Restriction map for
pF1KE0406
(Circular) (Six-base) MAPSORT of: pF1KE0406.seq Check: 7808 from: 1 to: 4136
pF1KE0406 4136 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 499 499
Size: 4136
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 468 1961 2393 3140 3408 448
Size: 20 1493 432 747 268 1176
Fragments arranged by size:
1493 1176 747 432 268 20
AflIII A'CryG_T
Cuts at: 449 1488 2592 3569 449
Size: 1039 1104 977 1016
Fragments arranged by size:
1104 1039 1016 977
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AlwNI CAG_nnn'CTG
Cuts at: 584 1441 3008 584
Size: 857 1567 1712
Fragments arranged by size:
1712 1567 857
ApaI G_GGCC'C
Cuts at: 494 494
Size: 4136
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 379 588 685 1140 3489 3745 379
Size: 209 97 455 2349 256 770
Fragments arranged by size:
2349 770 455 256 209 97
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 1156 1156
Size: 4136
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 499 1152 1566 1814 1849 499
Size: 653 414 248 35 2786
Fragments arranged by size:
2786 653 414 248 35
BanII G_rGCy'C
Cuts at: 494 1150 2180 494
Size: 656 1030 2450
Fragments arranged by size:
2450 1030 656
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 474 913 474
Size: 439 3697
BbvCI CC'TCA_GC
Cuts at: 504 504
Size: 4136
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4136
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 494 557 1761 1854 2910 494
Size: 63 1204 93 1056 1720
Fragments arranged by size:
1720 1204 1056 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 231 1348 1429 1764 3908 231
Size: 1117 81 335 2144 459
Fragments arranged by size:
2144 1117 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1092 2503 1092
Size: 1411 2725
Bpu10I CC'TnA_GC
Cuts at: 504 504
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 129 3840 129
Size: 3711 425
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 635 1650 3687 6
Size: 629 1015 2037 455
Fragments arranged by size:
2037 1015 629 455
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 693 1325 1846 2524 2798 2945 3356 693
Size: 632 521 678 274 147 411 1473
Fragments arranged by size:
1473 678 632 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 215
Size: 30 4106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 4136
BseYI C'CCAG_C
Cuts at: 358 521 1561 2319 2896 3392 358
Size: 163 1040 758 577 496 1102
Fragments arranged by size:
1102 1040 758 577 496 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 4136
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1150 1928 2118 2910 1150
Size: 778 190 792 2376
Fragments arranged by size:
2376 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 494 557 1150 1761 1854 1928 2118 2180 2910
Size: 63 593 611 93 74 190 62 730
Cuts at: 2910 494
Size: 1720
Fragments arranged by size:
1720 730 611 593 190 93 74 63
62
BspEI T'CCGG_A
Cuts at: 693 3356 693
Size: 2663 1473
BspHI T'CATG_A
Cuts at: 902 3312 902
Size: 2410 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 1186 1400 1702 2083 499
Size: 687 214 302 381 2552
Fragments arranged by size:
2552 687 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 1513
Size: 4136
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstXI CCAn_nnnn'nTGG
Cuts at: 244 981 244
Size: 737 3399
BstYI r'GATC_y
Cuts at: 1 1161 1628 1651 1986 2232 3244 3330 3342
Size: 1160 467 23 335 246 1012 86 12
Cuts at: 3342 3688 1
Size: 346 449
Fragments arranged by size:
1160 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1085 1085
Size: 4136
BtgI C'CryG_G
Cuts at: 107 107
Size: 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 555 795 821 1379 555
Size: 240 26 558 3312
Fragments arranged by size:
3312 558 240 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 770 805 770
Size: 35 4101
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 940 1721 1895 2286 2313 3658 940
Size: 781 174 391 27 1345 1418
Fragments arranged by size:
1418 1345 781 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 468 1961 2393 3140 3408 448
Size: 20 1493 432 747 268 1176
Fragments arranged by size:
1493 1176 747 432 268 20
EcoNI CCTnn'n_nnAGG
Cuts at: 145 145
Size: 4136
EcoO109I rG'GnC_Cy
Cuts at: 490 988 1275 490
Size: 498 287 3351
Fragments arranged by size:
3351 498 287
EcoRI G'AATT_C
Cuts at: 588 1140 588
Size: 552 3584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 4136
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 900 932 950 982 1978 2008 2010 2040 900
Size: 32 18 32 996 30 2 30 2996
Fragments arranged by size:
2996 996 32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 942 1897 942
Size: 955 3181
MslI CAynn'nnrTG
Cuts at: 2125 2252 2125
Size: 127 4009
MspA1I CmG'CkG
Cuts at: 433 1247 1561 1921 2934 3179 433
Size: 814 314 360 1013 245 1390
Fragments arranged by size:
1390 1013 814 360 314 245
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 107 107
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 453 1189 1689 2596 453
Size: 736 500 907 1993
Fragments arranged by size:
1993 907 736 500
PciI A'CATG_T
Cuts at: 449 2592 449
Size: 2143 1993
PflMI CCAn_nnn'nTGG
Cuts at: 949 949
Size: 4136
PfoI T'CCnGG_A
Cuts at: 1004 1004
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PpuMI rG'GwC_Cy
Cuts at: 988 988
Size: 4136
PsiI TTA'TAA
Cuts at: 424 424
Size: 4136
PspOMI G'GGCC_C
Cuts at: 490 490
Size: 4136
PstI C_TGCA'G
Cuts at: 813 1183 1868 813
Size: 370 685 3081
Fragments arranged by size:
3081 685 370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 433 1561 1921 433
Size: 1128 360 2648
Fragments arranged by size:
2648 1128 360
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SexAI A'CCwGG_T
Cuts at: 416 416
Size: 4136
SfcI C'TryA_G
Cuts at: 33 341 476 809 1179 1864 2857 3048 33
Size: 308 135 333 370 685 993 191 1121
Fragments arranged by size:
1121 993 685 370 333 308 191 135
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 1291 2206 2698 2960 1291
Size: 915 492 262 2467
Fragments arranged by size:
2467 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
SspI AAT'ATT
Cuts at: 1128 1128
Size: 4136
StyI C'CwwG_G
Cuts at: 107 1270 1624 107
Size: 1163 354 2619
Fragments arranged by size:
2619 1163 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1719 1852 2311 2520 1719
Size: 133 459 209 3335
Fragments arranged by size:
3335 459 209 133
TatI w'GTAC_w
Cuts at: 3651 3651
Size: 4136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1065 1167 1065
Size: 102 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 318 870 1631 318
Size: 552 761 2823
Fragments arranged by size:
2823 761 552
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI