Restriction map for
pF1KE0347
(Circular) (Six-base) MAPSORT of: pF1KE0347.seq Check: 8982 from: 1 to: 5672
pF1KE0347 5672 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 708 2147 708
Size: 1439 4233
AccI GT'mk_AC
Cuts at: 2710 2710
Size: 5672
Acc65I G'GTAC_C
Cuts at: 2688 2688
Size: 5672
AclI AA'CG_TT
Cuts at: 5512 5512
Size: 5672
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1355 1645 1940 2665 3497 3929 4676 4944
Size: 241 290 295 725 832 432 747 268
Cuts at: 4944 1114
Size: 1842
Fragments arranged by size:
1842 832 747 725 432 295 290 268
241
AfeI AGC'GCT
Cuts at: 1699 1699
Size: 5672
AflIII A'CryG_T
Cuts at: 1508 1682 2558 3024 4128 5105 1508
Size: 174 876 466 1104 977 2075
Fragments arranged by size:
2075 1104 977 876 466 174
AgeI A'CCGG_T
Cuts at: 4060 4060
Size: 5672
AleI CACnn'nnGTG
Cuts at: 2557 2557
Size: 5672
AlwNI CAG_nnn'CTG
Cuts at: 2592 2977 4544 2592
Size: 385 1567 3720
Fragments arranged by size:
3720 1567 385
ApaI G_GGCC'C
Cuts at: 1116 2029 2304 1116
Size: 913 275 4484
Fragments arranged by size:
4484 913 275
ApaLI G'TGCA_C
Cuts at: 2511 2545 4442 2511
Size: 34 1897 3741
Fragments arranged by size:
3741 1897 34
ApoI r'AATT_y
Cuts at: 2128 2676 5025 5281 2128
Size: 548 2349 256 2519
Fragments arranged by size:
2519 2349 548 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5672
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5672
AvaI C'yCGr_G
Cuts at: 479 2326 2368 2692 479
Size: 1847 42 324 3459
Fragments arranged by size:
3459 1847 324 42
BamHI G'GATC_C
Cuts at: 2697 2697
Size: 5672
BanI G'GyrC_C
Cuts at: 1718 2578 2688 3102 3350 3385 1718
Size: 860 110 414 248 35 4005
Fragments arranged by size:
4005 860 414 248 110 35
BanII G_rGCy'C
Cuts at: 1116 1469 1670 2029 2304 2522 2686 3716 1116
Size: 353 201 359 275 218 164 1030 3072
Fragments arranged by size:
3072 1030 359 353 275 218 201 164
BbeI G_GCGC'C
Cuts at: 1722 3106 3354 1722
Size: 1384 248 4040
Fragments arranged by size:
4040 1384 248
BbsI GAAGACnn'nnnn_
Cuts at: 167 1254 167
Size: 1087 4585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 545 579 907 941 2502 2536 545
Size: 34 328 34 1561 34 3681
Fragments arranged by size:
3681 1561 328 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3570 4331 5672 3570
Size: 761 1341 3570
Fragments arranged by size:
3570 1341 761
BclI T'GATC_A
Cuts at: 3192 3192
Size: 5672
BglI GCCn_nnn'nGGC
Cuts at: 491 491
Size: 5672
BglII A'GATC_T
Cuts at: 3187 5224 3187
Size: 2037 3635
BlpI GC'TnA_GC
Cuts at: 298 2784 298
Size: 2486 3186
Bme1580I G_kGCm'C
Cuts at: 1116 1636 2029 2304 2515 2549 2583 3297 3390
Size: 520 393 275 211 34 34 714 93
Cuts at: 3390 4446 1116
Size: 1056 2342
Fragments arranged by size:
2342 1056 714 520 393 275 211 93
34 34
BmgBI CAC'GTC
Cuts at: 1989 2559 1989
Size: 570 5102
BmrI ACTGGGnnnn_n'
Cuts at: 355 955 1145 2884 2965 3300 5444 355
Size: 600 190 1739 81 335 2144 583
Fragments arranged by size:
2144 1739 600 583 335 190 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4039 4039
Size: 5672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 426 1045 1091 1639 1692 2269 2628 241
Size: 185 619 46 548 53 577 359 3285
Fragments arranged by size:
3285 619 577 548 359 185 53 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1516 2848 3727 4219 4517 1288
Size: 228 1332 879 492 298 2443
Fragments arranged by size:
2443 1332 879 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 927 5376 927
Size: 4449 1223
BsaAI yAC'GTr
Cuts at: 1980 3025 1980
Size: 1045 4627
BsaBI GATnn'nnATC
Cuts at: 6 1346 1526 3186 5223 6
Size: 1340 180 1660 2037 455
Fragments arranged by size:
2037 1660 1340 455 180
BsaHI Gr'CG_yC
Cuts at: 146 646 1709 1719 3103 3351 4053 5549 146
Size: 500 1063 10 1384 248 702 1496 269
Fragments arranged by size:
1496 1384 1063 702 500 269 248 10
BsaWI w'CCGG_w
Cuts at: 2861 3382 4060 4334 4481 4892 2861
Size: 521 678 274 147 411 3641
Fragments arranged by size:
3641 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 424 454 184
Size: 30 210 30 5402
Fragments arranged by size:
5402 210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 1945 154
Size: 1791 3881
BseYI C'CCAG_C
Cuts at: 408 592 1060 2595 3097 3855 4432 4928 408
Size: 184 468 1535 502 758 577 496 1152
Fragments arranged by size:
1535 1152 758 577 502 496 468 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1524 1220
Size: 304 5368
BsiEI CG_ry'CG
Cuts at: 104 1649 2179 3214 3260 4468 104
Size: 1545 530 1035 46 1208 1308
Fragments arranged by size:
1545 1308 1208 1035 530 46
BsiHKAI G_wGCw'C
Cuts at: 2515 2522 2549 2686 3464 3654 4446 2515
Size: 7 27 137 778 190 792 3741
Fragments arranged by size:
3741 792 778 190 137 27 7
BsmBI CGTCTCn'nnnn_
Cuts at: 540 999 540
Size: 459 5213
Bsp1286I G_dGCh'C
Cuts at: 1116 1469 1636 1670 2029 2304 2515 2522 2549
Size: 353 167 34 359 275 211 7 27
Cuts at: 2549 2583 2686 3297 3390 3464 3654 3716 4446
Size: 34 103 611 93 74 190 62 730
Cuts at: 4446 1116
Size: 2342
Fragments arranged by size:
2342 730 611 359 353 275 211 190
167 103 93 74 62 34 34 27
7
BspEI T'CCGG_A
Cuts at: 4892 4892
Size: 5672
BspHI T'CATG_A
Cuts at: 2483 4848 2483
Size: 2365 3307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 708 2147 2722 2936 3238 3619 708
Size: 1439 575 214 302 381 2761
Fragments arranged by size:
2761 1439 575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3964 4018 5502 5667 50
Size: 3914 54 1484 165 55
Fragments arranged by size:
3914 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 3584 1937
Size: 1647 4025
BsrFI r'CCGG_y
Cuts at: 148 485 1595 2114 2174 3670 3851 4060 148
Size: 337 1110 519 60 1496 181 209 1760
Fragments arranged by size:
1760 1496 1110 519 337 209 181 60
BssSI C'ACGA_G
Cuts at: 790 1584 1960 3943 4301 790
Size: 794 376 1983 358 2161
Fragments arranged by size:
2161 1983 794 376 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1547 3049 1547
Size: 1502 4170
BstBI TT'CG_AA
Cuts at: 2126 4033 2126
Size: 1907 3765
BstXI CCAn_nnnn'nTGG
Cuts at: 898 898
Size: 5672
BstYI r'GATC_y
Cuts at: 1 2528 2697 3164 3187 3522 3768 4780 4866
Size: 2527 169 467 23 335 246 1012 86
Cuts at: 4866 4878 5224 1
Size: 12 346 449
Fragments arranged by size:
2527 1012 467 449 346 335 246 169
86 23 12
BtgI C'CryG_G
Cuts at: 518 577 830 1116 2574 518
Size: 59 253 286 1458 3616
Fragments arranged by size:
3616 1458 286 253 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 338 917 1842 3622 3803 3966 338
Size: 579 925 1780 181 163 2044
Fragments arranged by size:
2044 1780 925 579 181 163
BtsI GCAGTG_nn'
Cuts at: 2915 2915
Size: 5672
DraI TTT'AAA
Cuts at: 2671 2671
Size: 5672
DraIII CAC_nnn'GTG
Cuts at: 1547 1547
Size: 5672
DrdI GACnn_nn'nnGTC
Cuts at: 361 3378 4236 361
Size: 3017 858 1797
Fragments arranged by size:
3017 1797 858
EaeI y'GGCC_r
Cuts at: 580 974 1646 2176 3257 3431 3822 3849 5194
Size: 394 672 530 1081 174 391 27 1345
Cuts at: 5194 580
Size: 1058
Fragments arranged by size:
1345 1081 1058 672 530 394 391 174
27
EagI C'GGCC_G
Cuts at: 1646 2176 3257 1646
Size: 530 1081 4061
Fragments arranged by size:
4061 1081 530
EarI CTCTTCn'nnn_
Cuts at: 382 2125 2638 3695 3905 382
Size: 1743 513 1057 210 2149
Fragments arranged by size:
2149 1743 1057 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2063 3880 4190 4336 5482 2063
Size: 1817 310 146 1146 2253
Fragments arranged by size:
2253 1817 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2520 2684 2520
Size: 164 5508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 426 1045 1091 1114 1355 1639 1645 1692
Size: 185 619 46 23 241 284 6 47
Cuts at: 1692 1940 2269 2628 2665 3497 3929 4676 4944
Size: 248 329 359 37 832 432 747 268
Cuts at: 4944 241
Size: 969
Fragments arranged by size:
969 832 747 619 432 359 329 284
268 248 241 185 47 46 37 23
6
EcoNI CCTnn'n_nnAGG
Cuts at: 682 2101 682
Size: 1419 4253
EcoO109I rG'GnC_Cy
Cuts at: 182 539 786 1146 2025 2300 2603 2811 182
Size: 357 247 360 879 275 303 208 3043
Fragments arranged by size:
3043 879 360 357 303 275 247 208
EcoRI G'AATT_C
Cuts at: 2676 2676
Size: 5672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2670 3691 2670
Size: 1021 4651
FspI TGC'GCA
Cuts at: 3453 3453
Size: 5672
HaeII r_GCGC'y
Cuts at: 1302 1701 1722 3106 3354 4376 5355 1302
Size: 399 21 1384 248 1022 979 1619
Fragments arranged by size:
1619 1384 1022 979 399 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 3514 3544 3546 3576 184
Size: 32 3298 30 2 30 2280
Fragments arranged by size:
3298 2280 32 30 30 2
HincII GTy'rAC
Cuts at: 177 2711 177
Size: 2534 3138
HindIII A'AGCT_T
Cuts at: 1657 1657
Size: 5672
KasI G'GCGC_C
Cuts at: 1718 3102 3350 1718
Size: 1384 248 4040
Fragments arranged by size:
4040 1384 248
KpnI G_GTAC'C
Cuts at: 2692 2692
Size: 5672
MluI A'CGCG_T
Cuts at: 5105 5105
Size: 5672
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 1085 1926 2588 2649 4343 4527 227
Size: 858 841 662 61 1694 184 1372
Fragments arranged by size:
1694 1372 858 841 662 184 61
MscI TGG'CCA
Cuts at: 976 3433 976
Size: 2457 3215
MslI CAynn'nnrTG
Cuts at: 131 835 2557 3661 3788 131
Size: 704 1722 1104 127 2015
Fragments arranged by size:
2015 1722 1104 704 127
MspA1I CmG'CkG
Cuts at: 412 1092 1258 1645 2783 3097 3457 4470 4715
Size: 680 166 387 1138 314 360 1013 245
Cuts at: 4715 412
Size: 1369
Fragments arranged by size:
1369 1138 1013 680 387 360 314 245
166
NaeI GCC'GGC
Cuts at: 150 487 2116 3853 150
Size: 337 1629 1737 1969
Fragments arranged by size:
1969 1737 1629 337
NarI GG'CG_CC
Cuts at: 1719 3103 3351 1719
Size: 1384 248 4040
Fragments arranged by size:
4040 1384 248
NcoI C'CATG_G
Cuts at: 577 830 577
Size: 253 5419
NgoMIV G'CCGG_C
Cuts at: 148 485 2114 3851 148
Size: 337 1629 1737 1969
Fragments arranged by size:
1969 1737 1629 337
NruI TCG'CGA
Cuts at: 267 1861 267
Size: 1594 4078
NspI r_CATG'y
Cuts at: 882 1481 1512 1686 2663 2725 3225 4132 882
Size: 599 31 174 977 62 500 907 2422
Fragments arranged by size:
2422 977 907 599 500 174 62 31
PciI A'CATG_T
Cuts at: 1508 1682 4128 1508
Size: 174 2446 3052
Fragments arranged by size:
3052 2446 174
PflMI CCAn_nnn'nTGG
Cuts at: 1023 1023
Size: 5672
PfoI T'CCnGG_A
Cuts at: 218 380 2246 2605 218
Size: 162 1866 359 3285
Fragments arranged by size:
3285 1866 359 162
PmeI GTTT'AAAC
Cuts at: 2671 2671
Size: 5672
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 5640
PpuMI rG'GwC_Cy
Cuts at: 539 786 2603 539
Size: 247 1817 3608
Fragments arranged by size:
3608 1817 247
PspOMI G'GGCC_C
Cuts at: 1112 2025 2300 1112
Size: 913 275 4484
Fragments arranged by size:
4484 913 275
PspXI vC'TCGA_Gb
Cuts at: 2368 2368
Size: 5672
PstI C_TGCA'G
Cuts at: 190 930 1748 2378 2719 3404 190
Size: 740 818 630 341 685 2458
Fragments arranged by size:
2458 818 740 685 630 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5672
PvuII CAG'CTG
Cuts at: 412 1092 1258 3097 3457 412
Size: 680 166 1839 360 2627
Fragments arranged by size:
2627 1839 680 360 166
RsrII CG'GwC_CG
Cuts at: 3867 3867
Size: 5672
SacI G_AGCT'C
Cuts at: 2522 2686 2522
Size: 164 5508
SalI G'TCGA_C
Cuts at: 2709 2709
Size: 5672
SapI GCTCTTCn'nnn_
Cuts at: 382 2125 3695 3905 382
Size: 1743 1570 210 2149
Fragments arranged by size:
2149 1743 1570 210
SbfI CC_TGCA'GG
Cuts at: 1748 2719 1748
Size: 971 4701
ScaI AGT'ACT
Cuts at: 5189 5189
Size: 5672
SexAI A'CCwGG_T
Cuts at: 535 535
Size: 5672
SfcI C'TryA_G
Cuts at: 33 186 926 1744 2374 2715 3400 4393 4584
Size: 153 740 818 630 341 685 993 191
Cuts at: 4584 33
Size: 1121
Fragments arranged by size:
1121 993 818 740 685 630 341 191
153
SfiI GGCCn_nnn'nGGCC
Cuts at: 491 491
Size: 5672
SfoI GGC'GCC
Cuts at: 1720 3104 3352 1720
Size: 1384 248 4040
Fragments arranged by size:
4040 1384 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5672
SgrAI Cr'CCGG_yG
Cuts at: 148 148
Size: 5672
SmaI CCC'GGG
Cuts at: 2328 2694 2328
Size: 366 5306
SmlI C'TyrA_G
Cuts at: 1303 1495 2368 2827 3742 4234 4496 1303
Size: 192 873 459 915 492 262 2479
Fragments arranged by size:
2479 915 873 492 459 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5672
SphI G_CATG'C
Cuts at: 882 2725 3225 882
Size: 1843 500 3329
Fragments arranged by size:
3329 1843 500
SspI AAT'ATT
Cuts at: 2013 2013
Size: 5672
StuI AGG'CCT
Cuts at: 462 1750 2096 462
Size: 1288 346 4038
Fragments arranged by size:
4038 1288 346
StyI C'CwwG_G
Cuts at: 577 830 2159 2806 3160 577
Size: 253 1329 647 354 3089
Fragments arranged by size:
3089 1329 647 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1349 3255 3388 3847 4056 1308
Size: 41 1906 133 459 209 2924
Fragments arranged by size:
2924 1906 459 209 133 41
TatI w'GTAC_w
Cuts at: 2418 5187 2418
Size: 2769 2903
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 778 810 5189 5221 448
Size: 32 298 32 4379 32 899
Fragments arranged by size:
4379 899 298 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3469 3469
Size: 5672
XbaI T'CTAG_A
Cuts at: 2703 2703
Size: 5672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3167 3167
Size: 5672
XhoI C'TCGA_G
Cuts at: 2368 2368
Size: 5672
XmaI C'CCGG_G
Cuts at: 2326 2692 2326
Size: 366 5306
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PshAI PsiI PsrI SacII SanDI SnaBI SrfI
SwaI XmnI ZraI