Restriction map for
pF1KE0393
(Circular) (Six-base) MAPSORT of: pF1KE0393.seq Check: 7511 from: 1 to: 5978
pF1KE0393 5978 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 754 2371 754
Size: 1617 4361
AatII G_ACGT'C
Cuts at: 2834 2834
Size: 5978
AccI GT'mk_AC
Cuts at: 170 248 2117 3016 170
Size: 78 1869 899 3132
Fragments arranged by size:
3132 1869 899 78
Acc65I G'GTAC_C
Cuts at: 2994 2994
Size: 5978
AclI AA'CG_TT
Cuts at: 5818 5818
Size: 5978
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 730 853 980 1186 1311 2860 3803 4235
Size: 6 123 127 206 125 1549 943 432
Cuts at: 4235 4982 5250 724
Size: 747 268 1452
Fragments arranged by size:
1549 1452 943 747 432 268 206 127
125 123 6
AflIII A'CryG_T
Cuts at: 767 2516 2624 3330 4434 5411 767
Size: 1749 108 706 1104 977 1334
Fragments arranged by size:
1749 1334 1104 977 706 108
AgeI A'CCGG_T
Cuts at: 4366 4366
Size: 5978
AhdI GACnn_n'nnGTC
Cuts at: 166 166
Size: 5978
AleI CACnn'nnGTG
Cuts at: 1328 2931 1328
Size: 1603 4375
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 606 638 606
Size: 32 5946
AlwNI CAG_nnn'CTG
Cuts at: 492 894 1164 1761 1851 2217 2406 3283 4850
Size: 402 270 597 90 366 189 877 1567
Cuts at: 4850 492
Size: 1620
Fragments arranged by size:
1620 1567 877 597 402 366 270 189
90
ApaI G_GGCC'C
Cuts at: 2768 2942 2768
Size: 174 5804
ApaLI G'TGCA_C
Cuts at: 514 4748 514
Size: 4234 1744
ApoI r'AATT_y
Cuts at: 2982 5331 5587 2982
Size: 2349 256 3373
Fragments arranged by size:
3373 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5978
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
AvaI C'yCGr_G
Cuts at: 1007 1566 2540 2799 2998 1007
Size: 559 974 259 199 3987
Fragments arranged by size:
3987 974 559 259 199
BamHI G'GATC_C
Cuts at: 2871 3003 2871
Size: 132 5846
BanI G'GyrC_C
Cuts at: 299 453 819 979 1339 1371 1887 2497 2994
Size: 154 366 160 360 32 516 610 497
Cuts at: 2994 3408 3656 3691 299
Size: 414 248 35 2586
Fragments arranged by size:
2586 610 516 497 414 366 360 248
160 154 35 32
BanII G_rGCy'C
Cuts at: 674 1313 1736 1975 2108 2333 2685 2768 2910
Size: 639 423 239 133 225 352 83 142
Cuts at: 2910 2942 2992 4022 674
Size: 32 50 1030 2630
Fragments arranged by size:
2630 1030 639 423 352 239 225 142
133 83 50 32
BbeI G_GCGC'C
Cuts at: 1891 3412 3660 1891
Size: 1521 248 4209
Fragments arranged by size:
4209 1521 248
BbsI GAAGACnn'nnnn_
Cuts at: 565 1072 565
Size: 507 5471
BbvCI CC'TCA_GC
Cuts at: 985 1387 985
Size: 402 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 382 416 382
Size: 34 5944
BciVI GTATCCnnnnn_n'
Cuts at: 3876 4637 5978 3876
Size: 761 1341 3876
Fragments arranged by size:
3876 1341 761
BclI T'GATC_A
Cuts at: 1127 3498 1127
Size: 2371 3607
BglI GCCn_nnn'nGGC
Cuts at: 1569 1885 1569
Size: 316 5662
BglII A'GATC_T
Cuts at: 2384 3493 5530 2384
Size: 1109 2037 2832
Fragments arranged by size:
2832 2037 1109
BlpI GC'TnA_GC
Cuts at: 3090 3090
Size: 5978
Bme1580I G_kGCm'C
Cuts at: 302 518 1196 1374 1382 1442 2249 2750 2768
Size: 216 678 178 8 60 807 501 18
Cuts at: 2768 2942 3603 3696 4752 302
Size: 174 661 93 1056 1528
Fragments arranged by size:
1528 1056 807 678 661 501 216 178
174 93 60 18 8
BmgBI CAC'GTC
Cuts at: 483 1125 1749 483
Size: 642 624 4712
Fragments arranged by size:
4712 642 624
BmrI ACTGGGnnnn_n'
Cuts at: 1730 2753 3190 3271 3606 5750 1730
Size: 1023 437 81 335 2144 1958
Fragments arranged by size:
2144 1958 1023 437 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4345 4345
Size: 5978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1600 1630 2239 161
Size: 1439 30 609 3900
Fragments arranged by size:
3900 1439 609 30
Bpu10I CC'TnA_GC
Cuts at: 985 1280 1387 985
Size: 295 107 5576
Fragments arranged by size:
5576 295 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 4033 4525 4823 3154
Size: 879 492 298 4309
Fragments arranged by size:
4309 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 685 2001 2708 5682 685
Size: 1316 707 2974 981
Fragments arranged by size:
2974 1316 981 707
BsaAI yAC'GTr
Cuts at: 1182 2082 3331 1182
Size: 900 1249 3829
Fragments arranged by size:
3829 1249 900
BsaBI GATnn'nnATC
Cuts at: 6 3492 5529 6
Size: 3486 2037 455
Fragments arranged by size:
3486 2037 455
BsaHI Gr'CG_yC
Cuts at: 1888 1916 2831 3409 3657 4359 5855 1888
Size: 28 915 578 248 702 1496 2011
Fragments arranged by size:
2011 1496 915 702 578 248 28
BsaWI w'CCGG_w
Cuts at: 1590 3167 3688 4366 4640 4787 5198 1590
Size: 1577 521 678 274 147 411 2370
Fragments arranged by size:
2370 1577 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2290 2320 2593 2623 2629 2659 2290
Size: 30 273 30 6 30 5609
Fragments arranged by size:
5609 273 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1398 1597 1398
Size: 199 5779
BseYI C'CCAG_C
Cuts at: 920 1152 1761 1808 2547 3403 4161 4738 5234
Size: 232 609 47 739 856 758 577 496
Cuts at: 5234 920
Size: 1664
Fragments arranged by size:
1664 856 758 739 609 577 496 232
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 907 1014 1021 1306 1538 1720 1732 1971
Size: 402 107 7 285 232 182 12 239
Cuts at: 1971 505
Size: 4512
Fragments arranged by size:
4512 402 285 239 232 182 107 12
7
BsiEI CG_ry'CG
Cuts at: 104 420 1519 3520 3566 4774 104
Size: 316 1099 2001 46 1208 1308
Fragments arranged by size:
2001 1308 1208 1099 316 46
BsiHKAI G_wGCw'C
Cuts at: 518 966 1313 1529 1778 2129 2992 3770 3960
Size: 448 347 216 249 351 863 778 190
Cuts at: 3960 4752 518
Size: 792 1744
Fragments arranged by size:
1744 863 792 778 448 351 347 249
216 190
BsmI GAATG_Cn'
Cuts at: 2442 2442
Size: 5978
BsmBI CGTCTCn'nnnn_
Cuts at: 373 1962 373
Size: 1589 4389
Bsp1286I G_dGCh'C
Cuts at: 302 518 674 966 1196 1313 1374 1382 1442
Size: 216 156 292 230 117 61 8 60
Cuts at: 1442 1529 1736 1778 1975 2108 2129 2249 2333
Size: 87 207 42 197 133 21 120 84
Cuts at: 2333 2685 2750 2768 2910 2942 2992 3603 3696
Size: 352 65 18 142 32 50 611 93
Cuts at: 3696 3770 3960 4022 4752 302
Size: 74 190 62 730 1528
Fragments arranged by size:
1528 730 611 352 292 230 216 207
197 190 156 142 133 120 117 93
87 84 74 65 62 61 60 50
42 32 21 18 8
BspEI T'CCGG_A
Cuts at: 1590 5198 1590
Size: 3608 2370
BspHI T'CATG_A
Cuts at: 5154 5154
Size: 5978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 325 725 754 1085 1328 2371 3028 3242
Size: 198 400 29 331 243 1043 657 214
Cuts at: 3242 3544 3925 127
Size: 302 381 2180
Fragments arranged by size:
2180 1043 657 400 381 331 302 243
214 198 29
BsrBI CCG'CTC
Cuts at: 50 1634 4270 4324 5808 5973 50
Size: 1584 2636 54 1484 165 55
Fragments arranged by size:
2636 1584 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1278 1498 2730 3890 1278
Size: 220 1232 1160 3366
Fragments arranged by size:
3366 1232 1160 220
BsrFI r'CCGG_y
Cuts at: 456 1236 1766 3976 4157 4366 456
Size: 780 530 2210 181 209 2068
Fragments arranged by size:
2210 2068 780 530 209 181
BssSI C'ACGA_G
Cuts at: 4249 4607 4249
Size: 358 5620
BstAPI GCAn_nnn'nTGC
Cuts at: 195 894 1851 3355 195
Size: 699 957 1504 2818
Fragments arranged by size:
2818 1504 957 699
BstBI TT'CG_AA
Cuts at: 4339 4339
Size: 5978
BstEII G'GTnAC_C
Cuts at: 114 1206 1263 1458 114
Size: 1092 57 195 4634
Fragments arranged by size:
4634 1092 195 57
BstXI CCAn_nnnn'nTGG
Cuts at: 477 652 943 1047 1529 1968 2883 2933 477
Size: 175 291 104 482 439 915 50 3522
Fragments arranged by size:
3522 915 482 439 291 175 104 50
BstYI r'GATC_y
Cuts at: 1 2384 2871 3003 3470 3493 3828 4074 5086
Size: 2383 487 132 467 23 335 246 1012
Cuts at: 5086 5172 5184 5530 1
Size: 86 12 346 449
Fragments arranged by size:
2383 1012 487 467 449 346 335 246
132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2504 2504
Size: 5978
BtgI C'CryG_G
Cuts at: 107 815 936 1454 1513 2942 107
Size: 708 121 518 59 1429 3143
Fragments arranged by size:
3143 1429 708 518 121 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 545 1800 3928 4109 4272 545
Size: 1255 2128 181 163 2251
Fragments arranged by size:
2251 2128 1255 181 163
BtsI GCAGTG_nn'
Cuts at: 150 289 895 934 949 1196 1555 2095 2907
Size: 139 606 39 15 247 359 540 812
Cuts at: 2907 3221 150
Size: 314 2907
Fragments arranged by size:
2907 812 606 540 359 314 247 139
39 15
DraI TTT'AAA
Cuts at: 2977 2977
Size: 5978
DrdI GACnn_nn'nnGTC
Cuts at: 1390 3684 4542 1390
Size: 2294 858 2826
Fragments arranged by size:
2826 2294 858
EaeI y'GGCC_r
Cuts at: 417 458 1516 2945 3563 3737 4128 4155 5500
Size: 41 1058 1429 618 174 391 27 1345
Cuts at: 5500 417
Size: 895
Fragments arranged by size:
1429 1345 1058 895 618 391 174 41
27
EagI C'GGCC_G
Cuts at: 417 1516 3563 417
Size: 1099 2047 2832
Fragments arranged by size:
2832 2047 1099
EarI CTCTTCn'nnn_
Cuts at: 1993 4001 4211 1993
Size: 2008 210 3760
Fragments arranged by size:
3760 2008 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1989 2137 2254 4186 4496 4642 5788 1989
Size: 148 117 1932 310 146 1146 2179
Fragments arranged by size:
2179 1932 1146 310 148 146 117
EcoICRI GAG'CTC
Cuts at: 1311 2990 1311
Size: 1679 4299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 724 730 853 980 1186 1311 1600 1630
Size: 563 6 123 127 206 125 289 30
Cuts at: 1630 2239 2860 3803 4235 4982 5250 161
Size: 609 621 943 432 747 268 889
Fragments arranged by size:
943 889 747 621 609 563 432 289
268 206 127 125 123 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1990 1990
Size: 5978
EcoO109I rG'GnC_Cy
Cuts at: 699 1161 1637 2403 2765 2938 3117 699
Size: 462 476 766 362 173 179 3560
Fragments arranged by size:
3560 766 476 462 362 179 173
EcoRI G'AATT_C
Cuts at: 2982 2982
Size: 5978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 5978
FseI GG_CCGG'CC
Cuts at: 1770 1770
Size: 5978
FspI TGC'GCA
Cuts at: 388 3759 388
Size: 3371 2607
HaeII r_GCGC'y
Cuts at: 139 344 1762 1891 2594 3412 3660 4682 5661
Size: 205 1418 129 703 818 248 1022 979
Cuts at: 5661 139
Size: 456
Fragments arranged by size:
1418 1022 979 818 703 456 248 205
129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 619 651 1957 1989 2129 2161 2290
Size: 32 435 32 1306 32 140 32 129
Cuts at: 2290 2322 3820 3850 3852 3882 152
Size: 32 1498 30 2 30 2248
Fragments arranged by size:
2248 1498 1306 435 140 129 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 585 660 1074 1401 2118 3017 585
Size: 75 414 327 717 899 3546
Fragments arranged by size:
3546 899 717 414 327 75
KasI G'GCGC_C
Cuts at: 1887 3408 3656 1887
Size: 1521 248 4209
Fragments arranged by size:
4209 1521 248
KpnI G_GTAC'C
Cuts at: 2998 2998
Size: 5978
MluI A'CGCG_T
Cuts at: 5411 5411
Size: 5978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 614 1985 4649 4833 614
Size: 1371 2664 184 1759
Fragments arranged by size:
2664 1759 1371 184
MscI TGG'CCA
Cuts at: 2947 3739 2947
Size: 792 5186
MslI CAynn'nnrTG
Cuts at: 112 274 475 1184 1328 1435 2255 2931 3967
Size: 162 201 709 144 107 820 676 1036
Cuts at: 3967 4094 112
Size: 127 1996
Fragments arranged by size:
1996 1036 820 709 676 201 162 144
127 107
MspA1I CmG'CkG
Cuts at: 924 1152 1515 1646 1704 1848 1937 2214 2931
Size: 228 363 131 58 144 89 277 717
Cuts at: 2931 3089 3403 3763 4776 5021 924
Size: 158 314 360 1013 245 1881
Fragments arranged by size:
1881 1013 717 363 360 314 277 245
228 158 144 131 89 58
NaeI GCC'GGC
Cuts at: 458 1238 1768 4159 458
Size: 780 530 2391 2277
Fragments arranged by size:
2391 2277 780 530
NarI GG'CG_CC
Cuts at: 1888 3409 3657 1888
Size: 1521 248 4209
Fragments arranged by size:
4209 1521 248
NcoI C'CATG_G
Cuts at: 107 936 2942 107
Size: 829 2006 3143
Fragments arranged by size:
3143 2006 829
NgoMIV G'CCGG_C
Cuts at: 456 1236 1766 4157 456
Size: 780 530 2391 2277
Fragments arranged by size:
2391 2277 780 530
NotI GC'GGCC_GC
Cuts at: 417 1516 417
Size: 1099 4879
NruI TCG'CGA
Cuts at: 1928 1928
Size: 5978
NspI r_CATG'y
Cuts at: 202 215 1248 2520 2628 3031 3531 4438 202
Size: 13 1033 1272 108 403 500 907 1742
Fragments arranged by size:
1742 1272 1033 907 500 403 108 13
PasI CC'CwG_GG
Cuts at: 868 957 972 1366 868
Size: 89 15 394 5480
Fragments arranged by size:
5480 394 89 15
PciI A'CATG_T
Cuts at: 2516 2624 4434 2516
Size: 108 1810 4060
Fragments arranged by size:
4060 1810 108
PflMI CCAn_nnn'nTGG
Cuts at: 1761 1761
Size: 5978
PfoI T'CCnGG_A
Cuts at: 257 356 2668 257
Size: 99 2312 3567
Fragments arranged by size:
3567 2312 99
PmeI GTTT'AAAC
Cuts at: 2977 2977
Size: 5978
PmlI CAC'GTG
Cuts at: 1182 1182
Size: 5978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 5117
Size: 32 5946
PpuMI rG'GwC_Cy
Cuts at: 699 1161 1637 2403 699
Size: 462 476 766 4274
Fragments arranged by size:
4274 766 476 462
PshAI GACnn'nnGTC
Cuts at: 697 697
Size: 5978
PspOMI G'GGCC_C
Cuts at: 2764 2938 2764
Size: 174 5804
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2062 2094 2062
Size: 32 5946
PstI C_TGCA'G
Cuts at: 284 929 2090 3025 3710 284
Size: 645 1161 935 685 2552
Fragments arranged by size:
2552 1161 935 685 645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5978
PvuII CAG'CTG
Cuts at: 924 1152 1704 1848 2214 2931 3403 3763 924
Size: 228 552 144 366 717 472 360 3139
Fragments arranged by size:
3139 717 552 472 366 360 228 144
RsrII CG'GwC_CG
Cuts at: 2190 4173 2190
Size: 1983 3995
SacI G_AGCT'C
Cuts at: 1313 2992 1313
Size: 1679 4299
SacII CC_GC'GG
Cuts at: 1516 1516
Size: 5978
SalI G'TCGA_C
Cuts at: 2116 3015 2116
Size: 899 5079
SapI GCTCTTCn'nnn_
Cuts at: 4001 4211 4001
Size: 210 5768
SbfI CC_TGCA'GG
Cuts at: 3025 3025
Size: 5978
ScaI AGT'ACT
Cuts at: 5495 5495
Size: 5978
SfcI C'TryA_G
Cuts at: 33 280 925 2010 2086 2133 3021 3706 4699
Size: 247 645 1085 76 47 888 685 993
Cuts at: 4699 4890 33
Size: 191 1121
Fragments arranged by size:
1121 1085 993 888 685 645 247 191
76 47
SfoI GGC'GCC
Cuts at: 1889 3410 3658 1889
Size: 1521 248 4209
Fragments arranged by size:
4209 1521 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
SmaI CCC'GGG
Cuts at: 1009 2801 3000 1009
Size: 1792 199 3987
Fragments arranged by size:
3987 1792 199
SmlI C'TyrA_G
Cuts at: 3133 4048 4540 4802 3133
Size: 915 492 262 4309
Fragments arranged by size:
4309 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5978
SphI G_CATG'C
Cuts at: 202 3031 3531 202
Size: 2829 500 2649
Fragments arranged by size:
2829 2649 500
SrfI GCCC'GGGC
Cuts at: 2801 2801
Size: 5978
StuI AGG'CCT
Cuts at: 2538 2538
Size: 5978
StyI C'CwwG_G
Cuts at: 107 695 936 1442 2942 3112 3466 107
Size: 588 241 506 1500 170 354 2619
Fragments arranged by size:
2619 1500 588 506 354 241 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 2248 2461 3561 3694 4153 4362 949
Size: 1299 213 1100 133 459 209 2565
Fragments arranged by size:
2565 1299 1100 459 213 209 133
TatI w'GTAC_w
Cuts at: 577 2222 5493 577
Size: 1645 3271 1062
Fragments arranged by size:
3271 1645 1062
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2047 2079 2629 2661 5495 5527 2047
Size: 32 550 32 2834 32 2498
Fragments arranged by size:
2834 2498 550 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 3775 2113
Size: 1662 4316
XbaI T'CTAG_A
Cuts at: 3009 3009
Size: 5978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1044 1257 2883 2955 3473 1044
Size: 213 1626 72 518 3549
Fragments arranged by size:
3549 1626 518 213 72
XmaI C'CCGG_G
Cuts at: 1007 2799 2998 1007
Size: 1792 199 3987
Fragments arranged by size:
3987 1792 199
ZraI GAC'GTC
Cuts at: 2832 2832
Size: 5978
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AlfI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NsiI PacI
PsiI PspXI SanDI SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI