Restriction map for
pF1KE0401
(Circular) (Six-base) MAPSORT of: pF1KE0401.seq Check: 4953 from: 1 to: 3728
pF1KE0401 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 428 1553 1985 2732 3000 259
Size: 169 1125 432 747 268 987
Fragments arranged by size:
1125 987 747 432 268 169
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AleI CACnn'nnGTG
Cuts at: 162 162
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 1033 2600 1033
Size: 1567 2161
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 117 753 117
Size: 636 3092
BanI G'GyrC_C
Cuts at: 744 1158 1406 1441 744
Size: 414 248 35 3031
Fragments arranged by size:
3031 414 248 35
BanII G_rGCy'C
Cuts at: 742 1772 742
Size: 1030 2698
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BciVI GTATCCnnnnn_n'
Cuts at: 654 1626 2387 3728 654
Size: 972 761 1341 654
Fragments arranged by size:
1341 972 761 654
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 132 271 940 1021 1356 3500 132
Size: 139 669 81 335 2144 360
Fragments arranged by size:
2144 669 360 335 139 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 645 442
Size: 203 3525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 382 1081 382
Size: 699 3029
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 346
Size: 30 3698
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3728
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 742 1353 1446 1520 1710 1772 2502 742
Size: 611 93 74 190 62 730 1968
Fragments arranged by size:
1968 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 317 2904 317
Size: 2587 1141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 1640 182
Size: 1458 2270
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstXI CCAn_nnnn'nTGG
Cuts at: 114 145 114
Size: 31 3697
BstYI r'GATC_y
Cuts at: 1 117 176 753 1220 1243 1578 1824 2836
Size: 116 59 577 467 23 335 246 1012
Cuts at: 2836 2922 2934 3280 1
Size: 86 12 346 449
Fragments arranged by size:
1012 577 467 449 346 335 246 116
86 59 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 1313 1487 1878 1905 3250 1313
Size: 174 391 27 1345 1791
Fragments arranged by size:
1791 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 530 1751 1961 530
Size: 1221 210 2297
Fragments arranged by size:
2297 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 428 442 645 1553 1985 2732 3000 259
Size: 169 14 203 908 432 747 268 987
Fragments arranged by size:
987 908 747 432 268 203 169 14
EcoO109I rG'GnC_Cy
Cuts at: 516 867 516
Size: 351 3377
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 1162 1410 2432 3411 1162
Size: 248 1022 979 1479
Fragments arranged by size:
1479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 575 767 575
Size: 192 3536
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 112 162 1717 1844 112
Size: 50 1555 127 1996
Fragments arranged by size:
1996 1555 127 50
MspA1I CmG'CkG
Cuts at: 372 839 1153 1513 2526 2771 372
Size: 467 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 467 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 171 781 1281 2188 171
Size: 610 500 907 1711
Fragments arranged by size:
1711 907 610 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 124 156 2867 2899 124
Size: 32 2711 32 953
Fragments arranged by size:
2711 953 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 635 667 635
Size: 32 3696
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 372 1153 1513 372
Size: 781 360 2587
Fragments arranged by size:
2587 781 360
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 530 1751 1961 530
Size: 1221 210 2297
Fragments arranged by size:
2297 1221 210
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 670 771 1456 2449 2640 33
Size: 637 101 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 191 101
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SnaBI TAC'GTA
Cuts at: 382 382
Size: 3728
SpeI A'CTAG_T
Cuts at: 67 619 67
Size: 552 3176
SphI G_CATG'C
Cuts at: 171 781 1281 171
Size: 610 500 2618
Fragments arranged by size:
2618 610 500
StyI C'CwwG_G
Cuts at: 862 1216 862
Size: 354 3374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1311 1444 1903 2112 1311
Size: 133 459 209 2927
Fragments arranged by size:
2927 459 209 133
TatI w'GTAC_w
Cuts at: 488 505 3243 488
Size: 17 2738 973
Fragments arranged by size:
2738 973 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
XmnI GAAnn'nnTTC
Cuts at: 446 446
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI