Restriction map for
pF1KE0357
(Circular) (Six-base) MAPSORT of: pF1KE0357.seq Check: 6350 from: 1 to: 4916
pF1KE0357 4916 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1954 1954
Size: 4916
Acc65I G'GTAC_C
Cuts at: 383 1932 383
Size: 1549 3367
AclI AA'CG_TT
Cuts at: 4756 4756
Size: 4916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1077 1088 1331 1756 2741 3173 3920 4188
Size: 61 11 243 425 985 432 747 268
Cuts at: 4188 1016
Size: 1744
Fragments arranged by size:
1744 985 747 432 425 268 243 61
11
AflIII A'CryG_T
Cuts at: 1386 2268 3372 4349 1386
Size: 882 1104 977 1953
Fragments arranged by size:
1953 1104 977 882
AgeI A'CCGG_T
Cuts at: 3304 3304
Size: 4916
AlwNI CAG_nnn'CTG
Cuts at: 1696 2221 3788 1696
Size: 525 1567 2824
Fragments arranged by size:
2824 1567 525
ApaLI G'TGCA_C
Cuts at: 3686 3686
Size: 4916
ApoI r'AATT_y
Cuts at: 588 597 1920 4269 4525 588
Size: 9 1323 2349 256 979
Fragments arranged by size:
2349 1323 979 256 9
AseI AT'TA_AT
Cuts at: 20 961 20
Size: 941 3975
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 683 1936 683
Size: 1253 3663
AvrII C'CTAG_G
Cuts at: 1663 1663
Size: 4916
BamHI G'GATC_C
Cuts at: 1941 1941
Size: 4916
BanI G'GyrC_C
Cuts at: 383 1932 2346 2594 2629 383
Size: 1549 414 248 35 2670
Fragments arranged by size:
2670 1549 414 248 35
BanII G_rGCy'C
Cuts at: 1930 2960 1930
Size: 1030 3886
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3575 4916 2814
Size: 761 1341 2814
Fragments arranged by size:
2814 1341 761
BclI T'GATC_A
Cuts at: 2436 2436
Size: 4916
BglII A'GATC_T
Cuts at: 424 1120 2431 4468 424
Size: 696 1311 2037 872
Fragments arranged by size:
2037 1311 872 696
BlpI GC'TnA_GC
Cuts at: 2028 2028
Size: 4916
Bme1580I G_kGCm'C
Cuts at: 2541 2634 3690 2541
Size: 93 1056 3767
Fragments arranged by size:
3767 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 805 2128 2209 2544 4688 805
Size: 1323 81 335 2144 1033
Fragments arranged by size:
2144 1323 1033 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 4916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 706 418
Size: 288 4628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2971 3463 3761 2092
Size: 879 492 298 3247
Fragments arranged by size:
3247 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4620 4620
Size: 4916
BsaAI yAC'GTr
Cuts at: 2269 2269
Size: 4916
BsaBI GATnn'nnATC
Cuts at: 6 2430 4467 6
Size: 2424 2037 455
Fragments arranged by size:
2424 2037 455
BsaHI Gr'CG_yC
Cuts at: 2347 2595 3297 4793 2347
Size: 248 702 1496 2470
Fragments arranged by size:
2470 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2105 2626 3304 3578 3725 4136 2105
Size: 521 678 274 147 411 2885
Fragments arranged by size:
2885 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1050 1080 1050
Size: 30 4886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 671 671
Size: 4916
BseYI C'CCAG_C
Cuts at: 2341 3099 3676 4172 2341
Size: 758 577 496 3085
Fragments arranged by size:
3085 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2458 2504 3712 104
Size: 2354 46 1208 1308
Fragments arranged by size:
2354 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1930 2708 2898 3690 1930
Size: 778 190 792 3156
Fragments arranged by size:
3156 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1930 2541 2634 2708 2898 2960 3690 1930
Size: 611 93 74 190 62 730 3156
Fragments arranged by size:
3156 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4136 4136
Size: 4916
BspHI T'CATG_A
Cuts at: 1163 4092 1163
Size: 2929 1987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 1704 1966 2180 2482 2863 254
Size: 1450 262 214 302 381 2307
Fragments arranged by size:
2307 1450 381 302 262 214
BsrBI CCG'CTC
Cuts at: 50 3208 3262 4746 4911 50
Size: 3158 54 1484 165 55
Fragments arranged by size:
3158 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 249 1681 1751 2828 249
Size: 1432 70 1077 2337
Fragments arranged by size:
2337 1432 1077 70
BsrFI r'CCGG_y
Cuts at: 2914 3095 3304 2914
Size: 181 209 4526
Fragments arranged by size:
4526 209 181
BsrGI T'GTAC_A
Cuts at: 1329 1329
Size: 4916
BssSI C'ACGA_G
Cuts at: 3187 3545 3187
Size: 358 4558
BstAPI GCAn_nnn'nTGC
Cuts at: 2293 2293
Size: 4916
BstBI TT'CG_AA
Cuts at: 3277 3277
Size: 4916
BstYI r'GATC_y
Cuts at: 1 424 1120 1150 1941 2408 2431 2766 3012
Size: 423 696 30 791 467 23 335 246
Cuts at: 3012 4024 4110 4122 4468 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 791 696 467 449 423 346 335
246 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1777 1777
Size: 4916
BtgI C'CryG_G
Cuts at: 107 107
Size: 4916
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4572
Fragments arranged by size:
4572 181 163
BtsI GCAGTG_nn'
Cuts at: 2159 2159
Size: 4916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 361 396 1263 1298 361
Size: 35 867 35 3979
Fragments arranged by size:
3979 867 35 35
DraI TTT'AAA
Cuts at: 339 1915 339
Size: 1576 3340
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3480 2622
Size: 858 4058
EaeI y'GGCC_r
Cuts at: 2501 2675 3066 3093 4438 2501
Size: 174 391 27 1345 2979
Fragments arranged by size:
2979 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2501 2501
Size: 4916
EarI CTCTTCn'nnn_
Cuts at: 124 526 902 1732 2939 3149 124
Size: 402 376 830 1207 210 1891
Fragments arranged by size:
1891 1207 830 402 376 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3124 3434 3580 4726 3124
Size: 310 146 1146 3314
Fragments arranged by size:
3314 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1928 1928
Size: 4916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 706 1016 1077 1088 1331 1756 2741 3173
Size: 288 310 61 11 243 425 985 432
Cuts at: 3173 3920 4188 418
Size: 747 268 1146
Fragments arranged by size:
1146 985 747 432 425 310 288 268
243 61 11
EcoO109I rG'GnC_Cy
Cuts at: 2055 2055
Size: 4916
EcoRI G'AATT_C
Cuts at: 588 1920 588
Size: 1332 3584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4916
FspI TGC'GCA
Cuts at: 2697 2697
Size: 4916
HaeII r_GCGC'y
Cuts at: 2350 2598 3620 4599 2350
Size: 248 1022 979 2667
Fragments arranged by size:
2667 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 1050 1082 2758 2788 2790 2820 409
Size: 32 609 32 1676 30 2 30 2505
Fragments arranged by size:
2505 1676 609 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1158 1955 1158
Size: 797 4119
HindIII A'AGCT_T
Cuts at: 1443 1443
Size: 4916
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 387 1936 387
Size: 1549 3367
MfeI C'AATT_G
Cuts at: 629 1628 629
Size: 999 3917
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1354 3587 3771 1328
Size: 26 2233 184 2473
Fragments arranged by size:
2473 2233 184 26
MscI TGG'CCA
Cuts at: 2677 2677
Size: 4916
MslI CAynn'nnrTG
Cuts at: 2905 3032 2905
Size: 127 4789
MspA1I CmG'CkG
Cuts at: 661 2027 2341 2701 3714 3959 661
Size: 1366 314 360 1013 245 1618
Fragments arranged by size:
1618 1366 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 107 107
Size: 4916
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NspI r_CATG'y
Cuts at: 1330 1969 2469 3376 1330
Size: 639 500 907 2870
Fragments arranged by size:
2870 907 639 500
PciI A'CATG_T
Cuts at: 3372 3372
Size: 4916
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4884
PshAI GACnn'nnGTC
Cuts at: 1244 1244
Size: 4916
PstI C_TGCA'G
Cuts at: 1701 1963 2648 1701
Size: 262 685 3969
Fragments arranged by size:
3969 685 262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4916
PvuII CAG'CTG
Cuts at: 661 2341 2701 661
Size: 1680 360 2876
Fragments arranged by size:
2876 1680 360
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 1930 1930
Size: 4916
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4916
ScaI AGT'ACT
Cuts at: 1870 4433 1870
Size: 2563 2353
SfcI C'TryA_G
Cuts at: 33 1697 1959 2644 3637 3828 33
Size: 1664 262 685 993 191 1121
Fragments arranged by size:
1664 1121 993 685 262 191
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SmaI CCC'GGG
Cuts at: 1938 1938
Size: 4916
SmlI C'TyrA_G
Cuts at: 2071 2986 3478 3740 2071
Size: 915 492 262 3247
Fragments arranged by size:
3247 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4916
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4416
StyI C'CwwG_G
Cuts at: 107 124 1663 2050 2404 107
Size: 17 1539 387 354 2619
Fragments arranged by size:
2619 1539 387 354 17
SwaI ATTT'AAAT
Cuts at: 339 339
Size: 4916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 1565 2499 2632 3091 3300 820
Size: 745 934 133 459 209 2436
Fragments arranged by size:
2436 934 745 459 209 133
TatI w'GTAC_w
Cuts at: 1329 1868 4431 1329
Size: 539 2563 1814
Fragments arranged by size:
2563 1814 539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1068 1100 4433 4465 1068
Size: 32 3333 32 1519
Fragments arranged by size:
3333 1519 32 32
Tth111I GACn'n_nGTC
Cuts at: 2713 2713
Size: 4916
XbaI T'CTAG_A
Cuts at: 592 1947 592
Size: 1355 3561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2411
Size: 4916
XmaI C'CCGG_G
Cuts at: 1936 1936
Size: 4916
XmnI GAAnn'nnTTC
Cuts at: 1786 1786
Size: 4916
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI ZraI