Restriction map for
pF1KE0391
(Circular) (Six-base) MAPSORT of: pF1KE0391.seq Check: 1981 from: 1 to: 6704
pF1KE0391 6704 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 715 3646 3678 3742 715
Size: 2931 32 64 3677
Fragments arranged by size:
3677 2931 64 32
Acc65I G'GTAC_C
Cuts at: 3720 3720
Size: 6704
AclI AA'CG_TT
Cuts at: 1503 3698 6544 1503
Size: 2195 2846 1663
Fragments arranged by size:
2846 2195 1663
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 926 1787 2287 2740 3178 3397 4529 4961
Size: 652 861 500 453 438 219 1132 432
Cuts at: 4961 5708 5976 274
Size: 747 268 1002
Fragments arranged by size:
1132 1002 861 747 652 500 453 438
432 268 219
AfeI AGC'GCT
Cuts at: 965 965
Size: 6704
AflII C'TTAA_G
Cuts at: 874 3418 874
Size: 2544 4160
AflIII A'CryG_T
Cuts at: 446 1250 4056 5160 6137 446
Size: 804 2806 1104 977 1013
Fragments arranged by size:
2806 1104 1013 977 804
AgeI A'CCGG_T
Cuts at: 5092 5092
Size: 6704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 937 1084 937
Size: 147 6557
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1467 1499 1467
Size: 32 6672
AlwNI CAG_nnn'CTG
Cuts at: 762 4009 5576 762
Size: 3247 1567 1890
Fragments arranged by size:
3247 1890 1567
ApaI G_GGCC'C
Cuts at: 790 790
Size: 6704
ApaLI G'TGCA_C
Cuts at: 5474 5474
Size: 6704
ApoI r'AATT_y
Cuts at: 198 502 855 1261 1688 1790 2053 2686 2788
Size: 304 353 406 427 102 263 633 102
Cuts at: 2788 2863 2901 2965 3395 3469 3616 3708 6057
Size: 75 38 64 430 74 147 92 2349
Cuts at: 6057 6313 198
Size: 256 589
Fragments arranged by size:
2349 633 589 430 427 406 353 304
263 256 147 102 102 92 75 74
64 38
AseI AT'TA_AT
Cuts at: 20 881 20
Size: 861 5843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6704
AvaI C'yCGr_G
Cuts at: 2507 2912 3724 2507
Size: 405 812 5487
Fragments arranged by size:
5487 812 405
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 656 689 656
Size: 33 6671
BamHI G'GATC_C
Cuts at: 608 3729 608
Size: 3121 3583
BanI G'GyrC_C
Cuts at: 2330 2540 3720 4134 4382 4417 2330
Size: 210 1180 414 248 35 4617
Fragments arranged by size:
4617 1180 414 248 210 35
BanII G_rGCy'C
Cuts at: 790 2919 3718 4748 790
Size: 2129 799 1030 2746
Fragments arranged by size:
2746 2129 1030 799
BbeI G_GCGC'C
Cuts at: 4138 4386 4138
Size: 248 6456
BbsI GAAGACnn'nnnn_
Cuts at: 497 1153 2254 3632 497
Size: 656 1101 1378 3569
Fragments arranged by size:
3569 1378 1101 656
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 6704
BciVI GTATCCnnnnn_n'
Cuts at: 4602 5363 6704 4602
Size: 761 1341 4602
Fragments arranged by size:
4602 1341 761
BclI T'GATC_A
Cuts at: 3315 4224 3315
Size: 909 5795
BfrBI ATG'CAT
Cuts at: 3579 3579
Size: 6704
BglII A'GATC_T
Cuts at: 1856 4219 6256 1856
Size: 2363 2037 2304
Fragments arranged by size:
2363 2304 2037
BlpI GC'TnA_GC
Cuts at: 2547 3816 2547
Size: 1269 5435
Bme1580I G_kGCm'C
Cuts at: 790 980 4329 4422 5478 790
Size: 190 3349 93 1056 2016
Fragments arranged by size:
3349 2016 1056 190 93
BmgBI CAC'GTC
Cuts at: 987 1242 987
Size: 255 6449
BmrI ACTGGGnnnn_n'
Cuts at: 3916 3997 4332 6476 3916
Size: 81 335 2144 4144
Fragments arranged by size:
4144 2144 335 81
BmtI G_CTAG'C
Cuts at: 2947 2947
Size: 6704
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5071 5071
Size: 6704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 2365 2426 2299
Size: 66 61 6577
Fragments arranged by size:
6577 66 61
Bpu10I CC'TnA_GC
Cuts at: 2207 2623 2207
Size: 416 6288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1020 1714 3295 3880 4759 5251 5549 1020
Size: 694 1581 585 879 492 298 2175
Fragments arranged by size:
2175 1581 879 694 585 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6408 6408
Size: 6704
BsaAI yAC'GTr
Cuts at: 1253 1615 4057 1253
Size: 362 2442 3900
Fragments arranged by size:
3900 2442 362
BsaBI GATnn'nnATC
Cuts at: 6 4218 6255 6
Size: 4212 2037 455
Fragments arranged by size:
4212 2037 455
BsaHI Gr'CG_yC
Cuts at: 4135 4383 5085 6581 4135
Size: 248 702 1496 4258
Fragments arranged by size:
4258 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3893 4414 5092 5366 5513 5924 3893
Size: 521 678 274 147 411 4673
Fragments arranged by size:
4673 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1321 1351 1321
Size: 30 6674
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 1342 2789 3416 206
Size: 1136 1447 627 3494
Fragments arranged by size:
3494 1447 1136 627
BseYI C'CCAG_C
Cuts at: 4129 4887 5464 5960 4129
Size: 758 577 496 4873
Fragments arranged by size:
4873 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 897 1606 3500 384
Size: 513 709 1894 3588
Fragments arranged by size:
3588 1894 709 513
BsiEI CG_ry'CG
Cuts at: 104 4246 4292 5500 104
Size: 4142 46 1208 1308
Fragments arranged by size:
4142 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2266 3718 4496 4686 5478 2266
Size: 1452 778 190 792 3492
Fragments arranged by size:
3492 1452 792 778 190
BsmI GAATG_Cn'
Cuts at: 1146 2924 3581 1146
Size: 1778 657 4269
Fragments arranged by size:
4269 1778 657
Bsp1286I G_dGCh'C
Cuts at: 790 980 2266 2919 3718 4329 4422 4496 4686
Size: 190 1286 653 799 611 93 74 190
Cuts at: 4686 4748 5478 790
Size: 62 730 2016
Fragments arranged by size:
2016 1286 799 730 653 611 190 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5924 5924
Size: 6704
BspHI T'CATG_A
Cuts at: 2744 5880 2744
Size: 3136 3568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1833 3138 3754 3968 4270 4651 1833
Size: 1305 616 214 302 381 3886
Fragments arranged by size:
3886 1305 616 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4996 5050 6534 6699 50
Size: 4946 54 1484 165 55
Fragments arranged by size:
4946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 257 4616 257
Size: 4359 2345
BsrFI r'CCGG_y
Cuts at: 4702 4883 5092 4702
Size: 181 209 6314
Fragments arranged by size:
6314 209 181
BsrGI T'GTAC_A
Cuts at: 3479 3479
Size: 6704
BssSI C'ACGA_G
Cuts at: 4975 5333 4975
Size: 358 6346
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2569 4081 2347
Size: 222 1512 4970
Fragments arranged by size:
4970 1512 222
BstBI TT'CG_AA
Cuts at: 5065 5065
Size: 6704
BstEII G'GTnAC_C
Cuts at: 3125 3125
Size: 6704
BstYI r'GATC_y
Cuts at: 1 357 608 1856 2487 3630 3729 4196 4219
Size: 356 251 1248 631 1143 99 467 23
Cuts at: 4219 4554 4800 5812 5898 5910 6256 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1248 1143 1012 631 467 449 356 346
335 251 246 99 86 23 12
BstZ17I GTA'TAC
Cuts at: 716 3647 716
Size: 2931 3773
BtgI C'CryG_G
Cuts at: 980 980
Size: 6704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4654 4835 4998 4654
Size: 181 163 6360
Fragments arranged by size:
6360 181 163
BtsI GCAGTG_nn'
Cuts at: 359 3185 3947 359
Size: 2826 762 3116
Fragments arranged by size:
3116 2826 762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3161 3196 3161
Size: 35 6669
DraI TTT'AAA
Cuts at: 2732 3703 2732
Size: 971 5733
DrdI GACnn_nn'nnGTC
Cuts at: 4410 5268 4410
Size: 858 5846
EaeI y'GGCC_r
Cuts at: 1962 3044 3077 4289 4463 4854 4881 6226 1962
Size: 1082 33 1212 174 391 27 1345 2440
Fragments arranged by size:
2440 1345 1212 1082 391 174 33 27
EagI C'GGCC_G
Cuts at: 4289 4289
Size: 6704
EarI CTCTTCn'nnn_
Cuts at: 945 1558 2099 2886 3140 3245 4727 4937 945
Size: 613 541 787 254 105 1482 210 2712
Fragments arranged by size:
2712 1482 787 613 541 254 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4912 5222 5368 6514 4912
Size: 310 146 1146 5102
Fragments arranged by size:
5102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3716 3716
Size: 6704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 926 1787 2287 2299 2365 2426 2740 3178
Size: 652 861 500 12 66 61 314 438
Cuts at: 3178 3397 4529 4961 5708 5976 274
Size: 219 1132 432 747 268 1002
Fragments arranged by size:
1132 1002 861 747 652 500 438 432
314 268 219 66 61 12
EcoO109I rG'GnC_Cy
Cuts at: 786 3111 3843 786
Size: 2325 732 3647
Fragments arranged by size:
3647 2325 732
EcoRI G'AATT_C
Cuts at: 502 2686 3708 502
Size: 2184 1022 3498
Fragments arranged by size:
3498 2184 1022
EcoRV GAT'ATC
Cuts at: 1784 1784
Size: 6704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1208 4723 1208
Size: 3515 3189
FspI TGC'GCA
Cuts at: 4485 4485
Size: 6704
HaeII r_GCGC'y
Cuts at: 756 967 4138 4386 5408 6387 756
Size: 211 3171 248 1022 979 1073
Fragments arranged by size:
3171 1073 1022 979 248 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 145 171 177 1321 1334 1353 1366 1567
Size: 6 26 6 1144 13 19 13 201
Cuts at: 1567 1599 2241 2273 3307 3339 3622 3654 4546
Size: 32 642 32 1034 32 283 32 892
Cuts at: 4546 4576 4578 4608 139
Size: 30 2 30 2235
Fragments arranged by size:
2235 1144 1034 892 642 283 201 32
32 32 32 30 30 26 19 13
13 6 6 2
HincII GTy'rAC
Cuts at: 2909 3588 3743 2909
Size: 679 155 5870
Fragments arranged by size:
5870 679 155
HpaI GTT'AAC
Cuts at: 2909 3588 2909
Size: 679 6025
KasI G'GCGC_C
Cuts at: 4134 4382 4134
Size: 248 6456
KpnI G_GTAC'C
Cuts at: 3724 3724
Size: 6704
MfeI C'AATT_G
Cuts at: 1913 1958 2960 1913
Size: 45 1002 5657
Fragments arranged by size:
5657 1002 45
MluI A'CGCG_T
Cuts at: 6137 6137
Size: 6704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1077 5375 5559 1077
Size: 4298 184 2222
Fragments arranged by size:
4298 2222 184
MscI TGG'CCA
Cuts at: 1964 3046 3079 4465 1964
Size: 1082 33 1386 4203
Fragments arranged by size:
4203 1386 1082 33
MslI CAynn'nnrTG
Cuts at: 269 4693 4820 269
Size: 4424 127 2153
Fragments arranged by size:
4424 2153 127
MspA1I CmG'CkG
Cuts at: 2269 2307 3815 4129 4489 5502 5747 2269
Size: 38 1508 314 360 1013 245 3226
Fragments arranged by size:
3226 1508 1013 360 314 245 38
NaeI GCC'GGC
Cuts at: 4885 4885
Size: 6704
NarI GG'CG_CC
Cuts at: 4135 4383 4135
Size: 248 6456
NgoMIV G'CCGG_C
Cuts at: 4883 4883
Size: 6704
NheI G'CTAG_C
Cuts at: 2943 2943
Size: 6704
NsiI A_TGCA'T
Cuts at: 3581 3581
Size: 6704
NspI r_CATG'y
Cuts at: 450 1812 2181 3757 4257 5164 450
Size: 1362 369 1576 500 907 1990
Fragments arranged by size:
1990 1576 1362 907 500 369
PciI A'CATG_T
Cuts at: 446 5160 446
Size: 4714 1990
PfoI T'CCnGG_A
Cuts at: 2381 2442 2381
Size: 61 6643
PmeI GTTT'AAAC
Cuts at: 3703 3703
Size: 6704
PmlI CAC'GTG
Cuts at: 1253 1253
Size: 6704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 239 271 5843 5875 239
Size: 32 5572 32 1068
Fragments arranged by size:
5572 1068 32 32
PpuMI rG'GwC_Cy
Cuts at: 3111 3111
Size: 6704
PsiI TTA'TAA
Cuts at: 2141 2141
Size: 6704
PspOMI G'GGCC_C
Cuts at: 786 786
Size: 6704
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 320 352 3224 3256 320
Size: 32 2872 32 3768
Fragments arranged by size:
3768 2872 32 32
PstI C_TGCA'G
Cuts at: 244 1830 1947 2316 2327 2681 3751 4436 244
Size: 1586 117 369 11 354 1070 685 2512
Fragments arranged by size:
2512 1586 1070 685 369 354 117 11
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6704
PvuII CAG'CTG
Cuts at: 2307 4129 4489 2307
Size: 1822 360 4522
Fragments arranged by size:
4522 1822 360
RsrII CG'GwC_CG
Cuts at: 4899 4899
Size: 6704
SacI G_AGCT'C
Cuts at: 3718 3718
Size: 6704
SalI G'TCGA_C
Cuts at: 3741 3741
Size: 6704
SapI GCTCTTCn'nnn_
Cuts at: 3140 4727 4937 3140
Size: 1587 210 4907
Fragments arranged by size:
4907 1587 210
SbfI CC_TGCA'GG
Cuts at: 1830 3751 1830
Size: 1921 4783
ScaI AGT'ACT
Cuts at: 6221 6221
Size: 6704
SfcI C'TryA_G
Cuts at: 33 240 1320 1826 1943 2312 2323 2677 3165
Size: 207 1080 506 117 369 11 354 488
Cuts at: 3165 3747 4432 5425 5616 33
Size: 582 685 993 191 1121
Fragments arranged by size:
1121 1080 993 685 582 506 488 369
354 207 191 117 11
SfoI GGC'GCC
Cuts at: 4136 4384 4136
Size: 248 6456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6704
SmaI CCC'GGG
Cuts at: 3726 3726
Size: 6704
SmlI C'TyrA_G
Cuts at: 874 999 1729 3274 3418 3859 4774 5266 5528
Size: 125 730 1545 144 441 915 492 262
Cuts at: 5528 874
Size: 2050
Fragments arranged by size:
2050 1545 915 730 492 441 262 144
125
SpeI A'CTAG_T
Cuts at: 67 669 67
Size: 602 6102
SphI G_CATG'C
Cuts at: 2181 3757 4257 2181
Size: 1576 500 4628
Fragments arranged by size:
4628 1576 500
SspI AAT'ATT
Cuts at: 531 1675 2515 531
Size: 1144 840 4720
Fragments arranged by size:
4720 1144 840
StyI C'CwwG_G
Cuts at: 3838 4192 3838
Size: 354 6350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 203 4287 4420 4879 5088 203
Size: 4084 133 459 209 1819
Fragments arranged by size:
4084 1819 459 209 133
TatI w'GTAC_w
Cuts at: 2412 3479 6219 2412
Size: 1067 2740 2897
Fragments arranged by size:
2897 2740 1067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2415 2447 3059 3091 6221 6253 2415
Size: 32 612 32 3130 32 2866
Fragments arranged by size:
3130 2866 612 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2253 4501 2253
Size: 2248 4456
XbaI T'CTAG_A
Cuts at: 1713 3735 1713
Size: 2022 4682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1795 4199 1795
Size: 2404 4300
XmaI C'CCGG_G
Cuts at: 3724 3724
Size: 6704
XmnI GAAnn'nnTTC
Cuts at: 3441 3441
Size: 6704
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AscI AvrII BcgI BglI
BsiWI BsmBI BssHII BstXI Bsu36I ClaI DraIII EcoNI
FseI FspAI HindIII NcoI NdeI NotI NruI PacI
PasI PflMI PshAI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI