Restriction map for
pF1KE0377
(Circular) (Six-base) MAPSORT of: pF1KE0377.seq Check: 4059 from: 1 to: 4595
pF1KE0377 4595 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 803 803
Size: 4595
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 2420 2852 3599 3867 1295
Size: 1125 432 747 268 2023
Fragments arranged by size:
2023 1125 747 432 268
AfeI AGC'GCT
Cuts at: 298 298
Size: 4595
AflIII A'CryG_T
Cuts at: 1523 1536 1947 3051 4028 1523
Size: 13 411 1104 977 2090
Fragments arranged by size:
2090 1104 977 411 13
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AleI CACnn'nnGTG
Cuts at: 1276 1276
Size: 4595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1051 1051
Size: 4595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 682
Size: 32 4563
AlwNI CAG_nnn'CTG
Cuts at: 1900 3467 1900
Size: 1567 3028
ApaI G_GGCC'C
Cuts at: 332 332
Size: 4595
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 1599 3948 4204 1599
Size: 2349 256 1990
Fragments arranged by size:
2349 1990 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 1331 1615 1331
Size: 284 4311
BamHI G'GATC_C
Cuts at: 885 1620 885
Size: 735 3860
BanI G'GyrC_C
Cuts at: 601 1611 2025 2273 2308 601
Size: 1010 414 248 35 2888
Fragments arranged by size:
2888 1010 414 248 35
BanII G_rGCy'C
Cuts at: 332 1297 1454 1609 2639 332
Size: 965 157 155 1030 2288
Fragments arranged by size:
2288 1030 965 157 155
BbeI G_GCGC'C
Cuts at: 605 2029 2277 605
Size: 1424 248 2923
Fragments arranged by size:
2923 1424 248
BbvCI CC'TCA_GC
Cuts at: 392 392
Size: 4595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1468 1502 90
Size: 34 1344 34 3183
Fragments arranged by size:
3183 1344 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3254 4595 2493
Size: 761 1341 2493
Fragments arranged by size:
2493 1341 761
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4595
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 332 595 1015 1273 1400 2220 2313 3369 332
Size: 263 420 258 127 820 93 1056 1558
Fragments arranged by size:
1558 1056 820 420 263 258 127 93
BmgBI CAC'GTC
Cuts at: 1524 1524
Size: 4595
BmrI ACTGGGnnnn_n'
Cuts at: 1807 1888 2223 4367 1807
Size: 81 335 2144 2035
Fragments arranged by size:
2144 2035 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 792 1219 2962 792
Size: 427 1743 2425
Fragments arranged by size:
2425 1743 427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 859
Size: 4595
Bpu10I CC'TnA_GC
Cuts at: 392 628 392
Size: 236 4359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1771 2650 3142 3440 1771
Size: 879 492 298 2926
Fragments arranged by size:
2926 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1012 1301 4299 1012
Size: 289 2998 1308
Fragments arranged by size:
2998 1308 289
BsaAI yAC'GTr
Cuts at: 1539 1948 1539
Size: 409 4186
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 581 602 2026 2274 2976 4472 581
Size: 21 1424 248 702 1496 704
Fragments arranged by size:
1496 1424 704 702 248 21
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 574 952 955 1108 409
Size: 165 378 3 153 3896
Fragments arranged by size:
3896 378 165 153 3
BseYI C'CCAG_C
Cuts at: 723 1290 1346 2020 2778 3355 3851 723
Size: 567 56 674 758 577 496 1467
Fragments arranged by size:
1467 758 674 577 567 496 56
BsiEI CG_ry'CG
Cuts at: 104 2137 2183 3391 104
Size: 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 869 1030 1454 1609 2387 2577 3369 869
Size: 161 424 155 778 190 792 2095
Fragments arranged by size:
2095 792 778 424 190 161 155
Bsp1286I G_dGCh'C
Cuts at: 332 595 869 1015 1030 1273 1297 1400 1454
Size: 263 274 146 15 243 24 103 54
Cuts at: 1454 1609 2220 2313 2387 2577 2639 3369 332
Size: 155 611 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 274 263 243 190 155
146 103 93 74 62 54 24 15
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 281 1058 3771 281
Size: 777 2713 1105
Fragments arranged by size:
2713 1105 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 803 1645 1859 2161 2542 254
Size: 549 842 214 302 381 2307
Fragments arranged by size:
2307 842 549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 223 2507 223
Size: 2284 2311
BsrFI r'CCGG_y
Cuts at: 1121 2593 2774 2983 1121
Size: 1472 181 209 2733
Fragments arranged by size:
2733 1472 209 181
BsrGI T'GTAC_A
Cuts at: 269 269
Size: 4595
BssHII G'CGCG_C
Cuts at: 474 474
Size: 4595
BssSI C'ACGA_G
Cuts at: 1177 2866 3224 1177
Size: 1689 358 2548
Fragments arranged by size:
2548 1689 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1972 1972
Size: 4595
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstEII G'GTnAC_C
Cuts at: 132 132
Size: 4595
BstXI CCAn_nnnn'nTGG
Cuts at: 324 861 1192 1215 1539 324
Size: 537 331 23 324 3380
Fragments arranged by size:
3380 537 331 324 23
BstYI r'GATC_y
Cuts at: 1 885 1181 1620 2087 2110 2445 2691 3703
Size: 884 296 439 467 23 335 246 1012
Cuts at: 3703 3789 3801 4147 1
Size: 86 12 346 449
Fragments arranged by size:
1012 884 467 449 439 346 335 296
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 117 511 1265 1511 117
Size: 394 754 246 3201
Fragments arranged by size:
3201 754 394 246
BtgI C'CryG_G
Cuts at: 752 898 1208 752
Size: 146 310 4139
Fragments arranged by size:
4139 310 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 834 2545 2726 2889 486
Size: 348 1711 181 163 2192
Fragments arranged by size:
2192 1711 348 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 1838 1175
Size: 663 3932
ClaI AT'CG_AT
Cuts at: 611 611
Size: 4595
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 1439 2180 2354 2745 2772 4117 1439
Size: 741 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 741 391 174 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 184 442 783 1006 1375 1465 2618 2828 184
Size: 258 341 223 369 90 1153 210 1951
Fragments arranged by size:
1951 1153 369 341 258 223 210 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2803 3113 3259 4405 2803
Size: 310 146 1146 2993
Fragments arranged by size:
2993 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1452 1607 1452
Size: 155 4440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1295 2420 2852 3599 3867 859
Size: 436 1125 432 747 268 1587
Fragments arranged by size:
1587 1125 747 436 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1264 1264
Size: 4595
EcoO109I rG'GnC_Cy
Cuts at: 214 328 329 1734 214
Size: 114 1 1405 3075
Fragments arranged by size:
3075 1405 114 1
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
EcoRV GAT'ATC
Cuts at: 1518 1518
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4595
FspI TGC'GCA
Cuts at: 715 2376 715
Size: 1661 2934
HaeII r_GCGC'y
Cuts at: 300 495 605 2029 2277 3299 4278 300
Size: 195 110 1424 248 1022 979 617
Fragments arranged by size:
1424 1022 979 617 248 195 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 2437 2467 2469 2499 688
Size: 32 1717 30 2 30 2784
Fragments arranged by size:
2784 1717 32 30 30 2
HincII GTy'rAC
Cuts at: 1230 1634 1230
Size: 404 4191
HindIII A'AGCT_T
Cuts at: 729 729
Size: 4595
KasI G'GCGC_C
Cuts at: 601 2025 2273 601
Size: 1424 248 2923
Fragments arranged by size:
2923 1424 248
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1349 3266 3450 1349
Size: 1917 184 2494
Fragments arranged by size:
2494 1917 184
MscI TGG'CCA
Cuts at: 1441 2356 1441
Size: 915 3680
MslI CAynn'nnrTG
Cuts at: 928 1276 1474 2584 2711 928
Size: 348 198 1110 127 2812
Fragments arranged by size:
2812 1110 348 198 127
MspA1I CmG'CkG
Cuts at: 252 404 1706 2020 2380 3393 3638 252
Size: 152 1302 314 360 1013 245 1209
Fragments arranged by size:
1302 1209 1013 360 314 245 152
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 602 2026 2274 602
Size: 1424 248 2923
Fragments arranged by size:
2923 1424 248
NcoI C'CATG_G
Cuts at: 1208 1208
Size: 4595
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NspI r_CATG'y
Cuts at: 933 1203 1648 2148 3055 933
Size: 270 445 500 907 2473
Fragments arranged by size:
2473 907 500 445 270
PciI A'CATG_T
Cuts at: 3051 3051
Size: 4595
PfoI T'CCnGG_A
Cuts at: 210 554 1355 210
Size: 344 801 3450
Fragments arranged by size:
3450 801 344
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PmlI CAC'GTG
Cuts at: 1539 1539
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 214 214
Size: 4595
PspOMI G'GGCC_C
Cuts at: 328 328
Size: 4595
PstI C_TGCA'G
Cuts at: 251 1642 2327 251
Size: 1391 685 2519
Fragments arranged by size:
2519 1391 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 252 2020 2380 252
Size: 1768 360 2467
Fragments arranged by size:
2467 1768 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1454 1609 1454
Size: 155 4440
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SanDI GG'GwC_CC
Cuts at: 214 214
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 184 442 2618 2828 184
Size: 258 2176 210 1951
Fragments arranged by size:
2176 1951 258 210
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 4112 4112
Size: 4595
SexAI A'CCwGG_T
Cuts at: 977 977
Size: 4595
SfcI C'TryA_G
Cuts at: 33 247 1638 2323 3316 3507 33
Size: 214 1391 685 993 191 1121
Fragments arranged by size:
1391 1121 993 685 214 191
SfoI GGC'GCC
Cuts at: 603 2027 2275 603
Size: 1424 248 2923
Fragments arranged by size:
2923 1424 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1333 1617 1333
Size: 284 4311
SmlI C'TyrA_G
Cuts at: 1750 2665 3157 3419 1750
Size: 915 492 262 2926
Fragments arranged by size:
2926 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 933 1648 2148 933
Size: 715 500 3380
Fragments arranged by size:
3380 715 500
StuI AGG'CCT
Cuts at: 817 1436 817
Size: 619 3976
StyI C'CwwG_G
Cuts at: 1082 1208 1729 2083 1082
Size: 126 521 354 3594
Fragments arranged by size:
3594 521 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1082 2178 2311 2770 2979 1082
Size: 1096 133 459 209 2698
Fragments arranged by size:
2698 1096 459 209 133
TatI w'GTAC_w
Cuts at: 269 4110 269
Size: 3841 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 870 902 4112 4144 870
Size: 32 3210 32 1321
Fragments arranged by size:
3210 1321 32 32
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 1331 1615 1331
Size: 284 4311
XmnI GAAnn'nnTTC
Cuts at: 1000 1000
Size: 4595
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BfrBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BstZ17I CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI