Restriction map for
pF1KE0405
(Circular) (Six-base) MAPSORT of: pF1KE0405.seq Check: 3920 from: 1 to: 4007
pF1KE0405 4007 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 124 419 813 1045 124
Size: 295 394 232 3086
Fragments arranged by size:
3086 394 295 232
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1832 2264 3011 3279 814
Size: 1018 432 747 268 1542
Fragments arranged by size:
1542 1018 747 432 268
AflII C'TTAA_G
Cuts at: 540 540
Size: 4007
AflIII A'CryG_T
Cuts at: 385 816 1359 2463 3440 385
Size: 431 543 1104 977 952
Fragments arranged by size:
1104 977 952 543 431
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 564 1011 3360 3616 564
Size: 447 2349 256 955
Fragments arranged by size:
2349 955 447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 1023 1437 1685 1720 1023
Size: 414 248 35 3310
Fragments arranged by size:
3310 414 248 35
BanII G_rGCy'C
Cuts at: 1021 2051 1021
Size: 1030 2977
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BbsI GAAGACnn'nnnn_
Cuts at: 118 310 552 118
Size: 192 242 3573
Fragments arranged by size:
3573 242 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 918 952 918
Size: 34 3973
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BfrBI ATG'CAT
Cuts at: 730 730
Size: 4007
BglI GCCn_nnn'nGGC
Cuts at: 926 926
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 1632 1725 2781 1632
Size: 93 1056 2858
Fragments arranged by size:
2858 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1219 1300 1635 3779 1219
Size: 81 335 2144 1447
Fragments arranged by size:
2144 1447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 943
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 386 817 1360 386
Size: 431 543 3033
Fragments arranged by size:
3033 543 431
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 176 1432 2190 2767 3263 176
Size: 1256 758 577 496 920
Fragments arranged by size:
1256 920 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1021 1799 1989 2781 1021
Size: 778 190 792 2247
Fragments arranged by size:
2247 792 778 190
BsmI GAATG_Cn'
Cuts at: 730 730
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 1021 1632 1725 1799 1989 2051 2781 1021
Size: 611 93 74 190 62 730 2247
Fragments arranged by size:
2247 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1057 1271 1573 1954 1057
Size: 214 302 381 3110
Fragments arranged by size:
3110 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstYI r'GATC_y
Cuts at: 1 1032 1499 1522 1857 2103 3115 3201 3213
Size: 1031 467 23 335 246 1012 86 12
Cuts at: 3213 3559 1
Size: 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 420 420
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 120 1713 2571 120
Size: 1593 858 1556
Fragments arranged by size:
1593 1556 858
EaeI y'GGCC_r
Cuts at: 1592 1766 2157 2184 3529 1592
Size: 174 391 27 1345 2070
Fragments arranged by size:
2070 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 905 2030 2240 905
Size: 1125 210 2672
Fragments arranged by size:
2672 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 943 1832 2264 3011 3279 814
Size: 129 889 432 747 268 1542
Fragments arranged by size:
1542 889 747 432 268 129
EcoO109I rG'GnC_Cy
Cuts at: 1146 1146
Size: 4007
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 1441 1689 2711 3690 1441
Size: 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1849 1879 1881 1911 1849
Size: 30 2 30 3945
Fragments arranged by size:
3945 30 30 2
HincII GTy'rAC
Cuts at: 435 1046 435
Size: 611 3396
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 1768 1768
Size: 4007
MslI CAynn'nnrTG
Cuts at: 1996 2123 1996
Size: 127 3880
MspA1I CmG'CkG
Cuts at: 1118 1432 1792 2805 3050 1118
Size: 314 360 1013 245 2075
Fragments arranged by size:
2075 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NsiI A_TGCA'T
Cuts at: 732 732
Size: 4007
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PfoI T'CCnGG_A
Cuts at: 896 896
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PmlI CAC'GTG
Cuts at: 386 386
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PstI C_TGCA'G
Cuts at: 122 1054 1739 122
Size: 932 685 2390
Fragments arranged by size:
2390 932 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 118 1050 1735 2728 2919 33
Size: 85 932 685 993 191 1121
Fragments arranged by size:
1121 993 932 685 191 85
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 540 1162 2077 2569 2831 540
Size: 622 915 492 262 1716
Fragments arranged by size:
1716 915 622 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
SspI AAT'ATT
Cuts at: 276 276
Size: 4007
StyI C'CwwG_G
Cuts at: 1141 1495 1141
Size: 354 3653
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1590 1723 2182 2391 1590
Size: 133 459 209 3206
Fragments arranged by size:
3206 459 209 133
TatI w'GTAC_w
Cuts at: 3522 3522
Size: 4007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 3975
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI