Restriction map for
pF1KE0395
(Circular) (Six-base) MAPSORT of: pF1KE0395.seq Check: 2630 from: 1 to: 6677
pF1KE0395 6677 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2780 2780
Size: 6677
AccI GT'mk_AC
Cuts at: 1643 3715 1643
Size: 2072 4605
Acc65I G'GTAC_C
Cuts at: 665 3693 665
Size: 3028 3649
AclI AA'CG_TT
Cuts at: 6517 6517
Size: 6677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 2533 4502 4934 5681 5949 559
Size: 1974 1969 432 747 268 1287
Fragments arranged by size:
1974 1969 1287 747 432 268
AfeI AGC'GCT
Cuts at: 1275 1275
Size: 6677
AflIII A'CryG_T
Cuts at: 2072 2687 4029 5133 6110 2072
Size: 615 1342 1104 977 2639
Fragments arranged by size:
2639 1342 1104 977 615
AgeI A'CCGG_T
Cuts at: 5065 5065
Size: 6677
AhdI GACnn_n'nnGTC
Cuts at: 2784 2784
Size: 6677
AleI CACnn'nnGTG
Cuts at: 126 126
Size: 6677
AlwNI CAG_nnn'CTG
Cuts at: 774 3462 3525 3982 5549 774
Size: 2688 63 457 1567 1902
Fragments arranged by size:
2688 1902 1567 457 63
ApaI G_GGCC'C
Cuts at: 1721 2102 2562 2900 1721
Size: 381 460 338 5498
Fragments arranged by size:
5498 460 381 338
ApaLI G'TGCA_C
Cuts at: 3652 5447 3652
Size: 1795 4882
ApoI r'AATT_y
Cuts at: 209 1612 2522 3060 3494 3513 3681 6030 6286
Size: 1403 910 538 434 19 168 2349 256
Cuts at: 6286 209
Size: 600
Fragments arranged by size:
2349 1403 910 600 538 434 256 168
19
AseI AT'TA_AT
Cuts at: 20 1309 20
Size: 1289 5388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
AvaI C'yCGr_G
Cuts at: 3191 3372 3697 3191
Size: 181 325 6171
Fragments arranged by size:
6171 325 181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1403 1436 1403
Size: 33 6644
BamHI G'GATC_C
Cuts at: 255 3702 255
Size: 3447 3230
BanI G'GyrC_C
Cuts at: 665 3693 4107 4355 4390 665
Size: 3028 414 248 35 2952
Fragments arranged by size:
3028 2952 414 248 35
BanII G_rGCy'C
Cuts at: 116 278 1721 1864 2102 2562 2900 3115 3691
Size: 162 1443 143 238 460 338 215 576
Cuts at: 3691 4721 116
Size: 1030 2072
Fragments arranged by size:
2072 1443 1030 576 460 338 238 215
162 143
BbeI G_GCGC'C
Cuts at: 4111 4359 4111
Size: 248 6429
BbsI GAAGACnn'nnnn_
Cuts at: 353 434 353
Size: 81 6596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 820 854 820
Size: 34 6643
BciVI GTATCCnnnnn_n'
Cuts at: 4575 5336 6677 4575
Size: 761 1341 4575
Fragments arranged by size:
4575 1341 761
BclI T'GATC_A
Cuts at: 2255 2814 4197 2255
Size: 559 1383 4735
Fragments arranged by size:
4735 1383 559
BglII A'GATC_T
Cuts at: 2826 4192 6229 2826
Size: 1366 2037 3274
Fragments arranged by size:
3274 2037 1366
BlpI GC'TnA_GC
Cuts at: 3789 3789
Size: 6677
Bme1580I G_kGCm'C
Cuts at: 1721 1815 2102 2562 2900 3656 4302 4395 5451
Size: 94 287 460 338 756 646 93 1056
Cuts at: 5451 1721
Size: 2947
Fragments arranged by size:
2947 1056 756 646 460 338 287 94
93
BmgBI CAC'GTC
Cuts at: 2370 2762 2370
Size: 392 6285
BmrI ACTGGGnnnn_n'
Cuts at: 648 1201 2105 2916 3653 3889 3970 4305 6449
Size: 553 904 811 737 236 81 335 2144
Cuts at: 6449 648
Size: 876
Fragments arranged by size:
2144 904 876 811 737 553 335 236
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 3261 5044 135
Size: 3126 1783 1768
Fragments arranged by size:
3126 1783 1768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 262 2205 3076 3175 3369 109
Size: 153 1943 871 99 194 3417
Fragments arranged by size:
3417 1943 871 194 153 99
Bpu10I CC'TnA_GC
Cuts at: 1562 1562
Size: 6677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 2017 3602 3853 4732 5224 5522 574
Size: 1443 1585 251 879 492 298 1729
Fragments arranged by size:
1729 1585 1443 879 492 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 3231 6381 3231
Size: 3150 3527
BsaAI yAC'GTr
Cuts at: 4030 4030
Size: 6677
BsaBI GATnn'nnATC
Cuts at: 6 4191 6228 6
Size: 4185 2037 455
Fragments arranged by size:
4185 2037 455
BsaHI Gr'CG_yC
Cuts at: 4108 4356 5058 6554 4108
Size: 248 702 1496 4231
Fragments arranged by size:
4231 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3101 3866 4387 5065 5339 5486 5897 3101
Size: 765 521 678 274 147 411 3881
Fragments arranged by size:
3881 765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1715 1745 3351 3381 1715
Size: 30 1606 30 5011
Fragments arranged by size:
5011 1606 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1714 1733 3318 1714
Size: 19 1585 5073
Fragments arranged by size:
5073 1585 19
BseYI C'CCAG_C
Cuts at: 1572 1894 2004 3397 3638 4102 4860 5437 5933
Size: 322 110 1393 241 464 758 577 496
Cuts at: 5933 1572
Size: 2316
Fragments arranged by size:
2316 1393 758 577 496 464 322 241
110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1077 1968 2752 3082 1077
Size: 891 784 330 4672
Fragments arranged by size:
4672 891 784 330
BsiEI CG_ry'CG
Cuts at: 104 3139 4219 4265 5473 104
Size: 3035 1080 46 1208 1308
Fragments arranged by size:
3035 1308 1208 1080 46
BsiHKAI G_wGCw'C
Cuts at: 278 1270 1743 2645 3656 3691 4469 4659 5451
Size: 992 473 902 1011 35 778 190 792
Cuts at: 5451 278
Size: 1504
Fragments arranged by size:
1504 1011 992 902 792 778 473 190
35
BsmI GAATG_Cn'
Cuts at: 3288 3288
Size: 6677
BsmBI CGTCTCn'nnnn_
Cuts at: 1930 1930
Size: 6677
Bsp1286I G_dGCh'C
Cuts at: 116 278 1270 1721 1743 1815 1864 2102 2562
Size: 162 992 451 22 72 49 238 460
Cuts at: 2562 2645 2900 3115 3656 3691 4302 4395 4469
Size: 83 255 215 541 35 611 93 74
Cuts at: 4469 4659 4721 5451 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 992 730 611 541 460 451 255
238 215 190 162 93 83 74 72
62 49 35 22
BspEI T'CCGG_A
Cuts at: 5897 5897
Size: 6677
BspHI T'CATG_A
Cuts at: 5853 5853
Size: 6677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2780 3727 3941 4243 4624 2780
Size: 947 214 302 381 4833
Fragments arranged by size:
4833 947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 671 4969 5023 6507 6672 50
Size: 621 4298 54 1484 165 55
Fragments arranged by size:
4298 1484 621 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 743 1973 4589 392
Size: 351 1230 2616 2480
Fragments arranged by size:
2616 2480 1230 351
BsrFI r'CCGG_y
Cuts at: 305 662 2348 2860 4675 4856 5065 305
Size: 357 1686 512 1815 181 209 1917
Fragments arranged by size:
1917 1815 1686 512 357 209 181
BsrGI T'GTAC_A
Cuts at: 2690 3527 2690
Size: 837 5840
BssSI C'ACGA_G
Cuts at: 4948 5306 4948
Size: 358 6319
BstAPI GCAn_nnn'nTGC
Cuts at: 4054 4054
Size: 6677
BstBI TT'CG_AA
Cuts at: 5038 5038
Size: 6677
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2206
Size: 6677
BstYI r'GATC_y
Cuts at: 1 255 770 2451 2826 3702 4169 4192 4527
Size: 254 515 1681 375 876 467 23 335
Cuts at: 4527 4773 5785 5871 5883 6229 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1681 1012 876 515 467 449 375 346
335 254 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 375 1961 2115 2316 107
Size: 268 1586 154 201 4468
Fragments arranged by size:
4468 1586 268 201 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 845 4627 4808 4971 845
Size: 3782 181 163 2551
Fragments arranged by size:
3782 2551 181 163
BtsI GCAGTG_nn'
Cuts at: 755 2016 3203 3920 755
Size: 1261 1187 717 3512
Fragments arranged by size:
3512 1261 1187 717
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2234 2269 2234
Size: 35 6642
DraI TTT'AAA
Cuts at: 1149 3676 1149
Size: 2527 4150
DraIII CAC_nnn'GTG
Cuts at: 145 3048 145
Size: 2903 3774
DrdI GACnn_nn'nnGTC
Cuts at: 2506 4383 5241 2506
Size: 1877 858 3942
Fragments arranged by size:
3942 1877 858
EaeI y'GGCC_r
Cuts at: 2177 2319 2350 3136 4262 4436 4827 4854 6199
Size: 142 31 786 1126 174 391 27 1345
Cuts at: 6199 2177
Size: 2655
Fragments arranged by size:
2655 1345 1126 786 391 174 142 31
27
EagI C'GGCC_G
Cuts at: 3136 4262 3136
Size: 1126 5551
EarI CTCTTCn'nnn_
Cuts at: 287 885 1046 2393 4700 4910 287
Size: 598 161 1347 2307 210 2054
Fragments arranged by size:
2307 2054 1347 598 210 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3314 4885 5195 5341 6487 3314
Size: 1571 310 146 1146 3504
Fragments arranged by size:
3504 1571 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 3689 276
Size: 3413 3264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 262 559 2205 2533 3076 3175 3369 4502
Size: 153 297 1646 328 543 99 194 1133
Cuts at: 4502 4934 5681 5949 109
Size: 432 747 268 837
Fragments arranged by size:
1646 1133 837 747 543 432 328 297
268 194 153 99
EcoNI CCTnn'n_nnAGG
Cuts at: 510 610 510
Size: 100 6577
EcoO109I rG'GnC_Cy
Cuts at: 1717 2049 2558 2559 2868 2896 2897 2998 3573
Size: 332 509 1 309 28 1 101 575
Cuts at: 3573 3816 1717
Size: 243 4578
Fragments arranged by size:
4578 575 509 332 309 243 101 28
1 1
EcoRI G'AATT_C
Cuts at: 3681 3681
Size: 6677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4696 4696
Size: 6677
FspI TGC'GCA
Cuts at: 4458 4458
Size: 6677
HaeII r_GCGC'y
Cuts at: 1277 4111 4359 5381 6360 1277
Size: 2834 248 1022 979 1594
Fragments arranged by size:
2834 1594 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 955 987 1495 1527 2587 2619 2770
Size: 32 238 32 508 32 1060 32 151
Cuts at: 2770 2802 3501 3533 4519 4549 4551 4581 685
Size: 32 699 32 986 30 2 30 2781
Fragments arranged by size:
2781 1060 986 699 508 238 151 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 554 3716 554
Size: 3162 3515
HindIII A'AGCT_T
Cuts at: 421 2188 3316 421
Size: 1767 1128 3782
Fragments arranged by size:
3782 1767 1128
KasI G'GCGC_C
Cuts at: 4107 4355 4107
Size: 248 6429
KpnI G_GTAC'C
Cuts at: 669 3697 669
Size: 3028 3649
MluI A'CGCG_T
Cuts at: 6110 6110
Size: 6677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 1703 5348 5532 944
Size: 759 3645 184 2089
Fragments arranged by size:
3645 2089 759 184
MscI TGG'CCA
Cuts at: 2179 2321 4438 2179
Size: 142 2117 4418
Fragments arranged by size:
4418 2117 142
MslI CAynn'nnrTG
Cuts at: 126 748 1238 2989 4666 4793 126
Size: 622 490 1751 1677 127 2010
Fragments arranged by size:
2010 1751 1677 622 490 127
MspA1I CmG'CkG
Cuts at: 282 1920 2004 2462 3525 3642 3788 4102 4462
Size: 1638 84 458 1063 117 146 314 360
Cuts at: 4462 5475 5720 282
Size: 1013 245 1239
Fragments arranged by size:
1638 1239 1063 1013 458 360 314 245
146 117 84
NaeI GCC'GGC
Cuts at: 307 2350 4858 307
Size: 2043 2508 2126
Fragments arranged by size:
2508 2126 2043
NarI GG'CG_CC
Cuts at: 4108 4356 4108
Size: 248 6429
NcoI C'CATG_G
Cuts at: 107 375 1961 2115 2316 107
Size: 268 1586 154 201 4468
Fragments arranged by size:
4468 1586 268 201 154
NdeI CA'TA_TG
Cuts at: 1320 2336 3567 1320
Size: 1016 1231 4430
Fragments arranged by size:
4430 1231 1016
NgoMIV G'CCGG_C
Cuts at: 305 2348 4856 305
Size: 2043 2508 2126
Fragments arranged by size:
2508 2126 2043
NspI r_CATG'y
Cuts at: 2076 2580 2691 3730 4230 5137 2076
Size: 504 111 1039 500 907 3616
Fragments arranged by size:
3616 1039 907 504 500 111
PasI CC'CwG_GG
Cuts at: 357 735 1777 357
Size: 378 1042 5257
Fragments arranged by size:
5257 1042 378
PciI A'CATG_T
Cuts at: 2072 2687 5133 2072
Size: 615 2446 3616
Fragments arranged by size:
3616 2446 615
PfoI T'CCnGG_A
Cuts at: 689 3355 689
Size: 2666 4011
PmeI GTTT'AAAC
Cuts at: 3676 3676
Size: 6677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5816 5848 5816
Size: 32 6645
PpuMI rG'GwC_Cy
Cuts at: 2049 2868 3573 2049
Size: 819 705 5153
Fragments arranged by size:
5153 819 705
PsiI TTA'TAA
Cuts at: 1094 1094
Size: 6677
PspOMI G'GGCC_C
Cuts at: 1717 2098 2558 2896 1717
Size: 381 460 338 5498
Fragments arranged by size:
5498 460 381 338
PstI C_TGCA'G
Cuts at: 2467 3419 3724 4409 2467
Size: 952 305 685 4735
Fragments arranged by size:
4735 952 685 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6677
PvuII CAG'CTG
Cuts at: 282 2004 3525 3642 4102 4462 282
Size: 1722 1521 117 460 360 2497
Fragments arranged by size:
2497 1722 1521 460 360 117
RsrII CG'GwC_CG
Cuts at: 4872 4872
Size: 6677
SacI G_AGCT'C
Cuts at: 278 3691 278
Size: 3413 3264
SalI G'TCGA_C
Cuts at: 3714 3714
Size: 6677
SapI GCTCTTCn'nnn_
Cuts at: 4700 4910 4700
Size: 210 6467
SbfI CC_TGCA'GG
Cuts at: 3724 3724
Size: 6677
ScaI AGT'ACT
Cuts at: 658 3421 6194 658
Size: 2763 2773 1141
Fragments arranged by size:
2773 2763 1141
SexAI A'CCwGG_T
Cuts at: 431 431
Size: 6677
SfcI C'TryA_G
Cuts at: 33 2463 2628 2822 3415 3554 3720 4405 5398
Size: 2430 165 194 593 139 166 685 993
Cuts at: 5398 5589 33
Size: 191 1121
Fragments arranged by size:
2430 1121 993 685 593 194 191 166
165 139
SfoI GGC'GCC
Cuts at: 4109 4357 4109
Size: 248 6429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
SmaI CCC'GGG
Cuts at: 3193 3699 3193
Size: 506 6171
SmlI C'TyrA_G
Cuts at: 589 2032 3617 3832 4747 5239 5501 589
Size: 1443 1585 215 915 492 262 1765
Fragments arranged by size:
1765 1585 1443 915 492 262 215
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6677
SphI G_CATG'C
Cuts at: 3730 4230 3730
Size: 500 6177
SrfI GCCC'GGGC
Cuts at: 3193 3193
Size: 6677
StuI AGG'CCT
Cuts at: 505 3244 505
Size: 2739 3938
StyI C'CwwG_G
Cuts at: 107 219 375 506 950 1671 1769 1961 2115
Size: 112 156 131 444 721 98 192 154
Cuts at: 2115 2316 3218 3811 4165 107
Size: 201 902 593 354 2619
Fragments arranged by size:
2619 902 721 593 444 354 201 192
156 154 131 112 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2997 3104 4260 4393 4852 5061 2997
Size: 107 1156 133 459 209 4613
Fragments arranged by size:
4613 1156 459 209 133 107
TatI w'GTAC_w
Cuts at: 542 656 776 2690 3419 3527 3661 6192 542
Size: 114 120 1914 729 108 134 2531 1027
Fragments arranged by size:
2531 1914 1027 729 134 120 114 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1715 1747 2275 2307 6194 6226 1715
Size: 32 528 32 3887 32 2166
Fragments arranged by size:
3887 2166 528 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 433 2991 4474 433
Size: 2558 1483 2636
Fragments arranged by size:
2636 2558 1483
XbaI T'CTAG_A
Cuts at: 3708 3708
Size: 6677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 3666 4172 2314
Size: 1352 506 4819
Fragments arranged by size:
4819 1352 506
XmaI C'CCGG_G
Cuts at: 3191 3697 3191
Size: 506 6171
XmnI GAAnn'nnTTC
Cuts at: 894 894
Size: 6677
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BbvCI BfrBI
BglI BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SspI SwaI XhoI ZraI