Restriction map for
pF1KE0384
(Circular) (Six-base) MAPSORT of: pF1KE0384.seq Check: 5617 from: 1 to: 4580
pF1KE0384 4580 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 893 893
Size: 4580
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2405 2837 3584 3852 1216
Size: 1189 432 747 268 1944
Fragments arranged by size:
1944 1189 747 432 268
AflIII A'CryG_T
Cuts at: 986 1932 3036 4013 986
Size: 946 1104 977 1553
Fragments arranged by size:
1553 1104 977 946
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4580
AlwNI CAG_nnn'CTG
Cuts at: 748 801 813 1449 1885 3452 748
Size: 53 12 636 436 1567 1876
Fragments arranged by size:
1876 1567 636 436 53 12
ApaI G_GGCC'C
Cuts at: 238 287 341 396 596 623 238
Size: 49 54 55 200 27 4195
Fragments arranged by size:
4195 200 55 54 49 27
ApaLI G'TGCA_C
Cuts at: 360 3350 360
Size: 2990 1590
ApoI r'AATT_y
Cuts at: 1584 3933 4189 1584
Size: 2349 256 1975
Fragments arranged by size:
2349 1975 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 213 231 298 334 1015 1102 1600 213
Size: 18 67 36 681 87 498 3193
Fragments arranged by size:
3193 681 498 87 67 36 18
BamHI G'GATC_C
Cuts at: 1328 1605 1328
Size: 277 4303
BanI G'GyrC_C
Cuts at: 124 190 217 303 586 1066 1078 1211 1307
Size: 66 27 86 283 480 12 133 96
Cuts at: 1307 1596 2010 2258 2293 124
Size: 289 414 248 35 2411
Fragments arranged by size:
2411 480 414 289 283 248 133 96
86 66 35 27 12
BanII G_rGCy'C
Cuts at: 238 287 341 396 536 596 623 1022 1148
Size: 49 54 55 140 60 27 399 126
Cuts at: 1148 1285 1594 2624 238
Size: 137 309 1030 2194
Fragments arranged by size:
2194 1030 399 309 140 137 126 60
55 54 49 27
BbeI G_GCGC'C
Cuts at: 194 221 590 2014 2262 194
Size: 27 369 1424 248 2512
Fragments arranged by size:
2512 1424 369 248 27
BbsI GAAGACnn'nnnn_
Cuts at: 1508 1508
Size: 4580
BbvCI CC'TCA_GC
Cuts at: 841 930 841
Size: 89 4491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4546
BciVI GTATCCnnnnn_n'
Cuts at: 1347 2478 3239 4580 1347
Size: 1131 761 1341 1347
Fragments arranged by size:
1347 1341 1131 761
BclI T'GATC_A
Cuts at: 1160 2100 1160
Size: 940 3640
BglI GCCn_nnn'nGGC
Cuts at: 112 169 336 584 1384 112
Size: 57 167 248 800 3308
Fragments arranged by size:
3308 800 248 167 57
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 911 1692 911
Size: 781 3799
Bme1580I G_kGCm'C
Cuts at: 155 238 287 306 341 364 396 596 623
Size: 83 49 19 35 23 32 200 27
Cuts at: 623 758 1081 1103 2205 2298 3354 155
Size: 135 323 22 1102 93 1056 1381
Fragments arranged by size:
1381 1102 1056 323 200 135 93 83
49 35 32 27 23 22 19
BmrI ACTGGGnnnn_n'
Cuts at: 736 1401 1792 1873 2208 4352 736
Size: 665 391 81 335 2144 964
Fragments arranged by size:
2144 964 665 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 667 673 715 808 841 929 937 565
Size: 102 6 42 93 33 88 8 4208
Fragments arranged by size:
4208 102 93 88 42 33 8 6
Bpu10I CC'TnA_GC
Cuts at: 250 841 930 1189 250
Size: 591 89 259 3641
Fragments arranged by size:
3641 591 259 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4284 4284
Size: 4580
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 964 1333 2094 4131 6
Size: 958 369 761 2037 455
Fragments arranged by size:
2037 958 761 455 369
BsaHI Gr'CG_yC
Cuts at: 191 218 587 659 890 2011 2259 2961 4457
Size: 27 369 72 231 1121 248 702 1496
Cuts at: 4457 191
Size: 314
Fragments arranged by size:
1496 1121 702 369 314 248 231 72
27
BsaWI w'CCGG_w
Cuts at: 1285 1769 2290 2968 3242 3389 3800 1285
Size: 484 521 678 274 147 411 2065
Fragments arranged by size:
2065 678 521 484 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 910 940 910
Size: 30 4550
BseYI C'CCAG_C
Cuts at: 395 546 735 1178 1479 2005 2763 3340 3836
Size: 151 189 443 301 526 758 577 496
Cuts at: 3836 395
Size: 1139
Fragments arranged by size:
1139 758 577 526 496 443 301 189
151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1449 244
Size: 1205 3375
BsiEI CG_ry'CG
Cuts at: 104 209 464 690 2122 2168 3376 104
Size: 105 255 226 1432 46 1208 1308
Fragments arranged by size:
1432 1308 1208 255 226 105 46
BsiHKAI G_wGCw'C
Cuts at: 364 460 536 923 1022 1594 2372 2562 3354
Size: 96 76 387 99 572 778 190 792
Cuts at: 3354 364
Size: 1590
Fragments arranged by size:
1590 792 778 572 387 190 99 96
76
BsmI GAATG_Cn'
Cuts at: 1027 1027
Size: 4580
Bsp1286I G_dGCh'C
Cuts at: 155 238 287 306 341 364 396 460 536
Size: 83 49 19 35 23 32 64 76
Cuts at: 536 596 623 758 923 1022 1081 1103 1148
Size: 60 27 135 165 99 59 22 45
Cuts at: 1148 1285 1594 2205 2298 2372 2562 2624 3354
Size: 137 309 611 93 74 190 62 730
Cuts at: 3354 155
Size: 1381
Fragments arranged by size:
1381 730 611 309 190 165 137 135
99 93 83 76 74 64 62 60
59 49 45 35 32 27 23 22
19
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 3756 3756
Size: 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1630 1844 2146 2527 1630
Size: 214 302 381 3683
Fragments arranged by size:
3683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 563 2872 2926 4410 4575 50
Size: 513 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 513 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1454 2492 1400
Size: 54 1038 3488
Fragments arranged by size:
3488 1038 54
BsrFI r'CCGG_y
Cuts at: 538 760 857 1069 1367 2578 2759 2968 538
Size: 222 97 212 298 1211 181 209 2150
Fragments arranged by size:
2150 1211 298 222 212 209 181 97
BssHII G'CGCG_C
Cuts at: 254 317 499 666 254
Size: 63 182 167 4168
Fragments arranged by size:
4168 182 167 63
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 327 1957 327
Size: 1630 2950
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstYI r'GATC_y
Cuts at: 1 1328 1605 2072 2095 2430 2676 3688 3774
Size: 1327 277 467 23 335 246 1012 86
Cuts at: 3774 3786 4132 1
Size: 12 346 449
Fragments arranged by size:
1327 1012 467 449 346 335 277 246
86 23 12
BtgI C'CryG_G
Cuts at: 308 608 1148 308
Size: 300 540 3740
Fragments arranged by size:
3740 540 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 126 1209 2530 2711 2874 126
Size: 1083 1321 181 163 1832
Fragments arranged by size:
1832 1321 1083 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1474 1823 1466
Size: 8 349 4223
Fragments arranged by size:
4223 349 8
DraI TTT'AAA
Cuts at: 1579 1579
Size: 4580
DrdI GACnn_nn'nnGTC
Cuts at: 2286 3144 2286
Size: 858 3722
EaeI y'GGCC_r
Cuts at: 161 311 461 611 1071 1365 2165 2339 2730
Size: 150 150 150 460 294 800 174 391
Cuts at: 2730 2757 4102 161
Size: 27 1345 639
Fragments arranged by size:
1345 800 639 460 391 294 174 150
150 150 27
EagI C'GGCC_G
Cuts at: 461 2165 461
Size: 1704 2876
EarI CTCTTCn'nnn_
Cuts at: 431 2603 2813 431
Size: 2172 210 2198
Fragments arranged by size:
2198 2172 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 534 1020 1592 534
Size: 486 572 3522
Fragments arranged by size:
3522 572 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 667 673 715 808 841 929 937 1216
Size: 102 6 42 93 33 88 8 279
Cuts at: 1216 2405 2837 3584 3852 565
Size: 1189 432 747 268 1293
Fragments arranged by size:
1293 1189 747 432 279 268 102 93
88 42 33 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 697 697
Size: 4580
EcoO109I rG'GnC_Cy
Cuts at: 284 338 392 593 620 926 1446 1719 284
Size: 54 54 201 27 306 520 273 3145
Fragments arranged by size:
3145 520 306 273 201 54 54 27
EcoRI G'AATT_C
Cuts at: 1584 1584
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1560 2599 1560
Size: 1039 3541
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 181 194 221 419 487 590 2014 2262 3284
Size: 13 27 198 68 103 1424 248 1022
Cuts at: 3284 4263 181
Size: 979 498
Fragments arranged by size:
1424 1022 979 498 248 198 103 68
27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 910 942 2422 2452 2454 2484 910
Size: 32 1480 30 2 30 3006
Fragments arranged by size:
3006 1480 32 30 30 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
KasI G'GCGC_C
Cuts at: 190 217 586 2010 2258 190
Size: 27 369 1424 248 2512
Fragments arranged by size:
2512 1424 369 248 27
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 866 3251 3435 866
Size: 2385 184 2011
Fragments arranged by size:
2385 2011 184
MscI TGG'CCA
Cuts at: 2341 2341
Size: 4580
MslI CAynn'nnrTG
Cuts at: 2569 2696 2569
Size: 127 4453
MspA1I CmG'CkG
Cuts at: 243 310 327 369 471 554 730 786 798
Size: 67 17 42 102 83 176 56 12
Cuts at: 798 1064 1182 1691 2005 2365 3378 3623 243
Size: 266 118 509 314 360 1013 245 1200
Fragments arranged by size:
1200 1013 509 360 314 266 245 176
118 102 83 67 56 42 17 12
NaeI GCC'GGC
Cuts at: 859 2761 859
Size: 1902 2678
NarI GG'CG_CC
Cuts at: 191 218 587 2011 2259 191
Size: 27 369 1424 248 2512
Fragments arranged by size:
2512 1424 369 248 27
NcoI C'CATG_G
Cuts at: 608 1148 608
Size: 540 4040
NgoMIV G'CCGG_C
Cuts at: 857 2759 857
Size: 1902 2678
NspI r_CATG'y
Cuts at: 990 1044 1633 2133 3040 990
Size: 54 589 500 907 2530
Fragments arranged by size:
2530 907 589 500 54
PasI CC'CwG_GG
Cuts at: 623 1359 1496 623
Size: 736 137 3707
Fragments arranged by size:
3707 736 137
PciI A'CATG_T
Cuts at: 986 3036 986
Size: 2050 2530
PflMI CCAn_nnn'nTGG
Cuts at: 813 1449 813
Size: 636 3944
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PpuMI rG'GwC_Cy
Cuts at: 926 1446 926
Size: 520 4060
PspOMI G'GGCC_C
Cuts at: 234 283 337 392 592 619 234
Size: 49 54 55 200 27 4195
Fragments arranged by size:
4195 200 55 54 49 27
PstI C_TGCA'G
Cuts at: 455 491 899 1280 1627 2312 455
Size: 36 408 381 347 685 2723
Fragments arranged by size:
2723 685 408 381 347 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 471 730 786 798 1182 2005 2365 471
Size: 259 56 12 384 823 360 2686
Fragments arranged by size:
2686 823 384 360 259 56 12
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 536 1022 1594 536
Size: 486 572 3522
Fragments arranged by size:
3522 572 486
SacII CC_GC'GG
Cuts at: 311 311
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 431 2603 2813 431
Size: 2172 210 2198
Fragments arranged by size:
2198 2172 210
SbfI CC_TGCA'GG
Cuts at: 899 1627 899
Size: 728 3852
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 451 487 895 936 1276 1623 2308 3301
Size: 418 36 408 41 340 347 685 993
Cuts at: 3301 3492 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 418 408 347 340 191
41 36
SfoI GGC'GCC
Cuts at: 192 219 588 2012 2260 192
Size: 27 369 1424 248 2512
Fragments arranged by size:
2512 1424 369 248 27
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 233 300 336 1104 1602 233
Size: 67 36 768 498 3211
Fragments arranged by size:
3211 768 498 67 36
SmlI C'TyrA_G
Cuts at: 1735 2650 3142 3404 1735
Size: 915 492 262 2911
Fragments arranged by size:
2911 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
SrfI GCCC'GGGC
Cuts at: 336 1104 336
Size: 768 3812
StuI AGG'CCT
Cuts at: 1387 1519 1387
Size: 132 4448
StyI C'CwwG_G
Cuts at: 608 1148 1568 1714 2068 608
Size: 540 420 146 354 3120
Fragments arranged by size:
3120 540 420 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1351 1396 2163 2296 2755 2964 195
Size: 1156 45 767 133 459 209 1811
Fragments arranged by size:
1811 1156 767 459 209 133 45
TatI w'GTAC_w
Cuts at: 1122 4095 1122
Size: 2973 1607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 4548
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 2075 153
Size: 1922 2658
XmaI C'CCGG_G
Cuts at: 231 298 334 1102 1600 231
Size: 67 36 768 498 3211
Fragments arranged by size:
3211 768 498 67 36
ZraI GAC'GTC
Cuts at: 891 891
Size: 4580
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI