
BglII A'GATC_T
Cuts at: 1099 3136 1099
Size: 2037 1547
BlpI GC'TnA_GC
Cuts at: 696 696
Size: 3584
Bme1580I G_kGCm'C
Cuts at: 1209 1302 2358 1209
Size: 93 1056 2435
Fragments arranged by size:
2435 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 162 796 877 1212 3356 162
Size: 634 81 335 2144 390
Fragments arranged by size:
2144 634 390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1951 1951
Size: 3584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 760 1639 2131 2429 150
Size: 610 879 492 298 1305
Fragments arranged by size:
1305 879 610 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3288 3288
Size: 3584
BsaAI yAC'GTr
Cuts at: 937 937
Size: 3584
BsaBI GATnn'nnATC
Cuts at: 6 1098 3135 6
Size: 1092 2037 455
Fragments arranged by size:
2037 1092 455
BsaHI Gr'CG_yC
Cuts at: 1015 1263 1965 3461 1015
Size: 248 702 1496 1138
Fragments arranged by size:
1496 1138 702 248
BsaWI w'CCGG_w
Cuts at: 773 1294 1972 2246 2393 2804 773
Size: 521 678 274 147 411 1553
Fragments arranged by size:
1553 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 449 479 449
Size: 30 3554
BseYI C'CCAG_C
Cuts at: 1009 1767 2344 2840 1009
Size: 758 577 496 1753
Fragments arranged by size:
1753 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1126 1172 2380 104
Size: 1022 46 1208 1308
Fragments arranged by size:
1308 1208 1022 46
BsiHKAI G_wGCw'C
Cuts at: 387 598 1376 1566 2358 387
Size: 211 778 190 792 1613
Fragments arranged by size:
1613 792 778 211 190
BsmI GAATG_Cn'
Cuts at: 148 522 148
Size: 374 3210
Bsp1286I G_dGCh'C
Cuts at: 387 598 1209 1302 1376 1566 1628 2358 387
Size: 211 611 93 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 211 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2804 2804
Size: 3584
BspHI T'CATG_A
Cuts at: 2760 2760
Size: 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 567 634 848 1150 1531 567
Size: 67 214 302 381 2620
Fragments arranged by size:
2620 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1876 1930 3414 3579 50
Size: 1826 54 1484 165 55
Fragments arranged by size:
1826 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 1496 182
Size: 1314 2270
BsrFI r'CCGG_y
Cuts at: 1582 1763 1972 1582
Size: 181 209 3194
Fragments arranged by size:
3194 209 181
BssSI C'ACGA_G
Cuts at: 1855 2213 1855
Size: 358 3226
BstAPI GCAn_nnn'nTGC
Cuts at: 961 961
Size: 3584
BstBI TT'CG_AA
Cuts at: 1945 1945
Size: 3584
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 3584
BstYI r'GATC_y
Cuts at: 1 424 609 1076 1099 1434 1680 2692 2778
Size: 423 185 467 23 335 246 1012 86
Cuts at: 2778 2790 3136 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 246 185
86 23 12
BstZ17I GTA'TAC
Cuts at: 489 489
Size: 3584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1534 1715 1878 1534
Size: 181 163 3240
Fragments arranged by size:
3240 181 163
BtsI GCAGTG_nn'
Cuts at: 827 827
Size: 3584
DraI TTT'AAA
Cuts at: 583 583
Size: 3584
DrdI GACnn_nn'nnGTC
Cuts at: 1290 2148 1290
Size: 858 2726
EaeI y'GGCC_r
Cuts at: 555 1169 1343 1734 1761 3106 555
Size: 614 174 391 27 1345 1033
Fragments arranged by size:
1345 1033 614 391 174 27
EagI C'GGCC_G
Cuts at: 1169 1169
Size: 3584
EarI CTCTTCn'nnn_
Cuts at: 1607 1817 1607
Size: 210 3374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1792 2102 2248 3394 1792
Size: 310 146 1146 1982
Fragments arranged by size:
1982 1146 310 146
EcoICRI GAG'CTC
Cuts at: 596 596
Size: 3584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1409 1841 2588 2856 584
Size: 825 432 747 268 1312
Fragments arranged by size:
1312 825 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 723 723
Size: 3584
EcoRI G'AATT_C
Cuts at: 588 588
Size: 3584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1603 1603
Size: 3584
FspI TGC'GCA
Cuts at: 1365 1365
Size: 3584
HaeII r_GCGC'y
Cuts at: 1018 1266 2288 3267 1018
Size: 248 1022 979 1335
Fragments arranged by size:
1335 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1426 1456 1458 1488 1426
Size: 30 2 30 3522
Fragments arranged by size:
3522 30 30 2
HincII GTy'rAC
Cuts at: 623 623
Size: 3584
KasI G'GCGC_C
Cuts at: 1014 1262 1014
Size: 248 3336
KpnI G_GTAC'C
Cuts at: 604 604
Size: 3584
MluI A'CGCG_T
Cuts at: 3017 3017
Size: 3584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2439 2255
Size: 184 3400
MscI TGG'CCA
Cuts at: 1345 1345
Size: 3584
MslI CAynn'nnrTG
Cuts at: 281 441 1573 1700 281
Size: 160 1132 127 2165
Fragments arranged by size:
2165 1132 160 127
MspA1I CmG'CkG
Cuts at: 235 695 1009 1369 2382 2627 235
Size: 460 314 360 1013 245 1192
Fragments arranged by size:
1192 1013 460 360 314 245
NaeI GCC'GGC
Cuts at: 1765 1765
Size: 3584
NarI GG'CG_CC
Cuts at: 1015 1263 1015
Size: 248 3336
NgoMIV G'CCGG_C
Cuts at: 1763 1763
Size: 3584
NsiI A_TGCA'T
Cuts at: 148 148
Size: 3584
NspI r_CATG'y
Cuts at: 637 1137 2044 637
Size: 500 907 2177
Fragments arranged by size:
2177 907 500
PciI A'CATG_T
Cuts at: 2040 2040
Size: 3584
PmeI GTTT'AAAC
Cuts at: 583 583
Size: 3584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2723 2755 2723
Size: 32 3552
PstI C_TGCA'G
Cuts at: 631 1316 631
Size: 685 2899
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3584
PvuII CAG'CTG
Cuts at: 235 1009 1369 235
Size: 774 360 2450
Fragments arranged by size:
2450 774 360
RsrII CG'GwC_CG
Cuts at: 1779 1779
Size: 3584
SacI G_AGCT'C
Cuts at: 598 598
Size: 3584
SalI G'TCGA_C
Cuts at: 621 621
Size: 3584
SapI GCTCTTCn'nnn_
Cuts at: 1607 1817 1607
Size: 210 3374
SbfI CC_TGCA'GG
Cuts at: 631 631
Size: 3584
ScaI AGT'ACT
Cuts at: 3101 3101
Size: 3584
SfcI C'TryA_G
Cuts at: 33 627 1312 2305 2496 33
Size: 594 685 993 191 1121
Fragments arranged by size:
1121 993 685 594 191
SfoI GGC'GCC
Cuts at: 1016 1264 1016
Size: 248 3336
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3584
SmaI CCC'GGG
Cuts at: 606 606
Size: 3584
SmlI C'TyrA_G
Cuts at: 129 739 1654 2146 2408 129
Size: 610 915 492 262 1305
Fragments arranged by size:
1305 915 610 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3584
SphI G_CATG'C
Cuts at: 637 1137 637
Size: 500 3084
StyI C'CwwG_G
Cuts at: 718 1072 718
Size: 354 3230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1167 1300 1759 1968 1167
Size: 133 459 209 2783
Fragments arranged by size:
2783 459 209 133
TatI w'GTAC_w
Cuts at: 195 3099 195
Size: 2904 680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 3552
Tth111I GACn'n_nGTC
Cuts at: 1381 1381
Size: 3584
XbaI T'CTAG_A
Cuts at: 120 615 120
Size: 495 3089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1079 1079
Size: 3584
XmaI C'CCGG_G
Cuts at: 604 604
Size: 3584
XmnI GAAnn'nnTTC
Cuts at: 259 520 259
Size: 261 3323
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI