Restriction map for
pF1KE0389
(Circular) (Six-base) MAPSORT of: pF1KE0389.seq Check: 6555 from: 1 to: 5621
pF1KE0389 5621 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 893 1430 893
Size: 537 5084
AccI GT'mk_AC
Cuts at: 1778 2659 1778
Size: 881 4740
Acc65I G'GTAC_C
Cuts at: 2637 2637
Size: 5621
AclI AA'CG_TT
Cuts at: 5461 5461
Size: 5621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 717 1530 1947 3446 3878 4625 4893 424
Size: 293 813 417 1499 432 747 268 1152
Fragments arranged by size:
1499 1152 813 747 432 417 293 268
AflIII A'CryG_T
Cuts at: 1321 2973 4077 5054 1321
Size: 1652 1104 977 1888
Fragments arranged by size:
1888 1652 1104 977
AgeI A'CCGG_T
Cuts at: 1367 4009 1367
Size: 2642 2979
AhdI GACnn_n'nnGTC
Cuts at: 405 405
Size: 5621
AleI CACnn'nnGTG
Cuts at: 1326 1825 1326
Size: 499 5122
AlwNI CAG_nnn'CTG
Cuts at: 1506 1584 1704 2015 2926 4493 1506
Size: 78 120 311 911 1567 2634
Fragments arranged by size:
2634 1567 911 311 120 78
ApaI G_GGCC'C
Cuts at: 2212 2457 2212
Size: 245 5376
ApaLI G'TGCA_C
Cuts at: 4391 4391
Size: 5621
ApoI r'AATT_y
Cuts at: 2625 4974 5230 2625
Size: 2349 256 3016
Fragments arranged by size:
3016 2349 256
AscI GG'CGCG_CC
Cuts at: 169 169
Size: 5621
AseI AT'TA_AT
Cuts at: 20 20
Size: 5621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
AvaI C'yCGr_G
Cuts at: 963 982 1605 1946 2158 2641 963
Size: 19 623 341 212 483 3943
Fragments arranged by size:
3943 623 483 341 212 19
BamHI G'GATC_C
Cuts at: 1079 2646 1079
Size: 1567 4054
BanI G'GyrC_C
Cuts at: 1401 1461 1491 2407 2637 3051 3299 3334 1401
Size: 60 30 916 230 414 248 35 3688
Fragments arranged by size:
3688 916 414 248 230 60 35 30
BanII G_rGCy'C
Cuts at: 854 970 2165 2212 2457 2600 2635 3665 854
Size: 116 1195 47 245 143 35 1030 2810
Fragments arranged by size:
2810 1195 1030 245 143 116 47 35
BbeI G_GCGC'C
Cuts at: 3055 3303 3055
Size: 248 5373
BbsI GAAGACnn'nnnn_
Cuts at: 2203 2203
Size: 5621
BbvCI CC'TCA_GC
Cuts at: 1952 1952
Size: 5621
BciVI GTATCCnnnnn_n'
Cuts at: 3519 4280 5621 3519
Size: 761 1341 3519
Fragments arranged by size:
3519 1341 761
BclI T'GATC_A
Cuts at: 1925 3141 1925
Size: 1216 4405
BglI GCCn_nnn'nGGC
Cuts at: 1610 1610
Size: 5621
BglII A'GATC_T
Cuts at: 1958 3136 5173 1958
Size: 1178 2037 2406
Fragments arranged by size:
2406 2037 1178
BlpI GC'TnA_GC
Cuts at: 801 1477 2733 801
Size: 676 1256 3689
Fragments arranged by size:
3689 1256 676
Bme1580I G_kGCm'C
Cuts at: 1406 1466 1850 2212 2410 2457 3246 3339 4395
Size: 60 384 362 198 47 789 93 1056
Cuts at: 4395 1406
Size: 2632
Fragments arranged by size:
2632 1056 789 384 362 198 93 60
47
BmgBI CAC'GTC
Cuts at: 860 1324 860
Size: 464 5157
BmrI ACTGGGnnnn_n'
Cuts at: 1400 1806 2833 2914 3249 5393 1400
Size: 406 1027 81 335 2144 1628
Fragments arranged by size:
2144 1628 1027 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3988 3988
Size: 5621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 1018 1381 1677 2433 344
Size: 674 363 296 756 3532
Fragments arranged by size:
3532 756 674 363 296
Bpu10I CC'TnA_GC
Cuts at: 1952 1952
Size: 5621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1173 2797 3676 4168 4466 1173
Size: 1624 879 492 298 2328
Fragments arranged by size:
2328 1624 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 135 1847 5325 135
Size: 1712 3478 431
Fragments arranged by size:
3478 1712 431
BsaAI yAC'GTr
Cuts at: 1143 2974 1143
Size: 1831 3790
BsaBI GATnn'nnATC
Cuts at: 6 3135 5172 6
Size: 3129 2037 455
Fragments arranged by size:
3129 2037 455
BsaHI Gr'CG_yC
Cuts at: 3052 3300 4002 5498 3052
Size: 248 702 1496 3175
Fragments arranged by size:
3175 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 2810 3331 4009 4283 4430 4841 1367
Size: 1443 521 678 274 147 411 2147
Fragments arranged by size:
2147 1443 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1282 1312 1579 1609 2123 2153 2281 2311 2515
Size: 30 267 30 514 30 128 30 204
Cuts at: 2515 2545 1282
Size: 30 4358
Fragments arranged by size:
4358 514 267 204 128 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 955 1969 2480 2494 955
Size: 1014 511 14 4082
Fragments arranged by size:
4082 1014 511 14
BseYI C'CCAG_C
Cuts at: 159 243 1573 1672 3046 3804 4381 4877 159
Size: 84 1330 99 1374 758 577 496 903
Fragments arranged by size:
1374 1330 903 758 577 496 99 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 1341 863
Size: 478 5143
BsiEI CG_ry'CG
Cuts at: 104 603 2398 3163 3209 4417 104
Size: 499 1795 765 46 1208 1308
Fragments arranged by size:
1795 1308 1208 765 499 46
BsiHKAI G_wGCw'C
Cuts at: 854 1985 2635 3413 3603 4395 854
Size: 1131 650 778 190 792 2080
Fragments arranged by size:
2080 1131 792 778 650 190
BsmI GAATG_Cn'
Cuts at: 1918 1918
Size: 5621
BsmBI CGTCTCn'nnnn_
Cuts at: 903 903
Size: 5621
Bsp1286I G_dGCh'C
Cuts at: 854 970 1406 1466 1850 1985 2165 2212 2410
Size: 116 436 60 384 135 180 47 198
Cuts at: 2410 2457 2600 2635 3246 3339 3413 3603 3665
Size: 47 143 35 611 93 74 190 62
Cuts at: 3665 4395 854
Size: 730 2080
Fragments arranged by size:
2080 730 611 436 384 198 190 180
143 135 116 93 74 62 60 47
47 35
BspEI T'CCGG_A
Cuts at: 4841 4841
Size: 5621
BspHI T'CATG_A
Cuts at: 854 1922 4797 854
Size: 1068 2875 1678
Fragments arranged by size:
2875 1678 1068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 893 1261 1430 2072 2671 2885 3187 3568 893
Size: 368 169 642 599 214 302 381 2946
Fragments arranged by size:
2946 642 599 381 368 302 214 169
BsrBI CCG'CTC
Cuts at: 50 749 945 1887 1892 2048 3913 3967 5451
Size: 699 196 942 5 156 1865 54 1484
Cuts at: 5451 5616 50
Size: 165 55
Fragments arranged by size:
1865 1484 942 699 196 165 156 55
54 5
BsrDI GCAATG_nn'
Cuts at: 651 737 3533 651
Size: 86 2796 2739
Fragments arranged by size:
2796 2739 86
BsrFI r'CCGG_y
Cuts at: 112 1367 2393 3619 3800 4009 112
Size: 1255 1026 1226 181 209 1724
Fragments arranged by size:
1724 1255 1226 1026 209 181
BssHII G'CGCG_C
Cuts at: 169 1914 169
Size: 1745 3876
BssSI C'ACGA_G
Cuts at: 212 1522 1555 2043 3892 4250 212
Size: 1310 33 488 1849 358 1583
Fragments arranged by size:
1849 1583 1310 488 358 33
BstAPI GCAn_nnn'nTGC
Cuts at: 2998 2998
Size: 5621
BstBI TT'CG_AA
Cuts at: 3982 3982
Size: 5621
BstEII G'GTnAC_C
Cuts at: 1650 1752 1650
Size: 102 5519
BstYI r'GATC_y
Cuts at: 1 383 1079 1625 1958 2646 3113 3136 3471
Size: 382 696 546 333 688 467 23 335
Cuts at: 3471 3717 4729 4815 4827 5173 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 696 688 546 467 449 382 346
335 333 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 544 842 1783 544
Size: 298 941 4382
Fragments arranged by size:
4382 941 298
BtgI C'CryG_G
Cuts at: 107 617 703 1189 2327 2411 2600 107
Size: 510 86 486 1138 84 189 3128
Fragments arranged by size:
3128 1138 510 486 189 86 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2103 3571 3752 3915 2103
Size: 1468 181 163 3809
Fragments arranged by size:
3809 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 139 2864 139
Size: 2725 2896
ClaI AT'CG_AT
Cuts at: 608 608
Size: 5621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2078 2113 2078
Size: 35 5586
DraI TTT'AAA
Cuts at: 2620 2620
Size: 5621
DrdI GACnn_nn'nnGTC
Cuts at: 2107 3327 4185 2107
Size: 1220 858 3543
Fragments arranged by size:
3543 1220 858
EaeI y'GGCC_r
Cuts at: 110 600 1559 2395 2471 3206 3380 3771 3798
Size: 490 959 836 76 735 174 391 27
Cuts at: 3798 5143 110
Size: 1345 588
Fragments arranged by size:
1345 959 836 735 588 490 391 174
76 27
EagI C'GGCC_G
Cuts at: 600 2395 3206 600
Size: 1795 811 3015
Fragments arranged by size:
3015 1795 811
EarI CTCTTCn'nnn_
Cuts at: 2074 3644 3854 2074
Size: 1570 210 3841
Fragments arranged by size:
3841 1570 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3829 4139 4285 5431 3829
Size: 310 146 1146 4019
Fragments arranged by size:
4019 1146 310 146
EcoICRI GAG'CTC
Cuts at: 852 2633 852
Size: 1781 3840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 424 717 1018 1381 1530 1677 1947 2433
Size: 80 293 301 363 149 147 270 486
Cuts at: 2433 3446 3878 4625 4893 344
Size: 1013 432 747 268 1072
Fragments arranged by size:
1072 1013 747 486 432 363 301 293
270 268 149 147 80
EcoNI CCTnn'n_nnAGG
Cuts at: 1972 1972
Size: 5621
EcoO109I rG'GnC_Cy
Cuts at: 248 1581 1787 1806 2208 2209 2453 2454 2760
Size: 1333 206 19 402 1 244 1 306
Cuts at: 2760 248
Size: 3109
Fragments arranged by size:
3109 1333 402 306 244 206 19 1
1
EcoRI G'AATT_C
Cuts at: 2625 2625
Size: 5621
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2013 3640 2013
Size: 1627 3994
FseI GG_CCGG'CC
Cuts at: 116 116
Size: 5621
FspI TGC'GCA
Cuts at: 3402 3402
Size: 5621
HaeII r_GCGC'y
Cuts at: 3055 3303 4325 5304 3055
Size: 248 1022 979 3372
Fragments arranged by size:
3372 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 391 423 1601 1633 2123 2155 2281
Size: 32 119 32 1178 32 490 32 126
Cuts at: 2281 2313 3463 3493 3495 3525 240
Size: 32 1150 30 2 30 2336
Fragments arranged by size:
2336 1178 1150 490 126 119 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1149 2022 2660 1149
Size: 873 638 4110
Fragments arranged by size:
4110 873 638
HindIII A'AGCT_T
Cuts at: 191 1995 191
Size: 1804 3817
KasI G'GCGC_C
Cuts at: 3051 3299 3051
Size: 248 5373
KpnI G_GTAC'C
Cuts at: 2641 2641
Size: 5621
MluI A'CGCG_T
Cuts at: 5054 5054
Size: 5621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 947 1134 1704 4292 4476 947
Size: 187 570 2588 184 2092
Fragments arranged by size:
2588 2092 570 187 184
MscI TGG'CCA
Cuts at: 1561 2473 3382 1561
Size: 912 909 3800
Fragments arranged by size:
3800 912 909
MslI CAynn'nnrTG
Cuts at: 1326 1648 1825 1843 2306 3610 3737 1326
Size: 322 177 18 463 1304 127 3210
Fragments arranged by size:
3210 1304 463 322 177 127 18
MspA1I CmG'CkG
Cuts at: 599 1941 2125 2215 2360 2732 3046 3406 4419
Size: 1342 184 90 145 372 314 360 1013
Cuts at: 4419 4664 599
Size: 245 1556
Fragments arranged by size:
1556 1342 1013 372 360 314 245 184
145 90
NaeI GCC'GGC
Cuts at: 114 3802 114
Size: 3688 1933
NarI GG'CG_CC
Cuts at: 3052 3300 3052
Size: 248 5373
NcoI C'CATG_G
Cuts at: 107 703 2327 2411 2600 107
Size: 596 1624 84 189 3128
Fragments arranged by size:
3128 1624 596 189 84
NdeI CA'TA_TG
Cuts at: 2443 2443
Size: 5621
NgoMIV G'CCGG_C
Cuts at: 112 3800 112
Size: 3688 1933
NspI r_CATG'y
Cuts at: 2674 3174 4081 2674
Size: 500 907 4214
Fragments arranged by size:
4214 907 500
PciI A'CATG_T
Cuts at: 4077 4077
Size: 5621
PflMI CCAn_nnn'nTGG
Cuts at: 1104 1104
Size: 5621
PfoI T'CCnGG_A
Cuts at: 658 1447 1831 1931 658
Size: 789 384 100 4348
Fragments arranged by size:
4348 789 384 100
PmeI GTTT'AAAC
Cuts at: 2620 2620
Size: 5621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 4760
Size: 32 5589
PpuMI rG'GwC_Cy
Cuts at: 248 1581 1787 248
Size: 1333 206 4082
Fragments arranged by size:
4082 1333 206
PspOMI G'GGCC_C
Cuts at: 2208 2453 2208
Size: 245 5376
PstI C_TGCA'G
Cuts at: 527 643 1094 1258 1511 2038 2375 2668 3353
Size: 116 451 164 253 527 337 293 685
Cuts at: 3353 527
Size: 2795
Fragments arranged by size:
2795 685 527 451 337 293 253 164
116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5621
PvuII CAG'CTG
Cuts at: 3046 3406 3046
Size: 360 5261
RsrII CG'GwC_CG
Cuts at: 556 3816 556
Size: 3260 2361
SacI G_AGCT'C
Cuts at: 854 2635 854
Size: 1781 3840
SalI G'TCGA_C
Cuts at: 2658 2658
Size: 5621
SanDI GG'GwC_CC
Cuts at: 248 248
Size: 5621
SapI GCTCTTCn'nnn_
Cuts at: 2074 3644 3854 2074
Size: 1570 210 3841
Fragments arranged by size:
3841 1570 210
SbfI CC_TGCA'GG
Cuts at: 2668 2668
Size: 5621
ScaI AGT'ACT
Cuts at: 5138 5138
Size: 5621
SexAI A'CCwGG_T
Cuts at: 1856 1856
Size: 5621
SfcI C'TryA_G
Cuts at: 33 523 639 1090 1254 1507 2034 2347 2371
Size: 490 116 451 164 253 527 313 24
Cuts at: 2371 2664 3349 4342 4533 33
Size: 293 685 993 191 1121
Fragments arranged by size:
1121 993 685 527 490 451 313 293
253 191 164 116 24
SfoI GGC'GCC
Cuts at: 3053 3301 3053
Size: 248 5373
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
SmaI CCC'GGG
Cuts at: 965 1607 1948 2643 965
Size: 642 341 695 3943
Fragments arranged by size:
3943 695 642 341
SmlI C'TyrA_G
Cuts at: 1152 2776 3691 4183 4445 1152
Size: 1624 915 492 262 2328
Fragments arranged by size:
2328 1624 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5621
SphI G_CATG'C
Cuts at: 2674 3174 2674
Size: 500 5121
SspI AAT'ATT
Cuts at: 396 396
Size: 5621
StuI AGG'CCT
Cuts at: 1690 1690
Size: 5621
StyI C'CwwG_G
Cuts at: 107 703 1466 2089 2327 2411 2600 2755 3109
Size: 596 763 623 238 84 189 155 354
Cuts at: 3109 107
Size: 2619
Fragments arranged by size:
2619 763 623 596 354 238 189 155
84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 544 2451 3204 3337 3796 4005 151
Size: 393 1907 753 133 459 209 1767
Fragments arranged by size:
1907 1767 753 459 393 209 133
TatI w'GTAC_w
Cuts at: 1586 5136 1586
Size: 3550 2071
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1282 1314 2559 2591 5138 5170 1282
Size: 32 1245 32 2547 32 1733
Fragments arranged by size:
2547 1733 1245 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 580 1710 3418 580
Size: 1130 1708 2783
Fragments arranged by size:
2783 1708 1130
XbaI T'CTAG_A
Cuts at: 1239 2652 1239
Size: 1413 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1857 2542 3116 1857
Size: 685 574 4362
Fragments arranged by size:
4362 685 574
XmaI C'CCGG_G
Cuts at: 963 1605 1946 2641 963
Size: 642 341 695 3943
Fragments arranged by size:
3943 695 642 341
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsrGI BstXI BstZ17I DraIII EcoRV
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI