Restriction map for
pF1KE0390
(Circular) (Six-base) MAPSORT of: pF1KE0390.seq Check: 2913 from: 1 to: 4577
pF1KE0390 4577 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 1334 1334
Size: 4577
AccI GT'mk_AC
Cuts at: 1472 1615 1472
Size: 143 4434
Acc65I G'GTAC_C
Cuts at: 1433 1593 1433
Size: 160 4417
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 2402 2834 3581 3849 958
Size: 1444 432 747 268 1686
Fragments arranged by size:
1686 1444 747 432 268
AflIII A'CryG_T
Cuts at: 770 1929 3033 4010 770
Size: 1159 1104 977 1337
Fragments arranged by size:
1337 1159 1104 977
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AhdI GACnn_n'nnGTC
Cuts at: 1335 1335
Size: 4577
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4577
AlwNI CAG_nnn'CTG
Cuts at: 341 504 936 1291 1882 3449 341
Size: 163 432 355 591 1567 1469
Fragments arranged by size:
1567 1469 591 432 355 163
ApaI G_GGCC'C
Cuts at: 594 1071 594
Size: 477 4100
ApaLI G'TGCA_C
Cuts at: 1147 3347 1147
Size: 2200 2377
ApoI r'AATT_y
Cuts at: 793 1581 3930 4186 793
Size: 788 2349 256 1184
Fragments arranged by size:
2349 1184 788 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 199 565 759 984 1057 1251 1507 1597 199
Size: 366 194 225 73 194 256 90 3179
Fragments arranged by size:
3179 366 256 225 194 194 90 73
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 193 1051 1433 1593 2007 2255 2290 193
Size: 858 382 160 414 248 35 2480
Fragments arranged by size:
2480 858 414 382 248 160 35
BanII G_rGCy'C
Cuts at: 410 594 862 1071 1591 2621 410
Size: 184 268 209 520 1030 2366
Fragments arranged by size:
2366 1030 520 268 209 184
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BbsI GAAGACnn'nnnn_
Cuts at: 1305 1305
Size: 4577
BbvCI CC'TCA_GC
Cuts at: 266 1112 266
Size: 846 3731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 965 999 965
Size: 34 4543
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 569 1462 569
Size: 893 3684
BglII A'GATC_T
Cuts at: 161 2092 4129 161
Size: 1931 2037 609
Fragments arranged by size:
2037 1931 609
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 233 348 416 594 1054 1071 1151 2202 2295
Size: 115 68 178 460 17 80 1051 93
Cuts at: 2295 3351 233
Size: 1056 1459
Fragments arranged by size:
1459 1056 1051 460 178 115 93 80
68 17
BmgBI CAC'GTC
Cuts at: 771 771
Size: 4577
BmrI ACTGGGnnnn_n'
Cuts at: 220 1789 1870 2205 4349 220
Size: 1569 81 335 2144 448
Fragments arranged by size:
2144 1569 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 2944 468
Size: 2476 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1150 1507 1515 697
Size: 453 357 8 3759
Fragments arranged by size:
3759 453 357 8
Bpu10I CC'TnA_GC
Cuts at: 266 1112 266
Size: 846 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1753 2632 3124 3422 1216
Size: 537 879 492 298 2371
Fragments arranged by size:
2371 879 537 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 624 4281 624
Size: 3657 920
BsaAI yAC'GTr
Cuts at: 426 1476 1930 426
Size: 1050 454 3073
Fragments arranged by size:
3073 1050 454
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 1331 1346 2008 2256 2958 4454 1331
Size: 15 662 248 702 1496 1454
Fragments arranged by size:
1496 1454 702 662 248 15
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 604 634 985 1015 1231 1261 604
Size: 30 351 30 216 30 3920
Fragments arranged by size:
3920 351 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 694 1147 1512 499
Size: 195 453 365 3564
Fragments arranged by size:
3564 453 365 195
BseYI C'CCAG_C
Cuts at: 397 550 813 855 2002 2760 3337 3833 397
Size: 153 263 42 1147 758 577 496 1141
Fragments arranged by size:
1147 1141 758 577 496 263 153 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1339 1531 955
Size: 384 192 4001
Fragments arranged by size:
4001 384 192
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 985 1151 1178 1481 1591 2369 2559 3351 985
Size: 166 27 303 110 778 190 792 2211
Fragments arranged by size:
2211 792 778 303 190 166 110 27
BsmBI CGTCTCn'nnnn_
Cuts at: 439 1402 439
Size: 963 3614
Bsp1286I G_dGCh'C
Cuts at: 233 348 410 416 594 862 985 1054 1071
Size: 115 62 6 178 268 123 69 17
Cuts at: 1071 1151 1178 1481 1591 2202 2295 2369 2559
Size: 80 27 303 110 611 93 74 190
Cuts at: 2559 2621 3351 233
Size: 62 730 1459
Fragments arranged by size:
1459 730 611 303 268 190 178 123
115 110 93 80 74 69 62 62
27 17 6
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 656 1043 1627 1841 2143 2524 656
Size: 387 584 214 302 381 2709
Fragments arranged by size:
2709 584 387 381 302 214
BsrBI CCG'CTC
Cuts at: 50 780 957 2869 2923 4407 4572 50
Size: 730 177 1912 54 1484 165 55
Fragments arranged by size:
1912 1484 730 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 168 1436 2575 2756 2965 168
Size: 1268 1139 181 209 1780
Fragments arranged by size:
1780 1268 1139 209 181
BsrGI T'GTAC_A
Cuts at: 1001 1001
Size: 4577
BssSI C'ACGA_G
Cuts at: 409 952 1177 1357 1405 2848 3206 409
Size: 543 225 180 48 1443 358 1780
Fragments arranged by size:
1780 1443 543 358 225 180 48
BstAPI GCAn_nnn'nTGC
Cuts at: 1425 1954 1425
Size: 529 4048
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstYI r'GATC_y
Cuts at: 1 161 926 1268 1602 2069 2092 2427 2673
Size: 160 765 342 334 467 23 335 246
Cuts at: 2673 3685 3771 3783 4129 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 765 467 449 346 342 335 334
246 160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 121 1292 121
Size: 1171 3406
BtgI C'CryG_G
Cuts at: 107 189 327 623 869 107
Size: 82 138 296 246 3815
Fragments arranged by size:
3815 296 246 138 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 983 1820 983
Size: 837 3740
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 978 978
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 166 428 781 1160 1298 1374 2162 2336 2727
Size: 262 353 379 138 76 788 174 391
Cuts at: 2727 2754 4099 166
Size: 27 1345 644
Fragments arranged by size:
1345 788 644 391 379 353 262 174
138 76 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 277 347 2600 2810 277
Size: 70 2253 210 2044
Fragments arranged by size:
2253 2044 210 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 313 1480 2785 3095 3241 4387 313
Size: 1167 1305 310 146 1146 503
Fragments arranged by size:
1305 1167 1146 503 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 958 1150 1507 1515 2402 2834 3581 3849
Size: 261 192 357 8 887 432 747 268
Cuts at: 3849 697
Size: 1425
Fragments arranged by size:
1425 887 747 432 357 268 261 192
8
EcoNI CCTnn'n_nnAGG
Cuts at: 352 652 919 352
Size: 300 267 4010
Fragments arranged by size:
4010 300 267
EcoO109I rG'GnC_Cy
Cuts at: 203 533 756 942 1068 1716 203
Size: 330 223 186 126 648 3064
Fragments arranged by size:
3064 648 330 223 186 126
EcoRI G'AATT_C
Cuts at: 793 1581 793
Size: 788 3789
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 1024 2358 1024
Size: 1334 3243
HaeII r_GCGC'y
Cuts at: 404 1291 2011 2259 3281 4260 404
Size: 887 720 248 1022 979 721
Fragments arranged by size:
1022 979 887 721 720 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1263 1321 1353 2419 2449 2451 2481 1231
Size: 32 58 32 1066 30 2 30 3327
Fragments arranged by size:
3327 1066 58 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1437 1597 1437
Size: 160 4417
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 466 3248 3432 466
Size: 2782 184 1611
Fragments arranged by size:
2782 1611 184
MscI TGG'CCA
Cuts at: 430 1376 2338 430
Size: 946 962 2669
Fragments arranged by size:
2669 962 946
MslI CAynn'nnrTG
Cuts at: 424 2566 2693 424
Size: 2142 127 2308
Fragments arranged by size:
2308 2142 127
MspA1I CmG'CkG
Cuts at: 329 504 631 1688 2002 2362 3375 3620 329
Size: 175 127 1057 314 360 1013 245 1286
Fragments arranged by size:
1286 1057 1013 360 314 245 175 127
NaeI GCC'GGC
Cuts at: 170 2758 170
Size: 2588 1989
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 189 623 107
Size: 82 434 4061
Fragments arranged by size:
4061 434 82
NgoMIV G'CCGG_C
Cuts at: 168 2756 168
Size: 2588 1989
NspI r_CATG'y
Cuts at: 747 1630 2130 3037 747
Size: 883 500 907 2287
Fragments arranged by size:
2287 907 883 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PmlI CAC'GTG
Cuts at: 426 426
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PpuMI rG'GwC_Cy
Cuts at: 203 203
Size: 4577
PspOMI G'GGCC_C
Cuts at: 590 1067 590
Size: 477 4100
PstI C_TGCA'G
Cuts at: 653 737 1040 1418 1624 2309 653
Size: 84 303 378 206 685 2921
Fragments arranged by size:
2921 685 378 303 206 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 504 631 2002 2362 504
Size: 127 1371 360 2719
Fragments arranged by size:
2719 1371 360 127
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SacII CC_GC'GG
Cuts at: 330 330
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SanDI GG'GwC_CC
Cuts at: 203 203
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 277 2600 2810 277
Size: 2323 210 2044
Fragments arranged by size:
2323 2044 210
SbfI CC_TGCA'GG
Cuts at: 1040 1624 1040
Size: 584 3993
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 649 733 1036 1414 1620 2305 3298 3489
Size: 616 84 303 378 206 685 993 191
Cuts at: 3489 33
Size: 1121
Fragments arranged by size:
1121 993 685 616 378 303 206 191
84
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 201 1599 201
Size: 1398 3179
SmlI C'TyrA_G
Cuts at: 1231 1732 2647 3139 3401 1231
Size: 501 915 492 262 2407
Fragments arranged by size:
2407 915 501 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 747 1630 2130 747
Size: 883 500 3194
Fragments arranged by size:
3194 883 500
StuI AGG'CCT
Cuts at: 848 1042 848
Size: 194 4383
StyI C'CwwG_G
Cuts at: 107 189 431 599 623 801 920 1711 2065
Size: 82 242 168 24 178 119 791 354
Cuts at: 2065 107
Size: 2619
Fragments arranged by size:
2619 791 354 242 178 168 119 82
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 534 805 2160 2293 2752 2961 436
Size: 98 271 1355 133 459 209 2052
Fragments arranged by size:
2052 1355 459 271 209 133 98
TatI w'GTAC_w
Cuts at: 1001 4092 1001
Size: 3091 1486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 569 601 801 833 835 867 4094 4126 569
Size: 32 200 32 2 32 3227 32 1020
Fragments arranged by size:
3227 1020 200 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 2072 423
Size: 1649 2928
XmaI C'CCGG_G
Cuts at: 199 1597 199
Size: 1398 3179
ZraI GAC'GTC
Cuts at: 1332 1332
Size: 4577
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI