Restriction map for
pF1KE0412
(Circular) (Six-base) MAPSORT of: pF1KE0412.seq Check: 5153 from: 1 to: 3842
pF1KE0412 3842 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 767 858 767
Size: 91 3751
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
AflIII A'CryG_T
Cuts at: 451 1194 2298 3275 451
Size: 743 1104 977 1018
Fragments arranged by size:
1104 1018 977 743
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 1147 2714 1147
Size: 1567 2275
ApaI G_GGCC'C
Cuts at: 154 179 213 154
Size: 25 34 3783
Fragments arranged by size:
3783 34 25
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 862 862
Size: 3842
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 751 784 751
Size: 33 3809
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 767 858 1272 1520 1555 767
Size: 91 414 248 35 3054
Fragments arranged by size:
3054 414 248 91 35
BanII G_rGCy'C
Cuts at: 154 179 213 239 856 1886 154
Size: 25 34 26 617 1030 2110
Fragments arranged by size:
2110 1030 617 34 26 25
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbsI GAAGACnn'nnnn_
Cuts at: 768 768
Size: 3842
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 622 954 622
Size: 332 3510
Bme1580I G_kGCm'C
Cuts at: 154 179 205 213 498 1467 1560 2616 154
Size: 25 26 8 285 969 93 1056 1380
Fragments arranged by size:
1380 1056 969 285 93 26 25 8
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 568 3546 568
Size: 2978 864
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 167 1273 1521 2223 3719 167
Size: 1106 248 702 1496 290
Fragments arranged by size:
1496 1106 702 290 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 247 333 600 244
Size: 3 86 267 3486
Fragments arranged by size:
3486 267 86 3
BseYI C'CCAG_C
Cuts at: 172 376 824 1267 2025 2602 3098 172
Size: 204 448 443 758 577 496 916
Fragments arranged by size:
916 758 577 496 448 443 204
BsiEI CG_ry'CG
Cuts at: 104 161 1384 1430 2638 104
Size: 57 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 57 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 154 179 205 213 239 498 856 1467 1560
Size: 25 26 8 26 259 358 611 93
Cuts at: 1560 1634 1824 1886 2616 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 730 611 358 259 190 93 74
62 26 26 25 8
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 253 892 1106 1408 1789 253
Size: 639 214 302 381 2306
Fragments arranged by size:
2306 639 381 302 214
BsrBI CCG'CTC
Cuts at: 50 621 2134 2188 3672 3837 50
Size: 571 1513 54 1484 165 55
Fragments arranged by size:
1513 1484 571 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 156 257 1840 2021 2230 156
Size: 101 1583 181 209 1768
Fragments arranged by size:
1768 1583 209 181 101
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 161 311 524 107
Size: 54 150 213 3425
Fragments arranged by size:
3425 213 150 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 285 1792 1973 2136 285
Size: 1507 181 163 1991
Fragments arranged by size:
1991 1507 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 132 158 521 1427 1601 1992 2019 3364 132
Size: 26 363 906 174 391 27 1345 610
Fragments arranged by size:
1345 906 610 391 363 174 27 26
EagI C'GGCC_G
Cuts at: 158 1427 158
Size: 1269 2573
EarI CTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 150 209 210 629 820 981 150
Size: 59 1 419 191 161 3011
Fragments arranged by size:
3011 419 191 161 59 1
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 441 881 441
Size: 440 3402
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 771 862 771
Size: 91 3751
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 304 700 1831 1958 304
Size: 396 1131 127 2188
Fragments arranged by size:
2188 1131 396 127
MspA1I CmG'CkG
Cuts at: 163 172 267 288 953 1267 1627 2640 2885
Size: 9 95 21 665 314 360 1013 245
Cuts at: 2885 163
Size: 1120
Fragments arranged by size:
1120 1013 665 360 314 245 95 21
9
NaeI GCC'GGC
Cuts at: 158 2023 158
Size: 1865 1977
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 107 107
Size: 3842
NgoMIV G'CCGG_C
Cuts at: 156 2021 156
Size: 1865 1977
NspI r_CATG'y
Cuts at: 455 895 1395 2302 455
Size: 440 500 907 1995
Fragments arranged by size:
1995 907 500 440
PasI CC'CwG_GG
Cuts at: 294 498 294
Size: 204 3638
PciI A'CATG_T
Cuts at: 451 2298 451
Size: 1847 1995
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PpuMI rG'GwC_Cy
Cuts at: 629 629
Size: 3842
PspOMI G'GGCC_C
Cuts at: 150 175 209 150
Size: 25 34 3783
Fragments arranged by size:
3783 34 25
PstI C_TGCA'G
Cuts at: 287 889 1574 287
Size: 602 685 2555
Fragments arranged by size:
2555 685 602
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 288 1267 1627 288
Size: 979 360 2503
Fragments arranged by size:
2503 979 360
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SacII CC_GC'GG
Cuts at: 164 164
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SexAI A'CCwGG_T
Cuts at: 770 770
Size: 3842
SfcI C'TryA_G
Cuts at: 33 283 885 1570 2563 2754 33
Size: 250 602 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 250 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 107 215 976 1330 107
Size: 108 761 354 2619
Fragments arranged by size:
2619 761 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 775 1425 1558 2017 2226 775
Size: 650 133 459 209 2391
Fragments arranged by size:
2391 650 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI