Restriction map for
pF1KE0386
(Circular) (Six-base) MAPSORT of: pF1KE0386.seq Check: 1032 from: 1 to: 5195
pF1KE0386 5195 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1160 1160
Size: 5195
AccI GT'mk_AC
Cuts at: 491 773 2233 491
Size: 282 1460 3453
Fragments arranged by size:
3453 1460 282
Acc65I G'GTAC_C
Cuts at: 2184 2211 2184
Size: 27 5168
AclI AA'CG_TT
Cuts at: 5035 5035
Size: 5195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 1846 3020 3452 4199 4467 1792
Size: 54 1174 432 747 268 2520
Fragments arranged by size:
2520 1174 747 432 268 54
AflIII A'CryG_T
Cuts at: 2547 3651 4628 2547
Size: 1104 977 3114
Fragments arranged by size:
3114 1104 977
AgeI A'CCGG_T
Cuts at: 3583 3583
Size: 5195
AleI CACnn'nnGTG
Cuts at: 451 1000 451
Size: 549 4646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 1090 469
Size: 621 4574
AlwNI CAG_nnn'CTG
Cuts at: 979 1634 1716 2500 4067 979
Size: 655 82 784 1567 2107
Fragments arranged by size:
2107 1567 784 655 82
ApaI G_GGCC'C
Cuts at: 425 824 2012 425
Size: 399 1188 3608
Fragments arranged by size:
3608 1188 399
ApaLI G'TGCA_C
Cuts at: 2079 3965 2079
Size: 1886 3309
ApoI r'AATT_y
Cuts at: 910 2199 4548 4804 910
Size: 1289 2349 256 1301
Fragments arranged by size:
2349 1301 1289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5195
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
AvaI C'yCGr_G
Cuts at: 853 2215 853
Size: 1362 3833
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 783 816 783
Size: 33 5162
BamHI G'GATC_C
Cuts at: 1991 2220 1991
Size: 229 4966
BanI G'GyrC_C
Cuts at: 574 1044 2154 2184 2211 2625 2873 2908 574
Size: 470 1110 30 27 414 248 35 2861
Fragments arranged by size:
2861 1110 470 414 248 35 30 27
BanII G_rGCy'C
Cuts at: 425 824 1294 1520 2012 2066 2209 3239 425
Size: 399 470 226 492 54 143 1030 2381
Fragments arranged by size:
2381 1030 492 470 399 226 143 54
BbeI G_GCGC'C
Cuts at: 2629 2877 2629
Size: 248 4947
BbsI GAAGACnn'nnnn_
Cuts at: 1017 1834 1017
Size: 817 4378
BciVI GTATCCnnnnn_n'
Cuts at: 3093 3854 5195 3093
Size: 761 1341 3093
Fragments arranged by size:
3093 1341 761
BclI T'GATC_A
Cuts at: 2715 2715
Size: 5195
BglI GCCn_nnn'nGGC
Cuts at: 2007 2007
Size: 5195
BglII A'GATC_T
Cuts at: 2710 4747 2710
Size: 2037 3158
BlpI GC'TnA_GC
Cuts at: 2307 2307
Size: 5195
Bme1580I G_kGCm'C
Cuts at: 199 231 425 448 824 2012 2083 2159 2820
Size: 32 194 23 376 1188 71 76 661
Cuts at: 2820 2913 3969 199
Size: 93 1056 1425
Fragments arranged by size:
1425 1188 1056 661 376 194 93 76
71 32 23
BmrI ACTGGGnnnn_n'
Cuts at: 1622 2407 2488 2823 4967 1622
Size: 785 81 335 2144 1850
Fragments arranged by size:
2144 1850 785 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1501 1539 3562 1501
Size: 38 2023 3134
Fragments arranged by size:
3134 2023 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1072 1162 2080 780
Size: 292 90 918 3895
Fragments arranged by size:
3895 918 292 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 941 2371 3250 3742 4040 643
Size: 298 1430 879 492 298 1798
Fragments arranged by size:
1798 1430 879 492 298 298
BsaI GGTCTCn'nnnn_
Cuts at: 408 483 4899 408
Size: 75 4416 704
Fragments arranged by size:
4416 704 75
BsaAI yAC'GTr
Cuts at: 350 1548 1791 2548 350
Size: 1198 243 757 2997
Fragments arranged by size:
2997 1198 757 243
BsaBI GATnn'nnATC
Cuts at: 6 2709 4746 6
Size: 2703 2037 455
Fragments arranged by size:
2703 2037 455
BsaHI Gr'CG_yC
Cuts at: 317 2626 2874 3576 5072 317
Size: 2309 248 702 1496 440
Fragments arranged by size:
2309 1496 702 440 248
BsaWI w'CCGG_w
Cuts at: 2384 2905 3583 3857 4004 4415 2384
Size: 521 678 274 147 411 3164
Fragments arranged by size:
3164 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1194 1224 1194
Size: 30 5165
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 841 964 841
Size: 123 5072
BseYI C'CCAG_C
Cuts at: 376 562 703 816 1407 1441 1493 1836 1871
Size: 186 141 113 591 34 52 343 35
Cuts at: 1871 2620 3378 3955 4451 376
Size: 749 758 577 496 1120
Fragments arranged by size:
1120 758 749 591 577 496 343 186
141 113 52 35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1086 1086
Size: 5195
BsiEI CG_ry'CG
Cuts at: 104 2737 2783 3991 104
Size: 2633 46 1208 1308
Fragments arranged by size:
2633 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1520 2083 2209 2987 3177 3969 1520
Size: 563 126 778 190 792 2746
Fragments arranged by size:
2746 792 778 563 190 126
BsiWI C'GTAC_G
Cuts at: 350 350
Size: 5195
BsmI GAATG_Cn'
Cuts at: 895 1802 895
Size: 907 4288
Bsp1286I G_dGCh'C
Cuts at: 199 231 425 448 824 1294 1520 2012 2066
Size: 32 194 23 376 470 226 492 54
Cuts at: 2066 2083 2159 2209 2820 2913 2987 3177 3239
Size: 17 76 50 611 93 74 190 62
Cuts at: 3239 3969 199
Size: 730 1425
Fragments arranged by size:
1425 730 611 492 470 376 226 194
190 93 76 74 62 54 50 32
23 17
BspEI T'CCGG_A
Cuts at: 4415 4415
Size: 5195
BspHI T'CATG_A
Cuts at: 4371 4371
Size: 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 1488 2035 2245 2459 2761 3142 1160
Size: 328 547 210 214 302 381 3213
Fragments arranged by size:
3213 547 381 328 302 214 210
BsrBI CCG'CTC
Cuts at: 50 3487 3541 5025 5190 50
Size: 3437 54 1484 165 55
Fragments arranged by size:
3437 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 510 3107 510
Size: 2597 2598
BsrFI r'CCGG_y
Cuts at: 1509 3193 3374 3583 1509
Size: 1684 181 209 3121
Fragments arranged by size:
3121 1684 209 181
BsrGI T'GTAC_A
Cuts at: 986 986
Size: 5195
BssSI C'ACGA_G
Cuts at: 3466 3824 3466
Size: 358 4837
BstAPI GCAn_nnn'nTGC
Cuts at: 2572 2572
Size: 5195
BstBI TT'CG_AA
Cuts at: 3556 3556
Size: 5195
BstXI CCAn_nnnn'nTGG
Cuts at: 265 1164 265
Size: 899 4296
BstYI r'GATC_y
Cuts at: 1 213 551 1991 2220 2687 2710 3045 3291
Size: 212 338 1440 229 467 23 335 246
Cuts at: 3291 4303 4389 4401 4747 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1440 1012 467 449 346 338 335 246
229 212 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1247 1247
Size: 5195
BtgI C'CryG_G
Cuts at: 107 258 386 440 107
Size: 151 128 54 4862
Fragments arranged by size:
4862 151 128 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 356 3145 3326 3489 356
Size: 2789 181 163 2062
Fragments arranged by size:
2789 2062 181 163
BtsI GCAGTG_nn'
Cuts at: 2438 2438
Size: 5195
ClaI AT'CG_AT
Cuts at: 281 281
Size: 5195
DraI TTT'AAA
Cuts at: 2194 2194
Size: 5195
DrdI GACnn_nn'nnGTC
Cuts at: 172 2901 3759 172
Size: 2729 858 1608
Fragments arranged by size:
2729 1608 858
EaeI y'GGCC_r
Cuts at: 287 1337 1542 1619 2780 2954 3345 3372 4717
Size: 1050 205 77 1161 174 391 27 1345
Cuts at: 4717 287
Size: 765
Fragments arranged by size:
1345 1161 1050 765 391 205 174 77
27
EagI C'GGCC_G
Cuts at: 2780 2780
Size: 5195
EarI CTCTTCn'nnn_
Cuts at: 746 1114 1688 3218 3428 746
Size: 368 574 1530 210 2513
Fragments arranged by size:
2513 1530 574 368 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 3403 3713 3859 5005 2137
Size: 1266 310 146 1146 2327
Fragments arranged by size:
2327 1266 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1518 2207 1518
Size: 689 4506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1072 1162 1792 1846 2080 3020 3452 4199
Size: 292 90 630 54 234 940 432 747
Cuts at: 4199 4467 780
Size: 268 1508
Fragments arranged by size:
1508 940 747 630 432 292 268 234
90 54
EcoNI CCTnn'n_nnAGG
Cuts at: 946 1935 2113 946
Size: 989 178 4028
Fragments arranged by size:
4028 989 178
EcoO109I rG'GnC_Cy
Cuts at: 559 700 820 1152 1928 2009 2024 2334 559
Size: 141 120 332 776 81 15 310 3420
Fragments arranged by size:
3420 776 332 310 141 120 81 15
EcoRI G'AATT_C
Cuts at: 910 2199 910
Size: 1289 3906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1947 2100 3214 1947
Size: 153 1114 3928
Fragments arranged by size:
3928 1114 153
FspI TGC'GCA
Cuts at: 2976 2976
Size: 5195
HaeII r_GCGC'y
Cuts at: 1510 2629 2877 3899 4878 1510
Size: 1119 248 1022 979 1827
Fragments arranged by size:
1827 1119 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 543 575 3037 3067 3069 3099 388
Size: 32 123 32 2462 30 2 30 2484
Fragments arranged by size:
2484 2462 123 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2234 2234
Size: 5195
KasI G'GCGC_C
Cuts at: 2625 2873 2625
Size: 248 4947
KpnI G_GTAC'C
Cuts at: 2188 2215 2188
Size: 27 5168
MluI A'CGCG_T
Cuts at: 4628 4628
Size: 5195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2100 3866 4050 2100
Size: 1766 184 3245
Fragments arranged by size:
3245 1766 184
MscI TGG'CCA
Cuts at: 289 1339 1544 1621 2956 289
Size: 1050 205 77 1335 2528
Fragments arranged by size:
2528 1335 1050 205 77
MslI CAynn'nnrTG
Cuts at: 451 1000 1550 3184 3311 451
Size: 549 550 1634 127 2335
Fragments arranged by size:
2335 1634 550 549 127
MspA1I CmG'CkG
Cuts at: 221 451 566 707 1080 1407 1875 2306 2620
Size: 230 115 141 373 327 468 431 314
Cuts at: 2620 2980 3993 4238 221
Size: 360 1013 245 1178
Fragments arranged by size:
1178 1013 468 431 373 360 327 314
245 230 141 115
NaeI GCC'GGC
Cuts at: 3376 3376
Size: 5195
NarI GG'CG_CC
Cuts at: 2626 2874 2626
Size: 248 4947
NcoI C'CATG_G
Cuts at: 107 258 107
Size: 151 5044
NgoMIV G'CCGG_C
Cuts at: 3374 3374
Size: 5195
NspI r_CATG'y
Cuts at: 2248 2748 3655 2248
Size: 500 907 3788
Fragments arranged by size:
3788 907 500
PasI CC'CwG_GG
Cuts at: 2067 2067
Size: 5195
PciI A'CATG_T
Cuts at: 3651 3651
Size: 5195
PflMI CCAn_nnn'nTGG
Cuts at: 326 329 569 1335 1755 326
Size: 3 240 766 420 3766
Fragments arranged by size:
3766 766 420 240 3
PfoI T'CCnGG_A
Cuts at: 1178 1313 1178
Size: 135 5060
PmeI GTTT'AAAC
Cuts at: 2194 2194
Size: 5195
PmlI CAC'GTG
Cuts at: 1548 1548
Size: 5195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 4334
Size: 32 5163
PpuMI rG'GwC_Cy
Cuts at: 559 1152 1928 2024 559
Size: 593 776 96 3730
Fragments arranged by size:
3730 776 593 96
PshAI GACnn'nnGTC
Cuts at: 2116 2116
Size: 5195
PspOMI G'GGCC_C
Cuts at: 421 820 2008 421
Size: 399 1188 3608
Fragments arranged by size:
3608 1188 399
PstI C_TGCA'G
Cuts at: 1079 1085 2242 2927 1079
Size: 6 1157 685 3347
Fragments arranged by size:
3347 1157 685 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5195
PvuII CAG'CTG
Cuts at: 451 707 1080 1875 2620 2980 451
Size: 256 373 795 745 360 2666
Fragments arranged by size:
2666 795 745 373 360 256
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 5195
SacI G_AGCT'C
Cuts at: 1520 2209 1520
Size: 689 4506
SalI G'TCGA_C
Cuts at: 2232 2232
Size: 5195
SanDI GG'GwC_CC
Cuts at: 559 559
Size: 5195
SapI GCTCTTCn'nnn_
Cuts at: 1114 1688 3218 3428 1114
Size: 574 1530 210 2881
Fragments arranged by size:
2881 1530 574 210
SbfI CC_TGCA'GG
Cuts at: 2242 2242
Size: 5195
ScaI AGT'ACT
Cuts at: 1282 4712 1282
Size: 3430 1765
SfcI C'TryA_G
Cuts at: 33 890 1075 1081 2238 2923 3916 4107 33
Size: 857 185 6 1157 685 993 191 1121
Fragments arranged by size:
1157 1121 993 857 685 191 185 6
SfoI GGC'GCC
Cuts at: 2627 2875 2627
Size: 248 4947
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
SgrAI Cr'CCGG_yG
Cuts at: 1509 1509
Size: 5195
SmaI CCC'GGG
Cuts at: 2217 2217
Size: 5195
SmlI C'TyrA_G
Cuts at: 622 956 2350 3265 3757 4019 622
Size: 334 1394 915 492 262 1798
Fragments arranged by size:
1798 1394 915 492 334 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5195
SphI G_CATG'C
Cuts at: 2248 2748 2248
Size: 500 4695
StuI AGG'CCT
Cuts at: 920 920
Size: 5195
StyI C'CwwG_G
Cuts at: 107 258 696 746 842 1389 2329 2683 107
Size: 151 438 50 96 547 940 354 2619
Fragments arranged by size:
2619 940 547 438 354 151 96 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 396 1219 2778 2911 3370 3579 396
Size: 823 1559 133 459 209 2012
Fragments arranged by size:
2012 1559 823 459 209 133
TatI w'GTAC_w
Cuts at: 986 1280 4710 986
Size: 294 3430 1471
Fragments arranged by size:
3430 1471 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1194 1226 4712 4744 1194
Size: 32 3486 32 1645
Fragments arranged by size:
3486 1645 32 32
Tth111I GACn'n_nGTC
Cuts at: 1224 2992 1224
Size: 1768 3427
XbaI T'CTAG_A
Cuts at: 483 2226 483
Size: 1743 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 384 2690 114
Size: 270 2306 2619
Fragments arranged by size:
2619 2306 270
XmaI C'CCGG_G
Cuts at: 2215 2215
Size: 5195
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsmBI BssHII BstEII
BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
PspXI PsrI SacII SexAI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI