Restriction map for
pF1KE0413
(Circular) (Six-base) MAPSORT of: pF1KE0413.seq Check: 7472 from: 1 to: 4115
pF1KE0413 4115 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2372 3119 3387 1940
Size: 432 747 268 2668
Fragments arranged by size:
2668 747 432 268
AflII C'TTAA_G
Cuts at: 933 933
Size: 4115
AflIII A'CryG_T
Cuts at: 774 1467 2571 3548 774
Size: 693 1104 977 1341
Fragments arranged by size:
1341 1104 977 693
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AhdI GACnn_n'nnGTC
Cuts at: 1099 1099
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 430 484 700 1420 2987 430
Size: 54 216 720 1567 1558
Fragments arranged by size:
1567 1558 720 216 54
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 247 304 462 576 1119 3468 3724 247
Size: 57 158 114 543 2349 256 638
Fragments arranged by size:
2349 638 543 256 158 114 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 148 1135 148
Size: 987 3128
AvrII C'CTAG_G
Cuts at: 960 960
Size: 4115
BamHI G'GATC_C
Cuts at: 828 1140 828
Size: 312 3803
BanI G'GyrC_C
Cuts at: 211 1131 1545 1793 1828 211
Size: 920 414 248 35 2498
Fragments arranged by size:
2498 920 414 248 35
BanII G_rGCy'C
Cuts at: 131 1129 2159 131
Size: 998 1030 2087
Fragments arranged by size:
2087 1030 998
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BbsI GAAGACnn'nnnn_
Cuts at: 405 563 405
Size: 158 3957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 673 707 673
Size: 34 4081
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 986 1227 986
Size: 241 3874
Bme1580I G_kGCm'C
Cuts at: 1740 1833 2889 1740
Size: 93 1056 2966
Fragments arranged by size:
2966 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 227 1327 1408 1743 3887 227
Size: 1100 81 335 2144 455
Fragments arranged by size:
2144 1100 455 335 81
BmtI G_CTAG'C
Cuts at: 811 811
Size: 4115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 514 1001 2482 514
Size: 487 1481 2147
Fragments arranged by size:
2147 1481 487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 640
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2662 2960 1291
Size: 879 492 298 2446
Fragments arranged by size:
2446 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1003 3819 1003
Size: 2816 1299
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 1546 1794 2496 3992 1546
Size: 248 702 1496 1669
Fragments arranged by size:
1669 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 626 656 626
Size: 30 4085
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 4115
BseYI C'CCAG_C
Cuts at: 922 1540 2298 2875 3371 922
Size: 618 758 577 496 1666
Fragments arranged by size:
1666 758 618 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 713 739 407
Size: 306 26 3783
Fragments arranged by size:
3783 306 26
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 969 1129 1907 2097 2889 131
Size: 838 160 778 190 792 1357
Fragments arranged by size:
1357 838 792 778 190 160
BsmI GAATG_Cn'
Cuts at: 827 827
Size: 4115
Bsp1286I G_dGCh'C
Cuts at: 131 969 1129 1740 1833 1907 2097 2159 2889
Size: 838 160 611 93 74 190 62 730
Cuts at: 2889 131
Size: 1357
Fragments arranged by size:
1357 838 730 611 190 160 93 74
62
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 354 3291 354
Size: 2937 1178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1379 1681 2062 1165
Size: 214 302 381 3218
Fragments arranged by size:
3218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 870 2027 870
Size: 1157 2958
BsrFI r'CCGG_y
Cuts at: 800 2113 2294 2503 800
Size: 1313 181 209 2412
Fragments arranged by size:
2412 1313 209 181
BsrGI T'GTAC_A
Cuts at: 237 237
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 1041 2476 1041
Size: 1435 2680
BstYI r'GATC_y
Cuts at: 1 828 1140 1607 1630 1965 2211 3223 3309
Size: 827 312 467 23 335 246 1012 86
Cuts at: 3309 3321 3667 1
Size: 12 346 449
Fragments arranged by size:
1012 827 467 449 346 335 312 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 701 1358 701
Size: 657 3458
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DraIII CAC_nnn'GTG
Cuts at: 797 797
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 955 1821 2679 955
Size: 866 858 2391
Fragments arranged by size:
2391 866 858
EaeI y'GGCC_r
Cuts at: 788 1023 1700 1874 2265 2292 3637 788
Size: 235 677 174 391 27 1345 1266
Fragments arranged by size:
1345 1266 677 391 235 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 285 811 2138 2348 285
Size: 526 1327 210 2052
Fragments arranged by size:
2052 1327 526 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 129 1127 129
Size: 998 3117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1940 2372 3119 3387 640
Size: 1300 432 747 268 1368
Fragments arranged by size:
1368 1300 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 959 959
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 1254 1254
Size: 4115
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 864 1078 2134 864
Size: 214 1056 2845
Fragments arranged by size:
2845 1056 214
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 1549 1797 2819 3798 1549
Size: 248 1022 979 1866
Fragments arranged by size:
1866 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 1085 1117 1957 1987 1989 2019 418
Size: 32 635 32 840 30 2 30 2514
Fragments arranged by size:
2514 840 635 32 32 30 30 2
HincII GTy'rAC
Cuts at: 180 1154 180
Size: 974 3141
HpaI GTT'AAC
Cuts at: 180 180
Size: 4115
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MfeI C'AATT_G
Cuts at: 899 899
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 305 468 2786 2970 305
Size: 163 2318 184 1450
Fragments arranged by size:
2318 1450 184 163
MscI TGG'CCA
Cuts at: 790 1025 1876 790
Size: 235 851 3029
Fragments arranged by size:
3029 851 235
MslI CAynn'nnrTG
Cuts at: 2104 2231 2104
Size: 127 3988
MspA1I CmG'CkG
Cuts at: 481 1226 1540 1900 2913 3158 481
Size: 745 314 360 1013 245 1438
Fragments arranged by size:
1438 1013 745 360 314 245
NaeI GCC'GGC
Cuts at: 802 2296 802
Size: 1494 2621
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NcoI C'CATG_G
Cuts at: 107 107
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 800 2294 800
Size: 1494 2621
NheI G'CTAG_C
Cuts at: 807 807
Size: 4115
NspI r_CATG'y
Cuts at: 271 778 896 1168 1668 2575 271
Size: 507 118 272 500 907 1811
Fragments arranged by size:
1811 907 507 500 272 118
PciI A'CATG_T
Cuts at: 774 2571 774
Size: 1797 2318
PfoI T'CCnGG_A
Cuts at: 617 617
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PstI C_TGCA'G
Cuts at: 1162 1847 1162
Size: 685 3430
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 481 1540 1900 481
Size: 1059 360 2696
Fragments arranged by size:
2696 1059 360
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 131 1129 131
Size: 998 3117
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 285 2138 2348 285
Size: 1853 210 2052
Fragments arranged by size:
2052 1853 210
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SfcI C'TryA_G
Cuts at: 33 1158 1843 2836 3027 33
Size: 1125 685 993 191 1121
Fragments arranged by size:
1125 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 150 1137 150
Size: 987 3128
SmlI C'TyrA_G
Cuts at: 933 1270 2185 2677 2939 933
Size: 337 915 492 262 2109
Fragments arranged by size:
2109 915 492 337 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 271 1168 1668 271
Size: 897 500 2718
Fragments arranged by size:
2718 897 500
StyI C'CwwG_G
Cuts at: 107 960 1013 1249 1603 107
Size: 853 53 236 354 2619
Fragments arranged by size:
2619 853 354 236 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1698 1831 2290 2499 1698
Size: 133 459 209 3314
Fragments arranged by size:
3314 459 209 133
TatI w'GTAC_w
Cuts at: 237 378 3630 237
Size: 141 3252 722
Fragments arranged by size:
3252 722 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 148 1135 148
Size: 987 3128
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
BaeI BbvCI BfrBI BglI BmgBI Bpu10I BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI